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• Molecular architecture determines brain delivery of a transferrin-receptor targeted lysosomal enzyme

  Annie Arguello, et al., bioRxiv - Neuroscience 2021

  Quote: ... a biotinylated anti-IDS antibody (R&D Systems, Cat # BAF2449) was added to bind the immobilized ETV:IDS and IgG:IDS ...
• Semaphorin3F Drives Dendritic Spine Pruning through Rho-GTPase Signaling

  Bryce W. Duncan, et al., bioRxiv - Neuroscience 2021

  Quote: ... Western blotting was carried out using mouse monoclonal antibody clone M2 directed against the FLAG-tag on Npn2 (SigmaAldrich #F3165) or rabbit anti-NrCAM antibody (R&D Systems AF8538) and Avansta enhanced chemiluminescence ...
• Surf4 Promotes Endoplasmic Reticulum Exit of the Lysosomal Prosaposin-Progranulin Complex

  S. Devireddy, S.M. Ferguson, bioRxiv - Cell Biology 2021

  Quote: ... goat anti-cathepsin D (R&D systems; Catalog no # AF1014), sheep anti-mouse progranulin (R&D systems ...
• Ventricular, atrial and outflow tract heart progenitors arise from spatially and molecularly distinct regions of the primitive streak

  Kenzo Ivanovitch, et al., bioRxiv - Developmental Biology 2021

  Quote: ... rat anti-CER1 (R&D Systems, MAB1986), goat anti-T (1:250 ...
• Ventricular, atrial and outflow tract heart progenitors arise from spatially and molecularly distinct regions of the primitive streak

  Kenzo Ivanovitch, et al., bioRxiv - Developmental Biology 2021

  Quote: ... goat anti-DKK1 (R&D Systems, AF1765), rabbit ranti-Aldh1a2 (Abcam ab96060) ...
• PROTECTIVE ROLE FOR SMOOTH MUSCLE CELL HEPCIDIN IN ABDOMINAL AORTIC ANEURYSM

  P Loick, et al., bioRxiv - Physiology 2021

  Quote: ... Lipocalin (LCN2)-neutralising antibody MAB1857 (R&D systems) was infused intravenously at 4mg/Kg.
• PROTECTIVE ROLE FOR SMOOTH MUSCLE CELL HEPCIDIN IN ABDOMINAL AORTIC ANEURYSM

  P Loick, et al., bioRxiv - Physiology 2021

  Quote: Human HAMP was measured in plasma by ELISA DHP250 (R&D Systems). Mouse HAMP was measured in plasma by ELISA LS-F11620 (Ls-Bio).
• An integrated in silico-in vitro approach for identification of therapeutic drug targets for osteoarthritis

  Raphaëlle Lesage, et al., bioRxiv - Systems Biology 2021

  Quote: ... cells were differentiated for 14 days with or without Ihh supplement (150ng/ml, R&D Systems Europe LTD). The cells were harvested for RNA isolation on day 0 ...
• An integrated in silico-in vitro approach for identification of therapeutic drug targets for osteoarthritis

  Raphaëlle Lesage, et al., bioRxiv - Systems Biology 2021

  Quote: ... Recombinant Mouse IGF-I/IGF-1 Protein (10ng/ml, R&D Systems), Transforming Growth Factor (TGF)β1 (10ng/ml ...
• An integrated in silico-in vitro approach for identification of therapeutic drug targets for osteoarthritis

  Raphaëlle Lesage, et al., bioRxiv - Systems Biology 2021

  Quote: ... 10 ng/ml transforming growth factor beta one (TGFβ1, recombinant human, R&D systems) 25 μg/mL l-ascorbic acid 2-phosphate (Sigma Aldrich) ...
• Pseudohypoxic HIF pathway activation dysregulates collagen structure-function in human lung fibrosis

  Christopher Brereton, et al., bioRxiv - Cell Biology 2021

  Quote: ... TGFβ1 (R&D systems, 240-GMP-010, 10ng/mL), Dimethyloxaloylglycine (DMOG ...
• Unexpected arabinosylation after humanization of plant protein N-glycosylation

  Lennard L. Bohlender, et al., bioRxiv - Bioengineering 2021

  Quote: ... anti-hEPO monoclonal antibody (MAB2871; R&D Systems, Minneapolis, USA) and peroxidase-linked anti-mouse secondary antibody (NA 9310V ...
• SHED-dependent oncogenic signalling of the PEAK3 pseudo-kinase

