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Citations for Santa Cruz :
1 - 50 of 901 citations for Stem Cell Culture Media since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... 5×104 cells were cultured in cell culture media with 5ug/ml Polybrene (Santa Cruz Biotechnology). Lentiviral particles were added to cells and incubated overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... The filtered viruses were added to culture media of cells with polybrene (Santa Cruz, final concentration of 8 µg/mL). Fresh media were replaced 48 hours after infection ...
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bioRxiv - Cell Biology 2023Quote: ... cells were seeded in microslide channels at a final concentration of ∼5×105 cells/ml in 120 µL cell culture media containing 40 μM BrdU:BrdC mix (3:1, Cat.# B5002, Sigma and Cat.# 284555, Santa Cruz Biotec) and grown for 17-24 h ...
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bioRxiv - Genomics 2020Quote: ... the media was replaced with fresh culture media and the cells were allowed to recover for 48 hours before beginning puromycin selection (Santa Cruz Biotechnology #SC-108071). Cells were treated with 800ng/ml puromycin for a total of 72 hours ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... embryos were incubated with a mouse monoclonal antibody against Oct4 stem cell marker (sc-5279, Santa Cruz Biotechnology), followed by an incubation with a Alexa Fluor™ 488 donkey anti-mouse IgG secondary antibody (A-21202 ...
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bioRxiv - Molecular Biology 2022Quote: ... Rapamycin (sc-3054) for cell culture was purchased from Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2023Quote: ... ReN cells were infected by lentiviruses in media supplemented with Polybrene (Santa Cruz Biotechnology) with the final concentration of 8 μg/ml ...
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bioRxiv - Biochemistry 2019Quote: ... media was changed to media containing 2 μg/mL puromycin (Santa Cruz). Following selection ...
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bioRxiv - Cancer Biology 2021Quote: Following cell culture supplements were used: 2-Deoxy-D-glucose (2DG) (Santa Cruz Biotechnology), D-Glucose (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: ... Primary antibodies used for immunofluorescent staining in cell culture were Actin (sc-8432; Santa Cruz), MURC (HPA021021 ...
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Cholesterol deprivation induces TGFβ signaling to promote basal differentiation in pancreatic cancerbioRxiv - Cancer Biology 2019Quote: ... cells were treated with media containing LDS or LDS with 1uM Compactin (#sc-200853, Santa Cruz). RNA and protein lysates were collected in 48 hours after treatment.
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bioRxiv - Cancer Biology 2023Quote: ... Cell lines were then transduced by adding viral particles to media containing polybrene (Santa Cruz Biotechnology) at a concentration of 10 μg/mL and selected with an appropriate antibiotic 24 to 48 hours after transduction to generate cells stably expressing transgene or shRNA.
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bioRxiv - Cancer Biology 2023Quote: ... by culturing cells in lentiviral media plus 10 ug/mL Polybrene (#sc-134220, Santa Cruz Biotechnology). After 24 hours ...
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bioRxiv - Neuroscience 2023Quote: ... fixed cell cultures were incubated with monoclonal antibodies against ELAVL4 (mouse IgG, 1:200, Santa Cruz), NeuroD1 (mouse IgG ...
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bioRxiv - Bioengineering 2019Quote: Cells were cultured in EN media with 10 μM ROCK inhibitor (Y-27632, Santa Cruz, sc-281642A). The presence of ROCK inhibitor for at least 24 h was critical for cell survival after electroporation ...
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bioRxiv - Cancer Biology 2022Quote: ... HAS overexpressing cells were cultured in their respective medias containing 1 μg/mL doxycycline (Santa Cruz Biotechnology). For glycolytic inhibition studies ...
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bioRxiv - Microbiology 2021Quote: ... HTLV-1 Gag expression in cells and VLPs in the cell culture supernatant was detected using a mouse monoclonal antibody HTLV-1 p24 (6G9) (Santa Cruz Biotechnology, TX) 1:3,000 and secondary antibody goat anti-mouse IgG StarBright Blue 700 (Bio-Rad ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were transduced by addition of viral media in the presence of 8 ug/mL polybrene (Santa Cruz Biotechnology), centrifuged at 1178 x g for 30 minutes at 37C ...
