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Citations for R&D Systems :
2651 - 2700 of 4069 citations for 3 Methoxy 2 2 4 4 Tetrabde Unlabeled 50 Ug Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... and TNF levels were determined in the supernatants of Calu-3 and macrophage cultures using ELISA kits following the manufacturer’s instructions (R&D Systems). HMGB-1 was determined in the cell-free TA supernatants and in the supernatants of Calu-3 cultures using immunoassay following the manufacturer’s instructions (Novus Biologicals) ...
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bioRxiv - Neuroscience 2022Quote: ... The plates were washed 3 times with washing buffer and a horseradish peroxidase-streptavidin (HRP-Strep) complex (R&D Systems DY998) diluted 1:25 in reagent diluent was added to the plates and incubated for 40 minutes with agitation ...
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bioRxiv - Molecular Biology 2022Quote: ... Subsequently plates were washed 3 times with PBS-T and primary antibody (anti-RBD-mouse, R&D systems, Minneapolis, MN, USA) was added in a dilution of 1 ...
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bioRxiv - Bioengineering 2023Quote: OCI-AML5 cells were cultured in 24-well plates (3×105 cells/well) in cRPMI media (FBS from R&D Systems; Minneapolis ...
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bioRxiv - Developmental Biology 2019Quote: ... Dry pellets were re-dissolved in 50 μl RIPA buffer and IGF2 measured by ELISA (Mouse IGF-II DuoSet ELISA kit, R&D Systems – DY792) using an assay adapted for the MesoScale Discovery electrochemiluminescence immunoassay platform (MSD) ...
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bioRxiv - Cell Biology 2022Quote: ... 100μg of GST-NEDD4△52 was bound to 10 μl Glutathione sepharose resin and incubated with 50 nM ubiquitin activating enzyme UBA1 (R&D Systems, E305025), 750 nM ubiquitin conjugating enzyme UBCH5B (R&D Systems ...
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bioRxiv - Physiology 2022Quote: ... or immunofluorescent (IF) staining as indicated below: Antibody against VE-cadherin extracellular domain (WB 1:1000, IF 1:50, R&D Systems, MAB9381), antibody against VE-cadherin intracellular domain (WB 1:1000 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 100 µl) in N2-S medium supplemented with 20 ng/ml EGF and 20 ng/ml FGF2 (both from R&D Systems, Minneapolis, Minnesota, USA) into the upper chamber ...
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bioRxiv - Developmental Biology 2020Quote: ... was coated with 200 µL 91 µg/mL Matrigel in PBS or 8 µg/mL E-cadherin extracellular domain (E-cad-ECD; R&D Systems, 8505-EC-050) in PBS for 1-3 hours at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... The neural medium was supplemented with 10 ng/mL epidermal growth factor (PreproTech, AF-100-15) and 10 ng/mL basic fibroblast growth factor (R&D Systems, 234-FSE-025) and changed daily until day 12 ...
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bioRxiv - Cancer Biology 2020Quote: ... Invasion was also quantified in response to serum-free DMEM supplemented with 10 ng/mL of recombinant mouse CSF2 (R&D Systems, 415-ML-5), PBS or conditioned media from lung stromal cells from HFD-fed mice treated with 5 μg/mL of either CSF2 neutralizing antibodies (R&D Systems ...
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bioRxiv - Immunology 2021Quote: ... 50 ng/mL of recombinant human GM-CSF (Sanofi-aventis Cat# NAC2004–5843-01) and 2.5 ng/mL of tumor necrosis factor (TNF)-α (25 ng/mL; R&D Systems Cat# 210-TA). Next ...
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bioRxiv - Molecular Biology 2022Quote: ... The neural medium was supplemented with 10 ng/mL epidermal growth factor (PreproTech AF-100-15) and 10 ng/mL basic fibroblast growth factor (R&D Systems 234-FSE-025). From day 6 to day 10 the medium was changed everyday until the appearance of rosettes ...
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bioRxiv - Developmental Biology 2022Quote: ... the media was changed with full media containing FGF (10 ng/ml bFGF, Sigma Aldrich #F0291; 25 ng/ml rhFGF, R&D Systems #345-FG), BMP-4 (40 ng/ml ...
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bioRxiv - Cell Biology 2022Quote: ... BMMs were then seeded at 1 × 106 cells/well in 24-well plates and stimulated with 30 ng/mL RANKL and 30 ng/mL M-CSF (R&D Systems, Minneapolis, MN, USA) for 5 days to generate mature OCs ...
