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Citations for R&D Systems :
1151 - 1200 of 1419 citations for 5H Cyclopenta b pyridine 3 carboxylicacid 2 amino 6 7 dihydro ethylester 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2019Quote: ... Cell lines were validated for pluripotency markers by immunhohistochemical stainings using the Human Pluripotent Stem Cell 3-Color Immunohistochemistry Kit (R&D Systems, SC021) and were differentiated into the three different germ layers using the Human Pluripotent Stem Cell Functional Identification kit (R&D Systems ...
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bioRxiv - Cell Biology 2021Quote: ... HTM cells were cultured in serum-free DMEM with 1% PSG and subjected to the different treatments for 3 d: TGFβ2 (2.5 ng/ml; R&D Systems, Minneapolis, MN, USA), the ERK inhibitor U0126 (10 μM ...
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bioRxiv - Neuroscience 2022Quote: Day 14 MGL media samples collected after 3 and 24h of IL-6 exposure and pooled from one donor over 3 harvests were incubated with Proteome Profiler Human Cytokine antibody array membranes (R&D Systems; ARY005B), as per the manufacturers instructions ...
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bioRxiv - Cell Biology 2023Quote: ... 3 independent vials of iCell microglia were seeded at 5 x 104 cells per well across 3 independent 96-well plates and treated the following day with vehicle or 5 ng/mL human IFN-β (R&D Systems). Cells were treated for 6 or 24 h prior to collection and stored at −80°C prior to RNA extraction.
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bioRxiv - Bioengineering 2023Quote: ... The relative expression levels of apoptosis related proteins of post I/RI iCMs (n=3) were analyzed using the Proteome Profiler Human Apoptosis Array (R&D Systems, #ARY009), according to the manufacturer’s instructions.
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bioRxiv - Immunology 2019Quote: ... sections were incubated overnight at 4°C with an antibody mix containing rat anti-mouse IL-18 (5 μg/ml; D047-3; R&D Systems, MN, USA) and rabbit anti-EpCAM (Clone ab71916 ...
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bioRxiv - Cell Biology 2019Quote: ... and expanded chondroprogenitor cells were re-suspended at 2.5 × 105 cells / mL in chondrogenic medium supplemented with 10 ng/ml human transforming growth factor beta 3 (TGF-β3; R&D Systems, Minneapolis, MN). Chondrogenic pellets were cultured at 37°C for 28 days ...
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bioRxiv - Biophysics 2020Quote: ... cells were changed to long term DMEM/F12 media50 in the presence or absence of 3 ng/ml TGF-β1 (R&D Systems, Abingdon, UK). Media was replenished three times per week ...
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bioRxiv - Microbiology 2021Quote: ... S1-Fc (Catalog # 10623-CV, lot DONK0120091) and S1-biotin (Catalog # BT10569, lot DOJW120092) were purchased from R&D Systems (Vendor #3; Minneapolis, MN). Streptavidin (Catalog# 85878-10MG ...
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bioRxiv - Pathology 2022Quote: ... BMMs were plated into 96-well or 24-well plates (3 × 104 cells per milliliter) in complete α-MEM culture media supplemented with 50 ng/mL RANKL (R&D Systems, USA) and 25 ng/mL M-CSF (PeproTech ...
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bioRxiv - Bioengineering 2019Quote: hESCs were differentiated to SC-β cells as previously described9 by culturing in the following conditions in order: Stage 1: 3 d with 100 ng/ml Activin A (R&D Systems; 338-AC) and 3 μM CHIR99021 (Stemgent ...
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bioRxiv - Neuroscience 2023Quote: ... On days 27 to 40 organoids were fed on alternate days with media containing 20 ng/mL NT-3 (R&D Systems, 267-N3) and 10 ng/mL BDNF (R&D Systems 248-BD) ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.3% Triton X-100) for 1 hour at room temperature and incubated with primary antibody against VE-cadherin (AF938, R&D Systems, 1:100) overnight at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were treated with 3 uM CHIR, 300 nM LDN193189 (LDN, Axon, Scottsdale, AZ) and 100 ng/mL epidermal growth factor (EGF, R&D systems, Minneapolis, MN) in Advanced DMEM/F12 (Invitrogen ...
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bioRxiv - Bioengineering 2024Quote: ... Stem cells underwent directed differentiation into definitive endoderm over a 3-day treatment using Activin A (100ng/mL, R&D Systems Cat#338-AC) added to RPMI base media ...
