Labshake search
Citations for R&D Systems :
8751 - 8800 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... one hundred microliter of pre-warmed complete medium containing 10 ng/mL recombinant mouse IL-2 (rmIL-2; 402-ML-020/CF, R&D Systems) or 20 ng/mL rmIL-7 was added to each Nucleocuvette™ strip well for activated or naïve CD8+ T cells ...
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bioRxiv - Immunology 2021Quote: ... magnetically isolated T cells were resuspended in the complete medium and were seeded in a 10-cm petri dish or T-75 flask at 1–2×106 cells/cm2 and cultured in the presence of 20 ng/mL recombinant mouse interleukin-7 (rmIL-7; 402-ML-020/CF, R&D Systems, Minneapolis, MN) for 24 hr at 37°C in a humidified 5% CO2 atmosphere.
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bioRxiv - Immunology 2021Quote: ... and ICAM (50μM; R&D Systems) coated 8-chamber live imaging culturing slides (μ-Slide 8 Well ...
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bioRxiv - Immunology 2021Quote: ... with and without CD80 (R&D Systems rhCD80-Fc ...
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bioRxiv - Immunology 2021Quote: ... supplemented with 20% heat-inactivated FBS (R&D Systems), 1% penicillin-streptomycin (Gibco) ...
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bioRxiv - Immunology 2021Quote: ... 20% Heat-inactivated FBS (R&D Systems) and 1% pen/strep (ThermoFisher scientific ...
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bioRxiv - Immunology 2021Quote: ... for M1-polarization or with 50 ng/ml IL-6 (R&D Systems, Minneapolis, MN, USA) and 20 ng/ml IL-4 (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: ... and ELISA (human/mouse/rat activin A Quantikine ELISA kit, R&D Systems, #DAC00B) was performed to measure Activin A production according to the manufacturer’s protocol.
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bioRxiv - Immunology 2021Quote: ... and then treated with or without 10ng/ml IL-1α (R&D Systems, #200-LA-CF) or TNFα (R&D Systems ...
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bioRxiv - Immunology 2021Quote: ... or TNFα (R&D Systems, #210-TA-CF) for five days ...
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bioRxiv - Immunology 2021Quote: ... and 500 ng/ml soluble anti-CD28 and cultured in the absence (Th0) or presence of 2.5 ng/ml IL-12 (R&D Systems) (Th1 ...
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bioRxiv - Immunology 2021Quote: ... IL-1β (10ng/ml, R&D Systems Cat # 201 LB); TGF-β1 (10ng/ml ...
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bioRxiv - Immunology 2021Quote: ... TGF-β1 (10ng/ml, R&D Systems Cat# 240); anti-IL-4 (1 μg/ml ...
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bioRxiv - Immunology 2021Quote: ... or 10 ng/ml IL-4 (R&D Systems) (for Th2) ...
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bioRxiv - Immunology 2021Quote: ... supernatants were harvested to evaluate hIL-8 secretion by enzyme-linked immunosorbent assay (ELISA) according to manufacturer’s instructions (R&D Systems, U.S.A.). Cell protein concentrations were determined using the BCA Protein Assay kit (Thermofisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... or isotype goat polyclonal isotype control (R&D Systems, Cat # AB-108-C) at 1:100 dilution for 30 minutes on ice ...
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bioRxiv - Immunology 2021Quote: ... cells were washed twice in PBS and stained with a goat anti-human ACE2 polyclonal antibody (R&D Systems, Cat # AF933) or isotype goat polyclonal isotype control (R&D Systems ...
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bioRxiv - Immunology 2021Quote: ... and human IL4 (20 ng/ml; R&D Systems) were added immediately before experiments also as supplements ...
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bioRxiv - Immunology 2021Quote: ... 40ng/ml of rm M-CSF (R&D System) and allowed to differentiate for 7 days.
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bioRxiv - Immunology 2021Quote: ... IL-21 and IL-15 were measured in plasma diluted 1:2 from acute COVID-19 patients and acute healthy controls using a custom designed multiplex Magnetic Luminex Screening assay (R&D Systems), according to the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... and myeloperoxidase (MPO; R&D Systems) were incubated at 1:100 dilution in 10% donkey serum overnight at 4°C ...
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bioRxiv - Immunology 2021Quote: ... The cell-free supernatants derived from CD10neg/CD10pos neutrophils were added to CD4+ T-cells cultured in 96-well plates (8×104 cells/well) in the presence of neutralizing anti-IL-12 antibody (4μg/mL; MAB219, R&D Systems) or isotype control (4μg/mL ...
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bioRxiv - Immunology 2021Quote: ... and IL-1ß in the cell-culture supernatants were measured by ELISA (DIF50; R&D Systems) and using bead-based immunoassay (740929 ...
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bioRxiv - Immunology 2021Quote: ... or isotype control (4μg/mL; MAB002, R&D Systems). CD4+CD28null T-cells were cultured with cell-free supernatants derived from CD10neg neutrophils ...
