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Citations for R&D Systems :
4351 - 4400 of 4586 citations for Cow Polypyrimidine tract binding protein 1 PTBP1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... resuspended in SDS loading buffer and subjected to Western Blot against phosphorylated Tyrosine (1:1000, BAM1676, R&D systems, Mineapolis, USA) or Traf6 antibody (1:2000 ...
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bioRxiv - Biochemistry 2022Quote: ... The cell layers were incubated for 1 hour with 10 µg per ml monoclonal anti-His6-tag antibody (R&D Systems) or a 1:1000 dilution of pAb 9543 279 anti-fibrillin-1 ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were incubated for 1 hour at RT with anti-PAR/pADPr monoclonal antibody (R&D Systems cat# 4335-MC-100) diluted 1:250 in 3% BSA-PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... United States) or equine TNF-α (1 ng/mL; catalog no. 1814-ET-025/CF, R&D Systems, Minneapolis, United States) in combination with LPS ...
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bioRxiv - Cell Biology 2023Quote: ... Chicken anti pan-neurofascin (against residues 25-1031 of rat neurofascin, 1:400 for IF) was from R&D System (#AF3235). Rabbit polyclonal anti-clathrin heavy chain (against residues 1650 to C-terminus of human clathrin heavy chain ...
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bioRxiv - Cancer Biology 2024Quote: ... blocking in 10% donkey serum was followed by overnight incubation at 4°C with anti-CD31 (1:100; R&D Systems) and subsequent incubation with a donkey anti-goat AlexFluor594 secondary antibody (1:500 ...
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bioRxiv - Microbiology 2024Quote: ... The cell-free supernatants were incubated with 1 mg/ml of purified recombinant human complement component C5a (R&D Systems, USA) at 37°C for 2 h ...
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bioRxiv - Neuroscience 2024Quote: ... and reseeded on laminin-521 coated plates at a split ratio of 1:2.5 in CPN medium supplemented with 20 ng/mL fibroblast growth factor 2 (FGF2; R&D Systems) and 10 µM Y-27632 ...
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bioRxiv - Cancer Biology 2021Quote: ... melanocytes were suspended in 1 ml medium and seeded on 0.5% collagen (Cultrex, R&D Systems, Minneapolis, MN, Cat # 3442-050-01), in 24 well plates for three days.
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bioRxiv - Cell Biology 2020Quote: ... mOSE cells (1×10^6) were first plated as monolayer cultures 24 hr prior to treatment with TGFB1 (10 ng/mL, R&D Systems). Four days after the addition of TGFB1 ...
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bioRxiv - Cell Biology 2020Quote: ... Once the cells were adherent the cells undergoing differentiation were cultured in basal medium supplemented with 1% v/v Adipogenic Supplement (R&D Systems) while the control cells not undergoing differentiation were cultured in basal medium ...
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bioRxiv - Cell Biology 2020Quote: ... sections were incubated over night at 4 °C with the following antibodies diluted in PBS 2.5% BSA: goat anti-CD140a 1:80 (R&D Systems AF1062) and rabbit anti-PPAR-gamma 1:200 (Cell Signaling 2443S) ...
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bioRxiv - Immunology 2021Quote: ... The immunoblots were probed overnight at 4 °C or 1 hour at room temperature with anti-human IL-1β antibody (AF-201-NA, R&D Systems); anti-human Caspase-1 antibody (06-503 ...
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bioRxiv - Developmental Biology 2021Quote: ... micropattern cultures were fed in lung induction medium (LIM) which contains cSFDM supplemented with 50 ng*mL−1 KGF (or indicated in each experiment; R&D systems), 10 ng*mL−1 BMP4 (R&D systems) ...
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bioRxiv - Bioengineering 2022Quote: ... jam-packed microgels in the pellet were mixed with collagen solution (5 mg mL-1 Cultrex rat collagen I, R&D Systems) in 2:1 ratio ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies used for immunofluorescence in this study were as follows: anti-mouse 1:150 GPR34 (R&D Systems; Cat. No. MAB4617), anti-rabbit 1:1000 IBA1 (Wako ...
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bioRxiv - Molecular Biology 2021Quote: Retinal organoids on day 90 of differentiation were incubated at RT for 1 hour in PBS with either a goat anti-ACE2 antibody (AF933, R&D Systems) or a normal goat IgG control (AB-108-C ...
