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Citations for Biotek :
1 - 50 of 70 citations for Nucleic Acid Electrophoresis Gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... agarose gel electrophoresis and Microplate reader (BioTek, FLx800). Negative control with only reagent was added to evaluate laboratory microbial contaminations in the agarose gel electrophoresis ...
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bioRxiv - Plant Biology 2020Quote: ... Nucleic acid concentration was measured using a microplate reader Synergy HT (Biotek, Germany), with the software Gen5™ (Biotek ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... with the Nucleic Acid Quantification workflow on a Synergy HT plate reader (BioTek) with Gen5 software.
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... and yields were quantified the Nucleic Acid Quantification workflow on a Synergy HT plate reader (BioTek) with Gen5 software ...
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bioRxiv - Microbiology 2020Quote: ... and RNA concentration was measured by nucleic acid quantification in Epoch Microplate Spectrophotometer (BioTek, Winooski, VT, USA). Transfer RNAs and 5S RNA were then removed using the MEGAclear Kit Purification for Large Scale Transcription Reactions (AMBION ...
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bioRxiv - Immunology 2020Quote: ... NET formation was stimulated with 100nM PMA and detected with SYTOX™ Green nucleic acid stain (Thermo) at 528nm during 6 hr incubation at 37°c in a Synergy HT (BioTek) plate reader (as previously described).[31] NET formation was confirmed by fluorescence microscopy.
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bioRxiv - Bioengineering 2020Quote: ... Extracted RNA was quality-checked using agarose gel electrophoresis and Synergy HTX Multi-Mode Reader (BioTek, USA). Samples were selected for metatranscriptomic sequencing from each phase of operation (anaerobic ...
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bioRxiv - Immunology 2020Quote: ... Samples were analyzed by agarose gel electrophoresis and purified PCR products (ENZA cycle pure kit; Omega Biotek) were cloned into the pCR2.1 vector using the Original TA cloning kit (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... 1 μg of total RNA (purity/integrity assessed by agarose gel electrophoresis and concentration determined by A260nm using a BioTek Cytation3 microplate reader with a Take3 accessory ...
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bioRxiv - Microbiology 2020Quote: ... The DNA concentration and integrity were determined by electrophoresis on 1% agarose gels containing ethidium bromide and spectrophotometrically using an EPOCH instrument (BioTek). After confirmation ...
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bioRxiv - Biochemistry 2019Quote: ... 1 μg of total RNA (purity/integrity assessed by agarose gel electrophoresis and concentration determined by A260nm using a BioTek Cytation3 microplate reader with a Take3 accessory ...
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bioRxiv - Genetics 2021Quote: ... The quality of the DNA was determined by agarose gel electrophoresis and by determining the OD260:OD280 ratio using a Nanodrop spectrophotometer (Epoch 2, Agilent BioTek). De novo genome sequencing was carried out by Novogene (www.novogene.com ...
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bioRxiv - Immunology 2022Quote: ... and lambda chains.48 The products from the second PCR were analyzed by agarose gel electrophoresis and purified PCR products (ENZA cycle pure kit; Omega Biotek) were submitted to Genewiz for sequencing ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... DNA concentration and purity was monitored by electrophoresis on 2% (w/v) agarose gels and spectrophotometrically by determination of A260/A280 ratios (Synergy HT Microplate Reader, BioTek, USA). Amplicon based metagenomics was performed using the NEBNext® Ultra™ IIDNA Library Prep Kit (Cat No ...
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bioRxiv - Physiology 2019Quote: ... and an aliquot of the synthesized RNA probes were then visually assessed using standard agarose gel electrophoresis and quantified on a SYNERGY 2 Microplate reader (Biotek, Winooski, VT).
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bioRxiv - Microbiology 2020Quote: ... Isolated gDNA was checked for quality and concentration by gel electrophoresis and by spectrophotometry using a Take3 Micro-Volume Plate (BioTek, Winooski, VT, USA). Approximately 100 ng of gDNA was used as template for PCR using Q5 Hot Start DNA Polymerase (New England Biolabs (NEB) ...
