Mouse anti Rift Valley Fever Virus Nucleoprotein M976 citations for R&D Systems

Structure and function of the ROR2 cysteine-rich domain in vertebrate noncanonical WNT5A signaling

Samuel C. Griffiths, et al., bioRxiv - Biochemistry 2021

Quote: The following recombinant proteins and drugs were purchased: human/mouse WNT5A (654-WN-010, R&D Systems); Wnt-C59 (C7641-2s ...

Urokinase receptor associates with TLR4 interactome to promote LPS response

Yulia Kiyan, et al., bioRxiv - Cell Biology 2020

Quote: Unconjugated and Alexa 647-conjugated mouse uPAR antibody were from R&D systems (MAB531 and FAB531R, respectively); TLR4 antibody (MAB27591 ...

Macrophage Inflammatory State Influences Susceptibility to Lysosomal Damage

Amanda O. Wong, et al., bioRxiv - Immunology 2020

Quote: ... and mouse IL-10 cytokine concentrations were determined using ELISA DuoSet kits (R&D Systems, Minneapolis, MN).

An anti-ACVR1 antibody exacerbates heterotopic ossification by fibro/adipogenic progenitors in fibrodysplasia ossificans progressiva mice

John B. Lees-Shepard, et al., bioRxiv - Developmental Biology 2021

Quote: ... clones were treated with a dilution series of recombinant mouse BMP9 (R&D Systems #5566-BP-010) ranging from100 ng/ml to 0.1 ng/ml (4.1 nM to 4.1 pM ...

PRMT5 in T cells drives Th17 responses, mixed granulocytic inflammation and severe allergic airway inflammation

Brandon W. Lewis, et al., bioRxiv - Immunology 2021

Quote: ... CXCL1 was measured in BAL using the mouse CXCL1 Quantikine ELISA kit (R&D Systems, Minneapolis, MN) following manufacturer’s instructions.

A stimulus-contingent positive feedback loop enables IFN-β dose-dependent activation of pro-inflammatory genes

Catera L. Wilder, et al., bioRxiv - Immunology 2022

Quote: ... and 100 ng/ml recombinant mouse IL-28B/IFN-λ3 (R&D Systems #1789-ML-025/CF). For select experiments ...

Intestinal epithelial MHC class II regulation by HDAC3 instructs microbiota-specific CD4+ T cells

Emily M. Eshleman, et al., bioRxiv - Immunology 2022

Quote: ... Supernatants were collected and lipocalin-2 levels were determined using mouse Lipocalin-2/NGAL (R&D Systems) following the manufacturer’s instructions.

Combinatorial microRNA loading into extracellular vesicles for anti-inflammatory therapy

Alex Eli Pottash, et al., bioRxiv - Bioengineering 2022

Quote: ... IL-6 concentration was determined using the Mouse IL-6 DuoSet ELISA Kit (R&D Systems; DY406). Cell transfection was achieved using HiPerfect (Qiagen ...

Regulation of anterior neurectoderm specification and differentiation by BMP signaling in ascidians

Agnès Roure, Rafath Chowdhury, Sébastien Darras, bioRxiv - Developmental Biology 2022

Quote: ... Embryos were treated with 150 ng/ml of recombinant mouse BMP2 protein (355-BEC, R&D Systems Inc ...

Regulation of neuropathic pain by microglial Orai1 channels

Shogo Tsujikawa, et al., bioRxiv - Neuroscience 2022

Quote: ... Supernatants were subsequently analyzed using mouse ELISA kits for the release of TNFα (R&D Systems, MTA00B), IL-6 (RayBiotech ...

Defining cell type-specific immune responses in a mouse model of allergic contact dermatitis by single-cell transcriptomics

Youxi Liu, et al., bioRxiv - Immunology 2024

Quote: ... dFBs were treated with either recombinant mouse IFNγ (5 ng/ml, R&D Systems, Minneapolis, MN, USA) for 8 or 24 hours before being subjected to qRT-PCR or ELISA analysis.

