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1 - 50 of 819 citations for Recombinant Zika virus NS1 Protein His Avi tagged Biotinylated since 2019

• The PELOTA-HBS1 Complex Orchestrates mRNA Translation Surveillance and PDK1-mediated Plant Growth and Development

  Wei Kong, et al., bioRxiv - Plant Biology 2021

  Quote: ... His-tagged proteins were detected by a mouse anti-His antibody (sc-8036, 1:3000, Santa Cruz Biotechnology). GST-tagged proteins were detected by a mouse anti-GST antibody (sc-138 ...
• LDL Receptor-Related Protein 5 is a Selective Transporter for Unesterified Polyunsaturated Fatty Acids

  Wenwen Tang, et al., bioRxiv - Cell Biology 2023

  Quote: ... Recombinant human IgG1-tagged proteins were then purified by the Protein A/G agarose (Santa Cruz Biotechnology). Proteins were eluted with 100 mM glycine pH 3.0 and collected in tubes containing 1 M Tris buffer ...
• A thermostable, closed, SARS-CoV-2 spike protein trimer

  Xiaoli Xiong, et al., bioRxiv - Biochemistry 2020

  Quote: ... Presence of His-tagged protein was detected by chemical luminescence using His-probe (H-3) HRP (sc-8036 HRP, Santa Cruz Biotechnology) monoclonal antibody horseradish peroxidase (HRP ...
• TRIM 25 nuclear translocation is hampered by localization to influenza A virus non-structural protein 1 cytosolic aggregates

  Anne Weiß, Ivan Haralampiev, Susann Kummer, bioRxiv - Microbiology 2022

  Quote: ... anti-NS1 (Santa Cruz Biotechnology), and anti-beta-actin (Sigma Aldrich ...
• Identifying cellular RNA-binding proteins during infection uncovers a role for MKRN2 in influenza mRNA trafficking

  Stefano Bonazza, et al., bioRxiv - Microbiology 2023

  Quote: ... NS1 (Santa Cruz; Sc-13056), YTHDF2 (Proteintech ...
• Melanomagenesis driven by a kinase-dead mutant BRAF lacking codons 600-618

  Megan A. Girtman, et al., bioRxiv - Cancer Biology 2021

  Quote: ... recombinant MEK1 protein (Santa Cruz Biotechnology, Dallas, TX), and incubated for 30 minutes at 30° C with gentle shaking ...
• Identification and characterization of interactions between Influenza A Virus NS1 protein and the human ubiquitin proteasome system

  Laurie-Anne Lamotte, et al., bioRxiv - Molecular Biology 2023

  Quote: ... the Influenza A NS1 Mouse Monoclonal Antibody (sc-130568, Santa Cruz Biotechnology), β-Tubulin Rabbit Polyclonal Antibody (10094-I-AP ...
• Specificity and mechanism of 1,6 hexanediol-induced disruption of nuclear transport

  Elizabeth C. Riquelme Barrientos, et al., bioRxiv - Molecular Biology 2023

  Quote: ... GFP-tagged proteins were detected with anti-GFP (Santa Cruz sc-9996 HRP) was used ...
• The human RNA polymerase I structure reveals an HMG-like transcription factor docking domain specific to metazoans

  Julia L. Daiß, et al., bioRxiv - Biochemistry 2021

  Quote: ... The tagged protein RPA1 was detected by the primary antibody (sc-48385, Santa Cruz Biotechnology), which was subsequently detected by the fluorescently labeled secondary antibody (926-32210 ...
• A potential function for the helicase Dbp5 in cytoplasmic quality control

  Luisa Querl, et al., bioRxiv - Cell Biology 2022

  Quote: ... MYC-tagged proteins with an anti-MYC antibody (sc-789; Santa Cruz, dilution 1:750). Secondary anti-rabbit IgG (H+L)-HRPO and anti-mouse IgG (H+L)-HRPO (Dianova ...
• CRISPR screens in sister chromatid cohesion defective cells reveal PAXIP1-PAGR1 as regulator of chromatin association of cohesin

