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Citations for Biotek :
151 - 200 of 256 citations for RNA Amplification kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... The concentration of the isolated RNA was determined by measuring the absorbance at 260 nm wavelength with a spectrophotometer (Epoch, Biotek). The ratio of A260/A280 was between 1.9 and 2.0 ...
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bioRxiv - Cancer Biology 2022Quote: ... The concentration of the isolated RNA and the ratio of absorbance at 260 nm to 280 nm (A260/A280 ratio) were measured with spectrophotometer (Biotek). cDNA was generated using iScript Kit (Bio-Rad ...
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bioRxiv - Biochemistry 2019Quote: ... 1 μg of total RNA (purity/integrity assessed by agarose gel electrophoresis and concentration determined by A260nm using a BioTek Cytation3 microplate reader with a Take3 accessory ...
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bioRxiv - Cancer Biology 2019Quote: ... RNA and cDNA quality and quantity were checked using Synergy™ H1 Multimode Microplate Reader (BioTek Instruments, Inc., VT, USA). qPCR analysis was performed using iTaq Universal SYBR Green Supermix (BIO-RAD ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... RNA concentrations were determined at 260nm using a Take3 microvolume plate on a Synergy H1m microplate reader (Biotek, Winooski, Vermont). RNA samples (1 μg ...
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bioRxiv - Biochemistry 2023Quote: ... The quality and concentration of the input RNA were measured with a Synergy HT Take3 Microplate Reader (BioTek, Winooski, VT), and cDNA was prepared using SuperScript IV ...
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bioRxiv - Synthetic Biology 2021Quote: ... The amplicon was then purified using a Cycle Pure Kit (Omega Biotek). The vector backbone fragment was amplified from pET28a-M-CaM-cTdTS plasmid DNA constructed above using PfuUltra II Hotstart PCR Master Mix using DNA primers P29 – P36 listed in Table 1 ...
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bioRxiv - Cell Biology 2022Quote: ... and DNase-treated using commercially available kits (Omega Biotek and Ambion, respectively). Libraries were constructed and subsequently subjected to 151bp paired-end cycles on the NovaSeq-6000 platform (Illumina) ...
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bioRxiv - Synthetic Biology 2023Quote: ... PCR product was subsequently cleaned up using PCR cleanup kit (Omega Biotek) and stored in dH2O ...
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bioRxiv - Synthetic Biology 2023Quote: ... The product was subsequently cleaned up using PCR cleanup kit (Omega Biotek) and stored in dH2O ...
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bioRxiv - Microbiology 2021Quote: ... The quality (A260/A280) and quantity of extracted RNA was determined by nanodrop (Synergy™ HTX Multi-Mode Microplate Reader, Biotek). Next ...
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bioRxiv - Neuroscience 2021Quote: ... Purified total RNA was quantified on a Synergy 2 Multimode Microplate Reader using a Take3 micro-volume plate (BioTek, Winooski, VT). For cDNA synthesis ...
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bioRxiv - Cell Biology 2024Quote: ... Concentration and purity of the RNA was determined using a BioTek PowerWave XS microplate reader (BioTek Instruments Inc., Winooski, VT, USA). Library preparation of mRNA was done using Poly A enrichment ...
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bioRxiv - Immunology 2023Quote: ... The purified total RNA was eluted with 50 μL of elution buffer and quantified by absorbance using an Epoch spectrophotometer (BioTek, USA). Total RNA integrity was assessed by 1% agarose gel electrophoresis using 1× TAE buffer prepared with DEPC water.
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bioRxiv - Molecular Biology 2023Quote: ... The RNA concentration and purity were measured using HTX Synergy Multi-Mode Microplate Reader spectrophotometer (Agilent BioTek, BioTek Instruments Inc, USA). RNA integrity was checked by 1% agarose gel electrophoresis stained with ethidium bromide (see supplementary material for more detailed protocol) ...
