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Citations for Biotek :
1 - 50 of 77 citations for Precast Protein Gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... Approximately 20 ug of protein from each sample was resolved on a 4-20% gradient gel (Biotek), then visualized by SimplyBlue SafeStain.
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bioRxiv - Synthetic Biology 2024Quote: ... the proper band was isolated using gel extraction on a 0.5% agarose gel followed by purification with the EZNA gel extraction kit (Omega BioTek). The concentration of the DNA was measured using AccuClear (Biotium ...
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bioRxiv - Microbiology 2022Quote: ... gel extracted (E.Z.N.A.® Gel Extraction Kit, Omega Biotek, Norcross, GA, United States) and sent to an external lab for Sanger sequencing (Genewiz from Azenta Life Science ...
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bioRxiv - Developmental Biology 2022Quote: ... purified (EZNA Gel Extraction Kit, Omega Biotek), quantified (NanoDrop Technologies Inc. ...
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bioRxiv - Developmental Biology 2022Quote: ... PCR products were purified by agarose gel and extracted using a GFX gel band extraction kit according to the manufacturer’s instruction (Omega Biotek, USA). 500ng of purified PCR fragments were used to prepare Digoxigenin or Fluorescein labelled antisense probes ...
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bioRxiv - Microbiology 2019Quote: ... agarose gel electrophoresis and Microplate reader (BioTek, FLx800). Negative control with only reagent was added to evaluate laboratory microbial contaminations in the agarose gel electrophoresis ...
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bioRxiv - Synthetic Biology 2023Quote: ... and DNA purification and gel extraction kits (Omega Biotek, GA, USA) were used to amplify DNA fragments and purify the PCR products ...
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bioRxiv - Cancer Biology 2023Quote: ... Supernatant protein concentrations were quantified using a BCA Protein Assay kit (Pierce) and a spectrophotometric plate reader (BioTek). Most protein lysates were separated by running 30-100 μg of lysate on 10% Bis-Tris NuPage gels (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... and protein concentrations determined using a Bicinchoninic Acid Assay (Pierce BCA Protein Assay, ThermoScientific) on an Eon spectrophotometer (BioTek). Total protein lysates were separated via SDS-PAGE and normalized across the isolates using Stain-FreeTM (BioRad ...
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bioRxiv - Synthetic Biology 2021Quote: ... The approximately 8100bp amplicon was purified using a gel extraction kit (Omega Biotek). DNA concentration of both linear fragments was measured using the Take3 plate coupled with a Biotek Cytation 5 plate reader ...
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bioRxiv - Molecular Biology 2020Quote: ... and PCR products were purified using the EZNA Gel Extraction kit (Omega BioTek).
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bioRxiv - Bioengineering 2023Quote: ... and purified by DNA purification and gel extraction kits (Omega Biotek, GA, USA). For the ligation-dependent cloning ...
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bioRxiv - Synthetic Biology 2022Quote: ... and then purified by DNA purification and gel extraction kits (Omega BioTek, GA, USA). For the ligation-dependent cloning ...
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bioRxiv - Molecular Biology 2022Quote: ... and dsDNA fragments are extracted and purified from the gel (Omega Biotek, D2500-02). The purified DNA fragments are quantified by using a Nanodrop 2000 spectrophotometer.
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bioRxiv - Genomics 2020Quote: ... SQ RNA/DNA/Protein Extraction Kit (Omega Biotek Inc.). Briefly ...
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bioRxiv - Molecular Biology 2020Quote: ... and mono-nucleosome bands were identified and selected under blue light and gel purified (Omega Biotek). Mononucleosomal DNA fragments were sequenced on an Illumina HiSeq 2500 instrument (2×125 paired-end sequencing) ...
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bioRxiv - Cell Biology 2020Quote: Total protein levels were calibrated using Bicinchoninic Acid (BCA) assay (ThermoFisher Pierce BCA Protein Assay kit, OD measured with BioTek synergy 2 multi-mode multiplate reader). Samples were ran on a 9% SDS polyacrylamide gel ...