  Janne J.M. van Schie, et al., bioRxiv - Molecular Biology 2022

  Quote: ... Flag-tagged PAGR1 was precipitated using Protein A/G Plus agarose beads (Santa Cruz; SC2003) with mouse-anti-Flag antibody (M2 ...
• Factors that affect protein abundance of the bZIP transcription factor ABRE-BINDING FACTOR 2 (ABF2), a positive regulator of abscisic acid signaling

  Katrina J Linden, et al., bioRxiv - Plant Biology 2021

  Quote: ... GST-tagged proteins were detected with rabbit polyclonal GST (Z-5): sc-459 (Santa Cruz Biotechnology) at a dilution of 1:5,000 ...
• Spontaneous mutations that confer resistance to 2-deoxyglucose act through Hxk2 and Snf1 pathways to regulate gene expression and HXT endocytosis

  Samantha R. Soncini, et al., bioRxiv - Genetics 2019

  Quote: ... Proteins tagged with the HA epitope (Snf1, Glc7) were detected with Anti-HA probe (Santa Cruz) diluted 1:2,000 ...
• The Verticillium longisporum phospholipase VlsPLA₂ is a virulence factor targets host nuclei and modulates plant immunity

  Vahideh Rafiei, et al., bioRxiv - Microbiology 2023

  Quote: ... GFP-tagged protein was detected using the B2 anti-GFP HRP-conjugated antibody (Santa Cruz Biotechnology), and HA-tagged proteases were detected using the anti-HA peroxidase-conjugated antibody (Sigma) ...
• BRCA1-BARD1 regulates transcription through modulating topoisomerase IIβ

  Jaehyeon Jeong, et al., bioRxiv - Molecular Biology 2020

  Quote: ... The reaction was stopped by the addition of 8X SDS loading buffer and unmodified and ubiquitinated recombinant TOP2B was detected using His antibody (sc-8036, Santa Cruz biotechnology, USA). For E2 ubiquitin-conjugating enzyme screening ...
• The PELOTA-HBS1 Complex Orchestrates mRNA Translation Surveillance and PDK1-mediated Plant Growth and Development

  Wei Kong, et al., bioRxiv - Plant Biology 2021

  Quote: ... GST-tagged proteins were detected by a mouse anti-GST antibody (sc-138, 1:3000, Santa Cruz Biotechnology). Actin was detected by a mouse anti-actin antibody (M20009 ...
• Two paralogous PHD finger proteins participate in Paramecium tetraurelia’s natural genome editing

  Lilia Häußermann, Aditi Singh, Estienne C. Swart, bioRxiv - Molecular Biology 2024

  Quote: ... HA-tagged proteins were detected with an HRP-conjugated anti-HA antibody (sc-7392 HRP, Santa Cruz, USA) diluted 1:500 in PBST and incubated overnight at 4°C ...
• Chloroplast chaperonin-mediated targeting of a thylakoid membrane protein

  Laura Klasek, Kentaro Inoue, Steven M. Theg, bioRxiv - Plant Biology 2020

  Quote: ... anti-His (Santa Cruz), anti-OE33 (Chou et al. ...
• CNS myelination requires VAMP2/3-mediated membrane expansion in oligodendrocytes

  Mable Lam, et al., bioRxiv - Neuroscience 2022

  Quote: ... to visualize biotinylated proteins and anti-GFP conjugated to Alexa Fluor 488 (1:1000, Santa Cruz sc-9996 AF488) in 3% BSA 1xPBST overnight at 4°C ...
• Engineered high-density lipoprotein particles that chaperone bioactive lipid mediators to combat endothelial dysfunction and thromboinflammation

  Steven Swendeman, et al., bioRxiv - Biochemistry 2022

  Quote: ... and His-tag (Santa Cruz) expression (data not shown) ...
• TRIM 25 nuclear translocation is hampered by localization to influenza A virus non-structural protein 1 cytosolic aggregates

  Anne Weiß, Ivan Haralampiev, Susann Kummer, bioRxiv - Microbiology 2022

  Quote: ... and probed with first antibodies (rabbit anti-phospho-IRF3, catalogue no.4947, Cell Signalling, mouse anti-NS1, catalogue no. sc-130568, Santa Cruz, rabbit anticalnexin ...
• The HIV-1 Vpu transmembrane domain topology and formation of a hydrophobic interface with BST-2 are critical for Vpu-mediated BST-2 downregulation

