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Citations for Merck :
701 - 750 of 2302 citations for Acyl protein thioesterase 2 LYPLA2 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... The proteins were transferred to a PVDF membrane (Immobilon-P, Merck), blocked with 5% skim milk ...
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bioRxiv - Molecular Biology 2021Quote: ... protein concentration was quantified using a Direct Detect Infrared Spectrometer (Merck). 10 to 20 μg total protein was separated on a NuPAGE 4-12% Bis-Tris denaturing gel (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2020Quote: ... Protein extraction and Western blot using anti-puromycin antibody (Merck Millipore) was performed as described before.
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bioRxiv - Biochemistry 2021Quote: ... The proteins were then transferred to a PVDF membrane (Merck Millipore) using a semi-dry western blot transfer system set to a constant current of 200 mA for 30 min ...
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bioRxiv - Biochemistry 2020Quote: ... Selected fractions containing homogeneous protein were concentrated using Amicon filters (Merck) in respective buffers (as described above ...
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bioRxiv - Cell Biology 2021Quote: ... rabbit polyclonal Prosurfactant Protein C (1:200 pH9, AB3786, Merck Millipore), mouse monoclonal TTF-1 (clone SPT24 ...
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bioRxiv - Molecular Biology 2021Quote: Protein concentration was measured using a Direct Detect Infrared Spectrometer (Merck). 20µg of proteins were separated on a NuPAGE 4–12% Bis-Tris gel (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2020Quote: ... Proteins were visualized using Coomassie Brilliant Blue R 250 (Merck, Germany) or using the SilverQuest silver staining kit (Invitrogen Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... Total protein concentration was determined using Direct Detect Infrared Spectrometer (Merck) and a solution with 20 µg of protein was heated at 95 °C for 5 min ...
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bioRxiv - Biochemistry 2022Quote: ... the proteins were concentrated using a 10 K Amicon (Merck-Millipore), flash-frozen in liquid nitrogen and stored at −80°C until use.
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bioRxiv - Cell Biology 2022Quote: ... After the protein was transferred to a PVDF membrane (Millipore; Merck KGaA ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins were transferred to PVDF membranes (Immobilon; Merck, Darmstadt, Germany) and blocked with 5% (w/v ...
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bioRxiv - Microbiology 2022Quote: ... and protein concentration was measured by Bradford assay (Merck, Gillingham, UK).
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bioRxiv - Molecular Biology 2022Quote: ... proteins were transferred Immobilon-E PVDF membrane (Merck Millipore, Tullagreen, Ireland) and AGAL was detected by monoclonal anti-Galactosidase alpha antibody (# ab168341 ...
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bioRxiv - Biochemistry 2024Quote: ... Protein was concentrated using Amicon Ultra centrifugal filter units (Merck Millipore). Protein was then aliquoted ...
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bioRxiv - Molecular Biology 2024Quote: ... proteins were detected by using enhanced chemiluminescence (ECL) systems from Merck Millipore (Immobilon Western Chemiluminescent HRP Substrate ...
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bioRxiv - Genomics 2024Quote: ... 10µg/mL of the protein transport inhibitor Brefeldin A (Merck, B7651) was added.
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bioRxiv - Cell Biology 2023Quote: ... Proteins were electroblotted to 0.45 µm PVDF membranes (Imobilon-P, Merck Millipore ...
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bioRxiv - Cell Biology 2023Quote: ... Protein bands were detected with Immobilon Western HRP Substrate (Merck Millipore) and images captured with a CCD camera and the Bio-Rad ChemiDoc MP System (Bio-Rad) ...
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bioRxiv - Cell Biology 2023Quote: ... Subsequently proteins were precipitated on magnetic carboxylate modified beads (Merck, GE45152105050250) in the presence of 77% (vol/vol ...
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bioRxiv - Biophysics 2023Quote: The chemicals for protein expression and purification were purchased from Merck-Sigma-Aldrich and were analytically pure (grade A) ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were transferred to polyvinylidene difluoride membranes (PVDF; Merck Millipore Ltd.) at 35 V overnight in a cold room at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... proteins were extracted in n-dodecyl-β-D-maltoside (DDM, Merck) 1% detergent ...
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bioRxiv - Neuroscience 2023Quote: Biotinylated surface proteins were isolated using streptavidin-coated agarose beads (Merck). The beads were washed 2 times in lysis buffer by centrifugation at low speed (<1.5k RCF) ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were transferred to a methanol pre-activated membrane (Merck, IPFL00010) and then blocked with 5% BSA (ThermoFisher ...
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bioRxiv - Cell Biology 2023Quote: ... the proteins were transferred to a polyvinylidene fluoride membrane (Merck Millipore), followed by blocking in 2% BSA containing 50 mM Tris-HCl ...