Regulation of fatty acid delivery to metastases by tumor endothelium

Deanna N. Edwards, et al., bioRxiv - Cancer Biology 2024

Quote: ... Cells were stimulated with recombinant mouse VEGF-A (300 ng/mL, R&D Systems #493-MV-005), recombinant mouse VEGF-B186 (300 ng/mL) ...

Astrocytic Slc4a4 regulates blood-brain barrier integrity in healthy and stroke brains via a NO-CCL2-CCR2 pathway

Qi Ye, et al., bioRxiv - Neuroscience 2023

Quote: ... Conditioned media was then measured in duplicates with Mouse Cytokine Array Panel A (ARY006, R&D Systems) according to the manufacturer’s instructions ...

Mice with renal-specific alterations of stem cell-associated signaling develop symptoms of chronic kidney disease but surprisingly no tumors

Adam Myszczyszyn, et al., bioRxiv - Cell Biology 2023

Quote: ... EPO concentrations in supernatants (plasma) were measured using the Quantikine Mouse Erythro-poietin Immunoassay (R&D Systems) according to the manufacturer’s instructions.

Cross-species comparison of pregnancy-induced GDF15

Anders Bue Klein, et al., bioRxiv - Physiology 2023

Quote: ... plasma samples were analyzed using Quantikine ELISA Mouse GDF15 Immunoassay (ELISA, R&D systems, catalog no. MGD150). The ELISA assays were used according to the protocol provided by the manufacturer.

LRRK2 regulates the activation of the unfolded protein response and antigen presentation in macrophages during inflammation

Ahmed M. Fahmy, et al., bioRxiv - Immunology 2023

Quote: Collected supernatants were assayed for IL-6 and IFN-β using mouse DuoSet kits (R&D Systems). Samples were diluted in PBS and incubated in a 96-well plate pre-coated with either IL-6 or IFN-β capture antibodies for 2h at room temperature ...

Regulation of Lung Immune Tone by the Gut-Lung Axis via Dietary Fiber, Gut Microbiota, and Short-Chain Fatty Acids

Daisuke Maruyama, et al., bioRxiv - Immunology 2023

Quote: ... plasma and lung homogenate were determined using the mouse DuoSet ELISA kit (R&D Systems, Minneapolis, MN). All assays were performed according to the manufacturer’s supplied protocol ...

Oct1 cooperates with Smad transcription factors to promote mesodermal lineage specification

Jelena Perovanovic, et al., bioRxiv - Developmental Biology 2023

Quote: ... transfected cells were provided with 5 ng recombinant mouse TGFβ1 protein (R&D Systems, 7666-MB-005). mCherry fluorescence was determined first by exciting at 570 nm and measuring emission at 610 nm with a 100 msec time delay using a Perkin-Elmer EnVision Xcite Multilabel Plate Reader ...

The SOCS1 KIR and SH2 domain are both required for suppression of cytokine signaling in vivo

Karen Doggett, et al., bioRxiv - Cell Biology 2022

Quote: ... BMDMs were treated with 100 ng/mL mouse IFNγ or 5 U/mL IFNα (R&D Systems) for 2 ...

Antimicrobial overproduction sustains intestinal inflammation by inhibiting Enterococcus colonization

Kyung Ku Jang, et al., bioRxiv - Immunology 2023

Quote: ... LCN2 in the clear supernatants was quantified using Mouse Lipocalin-2/NGAL DuoSet ELISA (R&D systems) according to the manufacturer’s instructions.

Intraperitoneal transfer of wild-type bone marrow cells in the Csf1r knockout rat repopulates resident tissue macrophages without contributing to monocytopoiesis

Anuj Sehgal, et al., bioRxiv - Immunology 2023

Quote: Serum IGF1 was measured using a Mouse/Rat DuoSet ELISA kit (R&D Systems, Minneapolis, Minnesota, USA) according to manufacturer’s instructions as previously described [14] ...