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bioRxiv - Cancer Biology 2023Quote: ... media was removed and cells incubated with 20 mL of PBS containing 2 mM disuccinimidyl glutarate (DSG) (Santa Cruz Chemical) for 20 minutes at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... were added to the cell culture at an MOI of 0.25 in the presence of polybrene (Santa Cruz, Dallas, Texas). At 24 hours post-transduction ...
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bioRxiv - Cancer Biology 2024Quote: ... MCF10A-derived 1E7 cells cultured on tissue culture polystyrene were induced with 1 μg/mL doxycycline (204734, Santa Cruz Biotechnology) for 24 hours before being collected with 0.05% trypsin-EDTA.
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bioRxiv - Pathology 2020Quote: ... Transfected cells were selected by selection media containing 2 μg/ml puromycin dihydrochloride (cat. no. sc-108071; Santa Cruz Biotechnology, Inc.). After confirming the decrease in LOXL2 expression by reverse transcription-quantitative polymerase chain reaction (RT-qPCR ...
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bioRxiv - Pathology 2020Quote: ... HK-2 cells were treated with media containing 5 μg/ml of polybrene (cat. no. sc-134220; Santa Cruz Biotechnology, Inc.), and then LOXL2 shRNA lentiviral particles and control shRNA lentiviral particles of 1 and 2 multiplicity of infection (MOI ...
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bioRxiv - Cancer Biology 2022Quote: ... The HUVECs were incubated with conditioned media of control and knockdown cells with or without IgG and PEDF antibodies (Santa Cruz). The migration ability of the HUVECs was evaluated by measuring the gap widths at time intervals of 0 and 24 h ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were seeded in a 12-well plate at 2x106 cells per well in 1 mL standard media supplemented with 10 μg/mL polybrene (Santa Cruz). Lentivirus was placed in the wells ...
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bioRxiv - Genomics 2024Quote: ... lentiviral transduction was performed by mixing cells in media supplemented with 8 µg/mL of polybrene (Santa Cruz Biotechnology 28728-55-4) with lentivirus and centrifuging in cell culture plates at 1000 xg and 33 °C for 2 hours ...
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bioRxiv - Immunology 2023Quote: ... the 2X inhibitor media contained HAR (Santa Cruz Biotechnology) at 10μg/mL (final concentration 5μg/mL) ...
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bioRxiv - Microbiology 2020Quote: ... the cultures were monitored by smearing and selection done by changing the media and replacing with fresh media containing 100 nM pyrimethamine (Santa Cruz Biotechnology Inc). On day 4 post transfection the cultures were diluted 1/3 in 5ml fresh media containing 100 nM pyrimethamine and 100 µl erythrocytes ...
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bioRxiv - Microbiology 2020Quote: ... the cells were grown in 6-well tissue culture plates and transfected with 80 pmol EGFR siRNA (sc-29301, Santa Cruz Biotechnology), and EphA2 siRNA (sc-29304 ...
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bioRxiv - Neuroscience 2021Quote: HEK-293 cells in different culture formats were transfected with 3 target-specific siRNAs against human MAD2 (sc-35837, Santa Cruz Biotechnology) or siRNA against Luciferase (Dharmacon ...
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bioRxiv - Neuroscience 2022Quote: ... cell culture supernatants of cultured cerebellar neurons were incubated overnight with Reelin antibody G10 and Protein A/G beads (Santa Cruz Biotechnologies) or brain homogenates were resuspended in immunoprecipitation buffer (25mM Tris-HCl ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were prepared from culture cells (washed by cold PBS) or tissues by homogenization with a radioimmune precipitation assay lysis buffer (sc-24948, Santa Cruz Biotechnology) containing HaltTM protease and phosphatase inhibitor mixture (catalog no ...
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bioRxiv - Cancer Biology 2020Quote: ... Cas9-expressing cell lines were infected at a density of 2×105 cells in 1.5 mL media in the presence of 4 μg/mL polybrene (Santa Cruz Biotechnology, SC-134220). sgRNA resistant human ZRSR2 DNA fragment was synthesized by Twist Bioscience and cloned into pRRL.idTomato.
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bioRxiv - Genetics 2022Quote: ... Once the cells were ∼50% confluent (after 2-3 days) the media was changed to StemFlex with 1ug/uL polybrene (Santa Cruz SC-134220), and 10uL each of Ngn2 and rtTA virus was added to each well ...
