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Citations for Merck :
1151 - 1200 of 1360 citations for Recombinant Human CDKN1A Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... All the fractions containing the monomeric protein were pooled together and concentrated using amicon 10 k MWCO centrifugal filtration devices (Merck). Protein purity was determined by aRP to be 88.6% (Supplementary Figure 8) ...
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bioRxiv - Molecular Biology 2020Quote: ... 30~50 μg of protein samples were separated on a 10% SDS-PAGE gel and transferred to a PVDF membrane (Merck) using an Amersham semi-dry transfer system ...
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bioRxiv - Cancer Biology 2020Quote: ... The membrane was incubated with secondary antibody for 1 hour at room temperature and proteins were detected using chemiluminescent HRP substrate (Merck-Millipore ...
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bioRxiv - Systems Biology 2022Quote: ... Protein precipitation was performed by addition of four volumes of ice-cold acetone (−20°C) (Merck Sigma, Cat. No. 650501) or TCA to a final concentration of 20% and proceeded for 1 hour at -20°C or 30 min at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... The His6-tagged proteins were purified using Immobilised Metal Affinity Chromatography (IMAC) with Ni-NTA His•Bind® Resin (Merck). Fractions containing the protein of interest were then dialysed against low-salt (50mM Tris pH8.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The His6-tagged proteins were purified using Immobilised Metal Affinity Chromatography (IMAC) with Ni-NTA His•Bind® Resin (Merck). Fractions containing the protein of interest were then dialysed against low-salt buffer (50mM Tris pH7.0 ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was filtered using Millex Non-Sterile Low Protein Binding Hydrophilic LCR (PTFE) Membrane (0.45 µm) filters (Merck #SLLHR04NL) into 1.5 mL MS vials with silicone/PTFE septa and stored at -20 °C for later analysis.
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bioRxiv - Microbiology 2021Quote: ... Cell lysates were loaded and resolved using a 10% SDS-PAGE gel and the separated proteins were transferred onto a PVDF membrane (IPVH00010, Immobilon-P; Merck). Blocking was performed using 5% Skimmed milk (70166 ...
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bioRxiv - Biochemistry 2021Quote: ... varieornatus CAHS1 protein was constructed and cloned as a fusion protein with a hexahistidine (His6) tag at the N-terminus using a pET28a vector (Novagen, Merck). CAHS1-N (Met1-Ser96 ...
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bioRxiv - Cancer Biology 2021Quote: ... at 4°C and the supernatant was taken for determining protein concentrations using a Direct Detect® Infrared Spectrometer (Merck). Whole lysate was used for subsequent analysis ...
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bioRxiv - Microbiology 2022Quote: ... The clear cell-free extracts were immunoprecipitated using monoclonal antibodies against T18 tag following Protein G Immunoprecipitation Kit (Merck Inc) protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... Fractions containing protein were pooled and concentrated using Amicon Ultra-15 centrifugal filter unit (10-kDa molecular mass cut-off; Merck) until total protein concentration of 10 mg·mL−1 was reached ...
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bioRxiv - Biophysics 2019Quote: ... Protein contaminants were eliminated by subjecting the combined IF1 fractions to Amicon® Ultra 30K Da centrifugal filters (Merck, Germany) followed by concentration on a HiTrap™ SP HP (GE Healthcare Life Sciences ...
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bioRxiv - Biophysics 2019Quote: ... All the fractions containing the monomeric protein were pooled together and concentrated by using amicon 10 k MWCO centrifugal filtration devices (Merck).
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bioRxiv - Cancer Biology 2019Quote: ... Subsequently 20 μg of protein were separated by SDS-polyacrylamide gel electrophoresis and transferred onto a polyvinylidene difluoride membranes (Immobilon-P PVDF, EMD, Merck,). Membranes were blocked with 5% fat-free milk in TBST (0.01% Tween ...
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bioRxiv - Neuroscience 2019Quote: ... The HiTrap SP elution fractions containing the tau proteins were concentrated using a 30 MWCO or 3 MWCO Amicon centrifugal filter unit (Merck) and loaded on a HiLoad 16/600 Superdex 75 pg size exclusion chromatography column (GE Healthcare ...
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bioRxiv - Microbiology 2019Quote: Proteins in cell lysates were separated in a 10% SDS-PAGE and transferred to Immobilon NC (Millipore Merck, Darmstadt, Germany) membranes ...
