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Citations for Merck :
751 - 800 of 1299 citations for Human Phosphatidic Acid Phosphatase Type 2A PPAP2A ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... overnight cultures were grown at 30 °C at 200 rpm in Yeast Nitrogen Base without amino acids (Merck) prepared according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were precipitated with 50% (w/v) trichloroacetic acid (Roth) and washed once with ice-cold acetone (Merck). Protein pellets were resuspended in 1 x sodium dodecyl sulphate (SDS ...
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bioRxiv - Developmental Biology 2023Quote: ... prior to fixation embryonic zona pellucidae were removed in prewarmed (37°C) drops of Acid Tyrode’s solution (Merck) diluted in M2 ...
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bioRxiv - Immunology 2023Quote: ... Tissue samples (200 mg) were digested in 4 ml of EMSURE ISO nitric acid 65% solution (Merck, #1.00456.2508) using MARS 6 microwave extraction system ...
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bioRxiv - Genetics 2024Quote: ... 200 ng was loaded onto Evosep tips according to manufacturer’s instructions and separated using the pre-defined 30 samples per day protocol in combination with an Evosep 150 mm x 0.15mm C18 reversed phase column (EV1106 endurance column packed with 1.9 µm C18AQ particles from Dr Maisch) using 0.1% formic acid (Merck) in ultra-pure water (Biosolve ...
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bioRxiv - Cancer Biology 2024Quote: ... The final concentrations of fatty acids used in the HFHS medium were: 150 µM palmitate (Merck cat# P5585), 150 µM stearic acid (Thermo Scientific cat# A12244.06) ...
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bioRxiv - Biochemistry 2024Quote: ... Phospho peptides were then reconstituted in 10 μl of MS loading buffer containing 0.3% Trifluoroactetic acid (TFA)(Merck) and 2% Acetonitrile (ACN ...
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bioRxiv - Biophysics 2024Quote: ... Modified amino acids Fmoc-Tyr(SO2ONp)-OH and Fmoc-Tyr(PO(OBzl)OH)-OH were purchased from Merck Life Science ...
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bioRxiv - Molecular Biology 2024Quote: ... air-dried at RT for 30 min and fixed in Methanol:Acetic acid in a 3:1 ratio (Merck) at 4°C overnight ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... including protease and phosphatase inhibitor cocktails (cOmplete™, Mini Protease Inhibitor Cocktail # 11836153001, cOmplete™, Mini Protease Inhibitor Cocktail # 11836153001, Sigma Aldrich -- Merck Life Science) using a sonicator at amplitude 3-5 Hz ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... we mixed 500 mL tap water with 38 g gelatin from porcine skin (gel strength 300, Type A, G2500, Sigma-Aldrich, Merck KGaA, Darmstadt, Germany), 25 g honey (Gut & Günstig Bienenhonig flüssig ...
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bioRxiv - Biochemistry 2022Quote: ... 100 μL of SYBR® Green I solution was added to each well of a 96-well black microplate (Corning® Product number CLS 3601, polystyrene flat-bottom type, Merck KGaA, Darmstadt, Germany). The plate was mixed using a MixMate® Eppendorf machine (Eppendorf SE ...
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bioRxiv - Bioengineering 2021Quote: ... Part A consisted of 20% hPL (v/v) and 4mg/mL human fibrinogen (Merck, USA) in DMEM/F12 (Life Technologies ...
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bioRxiv - Cell Biology 2019Quote: ... They were coated for 1 hour with 50 μg/mL human plasma fibronectin (Merck Millipore) diluted in PBS and washed three times with PBS.
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bioRxiv - Molecular Biology 2021Quote: ... and goat anti-Human IgG (Fc specific)-Cy3 (1:1000 dilution in binding buffer; Merck). Finally ...
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bioRxiv - Immunology 2021Quote: ... MIP-1β by MILLIPLEX MAP Human High Sensitivity T Cell Panel (Merck Cat# HSTCMAG-28SK) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: ... Two hundred microliters of medium containing 2 μM human insulin (Merck, Sigma-Aldrich Cat# I9278) or medium only was added to the thoraxes for 10 min (the final concentration of insulin was 1 μM) ...
