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Citations for R&D Systems :
1 - 47 of 47 citations for SDS PAGE Single gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The Hoc proteins each eluted as a single monomeric peak and were run on reducing SDS-PAGE and verified by anti-His western (R&D Systems) (Supplementary Fig.3) ...
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bioRxiv - Cell Biology 2020Quote: ... and analyzed by SDS-PAGE and immunoblotting by using anti-Shh antibodies (R&D Systems, AF464). Signals were quantified with ImageJ and normalized to the highest protein amount detected in each run.
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bioRxiv - Physiology 2020Quote: Plamatic IgE level was evaluated by SDS PAGE using rat anti-mouse IgE antibodies (R&D systems Cat# MAB9935).
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bioRxiv - Physiology 2020Quote: ... Ocln and Cldn5 was evaluated by SDS PAGE using goat anti-mouse Cdh5 antibodies (R&D systems, cat#AF1002), mouse anti-Ocln (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... Ptch1 and Smo were evaluated by SDS PAGE using goat anti-mouse Cdon antibodies (R&D systems, Cat#AF2429), goat anti-human Gas1 antibodies (R&D systems ...
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bioRxiv - Cell Biology 2023Quote: Alkaline comet assays were performed using the CometAssay single cell gel electrophoresis assay kit (R&D systems) according to the manufacturers’ instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... eluted products and 10% of the input were separated by SDS-PAGE and transferred to a PVDF membrane for blotting at 4C with Gli3 (polyclonal goat IgG 1:1000, R&D Systems) and Hand2 (polyclonal goat IgG or mouse monoclonal IgG1 1:1000 ...
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bioRxiv - Cell Biology 2022Quote: ... followed by SDS-PAGE and Western blot analysis with a rabbit anti-GABAA receptor α1 subunit antibody (R&D Systems, catalog #: PS022), a mouse anti-GABAA receptor β2/β3 subunit antibody (Millipore ...
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bioRxiv - Developmental Biology 2024Quote: ... of medium samples were analysed by SDS-PAGE under reducing conditions and underwent Western blotting using anti-Wnt5a antibody (MAB6452, R&D systems). Immune signals for exogenously added Wnt5a in the medium and cell lysate were quantified using ImageJ ...
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Cardiac pericytes are necessary for coronary vasculature integrity and cardiomyocyte differentiationbioRxiv - Physiology 2022Quote: ICAM-1 and VCAM-1 protein levels were evaluated by SDS PAGE using goat anti-Icam-1 (R&D systems Cat# AF-796) and rabbit anti-Vcam-1 (abcam ...
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bioRxiv - Biochemistry 2024Quote: ... Eluate fractions were analyzed by SDS-PAGE and immunoblot using an anti-histidine tag antibody (Monoclonal Mouse IgG1 histidine tag antibody; R&D Systems, cat. no. MAB050). Fractions containing TDO2 were combined and dialyzed (molecular weight cutoff 3.5 kDa ...
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bioRxiv - Cancer Biology 2023Quote: ... We used a Comet Assay Single Cell Gel Electrophoresis Assay kit (cat. no. 4250-050-K; R&D Systems, Bio-Techne) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Cell lysates and corresponding supernatants were analyzed on the same reducing SDS polyacrylamide gel and detected by Western blot analysis by using goat-α-Shh antibodies (R&D Systems, AF464), rabbit-α-GAPDH antibodies (Cell Signaling ...
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bioRxiv - Neuroscience 2023Quote: ... The cell lysates (20 μg) were electrophoresis using SDS gels and transferred to nitrocellulose membranes and the incubated with TrkB antibody (1:1000, R&D systems, goat, AF1494), CRHR1 antibody (1:1000 ...
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bioRxiv - Immunology 2023Quote: ... and commercially available single cytokine ELISA kits (eBioscience, R&D Systems).
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bioRxiv - Cell Biology 2019Quote: ... recombinant syndecan-4 (R&D Systems Cat# 6267-SD-050) and recombinant syndecan-2 (R&D Systems Cat#6585-SD-050) ...
