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Citations for Genesee Scientific :
1 - 50 of 59 citations for PCR Genotyping kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... sgRNA PCRs were pooled and purified with a Zymo Research DNA Clean and Concentrator kit (Genesee Scientific) and resuspended in 25 μL of Ultra-Pure Water (Genesee) ...
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bioRxiv - Microbiology 2023Quote: ... The PCR product was gel purified from a 1.5% Agarose TAE gel using the Zymoclean Gel Extraction Kit (Genesee Scientific). This fragment was then built together with other parts of the pUCP18-GHD vector to obtain the pNLuc10-popD plasmid ...
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bioRxiv - Developmental Biology 2023Quote: ... The generated cDNA was diluted 2x and 1µL was used for PCR amplification using PCR Biosystems VeriFi Mix (Genesee Scientific, 17-308B). A touchdown-PCR method was used for amplification ...
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bioRxiv - Genetics 2024Quote: ... Each PCR reaction contained Sybr Green 2X Master Mix (Genesee Scientific), appropriate primer pairs (Figure 1A ...
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bioRxiv - Evolutionary Biology 2020Quote: ... with 12.5μl Apex PCR Master Mix (Genesee Scientific, San Diego, California, USA), nine μl pure water ...
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bioRxiv - Developmental Biology 2021Quote: ... 0.5 µL of dNTPs (17-106, PCR Biosystems, Genesee Scientific, El Cajon, CA, USA), 0.75 µL of MgCl2 (42-800B3 ...
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bioRxiv - Genomics 2022Quote: ... Each 50 μL reaction contained 5 μL of 10X PCR buffer (Genesee, SanDiego, CA), 2.5 μL dNTPs (2 mM per nucleotide) ...
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bioRxiv - Physiology 2024Quote: ... SYBR Green based real-time PCR was performed using Apex qPCR Master Mix (Genesee Scientific). PCR conditions were as follows ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Real-time Taqman-PCR was assembled in qPCRBIO Probe Blue Mix (Genesee Scientific Corporation, 17-514) or TaqMan Fast Advanced Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... Real-time Taqman-PCR was assembled in qPCRBIO Probe Blue Mix (Genesee Scientific Corporation, 17-514) with 0.5μM of each primer (Forward ...
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bioRxiv - Cell Biology 2022Quote: ... and stored at -20°C in a sealed 96-well PCR plate (Genesee Scientific, cat#24-302).
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bioRxiv - Genomics 2021Quote: ... reactions were set up in 10 μl volumes with: 5 μl 2×Apex PCR Master Mix (Genesee Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... Zymo Research Kit (Genesee: 11-328). RNA concentration was measured using the Nanodrop1000 spectrophotometer (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was subjected to PCR analysis with gene-specific primers in the presence of SyGreen Blue Mix (Genesee). Relative mRNA abundance was obtained by normalization to actin and tubulin housekeeping genes.
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bioRxiv - Neuroscience 2021Quote: ... Direct-zol™ RNA MiniPrep Kit (Genesee Scientific/Zymo Research ...
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bioRxiv - Cell Biology 2020Quote: SYBRGreen based real-time PCR was performed using Apex qPCR GREEN Master Mix (Genesee Scientific, San Diego, CA, USA). The experiments were performed as triplicates of at least three independent experiments and are expressed as a ~fold change compared to the control ...
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bioRxiv - Cancer Biology 2023Quote: ... we performed qRT-PCR of the cDNA using qPCRBIO SyGreen Blue Mix Hi-ROX (Genesee Scientific; Cat #17-506C) and the Applied Biosystems® ViiATM 7 Real-Time PCR System with 384-well Block (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Colonies of potential recombinant mutants were screened using colony PCR with 2.0 X Taq RED Master Mix (Genesee Scientific, USA). For colony PCR ...
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bioRxiv - Developmental Biology 2021Quote: ... PCR reaction reagents included 2.5 µL of 10x Buffer (42-800B3, Apex Bioresearch Products, Genesee Scientific, El Cajon, CA, USA), 0.5 µL of dNTPs (17-106 ...
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bioRxiv - Developmental Biology 2019Quote: ... or Direct-zol RNA Miniprep Kit (Genesee Scientific), and RNA was reverse-transcribed by Superscript IV (Invitrogen ...
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bioRxiv - Cancer Biology 2022Quote: ... and purified using Zymo ChIP DNA Concentrator kit (Genesee Scientific #11-379C ...
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bioRxiv - Neuroscience 2023Quote: Small scale odor exposure for downstream qRT-PCR analysis utilized Drosophila food vials (Catalog #32-117BF, Genesee Scientific, San Diego,CA, USA). Food vials with a cottonball (Catalog # 22-456-883 ...
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bioRxiv - Microbiology 2021Quote: ... RNA was isolated using the RNeasy kit (Genesee Scientific, USA) following manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... Zymo DNA clean and concentrator kit (D4004) was from Genesee Scientific ...
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bioRxiv - Genomics 2022Quote: ... We used 2μL of the resulting DNA mixture in a PCR reaction with primers (Left: 5’-CTAGCACGGAACCCTGGAAAT -3’; Right: 5’-GCAGCGCCTAGTAATCACAGA -3’) according to ApexRedTaq (Genesee Scientific, El Cajon, CA) manufacturer instructions ...
