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Citations for Merck :
651 - 681 of 681 citations for Crimean Congo Haemorrhagic Fever virus CCHFV glycoprotein C Gc sheep Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... followed by a 20 min centrifugation at 4°C and 4000 rpm using 10-kD Amicon®Ultra centrifuge filter (Merck, Darmstadt, Germany) to remove free (proteinun bound ...
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bioRxiv - Immunology 2022Quote: ... the cells were transferred to a single well containing a monolayer of mitomycin C (Cat no. 50-07-7, Millipore Sigma, Merck, Darmstadt, Germany)-treated OP9/ OP9-DL1 cells or were kept unaltered ...
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bioRxiv - Biophysics 2019Quote: ... reduced and alkylated protein extracts were digested sequentially with Lys-C and trypsin on Microcon-10 filters (Merck Millipore, # MRCPRT010 Ultracel YM-10). Digested samples were desalted using ZipTips according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... cortical sections were incubated overnight at 4 °C with anti-glial fibrillary acidic protein (GFAP, 1:1000, chicken IgG, Merck Millipore, Burlington, EUA), anti-Iba1 (1:200 ...
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DJ-1 (Park7) affects the gut microbiome, metabolites and development of Innate Lymphoid cells (ILCs)bioRxiv - Immunology 2019Quote: ... Membranes were then washed twice 5 min in TBST and incubated with the primary antibody over night at 4°C (human and mouse a-syn: 610786 BD Biosciences; GAPDH: #5174, Cell Signaling and GFAP #MAB360, Merck Millipore, Germany). After incubation with the first antibody ...
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bioRxiv - Microbiology 2019Quote: ... the suspension was boiled at 95 °C for 20 minutes using a thermomixer (Thermomixer Compact Eppendorf 5350 MERCK Chemical Pty. Ltd, South Africa), then centrifuged for 5 minutes at 7500rpm ...
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bioRxiv - Microbiology 2020Quote: ... the samples were pre-enriched overnight in buffered peptone water (Oxoid, Basingstoke, UK) at 37°C and then enriched in tetrathionate brilliant green broth (Merck KGaG, Darmstadt, Germany) at 37°C ...
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bioRxiv - Immunology 2020Quote: ... and incubated overnight at 4°C followed by incubation with primary antibody: anti-tyrosine hydroxylase (TH, 1:1000, Merck Millipore, Darmstadt, Germany, AB152). After washing with TBST ...
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bioRxiv - Microbiology 2022Quote: ... After 72 hours of incubation at 33°C 5% CO2 wells were stained with Gram’s crystal violet solution (Merck KGaA, 64271 Damstadt, Germany) plus 5% formaldehyde 40% m/v (Carlo ErbaSpA ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated o/n at 4°C with the primary antibodies (Rabbit-antiGFAP: MAB3402, Merck Millipore, 1:500; Mouse-antiNeuN: MAB377, Merck Millipore, 1:500) diluted in the blocking solution ...
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bioRxiv - Immunology 2024Quote: ... The cells were incubated for 24h at 37°C and 5% CO2 in the presence of 10-9 M of mifepristone (Merck KGaA, Darmstadt, Germany) to allow expression of the G protein ...
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bioRxiv - Molecular Biology 2023Quote: ... lysates were clarified by centrifugation at 12000g and incubated 2h at 4°C with pre-equilibrated α-FLAG M2 affinity gel beads (Merck-Sigma, A2220). Beads were washed three times with lysis buffer ...
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bioRxiv - Immunology 2023Quote: ... After 72 hours of incubation at 33°C 5% CO2 wells were stained with Gram’s crystal violet solution (Merck KGaA, 64271 Damstadt, Germany) plus 5% formaldehyde 40% m/v (Carlo ErbaSpA ...
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bioRxiv - Microbiology 2023Quote: ... These intestinal pieces were washed thoroughly with PBS and digested for 30 min at 37 °C with 0.5 mg/mL collagenase D (Merck/Sigma-Aldrich, cat#11088858001) in RPMI medium (Gibco ...
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bioRxiv - Developmental Biology 2022Quote: ... The antibody was washed three times and concentrated at 4 °C using Amicon Ultra-0.5 100 KDa centrifugal filter devices (Merck Millipore, cat. no. UFC100), which remove salts and preservatives (e.g ...
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bioRxiv - Immunology 2023Quote: ... The embryos were subsequently incubated overnight at 4 °C in solution containing Cy3-conjugated α-smooth muscle actin monoclonal antibody (1:100, C6198; Merck, Darmstadt, Germany), Alexa647-Phalloidin (1:200 ...
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bioRxiv - Biochemistry 2023Quote: ... membrane using wet transfer for 3 h at 90 V/4 °C in transfer buffer (25 mM Tris; 192 mM glycine, Merck; 20% methanol, Merck). Afterwards ...