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bioRxiv - Immunology 2021Quote: ... anti-human IL-17RC antibodies (R&D systems, Abingdon, UK) or universal negative control mouse (Dako ...
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bioRxiv - Immunology 2021Quote: ... 2 nM of recombinant CHIT1 (R&D Systems. Cat # 3559-GH-010) was incubated with 20 μM substrate in McIlvain buffer (100 mM citric acid ...
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bioRxiv - Immunology 2021Quote: ... Soluble uPAR was determined using Quantikine ELISA (R&D Systems, Minneapolis, MN, USA).
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bioRxiv - Immunology 2021Quote: ... All cytokines and neutralizing antibodies used in the study were purchased from R&D Systems unless otherwise stated ...
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bioRxiv - Immunology 2021Quote: ... Cells were stained with fluorescently-labeled antibodies directed against cell-specific surface marker for 30 min one ice in the dark (anti-mouse CD45-APC/Cy7, #103116, BioLegend; anti-mouse CD45-FITC, #FAB114F, R&D Systems, Wiesbaden-Nordenstadt ...
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bioRxiv - Immunology 2021Quote: ... and isotype control (mouse IgG1κ, both R&D Systems, Minneapolis, MN, USA). The cells were analysed in FACSCantoII (BD ...
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bioRxiv - Immunology 2021Quote: ... and stained with anti-Survivin (91630, R&D Systems) and isotype control (mouse IgG1κ ...
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bioRxiv - Immunology 2021Quote: ... 109-006-127), 250ng/ml human recombinant MEGACD40L (Enzo, ALX-522-110) and 20ng/ml Recombinant human IL-4 (R&D system, 204-IL-010). Cells were collected at specific time points for analysis.
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bioRxiv - Immunology 2021Quote: ... Soluble recombinant TLR2 was purchased from R&D systems. Anti-TLR2 and anti-TLR4 monoclonal antibodies were obtained from eBioscience ...
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bioRxiv - Immunology 2021Quote: ... 100 μL of recombinant soluble TLR2 (R&D systems) at 1 μg / ml are coated during 2 hours at room temperature in 96-well plates ...
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bioRxiv - Immunology 2021Quote: ... supernatants were harvested and stocked at −20°C until CXCL8 quantification by ELISA kits according the instructions of the manufacturer (R&D system).
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bioRxiv - Immunology 2021Quote: ... and 10% FBS (R&D Systems). MDA-MB-231 breast cancer cells were grown in DMEM (Life Technologies ...
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bioRxiv - Immunology 2021Quote: ... cells were stained with Annexin V (R&D Systems) per manufacturer’s instructions and were run immediately without fixation or were fixed in 1% PFA diluted in Annexin V binding buffer ...
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bioRxiv - Immunology 2021Quote: ... 50 ng/mL VEGF (R&D System), 50 ng/mL bFGF (PeproTech) ...
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bioRxiv - Immunology 2021Quote: ... The following antibodies were used: BRACHYURY (R&D System, IC2085A), APLNR (R&D System ...
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bioRxiv - Immunology 2021Quote: ... APLNR (R&D System, FAB8561A-025), CD3 (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... and mouse CCL19 DuoSet ELISA (R&D Systems) as per manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... ERAP2 (AF3830, R&D Systems), S-antigen (α-mGFP ...
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bioRxiv - Immunology 2021Quote: ... Membranes were blocked in 5% nonfat dry milk in TBST and probed overnight at 4°C with antibodies recognizing ERAP1 (AF2334, R&D Systems), ERAP2 (AF3830 ...
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bioRxiv - Immunology 2021Quote: ... Low potency TGFβ (R&D Systems) was used instead for Treg differentiation as indicated ...
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bioRxiv - Immunology 2021Quote: ... The membranes were then washed with PBST and incubated at 4°C overnight with goat anti-mouse IL-1β (250 ng ml−1; R&D Systems, AF-401-NA), mouse anti-mouse NLRP3 (1 μg ml−1 ...
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bioRxiv - Immunology 2021Quote: ... L-SIGN-hFc tag was purchased from R&D systems (USA). Antibody 4A8 was produced in-house according to GenBank accession no ...
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bioRxiv - Immunology 2021Quote: Mice aged 8-10 weeks were given 0.75 μg/g body weight αCCL11 and αCCL24 (R&D Systems; MAB420 and MAB528) or an equivalent amount of rat IgG (Sigma ...
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bioRxiv - Immunology 2021Quote: ... To detect CCR2 (475301; R&D Systems) and CCR3 (J073E5 ...
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bioRxiv - Immunology 2021Quote: ... The enzymatic reaction was stopped by adding 2N H2SO4 (R&D Systems) to each well and the plates read at 450 nm using a Multiscan FC Plate Reader (Thermo Scientific) ...
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bioRxiv - Immunology 2021Quote: ... anti-IL-4 (1 μg/ml) R&D Systems Cat# MAB204) and anti-IFN-γ (1 μg/ml R&D Systems Cat#MAB-285). Differentiation of Th17 cells was confirmed by measuring IL-17 expression by quantitative real-time PCR ...