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bioRxiv - Cell Biology 2019Quote: ... and recombinant syndecan-2 (R&D Systems Cat#6585-SD-050), proteome profiler human phospho-kinase array kit (R&D Systems Cat#ARY003B) ...
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bioRxiv - Immunology 2020Quote: ... as described in detail ((16) or single-plex ELISA (R&D Systems, Minneapolis, MN). Analytes quantified using the Luminex® multiplex platform were read on the MAGPIX® instrument and raw data were analyzed using the xPONENT® software ...
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bioRxiv - Immunology 2023Quote: ... except using 250 ng/mL CXCL12 (R&D Systems, 460-SD-01/CF) instead of CCL21.
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bioRxiv - Cell Biology 2023Quote: ... single cell suspensions were stained with a goat anti-galectin-9 antibody (AF2045, R&D systems) at 40 μg/ml or isotype control as negative control for 30 min at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... single cell suspensions were stained with a goat anti-galectin-9 antibody (AF2045, R&D systems) at 8 μg/ml or isotype control as negative control for 30 min at 4 °C ...
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bioRxiv - Microbiology 2021Quote: Protein expression was analyzed by polyacrylamide gel electrophoresis on 8%-16% precast gradient gels (Thermo) and Western blotting using antibodies to ACE2 (AF933, R&D Systems), c-Myc-epitope (clone 9E10 ...
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bioRxiv - Cell Biology 2019Quote: ... gels including CXCL12 (R&D Systems, #350-NS/CF) or PDGFB (R&D Systems ...
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bioRxiv - Immunology 2019Quote: ... Single cell suspensions from intestinal lamina propria were stimulated with 40 ng/ml of IL-23 (R&D Systems) for 3 hours followed by 50ng/ml PMA ...
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bioRxiv - Immunology 2022Quote: ... single allele APCs were resuspended in CRPMI (w/o phenol red) +10% fetal bovine serum (FBS, R&D Systems) containing 1 µg/mL of each peptide and incubated overnight ...
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bioRxiv - Neuroscience 2023Quote: ... animals in the PE+PSB0739 group were subjected to intracerebroventricular injection (i.c.v) with a single dose of PSB0739 ((Tocris, R&D Systems, Minneapolis ...
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bioRxiv - Neuroscience 2021Quote: Single-cell RNA-seq analysis of cultured NSCs isolated from the cortex of E14.5 Sprague-Dawley rats (NSC001, R&D systems; cultured according to the manufactures’ protocol ...
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bioRxiv - Biochemistry 2023Quote: ... Single human recombinant DUBs (800 nM) were added together with 50 μM human recombinant HA-tagged ubiquitin (R&D SYSTEMS, U110) to each reaction ...
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bioRxiv - Bioengineering 2020Quote: ... Collagen gel loaded with BMP-2 (75 μg/mL, R&D Systems) was used as a positive control ...
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bioRxiv - Molecular Biology 2024Quote: ... Gels were probed with antibodies against hARSA (R&D systems, Minneapolis, MN), CD63 (Abcam ...
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bioRxiv - Molecular Biology 2021Quote: ... Single cell suspensions of wild type and Pds5a KO mESCs were permeabilized and fixed using the fixation/permabilization buffer (R&D systems), washed twice with 1x PBS and stained using the H/MM pluripotent Stem Cell Multi-Color Flow Cytometry kit (R&D Systems ...
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bioRxiv - Microbiology 2022Quote: ... Tissues were minced and digested with collagenase to single cells before embedded in reduced growth factor extracellular matrix (Cultrex BME2, R&D Systems). Selective isolation of ductal stem cells was achieved using Isolation Medium (IM ...