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bioRxiv - Cell Biology 2022Quote: ... washed with PBS and incubated with recombinant Jagged 1 protein (JAG1, 2 µg/ml, R&D System) for 3 h at room temperature ...
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bioRxiv - Immunology 2022Quote: ... CFSE-labelled cells were then resuspended in R10 media supplemented with recombinant IL-2 (R&D system) and 2-mercaptoethanol (Themo Fisher Scientific ...
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bioRxiv - Physiology 2019Quote: ... for 72 hours prior to stimulation with 10 ng/mL IFN-α (R&D Systems, 11101-2). Media was prepared by adding 120 μL of 10 mg/mL stock 13C11 15N2 L-tryptophan in 1x PBS to 240 mL of RPMI (Corning ...
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bioRxiv - Immunology 2021Quote: ... and 2.5 ng/ml recombinant mouse IL-15 and 100 units of IL-2 (R&D Systems).
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bioRxiv - Bioengineering 2021Quote: ... ELISA kits for FGF-2 and TGF-β1 were purchased from R&D Systems (Minneapolis, MN, USA) while the ELISA kit for Nodal was obtained from Abcam (Cambridge ...
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bioRxiv - Cell Biology 2020Quote: Supernatant levels of BMP2 were quantified using a BMP-2 Quantikine ELISA kit (R&D Systems #DBP200), without significant deviations from manufacturer’s instructions.
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bioRxiv - Physiology 2020Quote: ... Each treatment infusion was 2 μL of PBS or leptin (10μg/2μL, R&D Systems, Minneapolis, MN). Data collected 30 minutes prior and 30 minutes after the ICV injections were excluded from the analysis ...
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bioRxiv - Microbiology 2020Quote: ... The transduced cells were stained with anti-human ACE-2 polyclonal goat IgG (AF933, R&D Systems) primary antibody at 1 ug/mL and donkey anti-goat IgG conjugated to Alexa Fluor 488 (ab150129 ...
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bioRxiv - Immunology 2022Quote: ... The binding kinetics for the interaction between hmAbs and SARS-CoV-2 Spike protein (R&D Systems) was determined by injecting four concentrations of SARS-CoV-2 Spike (25 nM highest concentration ...
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bioRxiv - Cell Biology 2022Quote: ... mice were given intra-articular injections of recombinant mouse R-spondin 2 (500 ng, R&D Systems) or vehicle (phosphate-buffered saline ...
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bioRxiv - Cancer Biology 2021Quote: ... a standard direct ELISA was performed with DuoSet Ancillary Reagent Kit 2 (R&D systems, Minneapolis, MN). After coating wells with recombinant human EGFR (1μg/mL) ...
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bioRxiv - Cell Biology 2019Quote: ... the substrates were incubated for 2 h with human E-cadherin-Fc (R&D system, Minneapolis, MN) at 1 μg / cm2 ...
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bioRxiv - Developmental Biology 2021Quote: ... Media was changed every 2 days using differentiation media supplemented with 100ng/ml VEGF (R&D Systems) and 50ng/ml bFGF (R&D Systems) ...
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bioRxiv - Immunology 2022Quote: ... 1% PS and 100 IU/ml of human recombinant IL-2 (R&D Systems, 202-IL-050). Cells were used at low passage numbers ...
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bioRxiv - Microbiology 2022Quote: ... n = 4 each) were stained with mouse anti-SARS-CoV-2 N monoclonal antibody (R&D systems, Clone 1035111 ...
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bioRxiv - Immunology 2022Quote: ... Recombinant Human IL-27 Ra/WSX-1/TCCR Fc Chimera Protein (2 μg/ml, R&D Systems), Ultrapure LPS from E ...
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bioRxiv - Immunology 2023Quote: ... LCN2 in the clear supernatants was quantified using Mouse Lipocalin-2/NGAL DuoSet ELISA (R&D systems) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ELISA plates were coated with 2 µg/ml mouse anti-His tag antibody (R&D Systems, MAB050) and blocked as described above ...
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bioRxiv - Biochemistry 2023Quote: The human ADGF (Recombinant Human Adenosine deaminase 2/CECR1) #7518-AD-010 was from R&D Systems, Minneapolis ...
