Labshake search

2401 - 2450 of 10000+ citations since 2019

• Clonal and Scalable Endothelial Progenitor Cell Lines from Human Pluripotent Stem Cells

  Jieun Lee, et al., bioRxiv - Cell Biology 2023

  Quote: ... RFP labeled eEPCs were plated with monolayer and 10ng/ml of recombinant human VEGF protein (R&D System, Cat# 293-VE-010) was treated to enhance tube formation ...
• KAT6A mutations drive transcriptional dysregulation of cell cycle and Autism risk genes in an Arboleda-Tham Syndrome cerebral organoid model

  Aileen A. Nava, et al., bioRxiv - Genetics 2023

  Quote: ... recombinant human EGF at 20 ng/mL (R&D systems #236-EG-200), and recombinant human FGF2 at 20 ng/uL (R&D systems #233-FB-010).
• Selective ablation of thymic and peripheral Foxp3⁺ regulatory T cell development

  Acelya Yilmazer, et al., bioRxiv - Immunology 2023

  Quote: ... Abs to Nrp1 (polyclonal goat IgG-AF700) were purchased from R&D Systems. Intracellular expression of cytokines and transcription factors was analyzed using the respective fluorochrome-coupled mAbs in conjunction with either the BD Cytofix/Cytoperm kit (BD ...
• KAT6A mutations drive transcriptional dysregulation of cell cycle and Autism risk genes in an Arboleda-Tham Syndrome cerebral organoid model

  Aileen A. Nava, et al., bioRxiv - Genetics 2023

  Quote: ... and recombinant human FGF2 at 20 ng/uL (R&D systems #233-FB-010).
• Patterning and folding of intestinal villi by active mesenchymal dewetting

  Tyler R. Huycke, et al., bioRxiv - Developmental Biology 2023

  Quote: E13.5 small intestinal tissues were harvested in Calcium/Magnesium free (CMF) PBS and washed several times in CMF PBS before being placed in 3mL of Cultrex Organoid Harvesting Solution (R&D Systems), a non-enzymatic basement membrane depolymerization solution ...
• The super-healing MRL strain promotes muscle growth in muscular dystrophy through a regenerative extracellular matrix

  Joseph G. O’Brien, et al., bioRxiv - Cell Biology 2023

  Quote: ... and Mouse IGF-1 Quantikine ELISA kit (R&D Systems, MG100), respectively ...
• A Novel Humanized Immune Stroma PDX Cancer Model for Therapeutic Studies

  Dongli Yang, et al., bioRxiv - Cancer Biology 2023

  Quote: ... or 50 ng/mL and IL4 and IL13 (R&D Systems) for 48 hours ...
• A Novel Humanized Immune Stroma PDX Cancer Model for Therapeutic Studies

  Dongli Yang, et al., bioRxiv - Cancer Biology 2023

  Quote: ... supplemented with 50 ng/mL human GM-CSF (R&D Systems) to stimulate macrophage differentiation ...
• Border-forming wound repair astrocytes

  Timothy M. O’Shea, et al., bioRxiv - Neuroscience 2023

  Quote: ... goat anti-CD13 (1:600, AF2335; R&D Systems, USA); rat anti-Cd44 (1:400 ...
• Border-forming wound repair astrocytes

  Timothy M. O’Shea, et al., bioRxiv - Neuroscience 2023

  Quote: Primary antibodies goat anti-A2m (1:300, AF1938; R&D Systems); rabbit anti-Aldh1l1 (1:1000 ...
• Single tyrosine mutation in VE-cadherin modulates gene lung expressions: evidence for FOXF1 mediated S1PR1 upregulation to stabilize vessels in mice

  Olivia Garnier, et al., bioRxiv - Cell Biology 2023

  Quote: ... Mouse Angiogenesis array kit (ARY015, R&D system) was purchased for protein array ...
• Synthetic Frizzled agonist and LRP antagonist for high-efficiency Wnt/β-catenin signaling manipulation in organoid cultures and in vivo

  Quanhui Dai, et al., bioRxiv - Synthetic Biology 2023

  Quote: ... and 100 ng/mL TNF-α (R&D Systems) in the absence or presence of 50 ng/mL sWnt ...
• AMPK Drives Both Glycolytic and Oxidative Metabolism in T Cells During Graft-versus-host Disease