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bioRxiv - Immunology 2019Quote: ... and resuspended for culture at 37°C + 5% CO2 for 1 h prior to stimulation with recombinant human TNFα (R&D Systems). Following stimulation ...
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bioRxiv - Immunology 2019Quote: ... 96-well flat bottom plates were pre-coated with 20 μg/ml of an agonist anti-TREM-1 mAb (clone 174031; R&D Systems) overnight at 37°C ...
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bioRxiv - Immunology 2019Quote: ... a total of 1 × 106 RAW264.7 or primary peritoneal macrophages were washed with FACS buffer containing PBS with 2% FBS and stained with APC anti-mouse TREM-1 Ab (clone: 174021, R&D systems). Unstained cells were used as a negative control to establish the flow cytometer voltage setting ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... pH 7.6] for 2 h at RT and incubated overnight (ON) at 4°C with antibodies against TRKB (1:1000, #AF1494, R&D Systems); AP2M (1:1000 ...
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bioRxiv - Immunology 2019Quote: ... IL-17 was detected using goat anti-mouse IL-17 (1/100 dilution, Catalogue number Af-421-na R&D Systems, UK) followed by biotinylated rabbit anti-goat IgG (1/200 dilution ...
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bioRxiv - Molecular Biology 2019Quote: ... of LPS treated rats were double-immunostained for the analysis of the cellular localization of cathepsin X using the following primary antibodies: goat polyclonal anti-cathepsin X antibody (1:75, R&D System), mouse monoclonal anti-TH antibody (1:500 ...
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bioRxiv - Immunology 2021Quote: ... Slides were incubated with a primary antibody cocktail containing one or more of the following antibodies overnight at 4°C: goat anti-mouse NKp46 (1:100, R&D Systems), anti-mouse CD3e-AF594 or AF647 (500A2 ...
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bioRxiv - Microbiology 2021Quote: ... Cells were stimulated for 16 h prior to infection with addition of 50 IU mL-1 human IFNγ (285-IF, R&D Systems). Induction of GBP expression in the Dox-inducible cells was performed with 200 ng mL-1 Dox overnight (D9891 ...
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bioRxiv - Bioengineering 2020Quote: ... The slides were fixed in an ice-cold acetone/methanol mixture (50%/50%) and stained with primary anti-mouse CD144 antibody (MAB9381, 1:100, R&D Systems) and secondary donkey-anti-mouse Alexa Fluor® 594 (A21203 ...
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bioRxiv - Bioengineering 2020Quote: ... supplemented with 1 ng/mL transforming growth factor beta-1 (TGF-β1) and 5 ng/mL fibroblast growth factor-2 (FGF-2) (both from R&D Systems) at 37°C and 5% CO2 in a humidified incubator (Thermo Scientific Heraeus ...
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bioRxiv - Immunology 2021Quote: ... Cell supernatant was collected and 1 ml was used for Proteome Profiler™ Human Cytokine Array (ARY005B, R&D Systems, Inc., USA) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... Brain sections were blocked with 5% normal donkey serum followed by overnight incubation at 4 °C using the following mixture of primary antibodies: goat anti-IL-1β/ILF2 (1:250; #AF-401-NA, R&D Systems), rat anti-CD45 (1:250 ...
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bioRxiv - Microbiology 2020Quote: ... cells were wash three times with PBS to remove any residual FBS and followed by 1-hour treatment with goat anti-human ACE2 antibody (R&D Systems) or anti-hDDP4 antibody (R&D Systems ...
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bioRxiv - Systems Biology 2021Quote: ... Cells were incubated overnight in myogenic growth media (F10, 20% FBS, 1% Pen/Strep; Gibco, supplemented with 5 ng/mL rFGF2; R&D Systems).
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bioRxiv - Immunology 2020Quote: ... The nitrocellulose membrane was blocked in 5 % non-fat milk in PBS-T for 2 hours at room temperature and probed overnight with goat anti-hACE2 primary Ab at 1 μg/mL (AF933, R&D systems). Following three wash intervals of 10 minutes with PBS-T ...
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bioRxiv - Biophysics 2022Quote: Endogenous CXCR4 expression on the surface of HEK and U87 cells was monitored by flow cytometry using CXCR4-specific phycoerythrin-conjugated mAb 12G5 or the corresponding isotype control (1:20, R&D Systems) in a BD FACS LSR Fortessa cytometer (BD Biosciences) ...
