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Citations for R&D Systems :
1 - 50 of 1465 citations for 6 BROMO 3 4 DIHYDROQUINOLINE 2 1H THIONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Low-dose cytokine immunotherapy of solid cancers enabled by phagocytic-competent protein co-crystalsbioRxiv - Cancer Biology 2023Quote: ... 3 and 6 days and subsequently tested by IL-2 ELISA (R&D Systems) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2021Quote: ... After washing wells were incubated with a 5-bromo-4-chloro-3⍰-indolyl phosphate (BCIP)/nitro blue tetrazolium (NBT) substrate (R&D systems, mouse IFNγ Kit Cat # EL485). Plates were scanned and analyzed using ImmunoSpot Microanalyzer.
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bioRxiv - Cancer Biology 2021Quote: Cells were grown to 70% confluent in 24-well plates prior to treatment with N-[2-[[3-(4-Bromophenyl)-2-propenyl]amino]ethyl]-5-isoquinolinesulfonamide dihydrochloride (H89; R&D Systems) or 3-(1,3-Benzodioxol-5-ylmethylene)-2-oxo-1-pyrrolidinecarboxaldehyde (KNK437 ...
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bioRxiv - Microbiology 2021Quote: ... ULBP-2/5/6 (65903, R&D systems), Plexin-B1 (rea728 ...
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Direct intracellular visualization of Ebola virus-receptor interaction by in situ proximity ligationbioRxiv - Microbiology 2020Quote: ... or recombinant human cathepsin L (CatL, 2 ng/ul, pH 5.5, 37°C for 1h; R&D Systems) as described previously (5) ...
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bioRxiv - Physiology 2020Quote: ... with 2 µg of recombinant human TSG-6 (rTSG-6, R&D Systems, Minneapolis, MN) in 100 µl PBS (or with PBS alone ...
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bioRxiv - Cancer Biology 2021Quote: ... 3 µg/mL anti-ULBP2/5/6 (clone 165903, R&D Systems), and 6 µg/mL anti-ULBP3 (clone 166510 ...
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bioRxiv - Bioengineering 2022Quote: ... Supernatant was collected and cytokines were quantified using mouse cytokine & chemokine convenience 6-Plex kit (IL-2, IL-4, IL-5, IL-10, IFN-γ and TNF-α; # LXSAMSM-06, R&D systems) using Luminex xMAP according to manufacturers protocols.
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bioRxiv - Immunology 2020Quote: ... 3 and 4 were measured using Luminex technology (R&D Systems), according to the manufacturer’s protocols ...
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bioRxiv - Cell Biology 2020Quote: ... PE anti-human ULBP-2/5/6 (Clone 165903, R&D Systems), FITC Mouse IgG1 κ Isotype Control (Clone MOPC-21 ...
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bioRxiv - Immunology 2022Quote: IL-4 complexes were generated by incubating 2 μg mouse rIL-4 (R&D Systems) with 10 μg LEAF purified anti-mouse IL4 antibody (clone 11B11 ...
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bioRxiv - Cancer Biology 2023Quote: The blots were probed overnight at 4°C with monoclonal mouse anti-β-Catenin (2 µg/mL in Bovine Serum Albumin 3%, R&D Systems, Minneapolis, USA, #MAB1329), monoclonal rabbit anti-NDRG4 (1:1000 ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were cultured for 4 days with 3 μM CHIR (R&D Systems); 3 days with 200 ng/mL FGF9 (R&D Systems) ...
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bioRxiv - Immunology 2023Quote: ... 2 ng/mL of IL-2 and 3 ng/mL of TGFβ (R&D systems, 7666-MB-055)) conditions ...
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bioRxiv - Genomics 2021Quote: ... Polyclonal Goat Anti- Human/Mouse Oct-3/4 Antibody (R&D System, AF1759,1:20).
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bioRxiv - Cancer Biology 2024Quote: ... Secondary antibody was added for ULBP 1 and 2/5/6 only (R&D Systems). Cells were then washed and fixed in 4% paraformaldehyde ...