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bioRxiv - Cancer Biology 2020Quote: ... 3 μg proteins were separated by SDS-PAGE and electrotransferred onto Immobilon®-P transfer membranes (Merck Millipore, MA, USA), blocked with 5% skim milk in Tris-buffered saline with 5% Tween 20 (TBST) ...
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bioRxiv - Cell Biology 2020Quote: ... alkylated with 40 mM Chloroacetamide (CAA) at RT for 30 min and protein amount was quantified using the Direct Detect Spectrometer from Merck. Protein digestion was performed using the Single-Pot Solid-Phase-enhanced Sample Preparation approach SP3 ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 mg of total protein at a 1 mg/ml concentration was incubated with 40 µl anti-FLAG-M2 affinity resin (Merck) (which had been blocked overnight with 1% BSA and 5 µl/ml ssDNA ...
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bioRxiv - Cancer Biology 2019Quote: ... The protein was visualized by exposing the membrane to an X-Omat film with enhanced chemiluminescence reagent (Merck, Darmstadt, Germany). The respective primary antibodies were mouse anti-DRP1 ...
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Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptorbioRxiv - Biochemistry 2021Quote: ... all proteins were centrifuged (16 000 g, 10 min, 20°C) and filtered (0.1 μm Ultrafree-MC filter, Merck Millipore). Proper performance of the MALS detectors was validated with bovine serum albumin ...
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bioRxiv - Cell Biology 2021Quote: ... Chromatin and its associated proteins were solubilized by incubation with 4- 20 units of Micrococcal nuclease (MNase; Merck Life Sciences) for 10 minutes at 37° C ...
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bioRxiv - Cancer Biology 2020Quote: ... The glutathione-S-transferases (GST) fusion proteins were expressed in Escherichia coli and purified with glutathione-conjugated agarose beads (Merck) by standard procedures ...
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bioRxiv - Cancer Biology 2020Quote: ... The protein was collected from the supernatant of cultured cells and concentrated by Amicon Ultra-15 Centrifugal Filter Unit (Merck). Then the fusion protein was purified using BeaverBeads IDA-Nickel Kit (Beaverbio) ...
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bioRxiv - Biochemistry 2021Quote: ... 50 µg of protein solution was mixed with dye at a three-fold molar excess in the presence of NaHCO3 (Merck) buffer at pH 8.3 ...
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bioRxiv - Immunology 2020Quote: ... The proteins were then separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred onto an Immobilon-P membrane (IPVH00010; Merck). The membranes were blocked with 10% nonfat milk (190-12865 ...
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bioRxiv - Cancer Biology 2022Quote: ... Equal amounts of protein (10 µg) were analyzed by SDS-PAGE and transferred to Immobilon-FL PVDF membranes (Merck Millipore) using Pierce™ 1-step transfer buffer (Thermo ...
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bioRxiv - Biochemistry 2022Quote: ... lysates were incubated with anti-HA agarose beads (20 μl of beads per 1 mg of proteins) (Merck, Darmstadt, Germany) for 2 h ...
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bioRxiv - Biochemistry 2022Quote: ... The MTF1 protein eluent was collected and concentrated using 10 kDa MW cut-off Amicon ultra-centrifugal filter (Merck Millipore) and stored at -80°C ...
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bioRxiv - Cell Biology 2022Quote: Proteins (either from whole cell lysates or insoluble fraction) were resolved by SDS-PAGE and transferred to PVDF membrane (Merck) by western blotting ...
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bioRxiv - Immunology 2022Quote: ... an aliquot of the sample was taken for measuring the total protein concentration using a BCA assay kit (Merck Millipore). The remaining sample was heat-treated (70 °C ...
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bioRxiv - Plant Biology 2022Quote: ... Protein samples were concentrated by centrifugation using spin columns (MW cut off of 10 kDa) (cat number UFC501096– Merck Millipore). Protein concentration was estimated by UV using calculated extinction coefficients (42,400 mol/l-1.cm-1) ...
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bioRxiv - Plant Biology 2022Quote: ... 1 mL of filtered protein extract was mixed with 40μL of anti-FLAG M2 magnetic beads (Merck, formerly Sigma-Aldrich) (equilibrated in GTEN + 0.1 % Tween-20 prior to use ...