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bioRxiv - Microbiology 2022Quote: The efficiency of immature particle production was analyzed by quantifying Gag proteins in released particles by harvesting cell culture supernatants by using immunoblot analysis with a mouse monoclonal anti-HIV-1 p24 antibody (Catalogue #: sc-69728; Santa Cruz Biotechnology, TX). Briefly ...
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bioRxiv - Cell Biology 2020Quote: For the generation of conditioned media tumor B-cells were incubated with 3 µM of mafosfamide sodium salt (Santa Cruz, Santa Cruz, CA, USA), 10 µM Nutlin-3A (Tocris Bioscience ...
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bioRxiv - Cell Biology 2020Quote: For the generation of conditioned media tumor B-cells were incubated with 3 µM of mafosfamide sodium salt (Santa Cruz, Santa Cruz, CA, USA), 10 µM Nutlin-3A (Tocris Bioscience ...
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bioRxiv - Biochemistry 2023Quote: ... Target cells were transduced in suspension with the filtered virus-containing media in the presence of 8 μg/mL polybrene (Santa Cruz Biotechnology, #sc-134220).
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bioRxiv - Molecular Biology 2023Quote: ... The efficiency of immature particle production was analyzed by quantifying Gag proteins in released particles through harvesting cell culture supernatants and by using immunoblot analysis with a mouse monoclonal anti-HTLV p24 antibody (Catalogue #: sc-53891; Santa Cruz Biotechnology, Dallas, TX). To determine particle production (i.e. ...
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bioRxiv - Molecular Biology 2024Quote: The efficiency of immature particle production was analyzed by quantifying Gag proteins in released particles through harvesting cell culture supernatants and by using immunoblot analysis with a mouse monoclonal anti-HTLV p24 antibody (Catalogue #: sc-53891; Santa Cruz Biotechnology, Dallas, TX). Briefly ...
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bioRxiv - Genomics 2021Quote: ... iPSC-derived MSCs were cultured to at least passage 6 and seeded at 4.2 × 104 cell/cm2 in culture dishes (4-10cm diameter) coated in 5ug/cm2 Type I Collagen (sc-136157, Santa Cruz Biotechnology, Dallas, TX, USA). After culturing cells for 1 day in MSC medium ...
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bioRxiv - Microbiology 2022Quote: ... cultures were treated with 20 µg/mL bicyclomycin (Santa Cruz Biotechnology) for 30 min at mid-log phase.
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bioRxiv - Bioengineering 2021Quote: ... gels were cultured in complete MCF10A media containing staurosporine (Santa Cruz Biotechnology) and left on a rocker plate at 0.33 Hz for 6 hrs to enhance diffusive transport.
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bioRxiv - Molecular Biology 2021Quote: ... the culture medium was replaced with transfection medium (Santa Cruz, #sc-36868) containing 6 μL of transfection reagent (Santa Cruz ...
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bioRxiv - Genomics 2020Quote: ... RT before washing and adding DAPI mounting media (Santa Cruz Biotechnology, Heidelberg, Germany) (Suppl ...
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bioRxiv - Cell Biology 2021Quote: ... sub-confluent primary cultures were dissociated with 0.5% trypsin-EDTA (Santa Cruz, USA) to single cell suspension and passaged at a density of 6 × 103 cells/cm2 ...
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bioRxiv - Cell Biology 2022Quote: ... HLMVECs were transduced with lentivirus in media containing 4ug/mL Polybrene (Santa Cruz Biotechnology), and expression was observed 2-4 days following infection.
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bioRxiv - Immunology 2022Quote: Apoptosis in FLS cultures was measured using a commercial flow cytometry kit (Santa Cruz biotechnology) for Annexin V-FITC binding to phosphatidylserine [28] ...
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bioRxiv - Biochemistry 2022Quote: ... the culture medium was changed to Neurobasal/B27 medium supplemented with 0.5mM dbcAMP (Santa cruz), 0.2 mM ascorbic acid (Stem cell technologies) ...
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bioRxiv - Cancer Biology 2023Quote: In vitro melanocyte culture was analyzed by immunocytochemistry using anti-MelCAM mAb (Santa Cruz Biotechnology) and Phospho-Paxillin (Tyr31 ...