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bioRxiv - Immunology 2022Quote: ... the fractions containing the protein were concentrated using Amicon concentrators (Merck), the protein concentration determined by measuring Abs280nm and purity assessed by visualization on SDS-PAGE gel ...
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bioRxiv - Physiology 2022Quote: ... proteins were transferred to PVDF membranes (Merck Millipore, IEVH00005, Tokyo, Japan), blocked with 2.5% non-fat milk in PBS containing 0.1% Tween 20 for 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... We transferred proteins onto Immobilion IF PDVF membrane (Merck Life Sciences) with NuPAGE transfer buffer (Thermo Fisher Scientific ...
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Critical contribution of mitochondria in the development of cardiomyopathy linked to desmin mutationbioRxiv - Pathology 2023Quote: ... Soluble proteins were then dosed using the Bicinchoninic Acid Kit (Merck) and diluted in Laemmli protein sample buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein bands were visualized using a chemiluminescence HRP substrate (Merck, Germany).
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bioRxiv - Cancer Biology 2024Quote: ... and the proteins were transferred to polyvinylidene fluoride membranes (Merck, Germany) and incubated with 10% blocking buffer (Nacalai Tesque ...
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bioRxiv - Microbiology 2024Quote: ... Protein was transferred onto an Immobilon-FL PVFD transfer membrane (MERCK) using a TE77X semi-dry transfer (Hoefer ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein was transferred to Immobilon-FL PVDF membrane (Merck Millipore, IPFL00010) at 100 volts using 1x Towbin transfer buffer with 20% methanol ...
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bioRxiv - Pathology 2024Quote: ... Transfer the proteins onto a PVDF membrane (ISEQ00010, Merck Millipore, Germany). Block the membrane with 5% skim milk for 30 minutes ...
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bioRxiv - Cancer Biology 2024Quote: Total protein was harvested using RIPA lysis buffer (Merck, Burlington, MA), and their concentrations were evaluated with BCATM protein assay kit (Pierce ...
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bioRxiv - Microbiology 2024Quote: ... A rabbit pAb against MS2 capsid proteins was purchased from Merck Millipore (ABE76-I ...
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bioRxiv - Microbiology 2022Quote: ... supplied with 2 ml of staining buffer (Lou et al., 2020) composed of PBS with 2% Fetal calf serum (FBS (Merck, Kenilworth, New Jersey, USA), and fixated with 250 µlBD Cytofix™ (BD Biosciences ...
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bioRxiv - Bioengineering 2023Quote: ... The 1.5 ml resuspended RBCs were cultured in 50 ml HPS containing 2 mM CaCl2 and 2 µM calcium ionophore-4-bromo-A23187 (C7522, Sigma-Aldrich, Merck KGaA, Darmstadt, Germany) in T75 flasks in a 5% CO2 incubator at 37°C for 16 h ...
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bioRxiv - Neuroscience 2021Quote: ... HEK293Tcells were incubated with 2 µg/ml Nocodazole (Merck# M1404) for 15min prior to lysis with the same buffer containing Nocodazole and no paclitaxel.
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bioRxiv - Developmental Biology 2022Quote: ... and 4’,6-Diamidine-2’-phenylindole dihydrochloride (DAPI, #10236276001, Merck) staining ...
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bioRxiv - Biochemistry 2019Quote: ... and 2 mM TCEP) containing EDTA-free protease inhibitor (Merck), and lysed by sonication ...
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bioRxiv - Neuroscience 2020Quote: ... 2 mM MgCl2 and 25U/ml Benzonase (# 70746, Merck Millipore). Lysates were resolved on 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels (#4561096 ...
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bioRxiv - Biophysics 2021Quote: ... Blebbistatin (B0560, Merck; used at 2 μg/ml final concentration), Gefitinib (Y0001813 ...
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bioRxiv - Neuroscience 2020Quote: ... using a Millicell ERS-2 Electrical Resistance System (Merck-Millipore). To calculate transendothelial electrical resistance (TEER ...
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bioRxiv - Plant Biology 2021Quote: ... and half-strength phosphatase inhibitor cocktail 2 and 3 (Merck). Samples were clarified twice by centrifugation at 14 000 g ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-ERK1/2 (Millipore, Merck Life Science S.L.U., Portugal), rabbit anti-phosphorylated ERK1/2 (Thr202/Tyr204 Erk1 and Thr185/Tyr187 Erk2 ...
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bioRxiv - Plant Biology 2020Quote: DSF (cis-11-methyl-2-dodecenonic) was purchased from Merck and dissolved in DMSO to obtain 100 mM stock ...
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bioRxiv - Microbiology 2020Quote: ... 2-Propanol and hydrochloric acid (HCl) were purchased from Merck, India ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 mM MgCl2] followed by DNA digestion using Benzonase (Merck). Equal amounts of total protein was loaded on NuPAGE Bis-Tris (4-12% ...