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Citations for Merck :
801 - 850 of 1574 citations for QuantiChrom Protein Creatinine Ratio Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... An assay volume of 150 μl was dispensed into a Hellma 10 x 2 mm Suprasil quartz cuvette (Merck #Z802778). For double-stranded substrates ...
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bioRxiv - Immunology 2021Quote: ... and filtered through a 0.22 µM membrane The concentration of purified RBD was determined using the Bradford assay (Merck, Germany).
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bioRxiv - Biophysics 2021Quote: ... This mix included 98% unlabeled recombinant human E254N tubulin diluted in assay buffer containing oxygen scavengers (180 mg/ml catalase (C40, Merck) and 752 mg/ml glucose oxidase (22778.01 ...
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bioRxiv - Neuroscience 2022Quote: ... 100 μL/well of the MW1 antibody coupled with magnetic particles (appropriately diluted in Erenna Assay buffer catalog #02-0474-00; Merck), were added to the assay plate ...
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bioRxiv - Biochemistry 2024Quote: ... H2O was removed and ROS production was measured upon addition of a 100-μL assay solution which contained 10 μg/mL horseradish peroxidase (P6782, Merck), 10 mM luminol and the treatment (1 μM SCOOP ...
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bioRxiv - Cancer Biology 2023Quote: Tumorspheres formation assay was performed by seeding transfected cells in Corning Costar ultra-low attachment plates (#CLS3473, Merck, Madrid, Spain) in DMEM F-12 medium (#11320033 ...
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bioRxiv - Neuroscience 2023Quote: The viability of SH-SY5Y cells following incubation with fibrils formed in the absence/presence of gangliosides was determined using a standard MTT cell proliferation assay (Merck).
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Gliflozins, sucrose and flavonoids are allosteric activators of lecithin:cholesterol acyltransferasebioRxiv - Biochemistry 2024Quote: ... PAM (0.1 mg/mL in DMSO) or DMSO were incubated with LCAT (5 μg/mL in assay buffer + 60 μM albumin (Merck)) 1:100 v/v at 37°C gently shaken ...
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bioRxiv - Neuroscience 2024Quote: Samples of 2mm diameter retinal punches were collected from donor eyes (Supplementary Figure 1A) and homogenized on ice using radio-immunoprecipitation assay (RIPA) buffer (#900000064244, Merck) supplied with phosphatase and protease inhibitor (1:100 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The pure proteins were concentrated using a 3000-high molecular weight cutoff centrifugal filter (Merck-Millipore). The concentrated proteins were then subjected to size-exclusion chromatography using a Superdex-75 GL column (GE healthcare pre-equilibrated with 25 mM Tris-HCl 6.8 ...
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bioRxiv - Molecular Biology 2021Quote: ... Total protein stain (30% methanol, 6.7% acetic acid, 0.0005% Fast Green FCF (Merck KGaA, Darmstadt, Germany)) stain was used as a loading control and imaged prior to blocking ...
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bioRxiv - Biophysics 2019Quote: ... Protein was transferred overnight at 10 V to an Immobilon-P membrane (Merck Millipore; Burlington, MA) in 25 mM Tris ...
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bioRxiv - Cell Biology 2019Quote: ... Supernatant was collected and protein concentration was determined using the Direct Detect® Spectrometer (Merck Millipore). The proteins (30 µg ...
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bioRxiv - Cancer Biology 2019Quote: The uncharged polystyrene 96-well plate were coated with different recombinant proteins-Collagen IV (Merck Millipore), P-Selectin ...
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bioRxiv - Bioengineering 2019Quote: ... proteins were separated by SDS-PAGE and transferred onto PVDF (polyvinylidene fluoride) membranes (Merck Millipore, DE). The membranes were then blocked for 1 h in 5% non-fat milk in PBST and incubated with CD64 and CD16 antibodies and β-actin antibody respectively overnight at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... Flag-tagged proteins were eluted by incubation with 150 μg/mL Flag tag peptide (Merck; F3290) with 0.1% digitonin in solubilisation buffer for 30 min at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... Protein samples were transferred and western blotted using anti phospho-T187 p27KIP1 antibody (Merck, 06-996). Band intensity was revealed using ECL reagent (BioRad ...
