Labshake search
Citations for Biotek :
51 - 100 of 123 citations for 4 1BBR TNFRSF9 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2020Quote: ... and fluorescence was measured 4 h-post addition using a Synergy HTX Multi-mode plate reader (BioTek). The metabolically active cells convert the blue redox reagent into its fluorescent product with the number of live cells directly proportional to the intensity of the fluorescent product ...
-
bioRxiv - Pathology 2021Quote: ... OD600 was measured every hour for 24 hours in a BioTek Synergy 4 microplate reader (BioTek-Agilent). In vitro growth graphs were generated using ggplot2 and ggprism ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... Culture OD600 was recorded every 4 minutes over 24 hours in an Epoch 2 plate reader (BioTek) set to 300 rpm and 30°C.
-
bioRxiv - Bioengineering 2022Quote: ... The absorbance (optical density) was read at 430 nm on the spectrophotometer (Biotek Synergy 4-plate reader). A standard curve of the absorbance of the serially diluted standards from 1.4 mg/ml to 50 ng/ml was used to calculate the sample concentrations ...
-
bioRxiv - Molecular Biology 2024Quote: Fluorescence polarization (FP) method was used to measure the binding affinity with Synergy 4 Microplate Reader (BioTek). Aliquots (5 nM ...
-
bioRxiv - Bioengineering 2024Quote: ... Approximately 20 ug of protein from each sample was resolved on a 4-20% gradient gel (Biotek), then visualized by SimplyBlue SafeStain.
-
bioRxiv - Immunology 2023Quote: ... Blank corrected samples and standard values were plotted using the 4-Parameter logistic model (Gen5 v3.09, BioTek).
-
bioRxiv - Cancer Biology 2020Quote: ... Magnetic cell sorting was performed to separate purified human versus mouse cell fractions using the Mouse Cell Depletion Kit (Miltenyi Biotek, 130-104-694). For the proteomic experiments ...
-
bioRxiv - Molecular Biology 2020Quote: Fluorescence polarization (FP) method was used to measure the binding affinity using a Synergy 4 Microplate Reader (BioTek). Aliquots (5 nM ...
-
bioRxiv - Cell Biology 2022Quote: ... 0.5 mM DTT) into the protein mix using the automated dispenser of a Synergy 4 microplate reader (BioTek) to a final volume of 200 μl and a final concentration of 2 μM actin in 1x KMEI with 0.05% antifoam ...
-
bioRxiv - Cell Biology 2019Quote: ... Plates were incubated for 18h before fixation (4% PFA) and staining using an automated plate washer (Biotek EL406). Cells were then incubated for 30min with Celltracker green and stained as follows ...
-
bioRxiv - Microbiology 2019Quote: ... and then optical density was monitored for 48 hours in an Biotek Synergy 4 plate reader (BioTek, USA) at 30 °C with fast continuous shaking ...
-
bioRxiv - Microbiology 2021Quote: ... Images were captured as a 5×4 matrix and GFP positive cells were enumerated using Gen5 software (Biotek).
-
bioRxiv - Genetics 2021Quote: ... The fluorescent intensity was then quantified (Ex/Em = 533/587 nm) using a microplate reader (BioTek Synergy 4). For measuring the ATP level ...
-
bioRxiv - Immunology 2022Quote: ... Fluorescence images of the entire well were acquired with a 4 X objective in a Cytation 5 (BioTek). The total number of cells indicated by the nuclei staining and the infected cells indicated by the NP staining were quantified with the cellular analysis module of the Gen5 software (BioTek) ...
-
bioRxiv - Biochemistry 2023Quote: ... Assay was incubated for 45 min at room temperature and plates were read using BioTek Synergy 4 (BioTek) plate reader ...
-
Lactylation-driven FTO-mediated m6A modification of CDK2 aggravates diabetic microvascular anomaliesbioRxiv - Cell Biology 2023Quote: ... The m6A level was then determined using a Synergy 4 automatic microplate reader (Agilent BioTek; Winooski, VT, USA) at 450 nm.
-
bioRxiv - Microbiology 2023Quote: ... The Formazan absorbance of each sample was measured at a 450 nm wavelength using a Synergy 4 (BioTek) microplate spectrophotometer ...
