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Citations for Santa Cruz :
1 - 50 of 57 citations for rno mir 125b 5p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2019Quote: ... MART1and PFK transcripts were analyzed by SYBR Green–based real-time quantitative RT-PCR (qRT-PCR) using specific primers purchased from Santa Cruz. Data were normalized to the expression of RPL19 and are expressed as fold changes.
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bioRxiv - Molecular Biology 2020Quote: ... Pol II Ser 5P+ (Santa Cruz), H3K4me3(Active motif 39159) ...
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bioRxiv - Neuroscience 2022Quote: ... We used UltraCruz Hard-set Mounting Medium with DAPI (Santa Cruz, sc-359850) or Vectashield HardSet Mounting Medium with DAPI (Vector Laboratories ...
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bioRxiv - Molecular Biology 2021Quote: ... with another RT inhibitor BPPA (Santa Cruz Biotechnology Inc., CA) that specifically inhibits the catalytic RT domain of human telomerase (hTERT).
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bioRxiv - Developmental Biology 2023Quote: ... EMCN (Santa Cruz; clone V.5C7; 1:100; 2h at RT), COL10 (Abcam ...
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bioRxiv - Genomics 2020Quote: ... RT before washing and adding DAPI mounting media (Santa Cruz Biotechnology, Heidelberg, Germany) (Suppl ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... rotated at RT with 2μg Anti-BrdU antibody (Santa Cruz Biotech, IIB5, sc-32323) in BrU binding buffer for 10 min. ...
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bioRxiv - Physiology 2021Quote: ... membranes were incubated for 1 hour at RT with secondary antibodies (Santa Cruz Biotechnology, USA) and StrepTactin-AP Conjugate (Biorad ...
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bioRxiv - Neuroscience 2019Quote: ... RT with anti c-fos 1ry AB (1:500; rabbit; sc-52 Santa Cruz Biotechnology) in PBS with1% BSA and 0.1% Triton X-100 ...
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bioRxiv - Neuroscience 2023Quote: ... blots were incubated for 1 h at RT with primary antibody [GSK3α (sc-5264, Santa Cruz), GSK3β (9315S ...
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bioRxiv - Cancer Biology 2023Quote: ... RT) and stained with the following primary antibodies: Lamp-1 (rat; 1:400; Santa Cruz Biotechnology), p62 MBL (rabbit ...
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bioRxiv - Plant Biology 2021Quote: ... incubated at RT for 1 hr with secondary antibodies conjugated with horseradish peroxidase (HRP) (Santa Cruz Biotech). Rinsed membranes were then treated with ECL Prime solution (GE Healthcare ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... was followed by HRP-conjugated mouse IgG kappa binding protein (1:5000, #516102, Santa Cruz; 2 h, RT). Finally ...
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bioRxiv - Cancer Biology 2020Quote: ... for an hour at RT and incubating with KDM2B antibody (sc-293279, 1:50, Santa Cruz Biotechnology, CA, USA) overnight at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... The following primary antibodies were used for 1h at RT: VE-cadherin F8 (Santa Cruz, SC-9989, 1:100), ERG (Abcam ...
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bioRxiv - Molecular Biology 2022Quote: ... it was incubated for 1 h at RT with species-specific horseradish peroxidase (HRP)-coupled secondary antibody (Santa Cruz, sc-2005 ...
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bioRxiv - Neuroscience 2020Quote: ... The following primer antibodies were used: SHIP1/INPP5D (CST #4C8 1:500, Rabbit mAb) and GAPDH (Santa Cruz #sc-32233). Each sample was normalized to GAPDH ...
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bioRxiv - Cell Biology 2019Quote: ... the salivary glands were incubated 30 min at RT with 10 μM VO-OHpic trihydrate (Santa Cruz Biotechnology sc-216061). DMSO was used as vehicle and its final concentration was 0.25 μL/mL in Grace’s medium ...
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bioRxiv - Neuroscience 2019Quote: ... incubated for 1 hour at RT with an HRP-conjugated β-actin antibody at 1/5000 (Santa Cruz #sc-47778) in 5% blotto and developed as previously described ...