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bioRxiv - Neuroscience 2021Quote: ... downward capillary action was used to transfer protein to immune-blot PVDF membrane (Merck cat# IPVH00010). Membranes were blocked with 5% milk in wash buffer (0.05% Tween in PBS ...
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CAP1 and cofilin1 cooperate in neuronal actin dynamics, growth cone function and neuron connectivitybioRxiv - Neuroscience 2020Quote: ... Equal protein amounts were separated by SDS PAGE and blotted onto a polyvinylidene difluoride membrane (Merck) by using a Wet/Tank Blotting System (Biorad) ...
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bioRxiv - Immunology 2022Quote: ... Protein was digested by incubating membrane at 50°C for 1 h with proteinase K (Merck) diluted in PK-SDS buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... the protein-containing fractions were dialyzed in a cellulose membrane tube (14 kDa MWCO, Merck-Millipore), first ...
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bioRxiv - Microbiology 2022Quote: Wild type and mutant CANC proteins were expressed in Escherichia coli Rosetta (DE3) cells (Merck Millipore) as previously described (Betancor et al. ...
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bioRxiv - Neuroscience 2021Quote: ... Purified protein was concentrated to 8 mg/mL using 30 kDa cut-off AMICON concentrators (Merck).
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Loss of TREM2 reduces hyperactivation of progranulin deficient microglia but not lysosomal pathologybioRxiv - Neuroscience 2021Quote: ... Proteins were separated by SDS-PAGE and transferred onto polyvinylidene difluoride membranes (Immobilon-P, Merck Millipore). Proteins of interest were detected using the following primary antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were transferred to a PVDF membrane (0.45µm Immobilon®-P, Merck Millipore Ltd., VIC, Australia) at 100 V in chilled transfer buffer containing 25 mM Tris ...
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bioRxiv - Neuroscience 2020Quote: ... and mouse monoclonal IgG2A antibody to myelin Proteolipid Protein (clone PLPC1, MAB388, MERCK-Millipore, 1:100). All slides were mounted using Mowiol mounting medium (475904 ...
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bioRxiv - Immunology 2022Quote: ... Proteins were resolved on SDS–PAGE gels and transferred to polyvinylidene difluoride (PVDF) membranes (Merck Millipore). The PVDF membranes were then blocked with 5% skim milk and sequentially incubated with primary and secondary antibodies ...
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bioRxiv - Biochemistry 2019Quote: All proteins were expressed in E.coli Rosetta-2 DE3 cells (EMD Millipore, Merck KGaA, Darmstadt, Germany). Cells were transformed via heat shock (42°C for 35 secs ...
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bioRxiv - Molecular Biology 2021Quote: ... The protein was concentrated to ~65 mM using a MWCO 30,000 Amicon Ultra Centrifugal Filter (Merck), flash-frozen and stored at −80 °C until use.
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bioRxiv - Microbiology 2020Quote: Viral proteins were separated by SDS-PAGE and transferred to polyvinylidene disulfide (PVDF) membranes (Merck Millipore). After blocking the membranes with 5% skim milk ...
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bioRxiv - Plant Biology 2019Quote: ... Cells were pelleted by centrifugation and lysed in Bugbuster™ protein extraction reagent (Merck; Darmstadt, Germany) by continuous agitation for 20 min at room temperature ...
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bioRxiv - Biochemistry 2020Quote: Purified proteins were concentrated using Amicon® Ultra-4 Ultracel®-10K (MWCO: 10.000 Da; Merck). Protein concentrations were quantified using the calculated molar extinction coefficient ε280 (29).
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bioRxiv - Cell Biology 2021Quote: ... Purified proteins were were concentrated > 60 μM using Amicon Ultra centrifugal filters (Merck; MWCO 3 kDa), rebuffered to 50 mM Tris pH 7.5 ...