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bioRxiv - Synthetic Biology 2024Quote: ... the proper band was isolated using gel extraction on a 0.5% agarose gel followed by purification with the EZNA gel extraction kit (Omega BioTek). The concentration of the DNA was measured using AccuClear (Biotium ...
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bioRxiv - Microbiology 2023Quote: ... RNA samples were visualized by RNA electrophoresis and quantified on Synergy™ HT (BioTek). mRNA was then purified using NEBNext® Poly(A ...
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bioRxiv - Microbiology 2022Quote: ... gel extracted (E.Z.N.A.® Gel Extraction Kit, Omega Biotek, Norcross, GA, United States) and sent to an external lab for Sanger sequencing (Genewiz from Azenta Life Science ...
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bioRxiv - Developmental Biology 2022Quote: ... purified (EZNA Gel Extraction Kit, Omega Biotek), quantified (NanoDrop Technologies Inc. ...
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bioRxiv - Developmental Biology 2022Quote: ... PCR products were purified by agarose gel and extracted using a GFX gel band extraction kit according to the manufacturer’s instruction (Omega Biotek, USA). 500ng of purified PCR fragments were used to prepare Digoxigenin or Fluorescein labelled antisense probes ...
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bioRxiv - Synthetic Biology 2023Quote: ... and DNA purification and gel extraction kits (Omega Biotek, GA, USA) were used to amplify DNA fragments and purify the PCR products ...
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bioRxiv - Plant Biology 2020Quote: ... The total amino acid content was then measured in a Synergy HT plate reader (BioTek, Germany) at a wavelength of 550 nm ...
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bioRxiv - Molecular Biology 2019Quote: ... 5 mM nitrilotriacetic acid) and FP determined with a Synergy 2 plate reader (BioTek, Winooski, VT) as described (Patriarchi et al. ...
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bioRxiv - Synthetic Biology 2021Quote: ... The approximately 8100bp amplicon was purified using a gel extraction kit (Omega Biotek). DNA concentration of both linear fragments was measured using the Take3 plate coupled with a Biotek Cytation 5 plate reader ...
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bioRxiv - Molecular Biology 2020Quote: ... and PCR products were purified using the EZNA Gel Extraction kit (Omega BioTek).
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bioRxiv - Bioengineering 2023Quote: ... and purified by DNA purification and gel extraction kits (Omega Biotek, GA, USA). For the ligation-dependent cloning ...
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bioRxiv - Neuroscience 2024Quote: ... The extinction of the bicinchoninic acid (BCA) was measured at 562 nm utilizing the µQuant spectrometer (BioTek). A standard curve was set up using albumin to determine the protein concentration of each sample.
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bioRxiv - Synthetic Biology 2022Quote: ... and then purified by DNA purification and gel extraction kits (Omega BioTek, GA, USA). For the ligation-dependent cloning ...
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bioRxiv - Molecular Biology 2022Quote: ... and dsDNA fragments are extracted and purified from the gel (Omega Biotek, D2500-02). The purified DNA fragments are quantified by using a Nanodrop 2000 spectrophotometer.
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bioRxiv - Cancer Biology 2021Quote: ... Dimerization of Homovanilic acid was recorded every 30 seconds for 20 minutes with a BioTek Synergy H1 platereader (BioTek) spectrophotometer with an Ex = 320 nm and Em = 420 nm.
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bioRxiv - Immunology 2020Quote: ... After 5 to 10 min the reaction was stopped with 100 µL 1M sulfuric acid and the plates were read at 450 and 570 nm with the Cytation3 Imaging Reader (Biotek). A standard curve was generated using rec 2-12C antibody (Absolute Antibody) ...
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bioRxiv - Immunology 2021Quote: ... Reactions were quenched with 1 N hydrochloric acid and absorbance was measured at 450 nm using a spectrophotometer (Biotek).
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bioRxiv - Microbiology 2023Quote: ... and protein concentrations determined using a Bicinchoninic Acid Assay (Pierce BCA Protein Assay, ThermoScientific) on an Eon spectrophotometer (BioTek). Total protein lysates were separated via SDS-PAGE and normalized across the isolates using Stain-FreeTM (BioRad ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were destained in destaining solution (40% EtOH and 10% acetic acid) and crystal violet quantitated at OD595 (Biotek). To measure cell clonogenicity ...