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bioRxiv - Molecular Biology 2023Quote: ... The RNA concentration and purity were measured using HTX Synergy Multi-Mode Microplate Reader spectrophotometer (Agilent BioTek, BioTek Instruments Inc, USA). RNA integrity was checked by 1% agarose gel electrophoresis stained with ethidium bromide (see supplementary material for more detailed protocol) ...
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bioRxiv - Cell Biology 2020Quote: ... after genome extraction from larva or adults (E.Z.N.A insect DNA kit, Omega Biotek).
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bioRxiv - Synthetic Biology 2021Quote: ... The approximately 8100bp amplicon was purified using a gel extraction kit (Omega Biotek). DNA concentration of both linear fragments was measured using the Take3 plate coupled with a Biotek Cytation 5 plate reader ...
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bioRxiv - Systems Biology 2021Quote: ... coli DH10B cells and isolated with E.Z.N.A FastFilter Plasmid Maxiprep kit (Omega Biotek) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2020Quote: ... and PCR products were purified using the EZNA Gel Extraction kit (Omega BioTek).
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bioRxiv - Microbiology 2022Quote: ... gel extracted (E.Z.N.A.® Gel Extraction Kit, Omega Biotek, Norcross, GA, United States) and sent to an external lab for Sanger sequencing (Genewiz from Azenta Life Science ...
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bioRxiv - Bioengineering 2023Quote: ... and purified by DNA purification and gel extraction kits (Omega Biotek, GA, USA). For the ligation-dependent cloning ...
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bioRxiv - Molecular Biology 2023Quote: Genomic DNA was extracted using the Genomic DNA Isolation Kit (Norgen, Biotek Corp.) according to the manufacturer’s instructions and quantified using a spectrophotometer/fluorometer (DeNovix) ...
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bioRxiv - Genomics 2024Quote: ... DNA was extracted from muscle using the E.Z.N.A Tissue DNA kit (Omega Biotek) and eluting it in 100 µL ...
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bioRxiv - Neuroscience 2021Quote: ... Uptake of MEs labeled with FM 4-64 and RNA cargos labeled with ExoGlow-RNATM was quantified by using a microplate fluorescence reader (BioTek Instruments, Inc.) and confocal microscopy imaging respectively ...
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bioRxiv - Physiology 2019Quote: ... and an aliquot of the synthesized RNA probes were then visually assessed using standard agarose gel electrophoresis and quantified on a SYNERGY 2 Microplate reader (Biotek, Winooski, VT).
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bioRxiv - Physiology 2019Quote: ... The final purified PCR products for use as templates for RNA probe synthesis were quantified on a SYNERGY 2 Microplate reader (Biotek, Winooski, VT).
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bioRxiv - Immunology 2019Quote: ... the upper aqueous layer following phase separation was mixed with an equal volume of 75% ethanol and transferred to EZNA RNA isolation columns (Omega Biotek, Norcross, GA). iScript(tm ...
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bioRxiv - Microbiology 2020Quote: ... The RNA was quantified by spectrophotometry using a TAKE3 plate in a Cytation 5 multi-mode plate reader (Biotek, Winooski, VT, USA), and RNA quality was assessed by analysis of ribosomal RNA bands visualized following gel electrophoresis by SYBR-safe dye incorporation ...
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bioRxiv - Molecular Biology 2024Quote: ... The RNA concentration and purity were assessed using the HTX Synergy Multi-Mode Microplate Reader spectrophotometer (Agilent BioTek, BioTek Instruments Inc, USA). To verify RNA integrity ...
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bioRxiv - Molecular Biology 2024Quote: ... The RNA concentration and purity were assessed using the HTX Synergy Multi-Mode Microplate Reader spectrophotometer (Agilent BioTek, BioTek Instruments Inc, USA). To verify RNA integrity ...
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bioRxiv - Microbiology 2019Quote: ... and quantified by Quant-iT PicoGreen dsDNA Assay Kit on Microplate reader (BioTek, FLx800). Sequencing and data analysis were subsequently performed on an Illumina MiSeq platform by Personal Biotechnology Co. ...