  Youcef Ounoughene, et al., bioRxiv - Cancer Biology 2021

  Quote: The Proteome Profiler Human Phospho-Kinase Array Kit was purchased from R&D Systems. THP1 cells were lysed ...
• Host-pathogen genetic interactions underlie tuberculosis susceptibility

  Clare M. Smith, et al., bioRxiv - Microbiology 2021

  Quote: ... Cytokines and chemokines were assayed from organ homogenates using the pro-inflammatory focused 32-plex (Eve Technologies, Calgary, CA) or IFNγ DuoSet ELISA (R&D Systems) according to manufacturer protocol ...
• Aberrant FGF signaling promotes granule neuron precursor expansion in SHH subgroup infantile medulloblastoma

  Odessa R. Yabut, et al., bioRxiv - Cancer Biology 2021

  Quote: ... goat anti-Gli2 (1:1000; R&D Systems), rabbit anti-Gli3 (1:100 ...
• Mitochondrial ATP production is required for endothelial cell control of vascular tone

  Calum Wilson, et al., bioRxiv - Physiology 2021

  Quote: ... Cells were then incubated overnight at 4°C with goat anti-mouse platelet endothelial cell adhesion molecule (CD31/PECAM-1) primary antibody (R&D Systems cat. No. AF3628) diluted in antibody buffer (1:1000 ...
• Loss of p16INK4a in neuroblastoma cells induces shift to an immature state with mesenchymal characteristics and increases sensitivity to EGFR inhibitors

  Nil A. Schubert, et al., bioRxiv - Cancer Biology 2021

  Quote: Primary antibodies were p16INK4a (AF5779, R&D Systems, 1:200), MDM2 (A2716 ...
• PDFGRα⁺ Stromal Cells Promote Salivary Gland Proacinar Differentiation Through FGF2-dependent BMP7 Signaling

  Nicholas Moskwa, et al., bioRxiv - Developmental Biology 2021

  Quote: ... PDGFRα (1:100; R&D Systems #AF1062-SP), PDGFRβ (1:200 ...
• Notch signaling determines cell-fate specification of the two main types of vomeronasal neurons of rodents

  Raghu Ram Katreddi, et al., bioRxiv - Developmental Biology 2021

  Quote: ... *Goat anti-NeuroD1 (1:500, AF2746, R&D Systems), *Rabbit anti-Notch1 (1:50 ...
• Amino acids 484 and 494 of SARS-CoV-2 spike are hotspots of immune evasion affecting antibody but not ACE2 binding

  Marta Alenquer, et al., bioRxiv - Immunology 2021

  Quote: ... cells were stained with a primary antibody against ACE2 (4µg/ml, R&D Systems, catalog no. AF933) followed by a secondary antibody labelled with Alexa Fluor 568 (1:1000 ...
• IL-31 uncouples skin inflammation from itch sensation in allergic dermatitis

  Marlys S. Fassett, et al., bioRxiv - Immunology 2021

  Quote: ... Cells were sequentially challenged by addition of the following agonist solutions: SLIGRL (100 μM; R&D Systems), then either rmIL-31 (300 nM ...
• NADPH Oxidase 2 Derived Reactive Oxygen Species Promote CD8⁺ T cell Effector Function

  Jing Chen, et al., bioRxiv - Immunology 2021

  Quote: ... and stained for p-S6K-PE (clone #215247, R&D Systems), Perforin-APC (S16009A ...
• Kinetics Of Interferon-λ And Receptor Expression In Response To In Vitro Respiratory Viral Infection

  Alexey A Lozhkov, et al., bioRxiv - Immunology 2021

  Quote: ... Lambda IFN levels were evaluated by human IL-29/IL-28B (IFN-lambda 1/3) DuoSet ELISA (DY1598B, R&D Systems, USA). IL-6 was measured by human IL-6 DuoSet ELISA (DY206 ...
• Connexin Hemichannels with Prostaglandin Release in Anabolic Function of Bone to Mechanical Loading

  Dezhi Zhao, et al., bioRxiv - Physiology 2021

  Quote: ... and then probed with an anti-sclerostin (AF1589, 1:400, R&D systems, Minneapolis, MN, USA), anti-COX-2 (12375-1-AP ...
• Th17 immunity in the colon is controlled by two novel subsets of colon-specific mononuclear phagocytes