  Archana Ramgopal, et al., bioRxiv - Immunology 2023

  Quote: ... Human IFNγ levels were detected via enzyme linked immunosorbent assay (ELISA) using the Human IFNγ Quantikine ELISA kit (R&D Systems) per manufacturer’s instructions ...
• p97/VCP targets Toxoplasma gondii vacuoles for parasite restriction in interferon-stimulated human cells

  Barbara Clough, et al., bioRxiv - Immunology 2023

  Quote: Cells were stimulated for 18-24h in complete medium at 37˚C with addition of 50- 100U/ml human IFNγ (R&D Systems #285-IF-100).
• Interleukin-27-induced HIV-resistant dendritic cells suppress reveres transcription following virus entry in an SPTBN1, Autophagy, and YB-1 independent manner

  Tomozumi Imamichi, et al., bioRxiv - Immunology 2023

  Quote: ... recombinant IL-27 (R&D systems) was added to the G4 media during dendritic cell differentiation at a concentration of 100 ng/ml ...
• Modeling kidney development, disease, and plasticity with clonal expandable nephron progenitor cells and nephron organoids

  Biao Huang, et al., bioRxiv - Developmental Biology 2023

  Quote: ... (Note, for coating with Matrigel (R&D Systems, # 3433-010-01) in one well of 96-well plate ...
• Modeling kidney development, disease, and plasticity with clonal expandable nephron progenitor cells and nephron organoids

  Biao Huang, et al., bioRxiv - Developmental Biology 2023

  Quote: ... and ITGA8 antibody (R&D Systems, # AF4076) was added at the ratio of 1:200 for live cell staining ...
• Periodontitis promotes bacterial extracellular vesicle-induced neuroinflammation in the brain and trigeminal ganglion

  Jae Yeong Ha, et al., bioRxiv - Pathology 2023

  Quote: ... anti–IL-6 (1:200, AF406NA; R&D Systems), anti-GFAP (1:400 ...
• Air Pollution Aggravates Renal Ischemia-Reperfusion-Induced Acute Kidney Injury

  Talita Rojas Sanches, et al., bioRxiv - Pathology 2023

  Quote: Urinary neutrophil gelatinase-associated lipocalin (NGAL) was measured by using a commercially available ELISA kit (R&D Systems), according to the instructions provided by the manufacturer ...
• Personalized models of breast cancer desmoplasia reveal biomechanical determinants of drug penetration

  Giovanni S. Offeddu, et al., bioRxiv - Bioengineering 2023

  Quote: ... CCL2 (#279-MC-010, R&D Systems), CCL4 (#271-BME-010 ...
• Personalized models of breast cancer desmoplasia reveal biomechanical determinants of drug penetration

  Giovanni S. Offeddu, et al., bioRxiv - Bioengineering 2023

  Quote: ... TNFα (#210-TA-005, R&D Systems), CCL2 (#279-MC-010 ...
• Personalized models of breast cancer desmoplasia reveal biomechanical determinants of drug penetration

  Giovanni S. Offeddu, et al., bioRxiv - Bioengineering 2023

  Quote: ... and Cetuximab (#MAB9577, R&D Systems) were co-perfused at a concentration of 20 µg mL-1 ...
• Personalized models of breast cancer desmoplasia reveal biomechanical determinants of drug penetration

  Giovanni S. Offeddu, et al., bioRxiv - Bioengineering 2023

  Quote: ... iii) Enzyme linked immunosorbent assay (ELISA) of HA (#DHYAL0, R&D Systems), normalized to total protein content ...
• Personalized models of breast cancer desmoplasia reveal biomechanical determinants of drug penetration

  Giovanni S. Offeddu, et al., bioRxiv - Bioengineering 2023

  Quote: ... and a TGFβ blocking antibody (20 µg mL-1, #MAB1835, R&D Systems). Trastuzumab (#MAB9589 ...
• Personalized models of breast cancer desmoplasia reveal biomechanical determinants of drug penetration

  Giovanni S. Offeddu, et al., bioRxiv - Bioengineering 2023

  Quote: ... TGFβ (#MAB1835, R&D Systems); iii ...
• CYTOTOXIC ACTIVITY OF A NEW ISOFORM L-AMINO ACID OXIDASE (BALT-LAAO-II) FROM Bothrops alternatus (URUTU) SNAKE VENOM IN HUMAN LEUKEMIC HL60 CELLS

  Maurício Aurelio Gomes Heleno, et al., bioRxiv - Pharmacology and Toxicology 2023

