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Citations for R&D Systems :
1251 - 1300 of 1581 citations for Recombinant Rat Il1b His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... BMDM were then treated with vehicle or recombinant mouse IFN-γ at 6.25 ng/ml (485-MI, R&D Systems) overnight and infected with Mtb Erdman strain at a multiplicity of infection of 5 in DMEM supplemented with 5% horse serum and 5% FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... in High Glucose DMEM supplemented with 10% FBS and 10ng/mL Recombinant Mouse CSF-1 (R&D Systems, #416-ML) for 7 days.
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bioRxiv - Developmental Biology 2023Quote: ... differentiation was induced by addition of human recombinant Activin A (ActA, 30 ng/ml, R&D systems, 338-AC-050).
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bioRxiv - Pathology 2023Quote: The ROSAKIT WT human cell line (RRID:CVCL_5G49) was cultured in the presence of recombinant human SCF (80 ng/mL, R&D Systems), as described previously (23) ...
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bioRxiv - Bioengineering 2023Quote: ... Tissue constructs were cultured in the presence of 10ng/ml TGF-β3 (human recombinant active TGF-β3, R&D Systems) for the initial 14 days ...
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bioRxiv - Molecular Biology 2023Quote: ... 10% FBS and Penicillin-Streptomycin) with or without 5 μg/ml recombinant human MANF protein (R&D systems, 3748-MN). For EdU cell proliferation assay and Ca2+ measurement ...
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bioRxiv - Physiology 2024Quote: ... 24 well-plates were coated with recombinant murine (rm) E-selectin (rmCD62E-Fc chimera, 10µg mL-1, R&D Systems) or PBS/0.1% BSA at 4°C overnight ...
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bioRxiv - Microbiology 2024Quote: ... each 25μl of pelleted resin was incubated with 2μg of recombinant human K63-linked (R&D systems, Cat# UC-320) or K48-linked (R&D Systems ...
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bioRxiv - Developmental Biology 2021Quote: ... Plasma IGF-1 concentrations were measured using the mouse/rat IGF1 Quantikine ELISA kit (R&D Systems).
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bioRxiv - Cancer Biology 2020Quote: SHP2 phosphatase activity was determined using the human/mouse/rat active DuoSet IC kit (R&D Systems). Briefly ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... α-club cell secretory protein rat monoclonal antibody (MAB4218) was from R&D Systems (Minneapolis, Minnesota, USA) and the α-Muc5B rabbit polyclonal antibody (HPA008246 ...
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bioRxiv - Cancer Biology 2022Quote: ... Specificity of staining was confirmed with the rat IgG isotype control (R&D Systems, #6-001-F). Brightfield images were acquired with a NanoZoomer-2.0 HT C9600 digital scanner (Hamamatsu ...
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bioRxiv - Neuroscience 2022Quote: ... anti-mouse IgG1 647 (Alexa Fluor R&D systems, anti-rat 555 (Alexa Fluor R&D systems) and DAPI (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... anti-mouse IgG1 647 (Alexa Fluor R&D systems, anti-rat 555 (Alexa Fluor R&D systems) and DAPI (Sigma ...
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bioRxiv - Immunology 2024Quote: ... Cryosections were stained with a monoclonal rat IgG2a anti-mouse NKp46/NCR1 (R&D Systems, clone # 29A1.4) and revealed using a donkey AF568-conjugated anti-rat pAb (Abcam) ...
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bioRxiv - Immunology 2023Quote: Serum IGF1 was measured using a Mouse/Rat DuoSet ELISA kit (R&D Systems, Minneapolis, Minnesota, USA) according to manufacturer’s instructions as previously described [14] ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... Plasma FGF21 was measured using Mouse/Rat FGF21 Quantikine ELISA Kits (R&D Systems Inc., Minneapolis, USA). Plasma leptin was measured using a Mouse Leptin ELISA Kit (Proteintech Group Inc. ...
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bioRxiv - Immunology 2021Quote: ... Nunc® Maxisorp™ 384-well plates were coated with 6 µg/ml of recombinant mouse pIgR protein (R&D Systems) diluted in coating solution concentrate (Seracare ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant human IL-21 was from Preprotech (200-21) and IL-21 neutralizing antibody was from R&D Systems (991-R2).
