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Citations for Merck :
1101 - 1150 of 1367 citations for Recombinant Human APCS Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The bacterial pellet was suspended in 5 mL lysis buffer (BugBuster Protein Extraction Reagent, Merck Millipore, UK; containing DNAse) and incubated at room temperature for 30 mins ...
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bioRxiv - Biochemistry 2020Quote: ... The bacterial pellet was suspended in 5 mL lysis buffer (BugBuster Protein Extraction Reagent, Merck Millipore, UK; containing DNAse) and incubated at room temperature for 30 mins ...
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bioRxiv - Genomics 2022Quote: ... Total chromatin was pre-cleared for 1h at 4°C with rotation using protein A sepharose beads (P9424, Merck). 3 µg of anti-histone H3 (Abcam ...
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bioRxiv - Immunology 2020Quote: ... Collected proteins were buffer exchanged into PBS via multiple rounds of centrifugation in 10 kDA ultrafiltration tubes (Merck, USA). The purified protein content was measured by Bradford assay (B6916 ...
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bioRxiv - Immunology 2020Quote: ... 20 μg of brain lysate was examined by SDS-PAGE and proteins were blotted on PVDF membrane (Immobilon, Merck). Membrane was blocked with 2% skim milk and subsequently incubated with anti-DJ-1 antibody overnight ...
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bioRxiv - Immunology 2020Quote: ... and the fractions with the target protein were mixed and concentrated using Amicon Ultra-15 Centrifugal filters (Merck Millipore) with a 3 kDa membrane cutoff (Figure 10).
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bioRxiv - Developmental Biology 2021Quote: ... We used the following primary antibodies: rabbit antibody against Protein Kinase Cα diluted at 1:500 (Antibody Registry ID: AB_477345, Merck), rabbit antibody against Recoverin at 1:1000 (AB_2253622 ...
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bioRxiv - Immunology 2021Quote: ... the protein content of the supernatants was concentrated 10x using centrifugal filters with 10 kDa cutoff (#UFC501096, Amicon, Merck). The concentrated samples were boiled in a reducing sample buffer and separated in 4-12% polyacrylamide gel (#NW04122BOX ...
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bioRxiv - Cancer Biology 2020Quote: ... Lysates were collected at 20,000 x g for 40 min at 4°C and soluble proteins in the supernatant were sterile filtered 5µm PVDF Membrane (Merck).
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bioRxiv - Microbiology 2021Quote: ... proteins were separated using a 10% SDS-PAGE gel and transferred onto a PVDF Immobilon-P membrane (Merck Millipore). The membrane was blocked with 5% w/v milk in TBST (50 mM Tris-HCl pH 7.6 ...
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bioRxiv - Microbiology 2022Quote: ... and protein expression was induced O/N with 0.5 mM isopropyl 1-thio ß-D-galactopyranoside (IPTG) (Merck Chemicals). The next day ...
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bioRxiv - Microbiology 2022Quote: ... Different amount of protein samples were electrophoresed on 10% SDS-PAGE and then transferred to PVDF membranes (Millipore, Merck) by electroblotting ...
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bioRxiv - Developmental Biology 2022Quote: ... Total protein concentration of lysates was determined using a bicinchoninic acid kit (Merck Life Science UK Ltd, Gillingham, UK) and samples diluted in Laemmli buffer ...
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bioRxiv - Plant Biology 2024Quote: ... The proteins were then transferred from the gel to a PVDF membrane (Immonilon-P transfer membrane, Merck, Darmstadt, Germany) using the wet transfer method ...
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bioRxiv - Developmental Biology 2024Quote: ... Protein pellets were resuspended in 1x S-Trap lysis buffer (5% SDS, 50 mM TEAB from Merck, pH 8.5). 50 µg of the sample protein was digested using the S-trap micro spin column digestion protocol from ProtiFi LLC ...
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bioRxiv - Cancer Biology 2023Quote: ... Supernatant was collected as control and the beads were thoroughly washed 3 x with TBS/Tween 0.2% after which bound proteins were eluted by adding hot reducing SDS sample buffer containing 2-mercaptoethanol (Merck) and boiling for 5 min at 99 °C ...