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bioRxiv - Cell Biology 2024Quote: ... HEK293T cells (ATCC) and FibroGRO Xeno-Free human foreskin fibroblasts (Merck, gift from Martin Lowe) were maintained in Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Cancer Biology 2023Quote: ... All human cell lines were cultured at 37℃ in 5% CO2 in DMEM media (Merck) containing 1% antibiotic (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... Primers for human H19 were used as a positive control for CTCF binding (Merck Millipore).
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bioRxiv - Immunology 2023Quote: ... cells were cultured in RPMI 1640 (ccpro) supplemented with 10 % heat-inactivated human serum (Merck), 50 U/ml IL-2 (Miltenyi) ...
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was blotted with the following antibodies: anti-human PSEN1-CTF (MAB5643; Merck Millipore); anti-PEN2 (D6G8 ...
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bioRxiv - Developmental Biology 2020Quote: ... and 0.1% sodium dodecyl sulfate (SDS; AppliChem; Darmstadt, Germany) with an ethylenediaminetetraacetic acid (EDTA)-free protease inhibitor cocktail (Merck), phosphatase inhibitor cocktail sets II (Merck ...
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bioRxiv - Molecular Biology 2020Quote: ... EmbryoMax KSOM medium with 1/2 amino acids without BSA (Reagent setup; Merck Millipore, cat. no. MR-107-D), BSA powder (Sigma-Aldrich ...
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bioRxiv - Microbiology 2019Quote: ... IFNβ production was stimulated by transfecting cells with (10 μg/mL) polyinosinic: polycytidylic acid (poly I:C) (Sigma Aldrich, Merck). Cells were lysed 6 hours after poly I:C transfection using 100μL 1X passive lysis buffer (Promega) ...
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bioRxiv - Bioengineering 2022Quote: ... that was recently established by a disruption of both AAH1 and APT1.18 Yeast strains were grown overnight at 30 °C in a synthetic defined SD medium (6.7g/L Yeast Nitrogen Base without Amino Acids (Difco) and 20g/L glucose (Merck)) containing a specific mixture of amino acids and nucleobases ...
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bioRxiv - Cell Biology 2022Quote: ... resolubilized in 25 µL of 0.05% trifluoroacetic acid in 98:2 H2O/acetonitrile (v/v) and filtrated through a 0.2 µm spin filter (Merck Millipore Ultrafree®-MC ...
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bioRxiv - Immunology 2021Quote: ... supplemented with 2 % (vol/ vol) FCS and and 10 mM 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES, Merck) and cut into 1 cm pieces ...
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bioRxiv - Bioengineering 2021Quote: ... The chemical reagents of L-cysteine (analytical reagent) and lactic acid (analytical reagent) were purchased from Merck (Darmstadt, Germany) and Fischer scientific (Merelbeke ...
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bioRxiv - Biochemistry 2019Quote: ... Samples were washed three times with 1X PBS and IgG was eluted using 0.1M formic acid (Merck, Darmstadt, Germany) followed by immediate neutralisation with 1M ammonium bicarbonate (Acros Organics ...
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bioRxiv - Biochemistry 2020Quote: ... ammonia (25% in H2O), acetic acid (≥ 98.5%), ethanol (≥ 99.5%), and sodium hydrogencarbonate (NaHCO3, ≥ 99.5%) were purchased from Merck (Darmstadt, Germany). Tris(hydroxymethyl)amino-methane (TRIS ...
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bioRxiv - Microbiology 2020Quote: ... The LC flow rate was 300 nL/min and the 100-min linear gradient ranged from 95% solvent A (H2O with 0.1% formic acid (Merck)) to 40% solvent B (100% acetonitrile with 0.1% formic acid) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... for 10 minutes at room temperature, Ponceau (C.I.14700, FUJIFILM-Wako Pure Chemical Corp., Osaka, Japan) acid fuchsin (C.I.42685, Merck-Millipore) azofloxine (C.I.18050 ...
