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Citations for R&D Systems :
1 - 50 of 1868 citations for Siglec 15 Human HEK293 mFc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... HEK293-derived human Shh (R&D Systems, 8908-SH) served as a bioactivity control and to quantify Bosc23-expressed ...
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bioRxiv - Cancer Biology 2022Quote: ... live/dead stained and incubated with recombinant human Siglec-9 Fc chimera (#1139-SL-050) or recombinant mouse Siglec-E Fc chimera (#5806-SL-050) both from R&D systems, USA for 20 min on ice ...
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bioRxiv - Microbiology 2024Quote: ... neutrophils were incubated with anti-Siglec-9-APC (Clone 191240) and/or anti-Siglec-5/14-AF488 (Clone 194128) (R&D Systems) for 30 min ...
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bioRxiv - Immunology 2023Quote: ... Siglec-9 blocking antibody (Clone 191240, R&D Systems) was added at a final concentration of 10 µg/mL ...
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bioRxiv - Immunology 2023Quote: ... Siglec-9 blocking antibody (Clone 191240, R&D Systems) was added at a final concentration of 10 µg/mL ...
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bioRxiv - Immunology 2021Quote: ... 4 μg/mL of each Siglec-Fc (R&D Systems) was pre-complexed with either 8 μg/mL AffiniPure Donkey anti-human IgG Alexa Fluor 647 (Jackson ImmunoResearch ...
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bioRxiv - Immunology 2024Quote: ... human IL-15 (247-IL/CF; R&D Systems), human TGFβ1 (580706 ...
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bioRxiv - Systems Biology 2022Quote: ... human GDF-15 Quantikine ELISA Kit (DGD150; R&D Systems), human/mouse/rat Activin A Quantikine ELISA Kit (DAC00 ...
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bioRxiv - Bioengineering 2023Quote: ... 100 IU/ml recombinant human IL-15 (R&D Systems) and small molecule inhibitors 1 μM XL-413 and M-3814 (Selleck Chemicals) ...
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bioRxiv - Bioengineering 2023Quote: ... 100 IU/ml recombinant human IL-15 (R&D Systems). NFκB-reporter Jurkat cell line expressing cyan fluorescent protein (CFP ...
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bioRxiv - Bioengineering 2023Quote: ... 100 IU/ml recombinant human IL-15 (R&D Systems) and aCD3/aCD28 Expamers as previously described 27.
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bioRxiv - Cell Biology 2023Quote: ... human GDF-15 Duo Set (R&D Systems, cat.# DY957), human Serpin E1/PAI-1 Duo Set (R&D Systems ...
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bioRxiv - Biochemistry 2024Quote: ... 15 μg of human N1 antibody (AF5317: R&D Systems) was covalently coupled to 60 μl of Protein G-Dynabeads (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... the human GDF-15 ELISA Kit by R&D Systems was used ...
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bioRxiv - Immunology 2023Quote: ... Primary antibodies: goat anti-mouse Siglec-E (polyclonal IgG, R&D Systems) and mouse IgM anti-mouse Siglec-E (clone F-7 ...
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bioRxiv - Bioengineering 2021Quote: ... GDF-15 expression was analyzed using Human GDF-15 DuoSet® ELISA (DY957; R&D Systems™). For the activation of latent TGF-β1 ...
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bioRxiv - Immunology 2021Quote: ... and 2.5 ng/ml recombinant human IL-15 (R&D Systems). Mouse NK were isolated from splenocytes ...
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bioRxiv - Bioengineering 2024Quote: ... Human IL-15 Quantikine ELISA Kit (R&D Systems, MN, USA), 96-well ELISA plates (Corning ...
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bioRxiv - Immunology 2023Quote: ... Primary antibodies used were: goat anti-mouse Siglec-E (polyclonal IgG, R&D Systems) and mouse anti-cell membrane Hsp70 (clone 1H11 ...
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bioRxiv - Bioengineering 2020Quote: ... IL-15 superagonist was quantified using the human IL-15/IL-15R alpha complex DuoSet ELISA (R&D Systems DY6924). For BiTE experiments with primary human T cells ...
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bioRxiv - Bioengineering 2021Quote: ... IL-15 concentrations were determined by ELISA according to the manufacturer’s instructions (R&D Systems Human IL-15 Quantikine Assay). Absorbance was measured at 450 nm in a Synergy H1 Microplate Reader (BioTek) ...
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bioRxiv - Bioengineering 2021Quote: ... Serum IL-15 concentrations were determined by ELISA according to the manufacturer’s instructions (R&D Systems Human IL-15 Quantikine Assay). Absorbance was measured at 450 nm in a Synergy H1 Microplate Reader (BioTek) ...
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bioRxiv - Bioengineering 2021Quote: ... IL-15 concentrations were later determined by ELISA according to the manufacturer’s instructions (R&D Systems Human IL-15 Quantikine Assay). Absorbance was measured at 450 nm in a Synergy H1 Microplate Reader (BioTek) ...
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bioRxiv - Immunology 2023Quote: ... 5 µg/ml of recombinant mouse Siglec-E-Fc chimera (R&D Systems, Cat. 5806) in 100 μl of buffer (10mM Tris-HCl (pH7.4) ...
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bioRxiv - Cancer Biology 2020Quote: ... with C-terminal 6xHis tag produced in HEK293 cells and human recombinant PD-L1-Fc chimera protein produced in mouse NS0 cells were obtained from R&D Systems, Minneapolis ...
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bioRxiv - Cancer Biology 2022Quote: ... Anti– Siglec-9 antibody (5 μg mL-1; clone 191240, #MAB1139-500, R&D systems, USA) or human IgG isotype control (#31154 ...