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bioRxiv - Immunology 2024Quote: ... 50 μL/well of plex beads were distributed in a MultiScreenHTS BV Filter Plate (1.2 μm Hydrophilic Low Protein Binding Durapore Membrane, Merck) and washed three times with 120 μL of ABE buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... protein concentration was determined by IR spectrometry measuring amide bond absorbance using a Direct Detect IR Spectrometer (Merck KGaA). If protein concentration (of the undiluted lysate ...
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bioRxiv - Molecular Biology 2023Quote: ... Peak fractions containing SETDB1 Triple Tudor protein were pooled and concentrated to 2 mL in Amicon Ultra-15 concentrators 10,000 molecular weight cut-off (Merck Millipore ...
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bioRxiv - Cell Biology 2023Quote: ... Separated proteins on the gels were blotted on Amersham™ Hybond® low-fluorescence 0.2 µm PVDF membranes (Merck). The membranes were blocked using fluorescence blocking buffer (Azure Biosystems ...
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bioRxiv - Molecular Biology 2023Quote: ... Citrullination of proteins was induced via activation of PADI4 by the addition of calcium ionophore Af23187 (cat. C7522, Merck) to a final concentration of 4 µM ...
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bioRxiv - Biochemistry 2023Quote: ... The eluted protein was concentrated and using using Amicon Ultra-15 centrifugal filters (3 kDa cut-off, Merck-Millipore) and then buffer was exchanged to buffer B (50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cell Biology 2023Quote: ... Expression of mScarletI-PXVam7 from the pCUP1 promoter or of down-regulated proteins from the weak pCUP1(dim) promoter was induced by supplementation of 10μM (unless noted otherwise) copper sulfate (Merck) to the growth medium ...
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bioRxiv - Biophysics 2023Quote: ... Protein extracts (50 μg/lane) were resolved in 15% SDS-PAGE followed by immunoblotting using anti-GFP (AB3080P, Merck), anti-PrPC monoclonal antibody (anti-CD320 clone 6D11 ...
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bioRxiv - Biochemistry 2023Quote: ... Protein complex-containing fractions were collected and concentrated using 10 kDa molecular weight cut-off centrifugal filters (Merck Millipore). The concentrated protein was split into two samples ...
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bioRxiv - Physiology 2023Quote: ... For citrate synthase activity, 15-30 µg protein was added to the reaction buffer (20 mM Tris-HCl, pH 8.0 (Merck), 0.42 mM acetyl-coenzyme A (Sigma) ...
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bioRxiv - Plant Biology 2023Quote: ... Protein content of supernatant samples were normalized and analyzed using SDS-PAGE (mPAGE Bis-Tris 8%, Merck, Darmstadt, Germany) revealing specific protein bands in the supernatant while comparing wildtype and the T2SS mutant (xpsxcs ...
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bioRxiv - Plant Biology 2023Quote: ... Purified proteins were concentrated to 10–30 mg/ml by using a 10-kDa Amicon centrifugal filter device (Merck), flash-frozen in liquid nitrogen ...
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bioRxiv - Cancer Biology 2023Quote: Proteins were extracted by RIPA lysis buffer (EcoTech Biotechnology, RIPA-100) containing cOmplete™ protease inhibitor cocktail (Merck, 11697498001), PMSF (Merck ...
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bioRxiv - Bioengineering 2023Quote: ... with the resulting protein pellet resuspended in Tris buffer and sterilized using 0.45 μm and 0.2 μm syringe filters (Merck, US).
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bioRxiv - Cell Biology 2023Quote: ... The bound protein complexes were washed thrice with NETN buffer and then eluted with 2 mg/mL biotin (Merck) for 90 min at 4°C ...
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bioRxiv - Plant Biology 2023Quote: ... The resin was pelleted as above and washed twice with 0.8 mL plant extraction buffer (see plant protein extraction section above) supplemented with 10 μM MG132 (Merck). Cold plant extract was either added directly to the resin (Supplementary Dataset S1 ...
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bioRxiv - Biochemistry 2023Quote: ... The eluted protein was concentrated using a centrifugal filter unit (Amicon Ultra, 10 or 30 kDa cut-off, Merck), and the solvent was exchanged with 50 mM potassium phosphate buffer (pH 7.0 ...
