Labshake search
Citations for Biotek :
1 - 50 of 56 citations for Crimean Congo Hemorrhagic Fever Virus Gn Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2021Quote: ... the fluorescence of congo red was measured using the plate reader (BioTek microplate spectrophotometer) with an excitation wavelength at 485nm and emission at 612 nm ...
-
bioRxiv - Genetics 2021Quote: ... We measured reporter virus transduction activity on a luminometer (BioTek) using the Renilla Glo Kit (Promega ...
-
bioRxiv - Molecular Biology 2021Quote: ... Percentages of virus-infected cells was quantified by using the Gen5 Image+ software (BioTek).
-
bioRxiv - Immunology 2021Quote: ... Virus fluorescence was measured with a Cytation Hybrid Multi-Mode reader at 488 nm (Biotek Instruments).
-
bioRxiv - Microbiology 2021Quote: ... Virus fluorescence was measured with a Cytation Hybrid Multi-Mode reader at 488 nm (Biotek Instruments).
-
bioRxiv - Microbiology 2022Quote: ... The cell plate was infected with virus at approximately 0.5 TCID50/well and incubated at 37 °C in the Cytation 5 (BioTek) supplemented with 5% CO2 ...
-
bioRxiv - Immunology 2020Quote: ... and fluorescent virus-infected foci were detected 16-24 hours after inoculation with a Cytation 7 Cell Imaging Multi-Mode Reader (BioTek) with Gen5 Image Prime version 3.09 ...
-
bioRxiv - Cancer Biology 2023Quote: ... Supernatant protein concentrations were quantified using a BCA Protein Assay kit (Pierce) and a spectrophotometric plate reader (BioTek). Most protein lysates were separated by running 30-100 μg of lysate on 10% Bis-Tris NuPage gels (Invitrogen ...
-
bioRxiv - Microbiology 2023Quote: ... and protein concentrations determined using a Bicinchoninic Acid Assay (Pierce BCA Protein Assay, ThermoScientific) on an Eon spectrophotometer (BioTek). Total protein lysates were separated via SDS-PAGE and normalized across the isolates using Stain-FreeTM (BioRad ...
-
bioRxiv - Genomics 2020Quote: ... SQ RNA/DNA/Protein Extraction Kit (Omega Biotek Inc.). Briefly ...
-
bioRxiv - Cell Biology 2020Quote: Total protein levels were calibrated using Bicinchoninic Acid (BCA) assay (ThermoFisher Pierce BCA Protein Assay kit, OD measured with BioTek synergy 2 multi-mode multiplate reader). Samples were ran on a 9% SDS polyacrylamide gel ...
-
bioRxiv - Physiology 2023Quote: ... Protein concentration in the host fraction was measured on a spectrophotometer (BioTek PowerWave XS2 ...
-
bioRxiv - Plant Biology 2022Quote: ... Protein concentration was quantified using a Synergy H1 microplate reader (BioTek Instruments, Inc.) with a Take3 Micro-Volume Plate (BioTek Instruments ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... SOD was expressed as units/mg/ml protein (quantified using a Biotek Take3 protocol). Red blood cells were lysed as per the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2024Quote: ... followed by the addition of protein in Alpha Buffer using the EL460 dispenser (BioTek). After 15 min of incubation ...
-
bioRxiv - Physiology 2022Quote: ... protein concentration was determined by absorbance at 450 nm in a plate reader (Biotek Instrument).
-
bioRxiv - Immunology 2020Quote: ... Protein quantification was carried out after thawing in a Synergy HTX Multi-Mode Reader (Biotek).
-
bioRxiv - Plant Biology 2022Quote: ... with a BSA range and protein concentration was quantified with a ELx808 microplate reader (Biotek). Enzymatic activity was measured via the derived products generated from a 4-MUG substrate (M1404 ...
-
bioRxiv - Immunology 2021Quote: ... Protein content was measured using 260/280 absorbance ratio with Take3 Micro-Volume Plate reader (BioTek)
-
bioRxiv - Plant Biology 2022Quote: ... Protein concentration was estimated using a Synergy H1 microplate reader (BioTek Instruments, Inc., Winooski, VT, USA) with a Take3 Micro-Volume Plate (BioTek Instruments ...