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bioRxiv - Physiology 2022Quote: ... blocked with 5% BSA in PBS 0.2% Tween for 1 hr RT and probed with anti-aquaporin E2 FITC conjugated sc-515770 (Santa Cruz) 1:100 overnight ...
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bioRxiv - Molecular Biology 2019Quote: ... solution and incubated for 1.5 h RT or overnight at 4° C with the primary antibodies in milk PBS-T (RNAP1: anti-RPA194, sc-48385, Santa Cruz; ACTβ ...
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bioRxiv - Biochemistry 2020Quote: ... before blocking for 1 h with blocking buffer [5% goat-serum in PBS-T (PBS + 0.1% Triton-x100)] at RT and were then incubated with acetylated α-tubulin antibody (1:300, Santa Cruz Biotechnology ...
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bioRxiv - Microbiology 2020Quote: ... followed by 2 h incubation at RT with the primary antibodies (anti-biotin 1:1000 Bethyl - and anti-ICP4 H943 1:250 Santa Cruz Biotechnology ...
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bioRxiv - Developmental Biology 2021Quote: ... Primary and secondary antibodies were diluted per manufacturer recommended concentrations in 10%FBS/0.3% Triton X-100/DPBS and incubated for 1 hour at RT using the following antibodies Tbx3 (sc-17871 Santa Cruz); Nr5a2 (PP-H2325-00 R&D Systems) ...
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bioRxiv - Genetics 2019Quote: ... and incubated in horseradish peroxidase-conjugated secondary antibody in 1% (w/v) skimmed milk in PBS at the following concentrations for 30-120 min at RT (anti-goat, Santa Cruz sc-2020 ...
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bioRxiv - Neuroscience 2022Quote: ... They were again washed 3 times in DPBS (10 minutes at RT) and then mounted with UltraCruz mounting medium (Santa Cruz) for imaging.
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bioRxiv - Biophysics 2023Quote: ... for 15 min at RT prior to saturation with primary mouse anti-lamin A/C (1:1000, sc-376248, Santa Cruz) or anti-α-tubulin (1:1000 ...
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bioRxiv - Cancer Biology 2023Quote: ... The probing was done either for 1h at RT or o/n at +4°C using following antibodies: rabbit anti-Notch3 (M-143, Santa Cruz) or mouse anti-β-actin (A1978 ...
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bioRxiv - Bioengineering 2020Quote: ... Membranes were blocked using 5% skimmed milk for 2 hours at room temperature (RT) following which the blots were incubated overnight at 4 °C in anti-VCAM-1 (Santa Cruz, CA), anti-ICAM-1 (Santa Cruz ...
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bioRxiv - Neuroscience 2020Quote: ... incubated for 1 hr at RT with an HRP-conjugated mouse anti-β-actin antibody (1:5000, Santa Cruz Biotechnology #sc-47778) in 5% BLOTTO and developed as previously described ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were blocked in 5% milk in TBS with 0.05% Tween-20 for 1 h at RT before addition of primary antibodies (SRRM4, HPA052783, Sigma-Aldrich Quimica S.A., Madrid, Spain; GAPDH, sc-47724, Santa Cruz Biotechnology) diluted 1:1,000 in blocking buffer ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were fixed with 3.7% formaldehyde for 9 min at RT and permeabilized with acetone for 5 min at −20C and stained with c-Myc (9E10, Santa Cruz Biotechnology) and eIF4G (H-300 ...
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bioRxiv - Immunology 2020Quote: ... Diluted phage preparations were added and allowed to incubate for 1 hour at RT before captured virions were detected using a 1:5000 dilution of anti-M13-HRP (Santa Cruz Biotechnology) for 1 hour at RT ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were incubated for 1 h at RT with anti-GluN1 antibody (1:200, Alomone) and anti-NOS1 antibody (1:50, Santa Cruz Biotechnology) in the blocking solution ...
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bioRxiv - Pathology 2021Quote: ... Membranes were washed three times with TBS-T and incubated for 1 hour at RT with the secondary antibody anti-rabbit antibody diluted 1:5000 (Santa Cruz Biotechnology). Blots were developed with ECL (ECL Prime ...