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bioRxiv - Biochemistry 2021Quote: ... Plasmid DNA was purified using Zyppy Plasmid Miniprep Kit (Genesee Scientific) and sequenced using primers as followed ...
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bioRxiv - Microbiology 2022Quote: ... and genomic DNA was extracted using the Quick-DNA Miniprep Kit (Genesee) or the DNeasy Blood & Tissue Kit (Qiagen) ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was isolated using Quick-RNA MiniPrep Kit (Genesee Scientific: 11-328). When necessary ...
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bioRxiv - Biochemistry 2019Quote: ... and gel-purified with ZymoClean Gel DNA Recovery Kit (Genesee Scientific, #11-300C) to produce the final library ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were visualized using ProSignal Pico ECL Western Blotting detection kit (Genesee Scientific).
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bioRxiv - Immunology 2020Quote: ... 8 and 17 hours by using Direct-zol RNA MiniPrep Kit (Genesee Scientific) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... and the supernatant processed by the Direct-zol RNA MiniPrep kit (Genesee Scientific) per the manufacturer’s instructions with an additional dry spin after disposing of the final wash to prevent EtOH carryover ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were then visualized using the ProSignal Pico ECL Western Blotting detection kit (Genesee Scientific).
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bioRxiv - Immunology 2023Quote: ... Total protein concentrations were determined using BCA protein assay kit (Genesee Scientific, Cat# 18-440). Proteins were separated using SDS-PAGE electrophoresis and then transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Biochemistry 2024Quote: ... the modified RNA was purified using the RNA Clean & Concentrator-5 kit (Genesee Scientific #R1014). The RNA sample was eluted from the column with 7 μL of RNase-Free water ...
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bioRxiv - Biochemistry 2024Quote: ... The DNA was then purified using the Zymoclean Gel DNA Recovery Kit (Genesee Scientific #11-301C). Lastly ...
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bioRxiv - Biochemistry 2024Quote: ... the cDNA product was purified using the Oligo Clean and Concentrator Kit (Genesee Scientific #11-380B). Before starting the purification ...
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bioRxiv - Genetics 2022Quote: Genomic DNA was isolated from yeast strains using the Zymo YeastStar genomic DNA isolation kit (Genesee Scientific) using the manufacturer’s recommended protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... gDNA was extracted using the YeaStar Genomic DNA Kit (Genesee Scientific, San Diego, CA, Cat #11-323) according to the manufacturer’s “Protocol 1” ...
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bioRxiv - Microbiology 2023Quote: ... pooled and purified with a Zymo Research DNA Clean and Concentrator kit (Genesee Scientific, San Diego, CA) and resuspended in 25 μL of ultra-pure water (Genesee).
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bioRxiv - Bioengineering 2021Quote: ... and isolated with the Zymopure II Midi Prep kit following the manufacturer’s protocol (Genesee Scientific, San Diego, CA). 4 µg plasmid was digested for 4 h at 37°C with BstXI and XhoI (New England Biolabs) ...
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bioRxiv - Cell Biology 2022Quote: ... Total RNA was extracted from the cells using Zymo Quick-RNA MiniPrep Kit (Genesee Scientific, Cat #11-328). Reverse transcription was performed using iScript RT supermix (Biorad ...
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bioRxiv - Cell Biology 2023Quote: ... Total RNA was extracted from the samples using the Zymo Quick-RNA Miniprep Kit (Genesee, Cat. #11–327) or Qiagen RNeasy Mini Kit (Qiagen ...
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bioRxiv - Genetics 2023Quote: ... At L4 RNA was extracted using Tri-reagent and Direct-zol RNA MiniPrep Kit (Zymo research, Genesee Scientific) as per kit recommendations ...
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bioRxiv - Genetics 2023Quote: ... All plasmid purification was done using overnight culture and the Zymo Zyppy Plasmid Miniprep Kit (Genesee 11-30). Plasmids were verified using Sanger sequencing (performed by Quintara) ...
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bioRxiv - Cell Biology 2020Quote: ... and total RNA was extracted from the samples using the Zymo Quick-RNA Miniprep Kit (Genesee, Cat #11-327) or Qiagen RNeasy Mini Kit (Qiagen Cat #74106) ...
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bioRxiv - Cancer Biology 2020Quote: ... total RNA was extracted from cultured cells using the Quick RNA MiniPrep Zymo Research Kit (Genesee Scientific, #11-328) or the RNeasy Mini Kit (Qiagen ...
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bioRxiv - Biochemistry 2024Quote: ... and it was included in the RNA Clean and Concentrator-5 with the DNase I kit (Genesee Scientific #R1014). The RNA was then purified using the same kit ...
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bioRxiv - Microbiology 2021Quote: RNA was isolated from washed tissue samples using Zymo Direct-zol RNA MicroPrep kits according to the manufacturer’s instructions (11-330MB; Genesee Scientific). RNA concentration was quantified using a Nanodrop spectrophotometer (ThermoFisher) ...
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bioRxiv - Biochemistry 2023Quote: ... The rest of the RNA purification was performed using the Direct-zol RNA MiniPrep Kit (Genesee Scientific, catalog #11-330) and Zymo-Spin IIICG columns (Zymo Research ...