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bioRxiv - Microbiology 2022Quote: ... The proteins were separated in a 10% SDS-polyacrylamide gel and transferred to Immobilon NC membranes (Millipore Merck, Darmstadt, Germany). The membranes were blocked with 5% non-fat dry milk in PBS for 1 h at room temperature and then incubated with primary antibodies (DB 1:2000 ...
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bioRxiv - Plant Biology 2023Quote: ... Transfection with plasmids carrying the coding sequences of the indicated proteins was performed using Genejuice transfection reagent (Merck, Darmstadt, Germany). 48 hours after transfection ...
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bioRxiv - Biophysics 2024Quote: ... The protein was concentrated to 60 µM – 100 µM using an Amicon Ultra Centrifugal Filter Unit (30 kDa MWCO, Merck) and stored at −80 °C.
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bioRxiv - Microbiology 2024Quote: ... Proteins in the lysate were then fractionated by SDS-PAGE and transferred to a PVDF membrane (Merck Millipore, Cat#IPVH00010). After blocking with 1% nonfat milk overnight at 4°C ...
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bioRxiv - Plant Biology 2024Quote: ... the purified protein was concentrated by centrifugation in 3 kDa cut-off filters (Amicon Ultra-15 Centrifugal Filter Units, Merck) at 4,500 x g ...
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bioRxiv - Cell Biology 2023Quote: ... The protein concentration was quantified and 100ug proteins of each sample were incubated in acetonitrile with Sera-Mag SpeedBead Carboxylate-Modified Magnetic Particles (Hydrophilic) (#GE44152105050250, Merck) and Sera-Mag SpeedBead Carboxylate-Modified Magnetic Particles (Hydrophobic ...
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bioRxiv - Cell Biology 2023Quote: ... Total IgG were isolated from post-immune sera by affinity purification on Sepharose-protein G (Merck, Cytiva 17-0618-05). Custom sheep pAb anti-pS55 and anti-pS44 NDC80 pAb was raised by the Scottish Blood Transfusion Service using a 4-month long program ...
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bioRxiv - Neuroscience 2023Quote: ... Protein samples were digested sequentially with Lys-C and trypsin on Microcon-10 filters (Merck Millipore, #MRCPRT010 Ultracel YM-10). The mixture was centrifuged at 1000 rcf for 5 min ...
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bioRxiv - Cancer Biology 2023Quote: ... The amount of puromycin incorporated into nascent peptides was then evaluated by Western blot analysis on 20 μg of proteins using anti-puromycin antibody (ref MABE343) purchased from Merck Millipore (Darmstadt ...
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bioRxiv - Microbiology 2023Quote: ... and lysed in SDS sample buffer containing 100 mM DTT before protein separation on precast Bis-Tris polyacrylamide gels (MPAGE; Merck) and transferred to nitrocellulose membranes by electroblotting ...
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bioRxiv - Microbiology 2023Quote: ... binding of equine antibodies was detected by incubation for 1 hour with protein A conjugated to peroxidase (GE) and visualized with chemiluminescence reagent (ECL, Merck). Images were obtained with an Odyssey LI-COR instrument ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were separated on a 10% polyacrylamide gel and transferred to polyvinylidene difluoride membrane (Amershan Hybond P PVDF membrane, Merck). Membranes were blocked in 5% milk in PBS/0.1% Tween-20 ...
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bioRxiv - Biophysics 2023Quote: ... Homogenization of proteins and single molecules were performed using 8 U/ml proteinase K (PK, #P4850 Sigma Aldrich now Merck) in digestion buffer (800 mM guanidine HCl ...
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bioRxiv - Biochemistry 2023Quote: ... 40 μg of protein was added to each well and run in Boric acid-Tris buffer (192 mM Boric acid, Merck; 1 mM EDTA ...
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bioRxiv - Cell Biology 2023Quote: ... 250 µg of protein were incubated with 25 µl of pre-equilibrated anti-FLAG resin (M2 anti-FLAG beads, Merck) on a rotator at 4°C overnight ...
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bioRxiv - Physiology 2023Quote: ... and 20 µg protein extracts were separated with 10-15% SDS-PAGE gel and transferred to the PVDF membranes (Millipore ISEQ00010, Merck). Each membrane was blocked and slit into two parts ...