Reactive astrocytes associated with prion disease impair the blood brain barrier

Rajesh Kushwaha, et al., bioRxiv - Neuroscience 2023

Quote: ... ELISA kit for IL-6 and recombinant mouse IL-6 wrtr from R&D Systems (Minneapolis, MN). Bicinchoninicacid (BCA ...

Three-Dimensional Bioconjugated Liquid-Like Solid (LLS) Enhance Characterization of Solid Tumor - Chimeric Antigen Receptor T cell interactions

Duy T. Nguyen, et al., bioRxiv - Bioengineering 2023

Quote: ... including inflammatory cytokines and chemokines using mouse IFNg DuoSet ELISA kit (R&D Systems, Minneapolis, MN, USA). In addition ...

Naïve Huntington’s disease microglia mount a normal response to inflammatory stimuli but display impaired development of innate immune tolerance that can be counteracted by ganglioside GM1

Noam Steinberg, et al., bioRxiv - Neuroscience 2023

Quote: ... Recombinant mouse granulocyte-macrophage colony-stimulating factor (GM-CSF) was purchased from R&D systems (415ML/CF), recombinant human Interferon gamma1b (IFNγ1b ...

Coxiella burnetii actively blocks IL-17-induced oxidative stress in macrophages

Tatiana M. Clemente, et al., bioRxiv - Microbiology 2023

Quote: ... cells were stimulated or not with 100 ng/ml mouse IL-17 recombinant protein (R&D Systems) for 10 or 30 min ...

Reactive nitrogen species inhibit branched chain alpha-ketoacid dehydrogenase complex and impact muscle cell metabolism

Nicholas L. Arp, et al., bioRxiv - Biochemistry 2023

Quote: ... 20 ng/mL recombinant mouse tumor necrosis factor-α (TNF-α) (R&D Systems, 41-0MT0-25CF) and 12 ng/mL recombinant mouse IFN-γ were added to the media.

The anti-obesity effect of fish oil in diet-induced obese mice occurs via the induction of heat production in brown but not white adipose tissue

Takahiko Obo, et al., bioRxiv - Animal Behavior and Cognition 2023

Quote: ... Plasma FGF21 was measured using Mouse/Rat FGF21 Quantikine ELISA Kits (R&D Systems Inc., Minneapolis, USA). Plasma leptin was measured using a Mouse Leptin ELISA Kit (Proteintech Group Inc. ...

Local Delivery of SBRT and IL12 by mRNA Technology Overcomes Immunosuppressive Barriers to Eliminate Pancreatic Cancer

Angela L. Hughson, et al., bioRxiv - Cancer Biology 2023

Quote: ... Magnetic Luminex Assays were preformed using a Mouse Premixed Cytokine/Chemokine Multi-Analyte kit (R&D Systems) according to manufacturer’s instructions ...

ERK1/2 Inhibition Alleviates Noise-Induced Hearing Loss While Tempering Down the Immune Response

Richard D. Lutze, et al., bioRxiv - Neuroscience 2023

Quote: ... Tissues were stained overnight at 4°C with mouse CD45 antibody (1:200; Af114, R&D Systems). The next day ...

PD-L1 Restrains the PD-1^hiNrp1^loTGFβ⁺ Treg to block IL6⁺ Neutrophil tumor infiltration to Suppress Inflammation-driven Colorectal Cancer

Dakota B. Poschel, et al., bioRxiv - Immunology 2023

Quote: ... Treg were differentiated from the purified CD4+ cells using CellXVivo Mouse Treg differentiation kit (R&D System). Briefly ...

Inhibition of the Eukaryotic Initiation Factor-2-α Kinase PERK Decreases Risk of Autoimmune Diabetes in Mice

Charanya Muralidharan, et al., bioRxiv - Cell Biology 2024

Quote: ... the proinflammatory cytokine cocktail contained 25 ng/mL mouse IL-1β (R&D Systems; 401-ML-010), 50 ng/mL mouse TNF-α (R&D Systems ...