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bioRxiv - Evolutionary Biology 2022Quote: ... then validated on agarose gel and concentration was measured with Epoch Microplate Spectrophotometer (BioTek Instruments, Inc., USA). Next ...
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bioRxiv - Bioengineering 2020Quote: ... Extracted RNA was quality-checked using agarose gel electrophoresis and Synergy HTX Multi-Mode Reader (BioTek, USA). Samples were selected for metatranscriptomic sequencing from each phase of operation (anaerobic ...
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bioRxiv - Immunology 2020Quote: ... Samples were analyzed by agarose gel electrophoresis and purified PCR products (ENZA cycle pure kit; Omega Biotek) were cloned into the pCR2.1 vector using the Original TA cloning kit (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... The −500 to +216 region corresponding to the HSPA1B promoter was amplified from the plasmid by PCR with Phusion polymerase (Thermo-Fisher #F530S) and purified via E.Z.N.A Gel Extraction Kit (Omega BioTek #D2500). The DNA was ethanol precipitated ...
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bioRxiv - Physiology 2023Quote: ... Protein concentration in the host fraction was measured on a spectrophotometer (BioTek PowerWave XS2 ...
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bioRxiv - Synthetic Biology 2021Quote: ... Both fragments were cleaned with the Viogene Gel/PCR DNA Isolation Kit (Viogene-Biotek Corporation, New Taipei, Taiwan R.O.C.) and then fused in an overlapping PCR to make an IS1 fragment lacking inverted repeats but having SspI sites on the ends and an XhoI site in the center ...
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bioRxiv - Plant Biology 2022Quote: ... Protein concentration was quantified using a Synergy H1 microplate reader (BioTek Instruments, Inc.) with a Take3 Micro-Volume Plate (BioTek Instruments ...
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bioRxiv - Biochemistry 2020Quote: ... 1 μg of total RNA (purity/integrity assessed by agarose gel electrophoresis and concentration determined by A260nm using a BioTek Cytation3 microplate reader with a Take3 accessory ...
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bioRxiv - Microbiology 2020Quote: ... The DNA concentration and integrity were determined by electrophoresis on 1% agarose gels containing ethidium bromide and spectrophotometrically using an EPOCH instrument (BioTek). After confirmation ...
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bioRxiv - Biochemistry 2019Quote: ... 1 μg of total RNA (purity/integrity assessed by agarose gel electrophoresis and concentration determined by A260nm using a BioTek Cytation3 microplate reader with a Take3 accessory ...
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bioRxiv - Genetics 2021Quote: ... The quality of the DNA was determined by agarose gel electrophoresis and by determining the OD260:OD280 ratio using a Nanodrop spectrophotometer (Epoch 2, Agilent BioTek). De novo genome sequencing was carried out by Novogene (www.novogene.com ...
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bioRxiv - Immunology 2022Quote: ... and lambda chains.48 The products from the second PCR were analyzed by agarose gel electrophoresis and purified PCR products (ENZA cycle pure kit; Omega Biotek) were submitted to Genewiz for sequencing ...
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bioRxiv - Evolutionary Biology 2019Quote: ... SOD was expressed as units/mg/ml protein (quantified using a Biotek Take3 protocol). Red blood cells were lysed as per the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by the addition of protein in Alpha Buffer using the EL460 dispenser (BioTek). After 15 min of incubation ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... DNA concentration and purity was monitored by electrophoresis on 2% (w/v) agarose gels and spectrophotometrically by determination of A260/A280 ratios (Synergy HT Microplate Reader, BioTek, USA). Amplicon based metagenomics was performed using the NEBNext® Ultra™ IIDNA Library Prep Kit (Cat No ...
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bioRxiv - Physiology 2022Quote: ... protein concentration was determined by absorbance at 450 nm in a plate reader (Biotek Instrument).
