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Citations for R&D Systems :
1 - 50 of 1910 citations for E3 Ubiquitin Protein Ligase DTX1 DTX1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... and 10x E3 Ligase Reaction Buffer (R&D systems B-71). Addition of Rik1-TAP complex purified from S ...
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bioRxiv - Molecular Biology 2022Quote: ... and buffered with 1X E3 Ligase Reaction Buffer (R&D systems, B-71). Substrates and Z-VAD-FMK (UBPBio ...
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bioRxiv - Molecular Biology 2023Quote: ... Cullin E3 ligase activity was blocked by supplementing egg extracts with 100 µM neddylation E1 enzyme inhibitor (MLN4924, R&D systems). Proteasome activity was inhibited via addition of 200 μM MG262 (Boston Biochem) ...
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bioRxiv - Biochemistry 2021Quote: ... Ubiquitin (R&D Systems) (1 mM Ub) ...
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bioRxiv - Molecular Biology 2020Quote: ... and BARD1 proteins was conducted using recombinant human ubiquitin (U-100H, R&D system, USA) and HeLa NE as a source of E1 and E2 ligases in 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Immunology 2023Quote: ITCH ubiquitination reactions were carried out using the manufacturer’s protocol for the Human ITCH/AIP4 Ubiquitin Ligase Kit (R&D Systems, K-270). 10X reaction buffer ...
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bioRxiv - Biochemistry 2023Quote: ... The SKP1 containing SCF complex was reconstituted by utilizing stoichiometric amount of the following recombinant proteins: Recombinant Human CUL1/RBX1 Neddylated Complex Protein (R&D systems, E3-411-025), Recombinant Human FBXO7 GST (N-Term ...
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bioRxiv - Biochemistry 2022Quote: ... 50 μM ubiquitin (R&D Systems), either 0.5 μM wildtype UBR5 or catalytically dead mutant UBR5(C2768S) ...
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bioRxiv - Molecular Biology 2022Quote: ... ubiquitin (R&D systems, U-100H) at 50 µM ...
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bioRxiv - Molecular Biology 2021Quote: ... purified 6xHis-Clr4 protein was mixed with human Ubiquitin-activating Enzyme (UBE1, R&D systems E-305), human UbcH5c/UBE2D3 (R&D systems E2-627) ...
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bioRxiv - Biophysics 2023Quote: ... Tagless VCB complex was purchased from R&D Systems (E3-600) excluding DTT in the supplied buffer to ensure compatibility with the His-Tag Dynabeads ...
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bioRxiv - Molecular Biology 2021Quote: ... human Ubiquitin (R&D systems U-100H) or human HA-Ubiquitin (R&D systems U-110) ...
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bioRxiv - Biochemistry 2020Quote: ... ubiquitin (R&D Systems; Cat. # U-100H), His-ubiquitin (R&D Systems ...
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bioRxiv - Biochemistry 2020Quote: ... ubiquitin (R&D Systems; Cat. # U-100H), His-ubiquitin (R&D Systems ...
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bioRxiv - Neuroscience 2024Quote: ... Ubiquitin-Rh110 (R&D Systems, #U-555) at 670 nM in phenol-free RPMI ...
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bioRxiv - Cell Biology 2020Quote: ... using recombinant carrier free ACE2 (# 933-ZN-010) and Skp1/Skp2 (#E3-521-025) proteins bought from R&D Systems (Minneapolis, MN, USA) or Flag-ACE2 and Flag-Skp1/Skp2 purified from 293T cells ...
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bioRxiv - Biochemistry 2020Quote: ... HA-ubiquitin (R&D Systems; Cat. # U-110) or GST-ubiquitin (R&D Systems ...
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bioRxiv - Biochemistry 2020Quote: ... His-ubiquitin (R&D Systems; Cat. # U-530), HA-ubiquitin (R&D Systems ...
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bioRxiv - Biochemistry 2020Quote: ... HA-ubiquitin (R&D Systems; Cat. # U-110) or GST-ubiquitin (R&D Systems ...
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bioRxiv - Biochemistry 2020Quote: ... His-ubiquitin (R&D Systems; Cat. # U-530), HA-ubiquitin (R&D Systems ...
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bioRxiv - Molecular Biology 2020Quote: ... and ubiquitin (U-100H, R&D system, USA) were added to 100 nM and 2 μM ...
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bioRxiv - Cancer Biology 2021Quote: ... 75 mM ubiquitin (#U-100H R&D systems) 1.6 mM FLAG-PCNA substrate (expressed and purified in bacteria) ...
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bioRxiv - Molecular Biology 2023Quote: ... 50 μM recombinant yeast ubiquitin (R&D Systems), 5 μM Rad6 or Rad6-150 ...
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bioRxiv - Molecular Biology 2019Quote: ... Recombinant human RNF4 was purchased from R&D Systems (Cat. # E3-210-050). Recombinant human ubiquitin was purchased from R&D Systems (Cat ...
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bioRxiv - Molecular Biology 2023Quote: ... Recombinant Biotin-tagged K48-linked poly-ubiquitin chains (2-7) and K48-linked poly-ubiquitin chains (2-16) were purchased from R&D Systems and Enzo Life Science ...