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bioRxiv - Immunology 2020Quote: ... Recombinant human IL-15 and mouse IL-21 were from R&D Systems (Minneapolis, MN).
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bioRxiv - Bioengineering 2024Quote: The effective concentration of IL-15 in the samples was assessed using the Human IL-15 Quantikine ELISA Kit (R&D Systems, MN, USA). All subsequent steps after sample collections were carried out following the instructions provided by the kit ...
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bioRxiv - Physiology 2022Quote: GDF15 was measured in human plasma or serum using the Quantikine ELISA Human GDF-15 Immunoassay (ELISA, R&D systems, catalog no. DGD150). In mice ...
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bioRxiv - Physiology 2020Quote: GDF15 was measured in human plasma or serum using the Quantikine ELISA Human GDF-15 Immunoassay (ELISA, R&D systems, catalog no. DGD150). IL-10 was determined in single determinations using multi-spot immunoassay (V-PLEX ...
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bioRxiv - Immunology 2021Quote: ... recombinant mouse IL-15Rα-Fc and recombinant human IL-15 were purchased from R&D Systems. For each mouse ...
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bioRxiv - Physiology 2023Quote: ... and macaque serum using the Quantikine ELISA Human GDF-15 Immunoassay (ELISA, R&D systems, catalog no. DGD150). In mice and rats ...
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bioRxiv - Cancer Biology 2020Quote: ... The concentration of GDF15 was measured using an ELISA assay for Human GDF-15 DuoSet (R&D systems, # DY957) as per the manufacturer’s instructions on a Tecan INFINITE® 200 Pro spectrophotometer ...
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bioRxiv - Cancer Biology 2023Quote: ... for 10 minutes on ice and florescent antibody for 15 minutes on ice: PB2 PE (human, R&D Systems #FAB53291P), PB2 APC (human ...
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bioRxiv - Cancer Biology 2022Quote: ... bioreactor media were either left untreated or supplemented with anti– Siglec-9 antibody (5 μg mL-1; clone 191240, #MAB1139-500, R&D systems, USA). On day 5 ...
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bioRxiv - Immunology 2023Quote: ... Protein concentration was measured with BCA assay and 350 µg of lysates were incubated with 5 μg of anti-Siglec-E antibody (polyclonal goat IgG - R&D Systems – cat. AF5806) or goat IgG for 2h at 4°C ...
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bioRxiv - Microbiology 2021Quote: Monocyte-derived macrophages (MDMs) were obtained by stimulation of PBMC cultures with 15 ng/mL recombinant human M-CSF (R&D systems) and 10% human AB serum (Sigma Aldrich ...
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bioRxiv - Cell Biology 2021Quote: Isolated human platelets were incubated at room temperature for 15-20 minutes with either recombinant HMGB1 (0-40 μg/ml; R&D Systems), ADP (0-5 μM ...
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bioRxiv - Immunology 2020Quote: ... 5×106 PBMCs were combined with 5×106 irradiated BLCLs from MHC-identical donor MCMs in 5 ml of RPMI-1640 containing 15% FCS (R15) and 10 ng/ml recombinant human IL-7 (R&D Systems). Cultures were supplemented 2 days later with 1.25 ml of R15 containing 100 U/ml recombinant human IL-2 (R&D systems ...
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bioRxiv - Genetics 2023Quote: ... The calibrator supplied with the kit by Ansh Labs is traceable to recombinant human GDF-15 from R&D Systems (Biotechne, USA). Ansh Lab ELISA Total GDF15 between batch imprecision kit controls 7.7% at 173.2 pg/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... for 10 minutes on ice and incubated with fluorescence-conjugated antibodies for 15 minutes on ice: PB2 PE (phycoerythrin) (human, R&D Systems #FAB53291P), PB2 APC (human ...
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bioRxiv - Physiology 2021Quote: ... HOMA-IR was calculated as (fasting glucose × fasting insulin)/22.5 and HOMA-B as (20 × fasting insulin)/(fasting glucose – 3.5).(31) GDF15 concentration was measured with Human GDF-15 Quantikine Elisa Kit (R&D Systems, Minneapolis, MN, USA). Serum GDF15 samples during and after pregnancy were diluted 1:64 and 1:4 ...
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bioRxiv - Bioengineering 2021Quote: ... IL-15 (R&D Systems) was incorporated with the nanoparticles during hydrogel formulation.
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bioRxiv - Neuroscience 2021Quote: ... Media was then exchanged to DMEM containing 15 ng/mL recombinant human granulocyte-macrophage colony stimulating factor (rhGM-CSF; R&D Systems # 215-GM-010/CF). PBMC Mϕ were allowed to differentiate for an additional 5 days and were returned to DMEM 10% FBS until stimulation.
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bioRxiv - Immunology 2020Quote: ... and IL-15 (R&D Systems) for 48-56 hours ...
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bioRxiv - Cancer Biology 2022Quote: ... and IL-15 (R&D systems), and cultured at 37 °C with 5% CO2 for 24 hours ...
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bioRxiv - Cancer Biology 2022Quote: ... 20ng/μL IL-15 (R&D Systems), 20ng/μL IL-18 (R&D Systems) ...
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bioRxiv - Immunology 2024Quote: ... IL-15 (R&D Systems, 10ng/ml), IL-18 (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... human fibronectin and human recombinant BMP-2 (R&D systems, Biotechne, UK); recombinant human BMP-2 (Infuse/InductOS® Bone graft kit ...
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bioRxiv - Immunology 2023Quote: ... recombinant human PD-L1 human IgG1 Fc chimera protein (R&D Systems), or mouse IgG1 isotype antibodies (R&D Systems ...