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bioRxiv - Immunology 2020Quote: ... Protein quantification was carried out after thawing in a Synergy HTX Multi-Mode Reader (Biotek).
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bioRxiv - Plant Biology 2022Quote: ... with a BSA range and protein concentration was quantified with a ELx808 microplate reader (Biotek). Enzymatic activity was measured via the derived products generated from a 4-MUG substrate (M1404 ...
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bioRxiv - Physiology 2019Quote: ... and an aliquot of the synthesized RNA probes were then visually assessed using standard agarose gel electrophoresis and quantified on a SYNERGY 2 Microplate reader (Biotek, Winooski, VT).
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bioRxiv - Bioengineering 2021Quote: ... after which the gels were transferred to a black 96-well plate and fluorescence readings were taken (BioTek Synergy H1 microplate reader).
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bioRxiv - Synthetic Biology 2021Quote: ... Both products were DpnI digested and cleaned with the Viogene Gel/PCR DNA Isolation Kit (Viogene-Biotek Corporation, New Taipei, Taiwan R.O.C.). These fragments were assembled in a Circular Polymerase Extension Cloning (CPEC ...
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bioRxiv - Microbiology 2020Quote: ... Isolated gDNA was checked for quality and concentration by gel electrophoresis and by spectrophotometry using a Take3 Micro-Volume Plate (BioTek, Winooski, VT, USA). Approximately 100 ng of gDNA was used as template for PCR using Q5 Hot Start DNA Polymerase (New England Biolabs (NEB) ...
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bioRxiv - Plant Biology 2022Quote: ... The DNA resulting from this extraction was assessed for quality in a 1% agarose gel and quantified using Synergy™ HT Multi-detection microplate reader (BioTek®, USA). RAD-tag libraries were developed by the Beijing Genomic Institute (BGI ...
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bioRxiv - Immunology 2021Quote: ... Protein content was measured using 260/280 absorbance ratio with Take3 Micro-Volume Plate reader (BioTek)
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bioRxiv - Plant Biology 2022Quote: ... Protein concentration was estimated using a Synergy H1 microplate reader (BioTek Instruments, Inc., Winooski, VT, USA) with a Take3 Micro-Volume Plate (BioTek Instruments ...
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bioRxiv - Biochemistry 2021Quote: ... The absorbance of the concentrated protein was taken at 280 nm using Synergy H4 Hybrid Reader (BioTek) on a Take3 plate ...
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bioRxiv - Immunology 2021Quote: ... Concentrations of antigens were determined using standard curves constructed with purified recombinant proteins and calculated with Gen5 3.02.2 (BioTek).
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bioRxiv - Cell Biology 2022Quote: ... 0.5 mM DTT) into the protein mix using the automated dispenser of a Synergy 4 microplate reader (BioTek) to a final volume of 200 μl and a final concentration of 2 μM actin in 1x KMEI with 0.05% antifoam ...
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bioRxiv - Bioengineering 2020Quote: ... Reactions for the fluorescent proteins were carried out in a Synergy Neo2 HTS Multi-Mode Microplate Reader (BioTek) at 282 cpm.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Protein quantification of exosome and HCQ@ZnS@exo@iRGD were done by using Bradford assay (BioTek, Winooski, USA). The stability of the vesicles was studied by monitoring the change in size and PDI over a 7-day period.
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bioRxiv - Immunology 2020Quote: ... unspecific protein binding sites were blocked by incubating sections with PBS containing 10% normal donkey serum (Pan-Biotek), 2% BSA and 0.2% Triton-X100 for 1 hour ...
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bioRxiv - Biophysics 2022Quote: In vivo and in vitro spectra of all proteins were recorded using Synergy H4 Hybrid Microplate Reader (BioTek) with resolution of 9 nm ...
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bioRxiv - Molecular Biology 2023Quote: ... and the total protein in each extract was estimated by reading the OD280nm using the spectrophotometer (Epoch, BioTek). For the Western blot ...