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bioRxiv - Immunology 2022Quote: ... The single memory B cells were cultured in complete RPMI 1640 medium supplemented with IL-2 (10 U/ml, R&D Systems), IL-21 (10 U/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... with good affinity and specificity were screened and selected from the homemade human single-chain antibody library through commercial VEGF and EGFR proteins (R&D Systems, Inc.). Then ...
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bioRxiv - Neuroscience 2019Quote: ... surgeries a single dose (0,6 mg/kg dissolved in phosphate buffer or saline) of P2Y12 receptor (P2Y12R) antagonist (PSB0739 Tocris, R&D Systems, Minneapolis, USA) was administered to the cisterna magna in 5 µl final volume using a glass capillary ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... resuspended in SDS loading buffer and subjected to Western Blot against phosphorylated Tyrosine (1:1000, BAM1676, R&D systems, Mineapolis, USA) or Traf6 antibody (1:2000 ...
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bioRxiv - Bioengineering 2022Quote: Transduced/Transfected and sorted hGICs harbouring either Lentivirus-MGT#1 or PiggyBac-MGT#1/4 were seeded as single cells into 6-well plates in RHB-A medium supplemented with 10ng/ml TNFa (R&D Systems, 210-TA-020) or without TNFa as control ...
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bioRxiv - Microbiology 2023Quote: ... organoids were mechanically disrupted into single cells and plated in droplets of Cultrex growth factor reduced BME type 2 (Biotechne | R&D systems 3533-010-02). For the 2D culture of airway organoids ...
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bioRxiv - Cell Biology 2022Quote: ... primary BMSCs were plated in 6-well dishes until 80% confluent and serum starved (0.5% FBS) for 72 hours prior to treatment with one or a combination of CXCL12 (200 ng/ml, R&D Systems, 460-SD) and Wnt3a (100 ng/ml ...
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bioRxiv - Cell Biology 2022Quote: ... primary BMSCs isolated from C57BL/6 mice were plated in 6-well dishes until 80% confluent and serum starved (0.5% FBS) for 48 hours prior to treatment with one or a combination of CXCL12 (200 ng/ml, R&D Systems, 460-SD), Wnt3a (100 ng/ml ...
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bioRxiv - Cell Biology 2022Quote: ... MC3T3-E1 Subclone 4 preosteoblasts (ATCC CRL-2593) grown in monolayer were treated with vehicle or CXCL12 (20 μg/ml, R&D Systems, 460-SD) for 4 hours ...
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bioRxiv - Biochemistry 2023Quote: ... the gel was incubated in 20 ml Buffer A supplemented with 20 μM Suc-LLVY-AMC (R&D Systems) at 30°C for 10 min and imaged under UV light ...
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bioRxiv - Cell Biology 2019Quote: ... Directional cell migration was induced by overlaying the polymerized gels with 0.63μg/ml CCL19 diluted in complete media (R&D Systems). To prevent drying-out of the gels ...
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bioRxiv - Cell Biology 2022Quote: ... The collagen-cell mixture was then casted in custom-made chamber and collagen was allowed to polymerize for 45min at 37°C before overlaying the gel with either 0.6ug/ml of recombinant mouse CCL19 (for DCs, 440-M3-025, R&D Systems), 25nM fMLP (for neutrophil-like cells ...
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bioRxiv - Microbiology 2022Quote: ... Gels were then subjected to immunoblot analysis for CHIKV E using an anti-E antibody (1:1000, R&D Systems, MAB97792SP).
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bioRxiv - Cell Biology 2020Quote: ... were covered with HUVECs and embedded 24 hours later in a 2,5 mg/mL fibrin gel in the presence or absence of VEGFA-165 at 5 ng/mL (R&D systems, Minneapolis MN, USA). Conditioned media from papillary or reticular fibroblasts were added on top of the gel and renewed every day for four days ...
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bioRxiv - Bioengineering 2020Quote: ... Microvascular fragment-containing gels were cultured in serum-free media supplemented with 10 ng/mL recombinant human vascular endothelial growth factor (rhVEGF; R&D Systems, Minneapolis, MN) (55) ...