-
bioRxiv - Biochemistry 2021Quote: ... The absorbance of the concentrated protein was taken at 280 nm using Synergy H4 Hybrid Reader (BioTek) on a Take3 plate ...
-
bioRxiv - Bioengineering 2024Quote: ... Approximately 20 ug of protein from each sample was resolved on a 4-20% gradient gel (Biotek), then visualized by SimplyBlue SafeStain.
-
bioRxiv - Immunology 2021Quote: ... Concentrations of antigens were determined using standard curves constructed with purified recombinant proteins and calculated with Gen5 3.02.2 (BioTek).
-
bioRxiv - Cell Biology 2022Quote: ... 0.5 mM DTT) into the protein mix using the automated dispenser of a Synergy 4 microplate reader (BioTek) to a final volume of 200 μl and a final concentration of 2 μM actin in 1x KMEI with 0.05% antifoam ...
-
bioRxiv - Bioengineering 2020Quote: ... Reactions for the fluorescent proteins were carried out in a Synergy Neo2 HTS Multi-Mode Microplate Reader (BioTek) at 282 cpm.
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... Protein quantification of exosome and HCQ@ZnS@exo@iRGD were done by using Bradford assay (BioTek, Winooski, USA). The stability of the vesicles was studied by monitoring the change in size and PDI over a 7-day period.
-
bioRxiv - Immunology 2020Quote: ... unspecific protein binding sites were blocked by incubating sections with PBS containing 10% normal donkey serum (Pan-Biotek), 2% BSA and 0.2% Triton-X100 for 1 hour ...
-
bioRxiv - Biophysics 2022Quote: In vivo and in vitro spectra of all proteins were recorded using Synergy H4 Hybrid Microplate Reader (BioTek) with resolution of 9 nm ...
-
bioRxiv - Molecular Biology 2023Quote: ... and the total protein in each extract was estimated by reading the OD280nm using the spectrophotometer (Epoch, BioTek). For the Western blot ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... Total protein was quantified by absorbance at wavelength 280 nm using a Take3 microvolume plate (BioTek Synergy HT) and 1 μg was boiled for 10 min in 1x Laemmli Sample Buffer (BioRad ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... Total protein was quantified by absorbance at wavelength 280 nm using a Take3 microvolume plate (BioTek Synergy HT). In assay buffer ...
-
bioRxiv - Biochemistry 2020Quote: ... Low-affinity complexes and unbound proteins were removed in consecutive washing steps with PBS-T 0.05% (EL406 washer, BioTek). Beads were incubated for 10 minutes with 0.4% paraformaldehyde ...
-
bioRxiv - Plant Biology 2019Quote: ... and the fluorescence intensity of Venus-tagged protein was measured on a Synergy H4 Hybrid reader (Biotek, Winooskit, VT, USA) at excitation and emission wavelengths of 485 nm and 528 nm ...
-
bioRxiv - Cell Biology 2020Quote: ... Protein concentration was determined by taking OD at 280 nm in Nano drop (Synergy H1 Hybrid Multi-Mode Reader Biotek) and mass extinction co-efficient of the proteins.
-
bioRxiv - Neuroscience 2021Quote: ... The solubilized protein was quantified by BCA Assay Kit (Fisher) in triplicates at 590nm using a microplate reader (Synergy HT, BioTek). 15 to 20 µg of ß-mercaptoethanol denatured protein were loaded in Tris-glycine polyacrylamide gels (14% ...
-
bioRxiv - Microbiology 2020Quote: The TreS activity domain of TreS-Mak protein was monitored following the conversion of trehalose into maltose and glucose using Epoch spectrophotometer (Biotek). 15µL of reaction sample were incubated 30 min at 58°C with 30 µL of 100 mM sodium citrate pH 4.6 containing whether 0.4 U of amyloglucosidase from Aspergillus niger (Megazyme ...
-
bioRxiv - Neuroscience 2020Quote: ... All assays were allowed to incubate at 37°C for 30 min before protein concentration was read in at 562 nm (SynergyMix, Biotek).