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bioRxiv - Microbiology 2022Quote: ... followed by three days incubation at 37 °C in the presence of 1 µg/ml of kifunensine (R&D Systems, MN, USA). Supernatant was collected and combined with half volumes of the supernatant of binding buffer (25 mM HEPES ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were fed daily with different cocktails of growth factors and small molecules for twelve days in mesodermal differentiation medium and driven through the anterior primitive streak (1 day; 30 ng/ml Activin [cat. num. 338-AC; R&D Systems, Minneapolis ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were kept in serum-free medium for 4 hours prior to stimulation and then incubated with recombinant CSF-1 or IL-34 (R&D Systems) for the indicated time points ...
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bioRxiv - Cell Biology 2021Quote: ... sections were blocked with 10% rabbit serum for 15min at room temperature and then incubated with goat anti mouse IGF1(1:40, AF791, R&D System) overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: ... Cells were treated at 37 °C for 30 minutes with 15 μm Forskolin and 1:3 serial dilutions of potential ligands with the following starting concentrations: 10 μM recombinant human CXCL17 (R&D Systems), 10 μM lysophosphatidic acid (LPA ...
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bioRxiv - Molecular Biology 2020Quote: Randomly selected sections coming from ovarian tissue of least 3 different individuals were used for immunohistochemistry for Anti-active Caspase-3 (AC-3, 1:1000, AF-835, R&D Systems), γH2AX (1:250 IHC-00059 ...
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bioRxiv - Biochemistry 2020Quote: ... Jurkat E6.1 cell lines were incubated with 5 µL of 25 µg/mL anti-hCD3ε antibody (PE Conjugated Mouse IgG1, R&D System) or 2 µL of 200 µg/mL isotype control (PE Mouse IgG1 ...
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bioRxiv - Cancer Biology 2021Quote: ... Fibroblasts+rhTGFβ1 medium was incubated with 1 μg/mL of anti-human HGF antibody or with the respective mouse IgG1 isotype control (both from R&D Systems) for 1 hour at 37ºC.
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bioRxiv - Developmental Biology 2019Quote: ... and 100 ng/ml CHO-derived mouse R-spondin 1 or 5 ng/ml CHO-derived mouse R-spondin 2 (R&D System). The final concentration of Rspondins in the culture medium was initially tested in pilot experiments in order to add comparable amounts of bioactive ligands (bioactivity measured by TOPflash assays and organoid growth curves ...
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bioRxiv - Bioengineering 2019Quote: ... Primary antibodies (1:300 dilutions of rat-anti-C-peptide (DSHB; GN-ID4-S) and goat anti-PDX1 (R&D Systems; AF2419) were left on overnight ...
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bioRxiv - Cell Biology 2019Quote: ... Incubation with primary rat-polyclonal antibody against LYVE-1 in combination with rat-polyclonal biotinylated anti-MHC-II antibody (both R&D Systems) was done for 2h at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were blocked in 5% milk in 0.2% Tween-20 in TBS for 1h at room temperature and probed with primary antibodies against SORCS2 (1:750; AF4238, R&D Systems) and GAPDH (1:10,000 ...
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bioRxiv - Developmental Biology 2021Quote: ... live cultures were incubated for 1h in a cell culture incubator with culture medium containing 1:500 anti-PODXL antibody (MAB1556, R&D Systems). The cells and BME drops were subsequently fixed with 4% PFA ...
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bioRxiv - Cancer Biology 2020Quote: ... After three PBST washes the dot blots were incubated with high sensitivity streptavidin conjugated to horseradish peroxidase (1:10 dilution, 865006, R&D Systems) for 90 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... Dot blots were washed with PBST three times and incubated with 30 μl of biotinylated anti-rabbit secondary antibody for 90 minutes (1:10 dilution, 865002, R&D Systems). After three PBST washes the dot blots were incubated with high sensitivity streptavidin conjugated to horseradish peroxidase (1:10 dilution ...
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bioRxiv - Systems Biology 2019Quote: ... cells were washed with 1× Phosphate buffered saline (PBS) and stimulated with 1000 U/mL of human recombinant IFNβ (R&D Systems) in DMEM for the designated length of time ...
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bioRxiv - Immunology 2020Quote: ... Cells were re-seeded onto 24-well plates at 1 × 105 cells/well and polarized for 48 hr with the addition of 50 ng/ml human IFNγ (R&D systems) for M1 macrophages ...