  Hsin-I Huang, et al., bioRxiv - Immunology 2021

  Quote: ... IL-17 and IFNγ in supernatants were assayed by ELISA (Biolegend or R&D systems). For data output cell number and ELISA were normalized per 1,000 of each APC subset used for co-culture.
• A multifaceted role of progranulin in regulating amyloid-beta dynamics and responses

  Huan Du, et al., bioRxiv - Neuroscience 2021

  Quote: ... goat anti-GPNMB (R&D Systems, AF2330), mouse anti-GAPDH (Proteintech Group ...
• SerpinB3 drives cancer stem cell survival in glioblastoma

  Adam Lauko, et al., bioRxiv - Cancer Biology 2021

  Quote: ... 20 ng/ml human (h)EGF and 20 ng/ml hFGF2 (R&D systems). Subsequently ...
• CXCL17 is an endogenous inhibitor of CXCR4 via a novel mechanism of action

  Carl W. White, et al., bioRxiv - Pharmacology and Toxicology 2021

  Quote: ... VEGF165a was purchased from R&D Systems and synthesis of fluorescent VEGF165a-tetramethylrhodamine (TMR ...
• Gene signatures and host-parasite interactions revealed by dual single-cell profiling of Plasmodium vivax liver infection

  Liliana Mancio-Silva, et al., bioRxiv - Microbiology 2021

  Quote: ... beta (8499-IF-010) and gamma (285-IF-100) (all purchased from R&D Systems). Cytokine units tested are given in the figures ...
• Interleukin-1 is overexpressed in injured muscles following spinal cord injury and promotes neurogenic heterotopic ossification

  Hsu-Wen Tseng, et al., bioRxiv - Physiology 2021

  Quote: ... and biotinylated goat IgG (BAF108, R&D Systems). Cells were washed and incubated 30 min with Streptavidin-APC/Cy7 and the viability dye 7-aminoactinomycin D (7-AAD ...
• Interleukin-1 is overexpressed in injured muscles following spinal cord injury and promotes neurogenic heterotopic ossification

  Hsu-Wen Tseng, et al., bioRxiv - Physiology 2021

  Quote: ... IL-1β and IL-1α concentrations in conditioned media were evaluated using Human IL1-β and IL-1α ELISA assays (DY201 and DY200, R&D Systems) according to the manufacturer’s instructions.
• Interleukin-1 is overexpressed in injured muscles following spinal cord injury and promotes neurogenic heterotopic ossification

  Hsu-Wen Tseng, et al., bioRxiv - Physiology 2021

  Quote: ... 1% P/S antibiotics and 10 ng/ml basic fibroblast growth factor (bFGF) (R&D Systems). Human MPCs were trypsinized and incubated 30 min with biotinylated anti-human PDGFRα (BAF322 R&D Systems ...
• Interleukin-1 is overexpressed in injured muscles following spinal cord injury and promotes neurogenic heterotopic ossification

  Hsu-Wen Tseng, et al., bioRxiv - Physiology 2021

  Quote: ... Human MPCs were trypsinized and incubated 30 min with biotinylated anti-human PDGFRα (BAF322 R&D Systems) goat polyclonal antibody and CD56-PE (clone B159 ...
• Interleukin-1 is overexpressed in injured muscles following spinal cord injury and promotes neurogenic heterotopic ossification

  Hsu-Wen Tseng, et al., bioRxiv - Physiology 2021

  Quote: ... IL-1β plasma level was evaluated using Human IL1-β ELISA Kit (DLB50, R&D Systems) according to the manufacturer procedure.
• Interleukin-1 is overexpressed in injured muscles following spinal cord injury and promotes neurogenic heterotopic ossification

  Hsu-Wen Tseng, et al., bioRxiv - Physiology 2021

  Quote: ... Mouse IgG3 isotype (MAB007, R&D systems) and rabbit polyclonal isotype (ab37415 ...
• Skeletal dysplasia-causing TRPV4 mutations suppress the hypertrophic differentiation of human iPSC-derived chondrocytes

  Amanda R. Dicks, et al., bioRxiv - Developmental Biology 2021

  Quote: ... 10 ng/ml human transforming growth factor-β3 (TGFβ3; cat. num. 243-B3-010/CF; R&D Systems, Minneapolis, MN), 1 µM Wnt-C59 ...
• H3 acetylation selectively promotes basal progenitor proliferation and neocortex expansion by activating TRNP1 expression