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bioRxiv - Molecular Biology 2021Quote: ... or human HA-Ubiquitin (R&D systems U-110), Mg-ATP (R&D systems B-20 ...
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bioRxiv - Biochemistry 2020Quote: ... or GST-ubiquitin (R&D Systems; Cat. # U-540) were solubilized in 50 mM HEPES (pH= 7.4) ...
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bioRxiv - Biochemistry 2020Quote: ... or GST-ubiquitin (R&D Systems; Cat. # U-540) were incubated in 20 µl standard kinase reaction buffer (Cell Signaling Technology ...
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bioRxiv - Biophysics 2023Quote: ... and either 100 μM yeast ubiquitin (R&D Systems) or 100 μM ubiquitinCy3 ...
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bioRxiv - Biochemistry 2020Quote: All proteins described herein for in vitro analyses were expressed and purified as recombinant proteins with the exception of E1 activating enzyme UBE1 and heavy isotope labeled ubiquitin (both purchased from R&D Systems). Recombinant ubiquitin ...
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bioRxiv - Molecular Biology 2021Quote: ... 2.5 µM recombinant yeast or human ubiquitin (R&D Systems), 0.1 µM wild-type or mutant Rad6 or UBE2B (E2) ...
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bioRxiv - Cell Biology 2021Quote: ... or GST-tagged-Ubiquitin (R&D systems, U-540-01M). Incubation was performed overnight at 4°C in 500 µL 50 mM Tris.HCl ...
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bioRxiv - Cell Biology 2022Quote: ... and 58 μM ubiquitin (R&D Systems, U-100H-10M) in 50 μl reaction buffer (20 mM Tris-Cl pH 7.6 ...
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bioRxiv - Cell Biology 2022Quote: ... 750 nM ubiquitin conjugating enzyme UBCH5B (R&D Systems, E2622100), 2 mM ATP ...
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bioRxiv - Molecular Biology 2022Quote: ... and ubiquitin (U-100H) were purchased from R&D systems. Components were mixed to final concentrations of 1 µM Ube1 ...
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bioRxiv - Microbiology 2023Quote: ... 1 μM ubiquitin-propargylamide (R&D Systems, Cat# U-214) was incubated with 100 nM SdeC variants at room temperature for 30 min before adding to Ub4 (final concentration ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.9 μg/μl of human ubiquitin (R&D systems U-100H) or buffer only (10 mM Hepes ...
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bioRxiv - Biochemistry 2020Quote: ... AQUA pure tetra ubiquitin chains were purchased from R&D Systems (Cat ...
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bioRxiv - Cancer Biology 2021Quote: ... 0.1 mM E1 Ubiquitin Activating Enzyme (#E-304 R&D systems), 0.2 mM E2 conjugase (UbcH5c ...
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bioRxiv - Biochemistry 2023Quote: ... 125 μM ubiquitin (yeast recombinant from bio-techne (R&D Systems)) ...
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bioRxiv - Biochemistry 2023Quote: ... FLAG-ubiquitin (0.5 μL, 20 mg/mL, R&D Systems, U12001M), MgCl2 (1.25 μL ...
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bioRxiv - Molecular Biology 2019Quote: ... Recombinant human ubiquitin was purchased from R&D Systems (Cat. # U-100H). Recombinant human ubiquitin-activating enzyme E1 (UBE1 ...
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bioRxiv - Biochemistry 2023Quote: ... 50 μM of human recombinant untagged ubiquitin (R&D SYSTEMS, U-100H) and specific human recombinant DUBs were added at the same concentration as for the proteomic experiments (800 nM) ...
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bioRxiv - Molecular Biology 2023Quote: ... HA-SUMO1 Vinyl Sulfone (VS) and HA-Ubiquitin VS (R&D Systems) were added to all reactions containing purified Strep-HA-TOPORS proteins in order to inhibit deubiquitinase and SUMO protease activities co-purifying with Strep-HA-TOPORS.
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bioRxiv - Biochemistry 2020Quote: AQUA mass spectrometry data for tetra ubiquitin was provided by R&D Systems. Lot # specific data can be obtained for K6 (Lot # 24369018A ...
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bioRxiv - Biochemistry 2023Quote: ... 23 µM methylated ubiquitin (U-501-01M; R&D System, Minneapolis, MN, USA) was pre-heated at 37°C for 20 min ...
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bioRxiv - Molecular Biology 2023Quote: ... the following final concentrations were used: FLAG Ubiquitin (R&D Systems, 20 μM), ATP (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2019Quote: ... Recombinant human ubiquitin-activating enzyme E1 (UBE1) was purchased from R&D systems (Cat. # E-305). Recombinant human UbcH5a was purchased from R&D systems (Cat ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by addition of 5 µl/0.5 µM Ubiquitin-AMC (R&D Systems #U-550-050) substrate ...
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bioRxiv - Molecular Biology 2019Quote: 10 μl in vitro ubiquitylation assay reactions in 1 x ubiquitin conjugation reaction buffer (R&D systems Cat. # B-70) contains 10 mM Mg2+-ATP solution pH 7.0 (R&D systems Cat ...