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bioRxiv - Immunology 2024Quote: ... cells were plated at 1 x 106 cells per well in round bottom 96 well plates and stimulated with 100 ng/mL recombinant porcine IL-12p70 and 100 ng/mL recombinant porcine IL-18 (both from R&D Systems; Minneapolis, MN, USA) for 13 hours at 37 °C and 5% CO2 in the presence of Brefeldin A (3 µg/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... BMSCs were plated in T-75 flasks overnight with standard OCY454 media supplemented with 10ng/ml M-CSF (R&D Systems Catalog #416-ML-010) at 37°C in 5% CO2 ...
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bioRxiv - Immunology 2023Quote: ... the cells were plated at 0.2M cells/ml in a 6-well plate and stimulated with IFNβ (500 IU/ml, R&D systems cat. 8499-IF-010). At 24h ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 10% FBS and 1% penicillin/streptomycin with macrophage-colony stimulating factor (M-CSF, 30 ng/mL; 416-ML, R&D systems, Minneapolis, MN, USA) for 4 days to obtain BMMs ...
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bioRxiv - Cell Biology 2023Quote: ... osteoclast differentiation was initiated using αMEM complete media supplemented with 30 ng/mL M-CSF and 100 ng/mL RANKL (R&D Systems, 462-TEC/CF)(57) ...
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bioRxiv - Microbiology 2021Quote: ... Serum concentrations of IGF-1 and IGFBP-3 were determined using the Mouse/Rat IGF-1 Quantikine ELISA kit (R&D Systems) and IGFBP-3 DuoSet ELISA kit (R&D Systems) ...
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MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: ... Next day the plates were washed 3 times and the color development was performed using Duoset ELISA Development System (DY999, R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... supernatants were collected and secretion of TGF β and MMP2/3/9 was assessed in the supernatant by TGFβ1 ELISA kit (R&D systems) or MMP2/3/9 luminex kit (eBiosciences ...
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bioRxiv - Bioengineering 2020Quote: Lambda interferon levels were evaluated by human IL-29/IL-28B (IFN-lambda 1/3) DuoSet ELISA (DY1598B, R&D Systems, USA). Biological activity experiments were performed using ...
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bioRxiv - Bioengineering 2020Quote: ... The relative expression levels of apoptosis related proteins of post I/RI iCMs (n=3) were analyzed using the Proteome Profiler Human Apoptosis Array (ARY009, R&D Systems), according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: The relative expression levels of cellular stress related proteins of iCMs (n=3) were analyzed using the Proteome Profiler Human Cell Stress Array (ARY018, R&D Systems), according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: The relative cytokine content of the decellularized mouse heart tissues (n=3) were obtained using the Mouse XL Cytokine Array Kit (ARY028, R&D Systems). Briefly ...
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bioRxiv - Immunology 2020Quote: ... Cells were treated at 37 °C for 30 minutes with 15 μm Forskolin and 1:3 serial dilutions of potential ligands with the following starting concentrations: 10 μM recombinant human CXCL17 (R&D Systems), 10 μM lysophosphatidic acid (LPA ...
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bioRxiv - Microbiology 2019Quote: ... Cell supernatants were collected and levels of IFNλ were determined with the IFN-lambda 1/3 DuoSet ELISA kit (R&D Systems). Plates were read on a Synergy HT plate reader (Biotek ...
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Kinetics Of Interferon-λ And Receptor Expression In Response To In Vitro Respiratory Viral InfectionbioRxiv - Immunology 2021Quote: ... Lambda IFN levels were evaluated by human IL-29/IL-28B (IFN-lambda 1/3) DuoSet ELISA (DY1598B, R&D Systems, USA). IL-6 was measured by human IL-6 DuoSet ELISA (DY206 ...
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bioRxiv - Biochemistry 2020Quote: ... and furin (3 nM) in the presence of the fluorogenic substrate Mca-KPLGL-Dpa-AR-NH2 (Catalog no. ES010, R&D systems). The final substrate concentration was 10 μM in 50 mM Tris ...
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bioRxiv - Immunology 2021Quote: ... We used 3 different kits to quantify sCD25 by Human CD25/IL-2R alpha Quantikine ELISA Kit (R&D System, Cat# DR2A00), IP-10 by Human IP- 10 ELISA Kit (CXCL10 ...