Oseltamivir (Tamiflu), a Commonly Prescribed Antiviral Drug, Mitigates Hearing Loss in Mice

Emma Sailor-Longsworth, et al., bioRxiv - Neuroscience 2024

Quote: ... Tissues were stained overnight at 4°C with mouse CD45 antibody (1:50; Af114, R&D Systems). The next day ...

Sex-specific resilience of neocortex to food restriction

Zahid Padamsey, et al., bioRxiv - Neuroscience 2024

Quote: Serum leptin levels were analyzed using ELISA kits (Mouse Leptin Quantikine Elisa Kit, R&D Systems, USA) according to manufacturer’s instruction ...

Epstein-Barr virus induced 3 attributes to TLR7-mediated splenomegaly and bicytopenia

Masanori Iseki, et al., bioRxiv - Immunology 2024

Quote: ... and then stimulated with recombinant mouse IL-27 (100 ng/ml, R&D Systems, Minneapolis, MN, USA) for 6 hours ...

Meiotic DSB-independent role of protein phosphatase 4 in Hop1 assembly to promote meiotic chromosome axis formation in budding yeast

Ke Li, Miki Shinohara, bioRxiv - Molecular Biology 2022

Quote: ... The primary antibodies used in these assays were anti-γH2A (anti-γH2A, AF2288, R&D Systems, 1:1000), anti-Myc (MC045 ...

Klebsiella pneumoniae hijacks the Toll-IL-1R protein SARM1 in a type I IFN-dependent manner to antagonize host immunity

Claudia Feriotti, et al., bioRxiv - Microbiology 2021

Quote: The following antibodies were used: anti-IL-1β (anti-goat, 1:1000; # AF-401-NA, R&D Systems), anti-caspase-1 (anti-rabbit ...

Effects of decreased Rac activity and malignant state on oral squamous cell carcinoma

Hani Al-Shareef, et al., bioRxiv - Cancer Biology 2019

Quote: ... Both the anti-JNK and anti-phospho-JNK (Thr183/Tyr185) polyclonal antibodies were purchased from R&D Systems, Inc ...

Polycomb repressive complex 1.1 coordinates homeostatic and emergency myelopoiesis

Yaeko Nakajima-Takagi, et al., bioRxiv - Immunology 2022

Quote: ... then stained with biotinylated anti-lineage antibody cocktail and anti-c-Kit antibody (#AF 1356; R&D Systems), or anti-FcγR-AlexaFluor 647 (#101314 ...

MerTK mediates the immunologically silent uptake of alpha-synuclein fibrils by human microglia

Marie-France Dorion, et al., bioRxiv - Cell Biology 2023

Quote: ... and stained with the following antibodies: anti-AXL clone #108724 and anti- MERTK clone #125518 (R&D Systems). Appropriate forward and side scatter profiles were used to exclude debris and doublets from the analysis ...

ICELLNET: a transcriptome-based framework to dissect intercellular communication

Floriane Noël, et al., bioRxiv - Systems Biology 2020

Quote: ... an APC-anti-ICOSL (R&D Systems) and Alexa-Fluor-700-anti-HLA-DR (Biolegend ...

Microplastic consumption induces inflammatory signatures in the colon and prolongs a viral arthritis

Daniel J. Rawle, et al., bioRxiv - Immunology 2021

Quote: ... goat anti-NKp46 (R&D Systems, AF2225), or rat anti-Ly6G (ab2557 NMP-R14 ...

Heterozygous loss of ZBTB38 leads to early embryonic lethality in mice via suppressing Nanog and Sox2

Miki Nishio, et al., bioRxiv - Developmental Biology 2021

Quote: ... anti-Oct3/4 (MAB1759, R&D Systems), anti-Sox2 (S1451 ...

A TgfβRI/Snai1-dependent developmental module at the core of vertebrate axial elongation

André Dias, et al., bioRxiv - Developmental Biology 2020

Quote: ... goat anti-Tbx6 (R&D Systems, AF4744), rabbit anti-Laminin 111 which detects all laminins containing a1 ...