  Quote: ... Annexin V/propidium iodide apoptosis detection kits were obtained from R&D Systems Inc ...
• Contribution of bacterial and host factors to pathogen “blooming” in a gnotobiotic mouse model for Salmonella enterica serovar Typhimurium-induced enterocolitis

  Markus Beutler, et al., bioRxiv - Microbiology 2023

  Quote: ... Neutrophil specific elastase was quantified using the Mouse Neutrophil Elastase/ELA2 DuoSet ELISA kit and the DuoSet Ancillary Reagent Kit2 (both from R&D Systems). Cecal pathology was scored at necropsy ...
• Chemokine receptor 5 signaling in PFC mediates stress susceptibility in female mice

  Hsiao-Yun Lin, et al., bioRxiv - Animal Behavior and Cognition 2023

  Quote: ... or Enzyme Linked Immunosorbent Assay (ELISA, Mouse CCL5/RANTES DuoSet ELISA, #DY478, R&D systems) according to the manufacturer’s instructions.
• GSK3α/β restrains IFNγ-inducible costimulatory molecule expression in alveolar macrophages, limiting CD4⁺ T cell activation

  Laurisa M. Ankley, et al., bioRxiv - Immunology 2023

  Quote: iBMDMs were maintained in Dulbecco’s Modified Eagle Medium (DMEM; HyClone Cytiva, Cat no. SH30243.01) supplemented with 10% fetal bovine serum (FBS) (R&D Systems, Cat no. S11550). iBMDMs were passaged once they reached 70–90% confluency ...
• Sustained store-operated calcium entry utilizing activated chromatin state leads to instability in iTregs

  Huiyun Lyu, et al., bioRxiv - Immunology 2023

  Quote: Recombinant Human IL-2 and human TGF-β were from R&D systems. Anti-mouse CD3 and biotin-anti-CD3 (145-2C11 ...
• Variable PD-1 glycosylation modulates the activity of immune checkpoint inhibitors

  Chih-Wei Chu, et al., bioRxiv - Immunology 2023

  Quote: Serum and recombinant PD-1 was quantified by human PD-1 Quantikine ELISA and human PD-1 DuoSet ELISA (R&D Systems, Minneapolis, MN), following manufacturer’s protocol ...
• Human inborn errors of long-chain fatty acid oxidation show impaired inflammatory responses to TLR4-ligand LPS

  Signe Mosegaard, et al., bioRxiv - Immunology 2023

  Quote: ... IL-6 (BioLegend® Cat. No. 430504 and R&D Systems Cat. No. DY206), IL-8 (Thermo Scientific 88-8086) ...
• Homeostatic Regulation of Seizure Susceptibility and Cognitive Function by Derlin-1 through Maintenance of Adult Neurogenesis

  Naoya Murao, et al., bioRxiv - Neuroscience 2023

  Quote: ... or bFGF (10 ng/mL) and diazepam (100 μM) (045-18901, Wako) or BMP4 (50 ng/mL) (5020-BP, R&D Systems) (quiescence medium ...
• Legionella pneumophila inhibits type I interferon signaling to avoid cell-intrinsic host cell defense.

  Charles N.S. Allen, et al., bioRxiv - Microbiology 2023

  Quote: The levels of secreted IFN-β were measured by an IFN-β enzyme-linked immunosorbent assay (ELISA) (R&D Systems) following the manufacturer’s protocol ...
• False positive glycopeptide identification via in-FAIMS fragmentation

  Valentina Rangel-Angarita, et al., bioRxiv - Biochemistry 2023

  Quote: ... transferrin (R&D systems, 2914-HT), and apolipoprotein E (MoleCular Innovations ...
• Fetal manipulation of maternal metabolism is a critical function of Igf2 imprinting

  Jorge Lopez-Tello, et al., bioRxiv - Developmental Biology 2023

  Quote: ... Slides were incubated overnight with goat anti-human IGF-II antibody (R&D Systems; AF292). Alexa Fluor® 488-AffiniPure antibody (Jackson ImmunoResearch ...
• Astroglial Hmgb1 regulates postnatal astrocyte morphogenesis and cerebrovascular maturation

  Moises Freitas-Andrade, et al., bioRxiv - Developmental Biology 2023

  Quote: ... CD31 (R&D system, AF3628), and Syto83 (ThermoFisher ...
• The salt-and-pepper pattern in mouse blastocysts is compatible with signalling beyond the nearest neighbours

