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Citations for Genesee Scientific :
1 - 45 of 45 citations for rno mir 32 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: Discrete small scale population studies were set up in standard fly rearing bottles (Genesee Scientific Cat. #32-130) with a design similar to those described previously (Bishop et al. ...
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bioRxiv - Developmental Biology 2023Quote: Polystyrene vials (Genesee Scientific Cat# 32-110) were half filled with water and a dense weave cellulose acetate stopper (Genesee Scientific Cat# 49-101 ...
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bioRxiv - Genomics 2021Quote: ... reactions were set up in 10 μl volumes with: 5 μl 2×Apex PCR Master Mix (Genesee Scientific ...
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bioRxiv - Neuroscience 2023Quote: Small scale odor exposure for downstream qRT-PCR analysis utilized Drosophila food vials (Catalog #32-117BF, Genesee Scientific, San Diego,CA, USA). Food vials with a cottonball (Catalog # 22-456-883 ...
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bioRxiv - Neuroscience 2019Quote: ... into 10 cm petri-dishes (Genesee Scientific #32-107). Odor was added to a 6 mm filter disc (GE Healthcare #2017-006 ...
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bioRxiv - Genetics 2019Quote: ... 8oz round bottom bottles (Cat # 32-129F) from Genesee Scientific were filled with 75 mL of fly food and after three days the cross was transferred to a second bottle ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was subjected to PCR analysis with gene-specific primers in the presence of SyGreen Blue Mix (Genesee). Relative mRNA abundance was obtained by normalization to actin and tubulin housekeeping genes.
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bioRxiv - Molecular Biology 2021Quote: ... Crosses were made in polypropylene vials (Genesee Scientific Cat. #32-120), and all flies were frozen for 48 hours before being removed from the facility ...
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bioRxiv - Microbiology 2021Quote: ... Flies were reared in Wide Drosophila Vials (Cat #: 32-114, Genesee), with Droso-Plugs® (Cat # ...
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bioRxiv - Genetics 2023Quote: ... Crosses were carried out in shatter-proof polypropylene vials (Genesee Scientific Cat. #32-120). All flies and vials were frozen for 48 hours before being removed from the facility ...
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bioRxiv - Neuroscience 2022Quote: ... Experiments were conducted on assay plates (100 × 15 mm, Genesee Scientific, Cat. No: 32-107) filled with a thin layer of 2.5% agarose containing either pure agarose (EMSCO/Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... Experiments were conducted on assay plates (100 × 15 mm, Genesee Scientific, Cat. No: 32-107) filled with a thin layer of 2.5% agarose containing either pure agarose (EMSCO/Fisher ...
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bioRxiv - Genomics 2022Quote: ... We used 2μL of the resulting DNA mixture in a PCR reaction with primers (Left: 5’-CTAGCACGGAACCCTGGAAAT -3’; Right: 5’-GCAGCGCCTAGTAATCACAGA -3’) according to ApexRedTaq (Genesee Scientific, El Cajon, CA) manufacturer instructions ...
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bioRxiv - Neuroscience 2019Quote: Drosophila species were cohabitated during dialect training in standard Drosophila bottles (Genesee catalog number 32-130) containing 50 mL standard Drosophila media ...
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bioRxiv - Genetics 2020Quote: ... Experiments were carried out in polystyrene narrow culture vials (25 x 95 mm, #32-109, Genesee Scientific). Mutant lines for validation were obtained from the Exelixis collection at Harvard Medical School (Boston ...
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bioRxiv - Microbiology 2019Quote: ... The sacculi hydrolysis assay was set up in non-treated 96-well plates (Genesee Scientific). To each well ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... a single virgin male and female fly were gently aspirated into a 35 mm diameter Petri dish (Genesee Scientific, catalog #32-103) placed on top of a 17 inch LED light pad (HUION L4S ...
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bioRxiv - Genomics 2019Quote: ... was cut out and grafted to each of 60 new vials (25 mm × 95 mm, Polystyrene Reload, cat. no. 32—109RL, Genesee Scientific, USA) of maintenance food containing 10 ml media ...
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bioRxiv - Neuroscience 2022Quote: ... muscae by first embedding eight sporulating Canton-S cadavers in a 2.3 cm-diameter disc of ∼3.5 mm thick 5AS that was transferred with 6” forceps into the bottom of an empty wide-mouth Drosophila vial (Genesee #32-118). A ruler was used to mark 1.5 cm above the top of the disc ...
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bioRxiv - Developmental Biology 2023Quote: ... 50 virgin females and 25 males of the appropriate genotypes were placed in six-ounce plastic bottles (Genesee Scientific; Cat #: 32-130) with holes punched in the side using a 20-gauge needle ...
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bioRxiv - Neuroscience 2019Quote: ... Flies expressing GAL4 in a tissue specific manner driving mCD8-GFP were placed in batches into standard vials (Genesee catalog number 32-116) of 20 females ...
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bioRxiv - Genetics 2020Quote: ... Approximately 100 females per strain were allowed to oviposit on cornmeal-molasses-yeast media for 17-20 hours in 6oz polypropylene Drosophila bottles (Genesee Scientific: 32-130) with yeast paste to encourage egg laying ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... The microcapillary tubes were then wrapped in cotton wool and plugged into the top of narrow vials (Genesee Scientific; Product Number: 32-116) containing 3-10 flies on 2% agar in water ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... newly eclosed flies were anaesthetized with CO2 and groups of 5 virgin females were transferred into narrow Drosophila vials (Genesee Scientific #:32-109) containing SY media for 5 days ...