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bioRxiv - Molecular Biology 2020Quote: ... and mono-nucleosome bands were identified and selected under blue light and gel purified (Omega Biotek). Mononucleosomal DNA fragments were sequenced on an Illumina HiSeq 2500 instrument (2×125 paired-end sequencing) ...
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bioRxiv - Immunology 2021Quote: ... TMB substrate was added and incubated for 2-5 min and reaction was stopped with 50µl of 1M sulphuric acid and absorbance was measured at 450 and 630nm (reference wavelength) on the Cytation3 Imaging Reader (Biotek). The MN titre was defined as the final dilution of serum that caused ≥50% reduction in NP expression.
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bioRxiv - Microbiology 2019Quote: ... The reaction was stopped with 2M sulfuric acid and absorbance read at 450 nm with a Synergy H1 micro plate reader (BioTek).
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bioRxiv - Immunology 2020Quote: ... substrate the reaction was stopped with 1M sulfuric acid and absorbance was measured at 450 nm and 570 nm (reference wavelength) on the Cytation3 Imaging Reader (Biotek). The MN titers were expressed as half maximal inhibitory dilution (50% Inhibitory titer is ...
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bioRxiv - Microbiology 2021Quote: ... The reaction was stopped by adding 50µL of 3M hydrochloric acid and then absorbance (at 490nm) was immediately determined using Synergy H1 hybrid multimode microplate reader (BioTek). PRISM 7.0 was used to determine the effective concentration of each virus that would yield detectable NA activity ...
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bioRxiv - Microbiology 2022Quote: ... and sulfuric acid and quantified at 450 nm using a BioTek Synergy H1 plate reader (BioTek Instruments Inc., Winoski, VT). Values were analyzed in Prism 9.0 (GraphPad ...
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bioRxiv - Microbiology 2022Quote: ... Wells were washed twice with PBS and stained biofilms were resuspended in 7% acetic acid (v/v) and absorbance was read at 595 nm in a Synergy H1 hybrid multimode reader (BioTek).
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bioRxiv - Molecular Biology 2022Quote: ... stopping the reaction with 2M sulfuric acid and by measuring the absorbance at 450nm with a microplate spectrophotometer (Epoch BioTek).
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bioRxiv - Microbiology 2020Quote: ... the reaction was stopped with 50 μls of 3M hydrochloric acid and absorbance was measured at 490 nm (OD490) using a Synergy 4 (BioTek) plate reader ...
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bioRxiv - Microbiology 2021Quote: ... stopped using 3M hydrochloric acid (HCL) solution and plates were read at 490nm wavelength using ELISA plate reader (BioTek Instruments). The endpoint antibody titer was determined by using a cut-off value which is three-times the absorbance of first dilution of mock (uninfected ...
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bioRxiv - Immunology 2020Quote: ... After 5 to 10 min the reaction was stopped with 100 µl 1M sulfuric acid and the plates were read at 450 and 570 nm with the Cytation3 Imaging Reader (Biotek). The cut off value was defined as the average of all blank wells plus three times the standard deviation of the blank wells.
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bioRxiv - Immunology 2020Quote: ... After 5 to 10 min the reaction was stopped with 100 µl 1M sulfuric acid and the plates were read at 450 and 570 nm with the Cytation3 Imaging Reader (Biotek). The cut off value was defined as the average of all blank wells plus three times the standard deviation of the blank wells.
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bioRxiv - Microbiology 2022Quote: ... The absorbance was quantified at 570 nm using 30% acetic acid in water as the blank by a plate reader (Synergy H1 from BioTek).
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bioRxiv - Cancer Biology 2023Quote: ... Colonies were solubilized in 10% acetic acid to quantify clonogenic capacity and read for absorbance at 590nm in a plate reader (BioTek).
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bioRxiv - Biochemistry 2022Quote: ... Reactions were quenched with 2 M sulfuric acid before determining absorbance at 450 nm using a Synergy BioHTX plate reader (BioTek). The background value from wells containing no serum was averaged and subtracted from all wells on the plate.