-
bioRxiv - Cancer Biology 2022Quote: ... Protein quantification was performed using the Pierce BCA protein assay kit (ThermoScientific) and analyzed on a Cytation 3 plate reader (BioTek). Proteins were separated by NuPAGE 4-12% Bis-Tris Gel and transferred to a nitrocellulose membrane ...
-
bioRxiv - Cancer Biology 2022Quote: ... Cell and tissue debris was removed by centrifugation at 16,000x g for 5 minutes at room temperature and protein concentration was measured using A280 values determined by Cytation 5 (BioTek). Lysates were normalized in Buffer A with 1% SDS and prepared with 6X Laemmli sample buffer containing 100 mM 2-mercaptoethanol (Sigma M6250) ...
-
bioRxiv - Microbiology 2023Quote: ... Fluorescence of the Amcyan and Turborfp proteins produced by the spot colonies from each well was measured using the Synergy H1 plate reader (BioTek). We used the constitutive fluorescence of Amcyan to normalize TurboRFP production by reporter expression ...
-
bioRxiv - Microbiology 2023Quote: ... The green fluorescent protein (GFP)-positive cells were then counted using Cytation 5 with Gen5 v3.03 software (BioTek, Winooski, VT). Dose-response curves were plotted and EC50 values determined using Prism 9 (GraphPad ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cell debris was removed by centrifugation at 16,000x g for 5 minutes at room temperature and protein concentration was measured using A280 values determined by Cytation 5 (BioTek). Lysates were normalized in Buffer A with 1% SDS and prepared with 6X Laemmli sample buffer containing 100 mM 2-mercaptoethanol (Sigma M6250) ...
-
bioRxiv - Bioengineering 2019Quote: ... emission at 517 nm) of the membrane and cytoplasmic proteins was determined using fluorescence spectrophotometer (Synergy H4; BioTek, Winooski, VT, USA).
-
bioRxiv - Neuroscience 2021Quote: ... Plasma osmolality was analyzed by vapor pressure osmometry (VAPRO 5520) and volemia was indirectly inferred by the plasma protein concentration which was measured in an absorbance microplate reader (BioTek EL800), according to the protocol proposed by Lowry et al ...
-
bioRxiv - Synthetic Biology 2022Quote: The fluorescence intensity of the synthesized fluorescent proteins was measured by the multi-well plate fluorometer (Synergy HTX, BioTek, Winooski, VT). 5 µL of the cell-free synthesized fluorescent protein and 45 µL of Milli-Q water were mixed in a 96-well half-area black plate (Corning Incorporated ...
-
bioRxiv - Biophysics 2023Quote: Assessment of the extent of protein oligomerization was based on monitoring absorbance at 550 nm using BioTek Plate Reader (Agilent BioTek NEO2) to reflect turbidity changes induced by protein oligomer formation ...
-
bioRxiv - Bioengineering 2020Quote: ... Red fluorescent protein (RFP) readings (excitation 580 nm, emission 610 nm) were taken using a BioTek Synergy H1m plate reader (BioTek, Winooski, VT) after 18 hours of incubation at 37 °C with 200 rpm shaking.
-
bioRxiv - Immunology 2019Quote: ... The supernatant was subjected to a BCA protein assay (Pierce Biotechnology, Waltham, MA) and measured on a Synergy H1 Hybrid Multi-Mode Reader (BioTek, Winooski, VT). After normalizing the samples to the lowest sample concentration through the addition of PBS ...
-
bioRxiv - Microbiology 2022Quote: ... The absorbance of each well with reaction mixture and protein (but without formaldehyde) was measured at 340 nm with a BioTek Synergy H1 plate reader (BioTek, Winooski, VT) in a Corning CoStar 48 well plate (Corning ...
-
bioRxiv - Neuroscience 2022Quote: ... which used bovine serum albumin standards to create a best fit linear regression line based on protein concentrations using the 562nm absorbance mode of a microplate reader and its Gen5 Data Analysis program (Biotek, Winooski, VT). Equal amounts of protein were resolved in SDS-polyacrylamide gels and electrophoretically transferred to nitrocellulose membranes (Bio-Rad ...