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bioRxiv - Genetics 2021Quote: ... tubes were placed on their side along the base of a series of cardboard/plastic narrow fly vial trays (Genesee Scientific, 32-124 or 59-163B), with ∼160 tubes per tray ...
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bioRxiv - Neuroscience 2023Quote: ... The resultant cDNA was mixed with primers (Integrated DNA Technology) and SyGreen Blue Mix (Genesee Scientific, 17-507) for RT–qPCR using the CFX384 real-time PCR system (BioRad) ...
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bioRxiv - Bioengineering 2022Quote: ... RT-qPCR was performed with SYBR green (qPCRBIO SyGreen Blue Mix Separate-ROX Cat #: 17-507B, Genesee Scientific). 4 μl of diluted cDNA was used for each 20 μl reaction containing a final primer concentration of 200 nM and 10 μl of SYBR green buffer solution ...
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bioRxiv - Bioengineering 2023Quote: ... RT-qPCR was performed with SYBR green (qPCRBIO SyGreen Blue Mix Separate-ROX Cat #: 17-507B, Genesee Scientific), using 4 μl of diluted cDNA for each 20 μl reaction containing a final primer concentration of 200 nM and 10 μl of SYBR green buffer solution ...
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bioRxiv - Systems Biology 2024Quote: ... Membranes were blocked 60 minutes at RT in OneBlock™ blocking buffer (#20-314, Genesee Scientific, Morrisville, NC) followed by overnight incubation at 4°C in primary antibodies for ITGAV (1:1,000 ...
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bioRxiv - Molecular Biology 2020Quote: ... qRT-PCR was performed in triplicate using 10μl reactions (2.5μl of cDNA, 5 μM of each forward and reverse primers, and 2x SYGreen mix (Genesee #17-505B)) ...
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bioRxiv - Developmental Biology 2023Quote: ... The generated cDNA was diluted 2x and 1µL was used for PCR amplification using PCR Biosystems VeriFi Mix (Genesee Scientific, 17-308B). A touchdown-PCR method was used for amplification ...
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bioRxiv - Genetics 2024Quote: ... Each PCR reaction contained Sybr Green 2X Master Mix (Genesee Scientific), appropriate primer pairs (Figure 1A ...
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bioRxiv - Evolutionary Biology 2020Quote: ... with 12.5μl Apex PCR Master Mix (Genesee Scientific, San Diego, California, USA), nine μl pure water ...
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bioRxiv - Developmental Biology 2021Quote: ... 0.5 µL of dNTPs (17-106, PCR Biosystems, Genesee Scientific, El Cajon, CA, USA), 0.75 µL of MgCl2 (42-800B3 ...
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bioRxiv - Genomics 2022Quote: ... Each 50 μL reaction contained 5 μL of 10X PCR buffer (Genesee, SanDiego, CA), 2.5 μL dNTPs (2 mM per nucleotide) ...
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bioRxiv - Physiology 2024Quote: ... SYBR Green based real-time PCR was performed using Apex qPCR Master Mix (Genesee Scientific). PCR conditions were as follows ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Real-time Taqman-PCR was assembled in qPCRBIO Probe Blue Mix (Genesee Scientific Corporation, 17-514) or TaqMan Fast Advanced Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... Real-time Taqman-PCR was assembled in qPCRBIO Probe Blue Mix (Genesee Scientific Corporation, 17-514) with 0.5μM of each primer (Forward ...
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bioRxiv - Cell Biology 2022Quote: ... and stored at -20°C in a sealed 96-well PCR plate (Genesee Scientific, cat#24-302).
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bioRxiv - Microbiology 2023Quote: ... sgRNA PCRs were pooled and purified with a Zymo Research DNA Clean and Concentrator kit (Genesee Scientific) and resuspended in 25 μL of Ultra-Pure Water (Genesee) ...
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bioRxiv - Cell Biology 2020Quote: SYBRGreen based real-time PCR was performed using Apex qPCR GREEN Master Mix (Genesee Scientific, San Diego, CA, USA). The experiments were performed as triplicates of at least three independent experiments and are expressed as a ~fold change compared to the control ...
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bioRxiv - Cancer Biology 2023Quote: ... we performed qRT-PCR of the cDNA using qPCRBIO SyGreen Blue Mix Hi-ROX (Genesee Scientific; Cat #17-506C) and the Applied Biosystems® ViiATM 7 Real-Time PCR System with 384-well Block (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Colonies of potential recombinant mutants were screened using colony PCR with 2.0 X Taq RED Master Mix (Genesee Scientific, USA). For colony PCR ...
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bioRxiv - Developmental Biology 2021Quote: ... PCR reaction reagents included 2.5 µL of 10x Buffer (42-800B3, Apex Bioresearch Products, Genesee Scientific, El Cajon, CA, USA), 0.5 µL of dNTPs (17-106 ...
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bioRxiv - Microbiology 2023Quote: ... The PCR product was gel purified from a 1.5% Agarose TAE gel using the Zymoclean Gel Extraction Kit (Genesee Scientific). This fragment was then built together with other parts of the pUCP18-GHD vector to obtain the pNLuc10-popD plasmid ...