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bioRxiv - Cell Biology 2021Quote: ... Slides were then incubated with blocking solution (1% BSA and 10% Donkey serum in freshly prepared 1X PBS) for 2 hrs at RT and then subsequently incubated with primary antibodies: MAP1B at 1:100 (N-19, Santa Cruz Biotechnology), Kif3a at 1:300 (Abcam ...
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bioRxiv - Molecular Biology 2022Quote: ... it was incubated for 1 h at RT with species-specific horseradish peroxidase (HRP)-coupled secondary antibody (Santa Cruz, sc-2005, goat anti-mouse; Santa Cruz, sc-2357 ...
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bioRxiv - Immunology 2023Quote: ... Diluted phage preparations were added and allowed to incubate for 1 hour at RT before captured virions were detected using a 1:5000 dilution of anti-M13-HRP (Santa Cruz Biotechnology) for 1 hour at RT ...
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bioRxiv - Neuroscience 2019Quote: ... in PBS for 20 min at room temperature (RT) and incubated with rabbit polyclonal antibodies against ATF3 (Santa Cruz, 1:500, RRID: AB_1078233), and Horseradish peroxidase (HRP)-conjugated mouse monoclonal antibody against GAPDH (Cell Signaling ...
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bioRxiv - Microbiology 2020Quote: ... in PBS–1% BSA 1 h RT and 1:400 anti-goat conjugated with CFL 488 secondary antibody (green) (SC362255, Santa Cruz Biotechnologies, USA) in PBS–1% BSA for 1 h RT ...
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bioRxiv - Molecular Biology 2022Quote: ... and then incubated for 1.5-2h h at room temperature (RT) with anti-FUS (Santa Cruz, 4H11, 1:3000 dilution in PBS-T), home-made polyclonal rabbit anti-FUS antibody (9) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Membranes were blocked for 3h at RT and incubated with primary antibodies (rabbit anti-mouse NF-κB(RelA/p65/) (sc-8008; Santa Cruz Biotechnology, Santa Cruz, CA), rabbit anti-mouse phospho(p)-RelA/p65(NF-κB ...
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bioRxiv - Biochemistry 2023Quote: ... The mixture was incubated for 1 hour with agitation at RT and then transferred to high-binding microplate strips (Santa Cruz Biotechnology Inc., Santa Cruz, CA) previously coated with 0.5 µg of each sonicated vaccine candidate in coating buffer (PBS) ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were incubated for 1 hour at RT or overnight at 4°C with the following antibodies: mouse anti-HuR (Santa Cruz, sc-71290) and mouse anti-βACTIN antibody (3700 ...
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bioRxiv - Cell Biology 2024Quote: ... samples were incubated at RT with primary antibodies (RNA Polymerase II, rabbit polyclonal, 1:1000, Abcam, ab5131; Treacle, mouse monoclonal, 1:500, Santa Cruz, sc-374536) for 1 hour ...
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bioRxiv - Cell Biology 2024Quote: ... 0.2% Triton X-100 in 1XTBS) for 1 h at RT followed by incubation with primary antibodies against ERG (Santa Cruz, 1:100) for 72 h and EC marker ...
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bioRxiv - Cell Biology 2019Quote: ... Blots were blocked with 5% milk in TBST (TBS + 0.1% Tween 20) for 1 h at RT and incubated with GFP antibody (1:1000, sc-9996, Santa Cruz Biotechnology, Dallas, TX, USA) or TDRKH antibody (1:1000 ...
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bioRxiv - Immunology 2023Quote: ... and allowed to incubate for 1 hour at RT before captured virions were detected using a 1:5000 dilution of anti-M13-HRP (Santa Cruz Biotechnology, Dallas, TX) for 1 hour at RT ...
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bioRxiv - Cell Biology 2021Quote: ... was diluted 1:10000 in 5% non-fat dry milk in PBST1X and incubated 1h at RT and subsequently with Goat anti-mouse-HRP (Santa Cruz, Dallas, TX, USA-A1303) diluted 1:1000 in 2% non-fat dry milk in PBST1X 30min RT.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Membranes were blocked for 3h at RT and incubated with primary antibodies (rabbit anti-mouse NF-κB(RelA/p65/) (sc-8008; Santa Cruz Biotechnology, Santa Cruz, CA), rabbit anti-mouse phospho(p)-RelA/p65(NF-κB ...