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bioRxiv - Neuroscience 2024Quote: ... the brain sections were thawed in a desiccator and spray-coated with 80 µl of a freshly prepared solution of 2,5-dihydroxybenzoic acid (DHB, Merck) using a SMALDIPrep ultrafine pneumatic spraying system (TransMIT GmbH) ...
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bioRxiv - Molecular Biology 2023Quote: ... pH 8.0) and then transferred to 1.5 mL tubes prewarmed at 65°C acid-washed glass beads (G8772-100 Merck) suspended in 25 µL of 10% filtered SDS and 300 µL of acid phenol chloroform pH 4.5 ...
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bioRxiv - Biochemistry 2023Quote: ... was incubated in 5 mM Tris-HCl buffer (pH 7.5) containing each substrate (1% glucomannan, Neogen, MI, USA; 1% polygalacturonic acid, Neogen; 1% carboxymethyl cellulose, Merck; 1% soluble starch ...
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bioRxiv - Cancer Biology 2024Quote: ... Plasmax supplemented with 1% v/v of the 10% fatty acid-free BSA solution in PBS (Merck cat# A1595) supplemented with 10% Ethanol (Fisher Scientific cat# 10680993 ...
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bioRxiv - Microbiology 2024Quote: ... Supernatant aliquots were kept at -18°C before being diluted with 2.5 mM sulfuric acid (H2SO4, Merck, Darmstadt, Germany) at 1:6 ...
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bioRxiv - Immunology 2021Quote: ... The MILLIPLEX®MAP Human Isotyping Magnetic Bead Panel-Isotyping Multiplex Assay (HGAMMAG-301K-06, Merck) was used to measure IgG1 ...
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bioRxiv - Immunology 2019Quote: ... The MILLIPLEX®MAP Human Isotyping Magnetic Bead Panel-Isotyping Multiplex Assay (HGAMMAG-301K-06, Merck Millipore Corporation ...
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bioRxiv - Cell Biology 2019Quote: ... Complement components in the cellular supernatant were quantified using the MILLIPLEX MAP Human Complement Panel (Merck). The read out of the multiplex assay was performed in a Magpix instrument (Luminex).
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bioRxiv - Genetics 2020Quote: ... which was performed by intramuscular injection with rhFSH (recombinant human follitropin alpha, GONAL-F, Merck Serono) for 8 days ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Human Caco-2 colorectal adenocarcinoma cells (1.7×105 cells/cm2) were cultured in DMEM medium (Merck) containing 10% FCS at 37°C and 5% CO2 ...
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bioRxiv - Cell Biology 2023Quote: ... 1 mg/l hydrocortisone and 1 µg/l and human epidermal growth factor (hEGF) (both Merck). To determine EC sex ...
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bioRxiv - Microbiology 2023Quote: ... and mouse anti-human TMPRSS2 (monoclonal, clone P5H9-A3, cat num: MABF2158, 1:500, MERCK, USA) for a minimum of 2 h at RT ...
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bioRxiv - Bioengineering 2023Quote: ... the chips were coated with fibronectin (Human Plasma Fibronectin Purified Protein, Merck, Schiphol-Rijk, The Netherlands) in sterile PBS (100 μg/mL ...
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bioRxiv - Neuroscience 2024Quote: The inhibitory activity of novel compounds against human recombinant AChE (hAChE, E.C. 3.1.1.7, purchased from Merck) and human plasmatic butyrylcholinesterase (hBChE ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... cells were dissociated by mincing and enzymatic digestion of the extracellular matrix using 500 µg/ml collagenase type 1a (Merck Life Sciences UK Ltd, Gillingham, U.K) and 100 µg/ml DNAse I (Lorne Laboratories Ltd ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Flash chromatography was performed on a Varian 971-FP flash purification system using silica gel cartridges (Varian, particle size 50 μm) or on glass columns using silica gel type 60 (Merck, particle size 230 μm, 400 mesh). All compounds were obtained as oils ...
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bioRxiv - Cell Biology 2024Quote: ... and EnSCs were dissociated from endometrial biopsies by enzymatic digestion of the extracellular matrix using 500 µg/mL collagenase type Ia (Merck Life Sciences UK Ltd, Gillingham, UK) and 100 µg/mL DNase I (Lorne Laboratories Ltd ...