-
bioRxiv - Systems Biology 2024Quote: ... The absorbance at 450 nm was then measured every two minutes for 4 hours on Cytation 5 (Biotek) in 96-well plate ...
-
bioRxiv - Immunology 2021Quote: ... The results were measured on the SynergyTM 4 Multi- Mode Microplate Reader with software GEN5CH 2.0 (BioTek, Winooski, VT).
-
bioRxiv - Cell Biology 2019Quote: ... a Lionheart FX automated microscope in combination with sirDNA77 staining was used to generate growth curves with a time resolution of 4 hours for a total time span of 136 hours (microscope maintained at 37°C, 5% CO2 using a 4× lens and a Sony CCD, 1.25 megapixel camera with 2 times binning; BioTek). Quantification of cell number was performed by Gen5 software (BioTek).
-
bioRxiv - Microbiology 2020Quote: ... The 96-well plates were incubated at 37°C for 16 hours using the BioTek synergy 4 (BioTek, USA) plate reader ...
-
bioRxiv - Microbiology 2020Quote: ... Bacterial growth was monitored once per hour for 24 h after 30 sec linear shaking (Eon + Biostack 4, Biotek) under anaerobic conditions ...
-
bioRxiv - Microbiology 2019Quote: ... and optical density and fluorescence were monitored for 48 hours in a Biotek Synergy 4 plate reader (BioTek, USA) at 30 °C with fast continuous shaking ...
-
bioRxiv - Microbiology 2020Quote: ... the plate was further incubated at room temperature for 4 minutes and read by a spectrophotometer (BioTek, VT, USA) using a 540nm filter.
-
bioRxiv - Microbiology 2020Quote: ... was added to each well to stop the reaction and the optical density (OD) was measured at 492 nm on a Synergy 4 plate reader (BioTek). An average of OD values for blank wells plus three standard deviations was used to set a cutoff for plate blank outliers ...
-
bioRxiv - Microbiology 2019Quote: ... The resazurin was diluted at 0.004% in regular D10 medium and incubated with host cells at 37°C with 5% CO2 for 4 h before absorbance measurement at 570 and 600 nm by BioTek H1 Hybrid plate reader ...
-
bioRxiv - Microbiology 2019Quote: ... The plates were sealed with a semi-permeable film (Thermo Fischer) and incubated at 30°C in a Synergy 4 plate reader (BioTek) with shaking and absorbance readings (600 nm ...
-
bioRxiv - Biochemistry 2019Quote: ... Enzyme velocity was determined by measuring the formation of the 4-nitrophenolate product of hydrolysis (405 nm) for four minutes at room temperature using an Epoch microplate spectrophotometer (BioTek). Initial rates were corrected for non-enzymatic hydrolysis and the Michaelis constant was determined by fitting the initial rates to the Michaelis-Menten equation using GraphPad Prism (Supplementary Fig ...
-
bioRxiv - Cell Biology 2021Quote: ... Changes in intracellular Ca2+ concentrations were measured by recording the ratio of fluorescence intensities at 508/20 nm resulting from excitation of Fura-2 AM at 340/11 nm or 380/20 nm (center/bandpass) using a Synergy 4 multimode microplate reader (BioTek). Ratios were acquired every 0.7 seconds for 15 seconds before injection and 2 minutes after injection ...
-
bioRxiv - Microbiology 2020Quote: ... and then optical density and fluorescence were monitored for 48 hours in an Biotek Synergy 4 plate reader (BioTek, USA) at 30 °C with fast continuous shaking ...
-
bioRxiv - Cell Biology 2022Quote: ... Intracellular Fluo-4 AM was excited using automatic high-throughput living cell imaging and analysis system (BioTek, Lionheart FX, USA) at 494 nm ...
-
bioRxiv - Synthetic Biology 2019Quote: ... and then optical density and fluorescence were monitored for 48 hours in a Biotek Synergy 4 plate reader (BioTek, USA) at 30 °C with fast continuous shaking ...
-
bioRxiv - Synthetic Biology 2019Quote: ... and then optical density and fluorescence were monitored for 48□hours in an Biotek Synergy 4 plate reader (BioTek, USA) at 30□°C with fast continuous shaking ...