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bioRxiv - Genetics 2023Quote: ... and neo-resistance MEFs were established from WT Crl:CD1 (ICR) or ICRneo [27] foetuses at 14 d post-coitus and inactivated using 10 μg/mL mitomycin C (#M0503; Merck KGaA, Darmstadt, Germany). MEFs were cultured in the primary medium [Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Synthetic Biology 2023Quote: ... by centrifuging the purified enzyme preparations (4,000×g, 4°C) using AmiconTM Ultra-15 centrifugal filters of 10 kDa-pore size (Merck-MilliPoreSigma, Burlington, MA, USA). The absorbance at 280 nm (A280 ...
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bioRxiv - Biochemistry 2024Quote: ... were incubated for 45 min at 4°C with 10 µL agarose beads using a RAS-GTP pull-down assay kit (RAS Activation Assay Kit, Merck Millipore, 17-218). Supernatant was recovered after centrifugation ...
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bioRxiv - Molecular Biology 2024Quote: ... then stored in sterilized polypropylene bottles (Nalgon, Cat. No. 2330) at 8° C until filtered using 0.22 µm Sterivex filters (Merck-Millipore, Cat. no. SVGP01050) in 60 mL increments using disposable syringes ...
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bioRxiv - Cell Biology 2022Quote: ... The remaining sample was incubated o/n on a rotary shaker at 4 °C with 2 μg of the appropriate antibody (IgG, 12-370 Merck; pSTAT1, 7649 Cell Signaling). The next day 25 μl Dynabeads resuspended in CDB/c were added and incubated for at least 1 h at 4 °C on a rotary shaker ...
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bioRxiv - Biophysics 2019Quote: ... Constructs of Kmpv1 and KmpvSP1 were transiently expressed as fusion proteins with GFP on the C-terminus using the liposomal transfection reagent GeneJuice® (MERCK KGaA, Darmstadt, Germany). Measurements were performed at room temperature in a bath solution containing ...
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bioRxiv - Cancer Biology 2020Quote: ... and further filtered to a final volume of 1 ml by repeated centrifugation (4000 × g, 4°C) using an Amicon Ultra-15 spin-filter unit (Merck Millipore, MWCO = 100 kDa). The sample was then loaded onto a 24 ml SEC column packed with sepharose CL-4B ...
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bioRxiv - Biochemistry 2021Quote: ... modifying on previous reported conditions for the assay of MjRibK.[2] The reaction mixture was incubated at 37°C overnight and visualized using fluoresce-based thin layer chromatography (TLC) (Merck Silica gel 60 F254) using the long wavelength (365 nm ...
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bioRxiv - Genomics 2021Quote: ... The lysate was incubated for 2 hours at 4°C with 180 µl of anti-flag agarose beads (50 % slurry, ANTI-FLAG® M2 Affinity Gel, Merck, Kenilworth, NJ, USA), prewashed 3 × with 1 ml isotonic wash buffer (IsoWB ...
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bioRxiv - Cell Biology 2022Quote: ... Bacterial strains were cultured overnight at 37 °C in Luria broth (LB) medium containing 100 μg/ml ampicillin (Merck KGaA Cat#A0166, Darmstadt, Germany) and 12.5 μg/ml tetracycline (Merck KGaA Ca#T7660) ...
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bioRxiv - Plant Biology 2023Quote: ... at room temperature for 12 h and subsequently heated to 90°C for 30 min for complete digestion before being diluted with Milli-Q water (Merck Millipore, Burlington, MA, USA). A NexION 350S inductively coupled plasma mass spectrometer (PerkinElmer ...
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bioRxiv - Genetics 2023Quote: ... MAGEA4-Dy550 (1:20, Prof. G. C. Spagnoli, University Hospital of Basel, CH, conjugated) and UTF1-Dy488 (1:20, Merck/Millipore, Cat# MAB4337, conjugated). We used the DylightTM 488 and 550 labeling kits (ThermoFisher ...
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bioRxiv - Developmental Biology 2020Quote: ... Recovered oocytes placed into pre-warmed (37°C) drops (~20 μl/ ~15 oocytes) of commercial CZB media (EmbryoMax® CZB media with Phenol Red; Merck, MR-019-D; +IBMX), overlaid with mineral oil and incubated (37°C/ 5% CO2 ...
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bioRxiv - Microbiology 2023Quote: ... and untagged 1H6 (mouse monoclonal IgG2bκ) in PBS with 0.05 % sodium azide and 50 % glycerol were purchased from Merck (stored at -20°C with light protection). We used sterile-filtered 3 % bovine serum albumin (BSA heat shock fraction ...