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bioRxiv - Cell Biology 2022Quote: ... The collagen-cell mixture was then casted in custom-made chamber and collagen was allowed to polymerize for 45min at 37°C before overlaying the gel with either 0.6ug/ml of recombinant mouse CCL19 (for DCs, 440-M3-025, R&D Systems), 25nM fMLP (for neutrophil-like cells ...
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bioRxiv - Cell Biology 2019Quote: ... C2C12 and cardiac fibroblasts were serum starved for 24 h before adding medium containing 5 ng/ml recombinant TGFβ1 for 2 days (R&D Systems, Minneapolis ...
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bioRxiv - Biochemistry 2020Quote: ... and the lysate (100 μg protein) or 4 μg of recombinant human BACE1 (Cat. # 931-AS; R&D Systems, Minneapolis, MN) in extraction buffer was incubated at 37°C for 1 h with a secretase-specific peptide conjugated to two reporter molecules ...
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bioRxiv - Neuroscience 2021Quote: ... Sigma-Aldrich)-coated dishes in DMEM/F12/ B27/N2-medium supplemented with 200 ng/ml human recombinant sonic hedgehog (SHH, R&D Systems) and 100 ng/ml murine recombinant fibroblast growth factor 8b (FGF-8b ...
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bioRxiv - Immunology 2021Quote: ... In another experiment mice were treated intravenously 2 days post infection with 12.5 μg/kg recombinant murine IL-23 (1887-ML-010/CF, R&D Systems) for 3 consecutive days.
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bioRxiv - Cell Biology 2020Quote: ... media was supplemented with 5 ng/mL recombinant human TGFβ-1 (R&D Systems; resuspended in 4 mM HCl, 0.1% BSA).
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bioRxiv - Microbiology 2020Quote: ... mouse anti-ATG12 (Cat# MAB6807) and recombinant mouse IFN-α (Cat #:12100-1) from R&D Systems (Minneapolis, MN 55413, USA). The mouse anti-CHIKV (Clone A54Q ...
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bioRxiv - Immunology 2022Quote: Cell surface staining for the anti-HER2 BCR was performed with Recombinant Human ErbB2/Her2 Fc Chimera Protein (R&D Systems) and APC anti-His Tag antibody (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... cells were divided and incubated in the presence or absence of 125 ng/ml recombinant TNF (R&D Systems, Minneapolis, MN) for 24 additional hours ...
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PVT1, a YAP1 dependent stress responsive lncRNA drives ovarian cancer metastasis and chemoresistancebioRxiv - Cancer Biology 2022Quote: ... epithelial to mesenchymal transition (EMT) was induced with 200 pM of Human recombinant TGF-beta 1 (R&D Systems, 240-B); verteporfin (Sigma Aldrich ...
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bioRxiv - Neuroscience 2019Quote: WT and T1KO mice received injections (s.c.) of recombinant murine (rm)TIMP-1 (10 ng/μL, 10 μL; R&D Systems; Minneapolis ...
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bioRxiv - Immunology 2020Quote: ... C2C12 myotubes were treated with this concentration (7.7 nM) of recombinant mono- or bivalent antibodies in the absence or presence of 0.1 nM neural agrin (R&D systems). MuSK was immunoprecipitated from whole lysate and detected on western blot ...
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bioRxiv - Immunology 2020Quote: ... Cells were stained with 25 μL of 0.5 μg/mL primary anti mouse-TLR4 (recombinant monoclonal rabbit IgG, clone #1203B, R&D Systems) or isotype (normal polyclonal rabbit IgG control ...
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bioRxiv - Cell Biology 2019Quote: ... Assay protocol is based on Nakamura et al (63) with buffer modifications provided by the recombinant Wnt manufacturer (R&D Systems). A more detailed protocol is available upon request ...
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bioRxiv - Cell Biology 2021Quote: ... media was supplemented with 5 ng/mL recombinant human TGFβ-1 (R&D Systems; resuspended in 4 mM HCl, 0.1% BSA). MCF7 ...