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bioRxiv - Cancer Biology 2022Quote: ... Digested cells were washed three times with PBS and resuspended in 2/3 Cultrex Type 2 (R&D Systems) and 1/3 Full Ad-DF+++ medium and plated in drops which were allowed to solidify for 30 minutes at 37°C after which they were overlayed with Full Ad-DF+++ medium ...
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bioRxiv - Neuroscience 2019Quote: ... and passaged every 4-6 day in the presence of 5 μM ROCK inhibitor (R&D Systems) until the 4th passage (P4) ...
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bioRxiv - Immunology 2021Quote: ... mice were injected subcutaneously with IL-4 complex (IL-4c) comprised of rmIL-4 (2 μg, R&D systems) combined with anti-IL-4 antibody 10 μg ...
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bioRxiv - Neuroscience 2021Quote: ... PlGF-2 (3 – 30 ng; 5 µl; cat.465-PL/CF, R&D System, USA), VEGF-E (3 – 30 ng ...
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bioRxiv - Cancer Biology 2022Quote: ... or the combination of both drugs in methylcellulose media enriched with human cytokines (recombinant human SCF, GM-CSF, G-CSF, IL-3, IL-6, Epo, #HSC005, R&D Systems). The methylcellulose mixture was plated in three replicates to 35 mm culture dishes using regular syringes with 16 gauge non-stick needles and incubated at 37°C ...
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bioRxiv - Bioengineering 2020Quote: ... Mouse IL-28 A/B (IFN-lambda 2/3) Du-oSet ELISA (DY1789B, R&D Systems), was used to assess cross-reactivity.
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bioRxiv - Molecular Biology 2022Quote: Human recombinant BMP-2 and BMP-4 were purchase from R&D Systems (Minneapolis, MN). Anti-Myc and anti-HA antibodies were purchased from Cell Signaling Technology (Danvers ...
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bioRxiv - Immunology 2020Quote: T or B cells (2-4 × 107) obtained from 2 spleens of actin-GFP mice by negative MACS kits (Thermo Fisher Scientific, 114.13D; R&D Systems, MAGM204) were intravenously injected to an actin-DsRed mouse ...
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bioRxiv - Microbiology 2021Quote: ... Cells were stained 1h at RT with a goat polyclonal anti-human ACE2 (AF933, R&D Systems) diluted at 3μg/ml in PBS + 0.3% triton X-100 + 1% horse serum + 1% BSA + 0,01% sodium azide ...
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bioRxiv - Immunology 2024Quote: ... samples were pre-treated for 1h at 37°C with 0.1 mM TAPI-2104 (R&D Systems) prior to subsequent incubation with gp33 peptide as detailed above in the continuous presence of TAPI-2 ...
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bioRxiv - Developmental Biology 2020Quote: ... or Tgfb1/2/3 ligands (10ng/ml, each) (R&D systems, 240-B/302-B2/243-B3) was placed in the upper and lower chambers ...
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bioRxiv - Developmental Biology 2021Quote: ... The cells were treated for 2 days (days 6, 7) with 50 ng/ml sonic hedgehog (SHH; R&D Systems), 2 μM dorsomorphin (DSM ...
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bioRxiv - Developmental Biology 2021Quote: ... and then further differentiated on days 4–6 with CDM3 base media supplemented with Activin A (10 ng/mL; R&D Systems), BMP4 (30 ng/mL ...
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bioRxiv - Neuroscience 2019Quote: ... IL-6 concentrations in maternal blood plasma (3 hours post-injection) were determined using a rat-specific ELISA DuoSet (R&D Systems, Abingdon, UK) as reported elsewhere [27] ...
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bioRxiv - Neuroscience 2020Quote: ... cells were treated for 1h at 37°C with 5nM of human SEMA3A Fc chimera (R&D System), dissolved in 1x Dulbecco’s PBS (DPBS ...
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bioRxiv - Epidemiology 2019Quote: ... Interleukin-6 (IL-6) was measured by ELISA (R&D systems, Abingdon, UK) and available at nine years ...
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bioRxiv - Microbiology 2022Quote: ... n = 4 each) were stained with mouse anti-SARS-CoV-2 N monoclonal antibody (R&D systems, Clone 1035111 ...