  Sabine C. Fischer, et al., bioRxiv - Developmental Biology 2023

  Quote: ... anti-GATA6 (R&D Systems, 1:200) They were rinsed three time in blocking buffer for 15 minutes each and incubated in secondary antibody solution for 1 hour in the dark ...
• AT7867 promotes pancreatic progenitor differentiation of human iPSCs and accelerates diabetes reversal

  Nerea Cuesta-Gomez, et al., bioRxiv - Developmental Biology 2023

  Quote: ... PDX1 (Cat. No. AF2419, R&D System) and KI67 (Cat ...
• AT7867 promotes pancreatic progenitor differentiation of human iPSCs and accelerates diabetes reversal

  Nerea Cuesta-Gomez, et al., bioRxiv - Developmental Biology 2023

  Quote: ... and 50 ng/ml KGF (Cat. No. 251-KG-MTO, R&D System) for 2 days ...
• Transcriptional and epigenomic profiling identifies YAP signaling as a key regulator of intestinal epithelium maturation

  Laura M. Pikkupeura, et al., bioRxiv - Developmental Biology 2023

  Quote: ... Sox17 (R&D systems #AF1924, 1:300) and Gata4 (Santa Cruz #sc-25310 ...
• Microglial synaptic pruning in the nucleus accumbens during adolescence sex-specifically influences splenic immune outcomes

  J. M. Kirkland, et al., bioRxiv - Neuroscience 2023

  Quote: ... and IFNγ ELISAs were purchased from R&D Systems (#R2000 ...
• Rational screening for cooperativity in small-molecule inducers of protein–protein associations

  Shuang Liu, et al., bioRxiv - Biophysics 2023

  Quote: ... BirA (R&D systems 10464-BA, 90 μL) and HRV 3C protease (Pierce 88946 ...
• IL-1β promotes MPN disease initiation by favoring early clonal expansion of JAK2-mutant hematopoietic stem cells

  Shivam Rai, et al., bioRxiv - Cancer Biology 2023

  Quote: ... IL-1β and other pro-inflammatory cytokine levels in mouse BM and plasma were measured by ELISA kits from R&D systems and Mesoscale Discovery according to manufacturer’s instructions ...
• Loss of myeloid cannabinoid CB1 receptor confers atheroprotection by reducing macrophage proliferation and immunometabolic reprogramming

  Yong Wang, et al., bioRxiv - Immunology 2023

  Quote: ... IL1β levels in BMDM supernatants were measured by DuoSet ELISA (R&D Systems) with a Tecan Infinite F200 PRO microplate reader ...
• Lack of mTORC2 signaling in CD11c⁺ myeloid cells inhibits their migration and ameliorates experimental colitis

  Aline Ignacio, et al., bioRxiv - Immunology 2023

  Quote: ... Kits were purchased from R&D Systems (Minneapolis, MN, USA) and performed as described by the manufacturers.
• Transcriptional changes consistent with altered neuronal differentiation, angiogenesis, and tumor plasticity induced in human subpallial telencephalic organoid-glioblastoma chimeras

  Simone Chiola, et al., bioRxiv - Cancer Biology 2023

  Quote: ... sheep anti-TBR2 (R&D Systems, AF6166, 1:200), mouse anti-MASH1 (Invitrogen ...
• Perivascular and peribronchiolar granuloma-associated lymphoid tissue and B-cell gene expression pathways identify asymptomatic Mycobacterium tuberculosis lung infection in Diversity Outbred mice

  Deniz Koyuncu, et al., bioRxiv - Pathology 2023

  Quote: ... developed with TMB (ThermoFisher or R&D Systems), stopped using 0.25M HCl ...
• Thyroid hormone rewires cortical circuits to coordinate body-wide metabolism and exploratory drive

  Daniel R. Hochbaum, et al., bioRxiv - Neuroscience 2023

  Quote: ... Primary antibodies targeting the following proteins were used at the indicated dilutions and obtained from the denoted companies: Robo3 1:300 (R&D systems, #AF3155), GluN1 NMDAR 1:1000 (SySy #114011) ...
• False positive glycopeptide identification via in-FAIMS fragmentation

  Valentina Rangel-Angarita, et al., bioRxiv - Biochemistry 2023

  Quote: Aliquots containing 10 µg of podocalyxin (R&D Systems, 1658-PD) recombinantly expressed in NS0 cells were reconstituted at a concentration of 1 µg/µL in 50 mM AmBic and subjected to different mucinase treatments ...