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bioRxiv - Immunology 2020Quote: ... ELISA was performed in 96-well plates coated with 1 μg/mL of human recombinant IL13Rα2hFc (R&D Systems, Minneapolis, MN). Following blocking with PBS/2%FBS and washes with TBS-Tween 20 buffer (Boston Bioproducts ...
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bioRxiv - Molecular Biology 2021Quote: Recombinant human transforming growth factor-β (TGF-β) and fibroblast growth factor-2 FGF-2 were purchased from R&D Systems. BTZ ...
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bioRxiv - Immunology 2021Quote: ... recombinant proteins representing either full-length mouse ANGPTL3 or the N-terminal domain of mouse ANGPTL3 (R&D Systems, Minneapolis, MN), or the N-terminal domain of human ANGPTL4 (R&D Systems ...
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bioRxiv - Neuroscience 2023Quote: ... Purified recombinant NRX1β ectodomain fused to human immunogloblin Fc (NRX1β-Fc, R&D sytems, Cat#5268-NX-05, R&D system) was immobilized on carboxymethylated dextran CM5 sensor chips (Cytiva ...
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bioRxiv - Neuroscience 2022Quote: ... the media was incubated (72 h) with media containing different concentration of recombinant human PTN (rhPTN, R&D systems 252 - PL) or the drugs alectinib (Toronto Research Chemicals ...
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bioRxiv - Molecular Biology 2022Quote: ... 96 wells-plate were used to plate the cells at 10,000 cells/well in RPMI +10% FBS supplemented or not by different concentrations of recombinant Tpo (R&D Systems). After 72 hours ...
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bioRxiv - Developmental Biology 2024Quote: ... Serial dilutions from 2μg to 0.0625μg of recombinant SPARC (SRP3159, Sigma-Aldrich; S5174-25UG, Sigma-Aldrich; 942-SP-050, R&D Systems) were spotted and used as standards ...
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bioRxiv - Microbiology 2024Quote: ... Monocytes were differentiated into hMDMs for at least 7 days in complete medium containing 10 ng.mL-1 of recombinant human macrophage colony-stimulating factor (rhM-CSF; R&D systems).
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bioRxiv - Developmental Biology 2023Quote: ... NSPC culture in adherent and non-adherent proliferating conditions using media supplemented with human recombinant EGF and FGF2 (R&D Systems) was performed according to published protocols (Soldati et al ...
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CXCL17 binds efficaciously to glycosaminoglycans with the potential to modulate chemokine signallingbioRxiv - Immunology 2023Quote: ... aligned and then allowed to migrate for 60 minutes along chemokine gradients generated by the addition of 1µl of recombinant 10 nM CXCL8 or varying concentrations of CXCL17 (24-119) purchased from R&D Systems. Basal migration was recorded in the absence of stimulus ...
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bioRxiv - Bioengineering 2023Quote: ... Fibroblasts were treated with or without recombinant human transforming growth factor beta 1 (TGF-β1) protein (240-B, R&D Systems) at 1.25 ng/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... into 40 ml of R10 media supplemented with 10 ng/ml recombinant human IL-2 (R&D Systems, 202-IL-050). Cells were passaged twice per week and feeder cells were added in a 1:1 ratio after 7 days to maintain stimulation ...
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bioRxiv - Cell Biology 2022Quote: ... Experiments were done in 293T cells pretreated with 5 ng/ml recombinant human R-spondin 3 (R&D Systems, Minneapolis, USA). Experiments were performed with biological triplicates ...
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bioRxiv - Cell Biology 2023Quote: ... 10 ng/mL heregulin-1β (recombinant human NRG-beta 1/HRG-beta 1 EGF domain; R&D Systems, Minneapolis, MN, USA), 200 ng/mL recombinant human IGF-1 (LONG R3-IGF-1 ...
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bioRxiv - Immunology 2023Quote: ... Cells were cultured for 4 days in the presence of 20ng/ml murine recombinant IL-12 (R&D systems; 419-ML). The proportion of IFNγ-secreting CD4+ T-cells (Th1 cells ...
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bioRxiv - Physiology 2023Quote: ... Activity was calculated based on a standard curve determined using purified recombinant mouse myeloperoxidase protein (R&D Systems; Catalog# 3667-MP).