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bioRxiv - Biochemistry 2023Quote: ... The proteins were reduced utilizing a 5 mM solution of tris-2-carboxyethyl-phosphine (TCEP) (Merck KGaA, Darmstadt, Germany) at a temperature of 60 °C for 1 hour ...
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bioRxiv - Biochemistry 2023Quote: ... was performed on 4 and 2.5 mg protein lysates for timsTOF optimization and dual-probe analyses respectively in 2 M urea (Merck) in 1× 50 mM HEPES (pH 7.5) ...
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bioRxiv - Cancer Biology 2024Quote: Protein solutions were first diluted with 50 mM ammonium bicarbonate (ABC) and reduced with 5 mM dithiothreitol (DTT, Merck) at 60 °C for 45 min ...
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bioRxiv - Microbiology 2024Quote: The culture supernatants containing the secreted proteins were centrifugated (1 h, 30,000xg, 10 °C) and sterilized by filtration (0.22 µm; Express Plus; Merck Millipore). The supernatants were equilibrated to pH 7 with 0.5 mM NaOH and the proteins were purified using two ion exchange chromatographies (Fig ...
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bioRxiv - Microbiology 2024Quote: ... for normalization of protein loading following which western blotting was performed using an anti-HA antibody (H9658 Merck, UK). HA tagged protein bands were visualised using the LI-COR Odyssey Clx system with LI-COR secondary antibody (IRDye 800 CW anti-mouse ...
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bioRxiv - Developmental Biology 2024Quote: Total protein was extracted from cells or tissues by RIPA buffer supplemented with a protease inhibitor cocktail (Merck, #11836170001) and phenylmethanesulfonyl fluoride (Beyotime ...
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bioRxiv - Molecular Biology 2024Quote: ... immunoprecipitations performed by incubating with 8 μl Protein G Dynabeads (Novex) and 12 μl anti-FLAG antibody (F3165, Merck) per sample for 90 min at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... After 2 hours at 30°C with shaking 1 ml of cells was pelleted and soluble proteins were extracted using BugBuster (Merck). For internalisation experiments involving plasmid-complemented deletion strains ...
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bioRxiv - Genetics 2021Quote: ... For western blotting the proteins were resolved in SDS-polyacrylamide gels and transferred electrophoretically at room temperature to PVDF membranes (Merck) for 1 h at 50 mA using a Tris-glycine buffer system ...
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bioRxiv - Genetics 2021Quote: ... Prior to EMSAs the in vitro translations of Rbpj (WT) and mutant proteins were monitored by western blotting using an anti-Flag antibody (M5, Merck).
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bioRxiv - Molecular Biology 2021Quote: ... The blots were incubated with primary antibodies to the following proteins overnight at 4 °C: (1) CTCF (1:1,000; 07-729, Merck Millipore), (2 ...
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bioRxiv - Cell Biology 2022Quote: ... The total concentration of proteins in samples was determined using the Bicinchoninic Acid Assay Kit (Sigma Aldrich/Merck, Poznan, Polska). The samples containing 20 µg of protein were mixed with 4× Laemmli loading buffer (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... The eluted NlGr7 protein fractions were applied on a 10 kDa cut-off Centricon centrifugal device (Merck Pte. Ltd., Germany) for desalting against buffer (25 mM Tris–HCl ...
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bioRxiv - Molecular Biology 2020Quote: ... The cleaved NlGr7 protein was concentrated by using a 10 kDa cut-off Centricon centrifugal device (Merck Pte. Ltd., Germany) and was purified by affinity chromatography on amylose resin (NEB ...
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bioRxiv - Biophysics 2022Quote: ... and monomeric protein was purified by size-exclusion chromatography using a HiLoad™ 26/600 Superdex™ 75 pg (Merck) equilibrated with 25 mM HEPES ...
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bioRxiv - Biophysics 2019Quote: ... lyophilized protein was dissolved in sodium phosphate buffer (pH 7.4) and filtered using 0.22 μm low protein binding membranes (Millex-GP, Merck Millipore, Germany). The protein concentration was determined by the measurement of absorbance at 277 nm using the extinction coefficient 5960 cm-1M-1.
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bioRxiv - Molecular Biology 2019Quote: ... RIP-Seq experiments with UV cross-linking were performed using the Magna RIPTM RNA-Binding Protein Immunoprecipitation Kit (17-700 Merck). Briefly ...