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bioRxiv - Plant Biology 2021Quote: ... The collected protein was concentrated using Amicon Ultra-15 Centrifugal Filter Units (Merck Millipore, Beijing China) with 100 kDa cut off and frozen at −80 °C.
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bioRxiv - Microbiology 2021Quote: ... Protein samples for mass spectrometry were purified and concentrated using ZipTipC-18 (Merck, Millipore, Darmstadt, Germany).
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bioRxiv - Microbiology 2021Quote: ... Protein signals on the membranes were detected with Immobilon Forte Western HRP substrate (Merck, Darmstadt, Germany) and the ChemiImager Ready (Alpha Innotec,San Leandro USA).
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bioRxiv - Biochemistry 2021Quote: ... Proteins were eluted with 50 μl of 100 μg/mL of 3X FLAG tag peptide (Merck) in solubilization buffer followed by acetone precipitation (overnight ...
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bioRxiv - Biochemistry 2021Quote: ... 500 μg protein was added to spin columns (Pierce) containing anti-FLAG M2 affinity resin (Merck) and allowed to incubate rotating for two hours at 4°C ...
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bioRxiv - Biophysics 2022Quote: ... Proteins were concentrated using a centrifugal filter unit (3 kDa MWCO, Merck Millipore; Cat. No. UFC900324) at 3500 rpm and 4°C ...
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SMDT1 variants impair EMRE-mediated mitochondrial calcium uptake in patients with muscle involvementbioRxiv - Genetics 2022Quote: ... proteins were transferred to a 0.45 μm PVDF membrane (#IPVH85R; Sigma-Aldrich/Merck, Zwijndrecht, The Netherlands) using an electroblotting device (Bio-Rad ...
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bioRxiv - Plant Biology 2022Quote: ... and the protein was concentrated in a centrifugal concentrator (Amicon MWCO 3 kDa: Merck, Darmstadt, Germany) and stored at −20 °C in dialysis buffer (150 mM NaCl ...
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bioRxiv - Plant Biology 2022Quote: ... The separated proteins were transferred to polyvinylidene difluoride membranes (Immobilon-P; Merck Millipore, Burlington, MA, USA) and analyzed with the following antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were concentrated by using the Amicon Ultra centrifugal device (NMWL: 3K) (Merck, Millipore Cat# UFC500324), and the concentration was determined by the Pierce BCA protein assay kit (Thermo Fisher Scientific Cat# 23225) ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant proteins were obtained after expression at 22°C in B834 (DE3) cells (Merck, Darmstadt, Germany), upon induction with 0.6□mM IPTG at 0.8 OD600 ...
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bioRxiv - Biophysics 2022Quote: ... The pure protein obtained was concentrated using a 3 kDa Amicon® Ultra Centrifugal Filter (Merck).
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bioRxiv - Molecular Biology 2024Quote: ... and for miniTurbo–TAF protein expression using a rat anti-HA-HRP antibody (3F10, Merck, 12013819001). The protein concentration was determined with Protein Assay Dye Reagent Concentrate (Biorad ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were transferred onto methanol pre-activated polyvinylidene fluoride (PVDF) membranes (IPFL00010, Immobilon-P; Merck-Millipore). Membranes transferred from TGX Stain-Free™ gels were imaged and quantified for posterior normalization with a ChemiDoc XRS+ (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was filtered through a 0.45 μm low protein binding durapore membrane (Merck Millipore Ltd.), and the progeny virus stock was aliquoted and stored at −20°C until use.
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bioRxiv - Biochemistry 2023Quote: ... Fractions containing full-size protein were concentrated using Amicon®Ultra 4 mL centrifugal filters (Merck). After the addition of 10% glycerol ...
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bioRxiv - Biophysics 2023Quote: ... the protein was previously filtered with 0.22 µM Millex Syringe filters (Durapore PVDF, Merck KGaA, Germany) in order to exclude the presence of potential protein oligomers or aggregates ...