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bioRxiv - Cell Biology 2022Quote: ... CXCL1 levels were measured after 3 h and 6 h using the Human CXCL1/GROα DuoSet® ELISA kit (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: Recombinant soluble human interleukin 6 receptor (IL-6r) and recombinant human IL-6 (IL-6) were obtained from R&D systems (Minneapolis, MN). We have previously shown that these IL-6 signaling components produce equivalent effects in hyperalgesic priming (Paige et al. ...
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bioRxiv - Immunology 2020Quote: ... interleukin 6 (IL-6) and IL-1β were quantified by ELISA (R&D Systems) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... Definitive endoderm differentiation commenced 3-4 days post-passage onto fresh MEFs using 25 ng/ ml WNT3a (R&D Systems, UK) and 100 ng/ ml Activin-A (Peprotech ...
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bioRxiv - Cell Biology 2022Quote: Confluent T24 cells transfected with control and pacsin 2 RNAi in 6-well plates were treated with 10 ng/ml of recombinant human EGF (236-EG, R&D systems) in RPMI-1640 medium and incubated at 37℃ ...
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bioRxiv - Bioengineering 2022Quote: ... α6 (MAB13501; R&D systems), α3 (MAB1345 ...
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bioRxiv - Immunology 2022Quote: ... plated at 1-2×104 cells/well in 96 U-bottom plate and cultured for 3 – 6 days in cRPMI medium in the presence of IL-33 (R&D Systems, #3626, 50 ng/ml) or TSLP (R&D Systems ...
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bioRxiv - Molecular Biology 2021Quote: IL-6 secretion was measured using human IL-6 immunoassay kit (R&D Systems, D6050) as per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Interleukin-6 was measured by Human IL-6 DuoSet ELISA (DY206, R&D Systems, USA). Reagents for interleukin-10 analysis were ...
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Kinetics Of Interferon-λ And Receptor Expression In Response To In Vitro Respiratory Viral InfectionbioRxiv - Immunology 2021Quote: ... IL-6 was measured by human IL-6 DuoSet ELISA (DY206, R&D Systems, USA). Measurement of IL-10 was performed using ...
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bioRxiv - Bioengineering 2022Quote: Transduced/Transfected and sorted hGICs harbouring either Lentivirus-MGT#1 or PiggyBac-MGT#1/4 were seeded as single cells into 6-well plates in RHB-A medium supplemented with 10ng/ml TNFa (R&D Systems, 210-TA-020) or without TNFa as control ...
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bioRxiv - Cancer Biology 2020Quote: ... pH6.0 at 95°C followed by 1h blocking and incubation with pre-optimized primary anti-UCHL1 (MAB6007, R&D Systems) or anti-p53 (Dako ...
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bioRxiv - Cancer Biology 2021Quote: ... or 1h at 37°C with the sheep polyclonal anti-TWIST2 (1/200, AF6249, R&D Systems, Minneapolis, MN, USA) in Antibody Diluent Buffer from Ventana Medical Systems ...
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bioRxiv - Genetics 2021Quote: ... 3 ng/ml mouse IL-3 (R&D Systems) and 20% L-cell conditioned medium ...
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bioRxiv - Neuroscience 2023Quote: ... ectodermal paired box 6 and SRY-box transcription factor 2 (PAX6/SOX2, Biolegend, San Diego, CA, USA / R&D Systems, Minneapolis, MN, USA), and forkhead box protein A2 (FOXA2 ...
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bioRxiv - Molecular Biology 2024Quote: ... Resting or ADP (10 μM)-stimulated platelets (4 × 103/μL) were pretreated with the inhibitor hDcR3 (10 μg/mL, recombinant human decoy receptor 3 protein, R&D Systems) for 10 min and then allowed to adhere to the coated coverslips for 60 min at RT under normoxic or hypoxic conditions ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were passaged by dissociation in 0.5 mM EDTA every 3-4 days and resuspended in Essential 8 Medium supplemented with Y-27632 dihydrochloride (10 μM; Cat.#1254; R&D Systems). For CRISPR-Cas9 editing ...