  Cemil Kerimoglu, et al., bioRxiv - Developmental Biology 2021

  Quote: ... SOX2 mouse mAb (1:100; R&D Systems), SOX2 goat (1:100 ...
• Novel roles of small extracellular vesicles in regulating the quiescence and proliferation of neural stem cells

  Jingtian Zhang, et al., bioRxiv - Developmental Biology 2021

  Quote: ... and N-2 Plus supplement (R&D Systems) in DMEM/F-12 (Gibco ...
• Novel roles of small extracellular vesicles in regulating the quiescence and proliferation of neural stem cells

  Jingtian Zhang, et al., bioRxiv - Developmental Biology 2021

  Quote: ... Quiescence was induced with quiescent medium [50 ng/mL BMP4 (R&D Systems) in proliferation medium minus EGF] after two washings with phosphate-buffered saline (PBS ...
• Role of YAP in early ectodermal specification and a Huntington’s Disease model of human neurulation

  Francesco M. Piccolo, et al., bioRxiv - Developmental Biology 2021

  Quote: SOX10 (R&D Systems AF2864)
• Human induced pluripotent stem cell-derived three-dimensional cardiomyocyte tissues ameliorate the rat ischemic myocardium by remodeling the extracellular matrix and cardiac protein phenotype

  Junya Yokoyama, et al., bioRxiv - Cell Biology 2021

  Quote: ... and vascular endothelial growth factor (VEGF; R&D Systems, MN, USA), and small molecules such as IWR-1 (Wako) ...
• A Cryptic K48 Ubiquitin Chain Binding Site on UCH37 is Required for its Role in Proteasomal Degradation

  Jiale Du, et al., bioRxiv - Biochemistry 2021

  Quote: Anti PSMB7 (R&D Systems, Cat. #MAB7590)
• Paraxial mesoderm organoids model development of human somites

  Christoph Budjan, et al., bioRxiv - Developmental Biology 2021

  Quote: ... Organoids were subsequently differentiated towards cartilage by culturing them in the presence of 20 ng/ml BMP4 (R&D Systems, cat. no. 314-BP-010) for 6 days.
• Two distinct classes of co-chaperones compete for the EEVD motif in heat shock protein 70 (Hsp70) to tune its activity

  Oleta T. Johnson, et al., bioRxiv - Biochemistry 2021

  Quote: ... + UbcH5c/UBE2D3 (R&D Systems) (400 nM Ube1 and 4 μM UbcH5c) ...
• Two distinct classes of co-chaperones compete for the EEVD motif in heat shock protein 70 (Hsp70) to tune its activity

  Oleta T. Johnson, et al., bioRxiv - Biochemistry 2021

  Quote: Four 4× stock solutions were prepared containing (i) Ube1 (R&D Systems) + UbcH5c/UBE2D3 (R&D Systems ...
• Two distinct classes of co-chaperones compete for the EEVD motif in heat shock protein 70 (Hsp70) to tune its activity

  Oleta T. Johnson, et al., bioRxiv - Biochemistry 2021

  Quote: ... Ubiquitin (R&D Systems) (1 mM Ub) ...
• Ubr1-induced selective endo-phagy/autophagy protects against the endosomal and Ca²⁺-induced proteostasis disease stress

  Ben B. Wang, et al., bioRxiv - Biochemistry 2021

  Quote: ... Selective isolation of MLC1-complex from the PM was achieved by cs-IP using anti-GlialCAM Ab (1:2000, R&D Systems). Ab was bound on ice to live cells for 45min ...
• Lineage and Spatial Mapping of Glioblastoma-associated Immunity

  Vidhya M. Ravi, et al., bioRxiv - Cancer Biology 2021

  Quote: ... the neutralizing antibody anti-IL10 hAB (R&D systems) were added to the cells at the concentration of 5 μg/ml.
• Cell-surface tethered promiscuous biotinylators enable small-scale surface proteomics of human exosomes

  Lisa L. Kirkemo, et al., bioRxiv - Biochemistry 2021

  Quote: ... ANPEP (R&D Systems, AF3815), vimentin (Cell Signaling Technology ...
• Cell-surface tethered promiscuous biotinylators enable small-scale surface proteomics of human exosomes

  Lisa L. Kirkemo, et al., bioRxiv - Biochemistry 2021

  Quote: ... Primary antibodies used were ANPEP (R&D Systems, AF3815), FN1 (Abcam ...