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bioRxiv - Cell Biology 2022Quote: ... Rabbit anti-ALIX and rabbit anti-CHMP4B antibodies were described previously (Christ et al, 2016). Human Galectin-3 Alexa Fluor 488-conjugated antibody (cat. no. IC1154G) was purchased from R&D Systems. Mouse anti-LAMP1 (cat ...
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bioRxiv - Microbiology 2023Quote: ... Human IL-29/IL-28B (IFN-lambda 1/3) DuoSet ELISA or Human CXCL10/IP-10 DuoSet ELISA reagents (biotechne R&D systems) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2023Quote: ... 0.1 million cells (in 100 µL of 1:1 mix of PBS and Cultrex Basement Membrane Extract (Type 3, R&D systems)) were injected into the 4th mammary fat pad of 7-8 weeks old female BALB/c mice ...
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bioRxiv - Immunology 2023Quote: ... centrifuged at 4°C for 5 min at 5000 g and middle layers collected and stored at −80°C until analysis using Human CCL26/Eotaxin-3 DuoSet ELISA from R&D Systems/Bio-Techne (Cat ...
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bioRxiv - Cancer Biology 2022Quote: ... or the combination of both drugs in methylcellulose media enriched with human cytokines (recombinant human SCF, GM-CSF, G-CSF, IL-3, IL-6, Epo, #HSC005, R&D Systems). The methylcellulose mixture was plated in three replicates to 35 mm culture dishes using regular syringes with 16 gauge non-stick needles and incubated at 37°C ...
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bioRxiv - Immunology 2023Quote: ... the non-adherent fraction was seeded at 106 cells/well in 24-well plates in medium containing 30ng/ml M-CSF and 10ng/ml RANKL (R&D Systems, Cat. 452-TEC-019). The medium was replaced with supplemented M-CSF and RANKL after 48hr ...
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bioRxiv - Cell Biology 2023Quote: ... after which the medium was changed to α-MEM containing 20 ng/ml M-CSF and 20 ng/ml RANKL (462-TEC; R&D Systems, Minneapolis, MN, USA). The differentiation medium was changed every 24 hr during the 5-day differentiation period.
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bioRxiv - Genomics 2024Quote: ... 3 units/mL EPO (Amgen, EPOGEN NDC 55513-144-01), 200 μg/mL holo-transferrin (Millipore Sigma, T0665, 10 ng/mL recombinant human SCF (R&D systems, 255-SC/CF), and 1 ng/mL recombinant human interleukin IL-3 (R&D systems ...
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bioRxiv - Developmental Biology 2021Quote: ... and 20 ng/ml FGF2 (R&D systems, 233-FB-025) referred to from now on as NMP differentiation medium ...
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bioRxiv - Cell Biology 2021Quote: ... or HB-EGF (100 ng/ml; R&D Systems, Minneapolis, MN) as indicated in the Figure legends ...
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bioRxiv - Cancer Biology 2021Quote: ... and then supplemented with 1 µg/mL VEGF (R&D Systems), 1 µg/mL bFGF (R&D Systems ...
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bioRxiv - Neuroscience 2020Quote: ... and 20 ng/ml FGF2 (R&D Systems, 233-FB- 01M) in CDM2 basal media ...
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bioRxiv - Cell Biology 2020Quote: ... Treatment conditions included IL-13 (20 ng/ml, R&D Systems), HSP90 inhibitor geldanamycin (25 μM ...
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bioRxiv - Developmental Biology 2021Quote: ... 100 ng/ml recombinant FLT1-FC (R&D Systems, 7756-FL), 1 µg/ml anti-FLT1 monoclonal antibody (Angio-Proteomie ...
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bioRxiv - Immunology 2021Quote: ... IFNλ1 (100 ng/mL; 1598-IL-025, R&D Systems, USA); and Ruxolitinib (10 µM ...
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bioRxiv - Immunology 2021Quote: ... 1 µg/ml recombinant murine CD40L (CD40L, R&D Systems; Biotechne) or 10 µg/ml LPS from Salmonella minnesota R595 (LPS ...
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bioRxiv - Developmental Biology 2021Quote: ... or anti-FST antibody (20 μ/ml, AF669, R&D Systems) for 42 hr ...
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bioRxiv - Developmental Biology 2021Quote: ... 500 ng/ml EGF (R&D Systems, cat. 2028-EG-010) and 1000 U/ml ESGRO (Millipore ...