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bioRxiv - Cancer Biology 2021Quote: ... The Assay and Universal primers supplied with Norgen’s HR kit (Norgen’s Biotek Corp., Canada) were used during both PCR and RT-PCR reaction set up ...
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bioRxiv - Microbiology 2021Quote: ... qPCR analysis was performed using a SYBR Premix Ex Taq kit (TaKaRa, Biotek, Dalian) and an ABI 7500 system under the following conditions ...
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bioRxiv - Synthetic Biology 2022Quote: ... and then purified by DNA purification and gel extraction kits (Omega BioTek, GA, USA). For the ligation-dependent cloning ...
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bioRxiv - Plant Biology 2023Quote: ... Fungal DNA was extracted using E.Z.N.A HP plant DNA Mini kit (Omega Biotek, USA). The DNA was sent for library construction with TruSeq DNA PCR-free library prep kit with 350-bp insert (Illumina ...
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bioRxiv - Immunology 2021Quote: ... Total RNA was quantified using the Take3 microvolume plate accessory on a Cytation 5 Cell Imaging Multi-Mode Reader (Biotek, Winooski, United States), RNA purity was determined by analyzing A260/280 and A260/230 ratios ...
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bioRxiv - Microbiology 2020Quote: ... RNA concentration and quality were measured using a biophotometer and on multimode spot reader using a Cytation™ 5 (BioTek Inc. Winooski, VT). Total RNA from different samples were used to synthesize cDNA using Superscript II reverse transcription kit (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... For each plate plasmids were extracted using the E-Z 96 FastFilter Kit (Omega BioTek) and eluted into a half-area UV transparent 96 well plate (Corning Costar) ...
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bioRxiv - Immunology 2020Quote: ... Plasmids were purified from positive DH5α colonies with ENZA plasmid DNA mini kit (Omega Biotek) and submitted to Genewiz for sequencing ...
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bioRxiv - Biochemistry 2022Quote: ... The product was purified on a spin column (EZNA Cycle Pure kit from Omega BioTek). A second PCR reaction was used to add the index (for pooled sequencing ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Omega Bioservices isolated genomic DNA using the E-Z 96 Tissue DNA Kit (Omega Biotek), made picogreen DNA sample quality checks ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We extracted genomic DNA from leaf tissue using the E.Z.N.A.® Plant DNA Kit (Omega Biotek), following manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2019Quote: ... Plasmids were isolated from subcultures using a DNA miniprep kit following the manufacturer’s instructions (Omega BioTek) and were Sanger sequenced to identify correctly assembled plasmids.
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bioRxiv - Molecular Biology 2019Quote: ... genomic DNA was also extracted from the pool of transfected cells using an E.Z.N.A Tissue DNA Kit (Omega Biotek). Translocation frequencies were estimated by serial dilution as previously described (Renouf et al. ...
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bioRxiv - Cancer Biology 2022Quote: ... and plasmid DNA was isolated using OMEGA’s EZNA plasmid DNA miniprep kit (Omega Biotek Inc Cat# D694202). Lentivirus containing supernatants were produced in packaging (293T ...
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bioRxiv - Genomics 2022Quote: Cell-free DNA was extracted from plasma using the Magbind ccfDNA extraction kit (Omega Biotek cat#M3298). Manufacturer recommendations for extraction were followed ...
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bioRxiv - Immunology 2020Quote: ... Samples were analyzed by agarose gel electrophoresis and purified PCR products (ENZA cycle pure kit; Omega Biotek) were cloned into the pCR2.1 vector using the Original TA cloning kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: Samples were prepared and DNA was extracted using the E.Z.N.A Soil DNA kit (Omega Biotek, Norcross, GA, USA). The tips of the swabs were placed in disruptor tubes by using a sterilized scissor ...
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bioRxiv - Genomics 2019Quote: ... Haploid megagametophyte tissue was used for genomic DNA material extraction by using E.Z.N.A.® SP Plant DNA kit (Omega Biotek). DNA was fragmented to an average length of 200 nucleotides with Bioruptor® ultrasonicator ...