-
bioRxiv - Biochemistry 2019Quote: ... Decrease in Cy5 fluorescence was monitored by measuring fluorescence at 680 nm by excitation at 635nm on a Synergy 4 plate reader (BioTek).
-
bioRxiv - Biochemistry 2019Quote: Fluorescence anisotropy experiments were carried out at room temperature with 5 nM fluorescein (6FAM)-labelled DNA substrates (Table S2) using a Synergy 4 plate reader (BioTek). Titration with protein was performed by serially diluting protein in 40 μL of assay buffer (20 mM Tris Acetate pH 7.0 ...
-
bioRxiv - Microbiology 2020Quote: ... the reaction was stopped with 50 μls of 3M hydrochloric acid and absorbance was measured at 490 nm (OD490) using a Synergy 4 (BioTek) plate reader ...
-
bioRxiv - Microbiology 2022Quote: ... Fluorescence of released 4-methylumbelliferone (4MU) was measured (Excitation 360 nm, Emission 450 nm) in a plate reader (Synergy MX, Biotek). One unit of chitinase activity releases 1 μmol of 4MU from the appropriate substrate per minute ...
-
bioRxiv - Bioengineering 2022Quote: ... Pellet was then resuspended in 1 mL of M9 medium and flasks were inoculated to the starting OD600 of 0.1 and incubated at 30 °C with agitation (233 RPM for cultures with substrate concentration 4 g/L and 275 RPM for cultures with substrate concentration 8 g/L) using NB205 orbital incubator (N-Biotek). The ratio of medium to flask volume was 1:5 (20 mL:100 mL ...
-
bioRxiv - Microbiology 2022Quote: ... Plates were incubated at RT for 10 minutes in the dark before luminescence was measured using a Synergy 4 (BioTek) plate reader ...
-
bioRxiv - Neuroscience 2023Quote: ... Absorbance at 490 nm was measured 4 hours after the addition of the MTS dye using a SynergyNEO2 plate reader (BioTek).
-
bioRxiv - Physiology 2023Quote: ... The cells are first washed from their culture medium by 4 washes of KRB-BSA with the EL406 washer distributor liquid handling device (Biotek) suitable for 384-well plates ...
-
bioRxiv - Cancer Biology 2023Quote: ... incubated for 4 h and fluorescence was read at 550 nm/590 nm on a Cytation 3 Imaging Reader (BioTek).
-
bioRxiv - Microbiology 2023Quote: ... The reading process for multiple plates is automated using a BioStack™ 4 microplate stacker (BioTek Instruments, Inc., Winooski, VT), which cycles the experimental 96-well microplates and removes the lids at the specified interval as to not interfere with spectrophotometric values ...
-
bioRxiv - Microbiology 2023Quote: ... supernatant or size exclusion purified vesicles were extracted with butanol (1:2) overnight at 4°C and the butanol fraction transferred to microtiter plates for absorbance measurement (Synergy, Biotek).
-
bioRxiv - Microbiology 2024Quote: ... then samples were diluted (1:4) in PBS and quantified by measuring OD595 using a Synergy neo2 plate reader (BioTek).
-
bioRxiv - Immunology 2024Quote: ... Cytotoxicity were measured at 37°C and 5% CO2 every 10 min for 4 h using a Synergy HTX multi-mode plate reader (BioTek) at 485 nm excitation and 528 nm emission wavelengths in the bottom reader mode ...
-
bioRxiv - Microbiology 2019Quote: ... clear bottom 96-well plate and absorbance was measured at 425 nm in a Biotek Synergy 4 plate reader (BioTek, USA). A standard curve was made with bisdemethoxycurcumin standards dissolved in oleyl alcohol (Figure S3)
-
bioRxiv - Molecular Biology 2021Quote: ... The kinetics of substrate cleavage (increase in fluorescence of the liberated 7-amino-4-methylcoumarin, AMC) was measured using a fluorometer (BioTek, USA) set at 370 nm excitation and 460 nm emission at 37°C ...
-
bioRxiv - Immunology 2022Quote: ... Endpoint titers were determined by 4-parameter analysis using the Gen5™ 2.0 Data Analysis software (BioTek Instruments, Inc., Winooski, VT) and defined as the reciprocal of the serum dilution producing a signal identical to that of negative control samples at the same dilution plus three times the standard deviation.