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bioRxiv - Microbiology 2020Quote: ... Primary BMDCs were isolated from C57BL/6 mice and cultured in Dulbecco’s modified Eagle medium for 3–5 days in the presence of 20 ng/mL recombinant granulocyte-macrophage colony-stimulating factor (R&D Systems, Minneapolis, MN, USA) as described previously (83) ...
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bioRxiv - Cell Biology 2019Quote: ... Antibodies used for ChIP (3 μg per reaction) were mouse monoclonal IgG2a including: 1) anti-NR1H3 (clone PPZ0412, R&D Systems catalog PP-PPZ0412-00), 2 ...
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bioRxiv - Microbiology 2020Quote: ... were cultured in 24-well tissue dishes for 3 days in RPMI1640 medium supplemented with 100ng/ml Activin A (R&D Systems, 338-AC-050) and 2µM CHIR99021 (Tocris ...
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bioRxiv - Microbiology 2021Quote: ... bone marrow cells were cultured in RPMI-1640 medium supplemented with 10% FBS in the presence of FMS-like tyrosine kinase 3 ligand (Flt3L) (100 ng/ml, R&D Systems, Cat# 308-FK) for 7-9 days ...
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bioRxiv - Immunology 2022Quote: ... To assess the cytokine profile of the different conditions plasma and PBMC conditioned media from 3 donors were pooled separately and subjected to the Proteome Profiler Assay Human XL Cytokine Array Kit (R&D Systems, Minneapolis, MN, USA). The assay was performed according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... tumors were sectioned at 3 µm and stained using the Bond-Max device (Leica) (CD4: Cell Signaling Technology, 25229S; CD8a: Synaptic Systems, 361003; FoxP3: R&D Systems, MAB8214 ...
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bioRxiv - Cancer Biology 2023Quote: Ascites collected from RD and IF mice (n=4/ group) were pooled and diluted 1:3 and applied in the adipokine array (ARY-013, R&D systems, Minneapolis, MN, USA). The proteome profiler adipokine array detects 38 adipokines (Table S4 ...
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bioRxiv - Cancer Biology 2024Quote: ... To this goal we used a specific spectrofluorimetric activity assay (Human Active Caspase-3 Immunoassay Quantikine ELISA, Catalog Number KM300, R&D Systems, Inc., Minneapolis, MN). In summary ...
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bioRxiv - Immunology 2024Quote: ... 1:100 diluted human plasmas and a dilution series of the indicated neutralizing anti-IFN antibodies (IFNβ, PBL assay science, 31410-1; IFNλ1: R&D systems, MAB15981; IFNλ2/3: R&D systems, MAB1587), or mock ...
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bioRxiv - Cancer Biology 2024Quote: Xenograft studies were performed by subcutaneous bilateral implantation of 5E6 tumor cells/injection suspended 100 µl of 1: 1 RPMI / BME type 3 (R&D Systems #36-320-1002P). 4 to 6-week-old NOD-scid IL2Rgnull (NSG ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were embedded in Cultrex® RGF BME Type 2 (cat no. 3533-005-02, R&D systems) and placed in a 37°C incubator for 20 min ...
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bioRxiv - Genomics 2019Quote: ... the medium was changed to STEMdif APEL 2 with 200 ng/mL FGF9 (R&D Systems, Minneapolis, MN) and 1 mg/mL heparin (StemCell Technologies ...
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bioRxiv - Immunology 2019Quote: Myeloperoxidase (MPO) and Lipocalin-2 were measured using ELISAs and following the manufacturers’ recommendations (from R&D Systems).
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bioRxiv - Microbiology 2021Quote: ... and FABP-2 were measured from plasma using standard ELISA kits from R&D Systems (DC140, DC1630 &DFBP20). Positive testing controls for each ELISA kit were also included (R&D Systems QC20 ...
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Direct intracellular visualization of Ebola virus-receptor interaction by in situ proximity ligationbioRxiv - Microbiology 2020Quote: ... or recombinant human cathepsin L (CatL, 2 ng/ul, pH 5.5, 37°C for 1h; R&D Systems) as described previously (5) ...
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bioRxiv - Cancer Biology 2020Quote: ... mouse tumors were homogenized with a tissue homogenizer in 700μL of Cell Lysis Buffer 2 (R&D Systems) containing 1x Halt Protease Inhibitor Cocktail (ThermoFisher Scientific) ...