  Nabab Khan, et al., bioRxiv - Cell Biology 2020

  Quote: ... for EGFP-tagged Vpu/F1/F2 detection, Nef antibody (EEH1, NIH AIDS Reagent Program) and anti-HA antibody for HA-tagged BST-2 protein (Santa Cruz). Secondary antibodies conjugated to HRP (Calbiochem ...
• Identification of plant exclusive lipid transfer SMP proteins at membrane contact sites in Arabidopsis and Tomato

  Carolina Huercano, et al., bioRxiv - Plant Biology 2022

  Quote: ... The following primary antibodies were used for detection of epitope-tagged proteins: mouse monoclonal anti-GFP clone B-2 (1:1,000, catalog no. sc-9996, Santa Cruz Biotechnology) and anti-RFP antibody (1/2000 ...
• FUS binding to RNA prevents R-loops

  Valery F. Thompson, et al., bioRxiv - Biochemistry 2023

  Quote: Recombinant expressed FUS protein (1.3 µg) and anti-FUS (4H11) antibody (11 µg; Santa Cruz Biotechnology, sc-47711) were immobilized by incubating together for 1 hour at room temperature in phosphate buffered saline (PBS ...
• Hypoxia Dynamically Regulates DBC1 Ubiquitination and Stability by SIAH2 and OTUD5 in Breast Cancer Progression

  Qiangqiang Liu, et al., bioRxiv - Cell Biology 2022

  Quote: ... His (1:1000, Santa Cruz Biotech.), GST (1:1000 ...
• Discovery of a small molecule that selectively destabilizes Cryptochrome 1 and enhances life span in p53 knockout mice

  Seref Gul, et al., bioRxiv - Molecular Biology 2021

  Quote: ... anti-His (Santa Cruz SC-8036) BetaActin (Cell Signaling ...
• A Composite Biomarker Signature of Type 1 Diabetes Risk Identified via Augmentation of Parallel Multi-Omics Data from a Small Cohort

  Oscar Alcazar, et al., bioRxiv - Biochemistry 2024

  Quote: ... incubated with horse-radish peroxidase-linked recombinant mouse IgG Fc binding protein (#525409; Santa Cruz Biotech, Dallas, TX, USA) for 1 hour at room temperature ...
• Local PI(4,5)P₂ pool dynamics detected by the coincidence biosensor tubbyCT maintain the integrity of ER-PM junctions during PLC signaling

  Veronika Thallmair, et al., bioRxiv - Cell Biology 2020

  Quote: Myc- and GFP-tagged proteins were detected by rabbit polyclonal IgG anti-GFP (FL) (1:200; sc-8334 Santa Cruz Biotechnology, Dallas, US) and mouse monoclonal IgG anti-c-Myc (1:200 ...
• Synaptotagmins Maintain Diacylglycerol Homeostasis at Endoplasmic Reticulum-Plasma Membrane Contact Sites during Abiotic Stress

  Noemi Ruiz-Lopez, et al., bioRxiv - Molecular Biology 2020

  Quote: ... The following primary antibodies were used for detection of epitope-tagged proteins: mouse monoclonal anti-GFP clone B-2 (1:1000, catalog no. sc-9996, Santa Cruz Bio-technology) and rabbit polyclonal anti-SYT1 antibody (1:5000) ...
• Significant roles in RNA-binding for the amino-terminal domains of Drosophila Pumilio and Nanos

  Tammy H. Wharton, et al., bioRxiv - Molecular Biology 2023

  Quote: ... The epitope-tagged Pum and α-tubulin proteins were detected by successive incubation with anti-Myc 9E10 monoclonal primary (SC-40, Santa Cruz Biotechnology) and anti-α-tubulin B-5-1-2 monoclonal primary (T5168 ...
• JMJD6 Cleaves MePCE to Release P-TEFb

  Schuyler Lee, et al., bioRxiv - Biochemistry 2019

  Quote: ... Anti-His (Santa Cruz Biotechnology, sc-533073), Anti-Pol II CTD (Gift from Dr ...
• A mechanism for how Cdr1/Nim1 kinase promotes mitotic entry by inhibiting Wee1

  Hannah E. Opalko, et al., bioRxiv - Cell Biology 2019

  Quote: ... anti-His (Santa Cruz Biotechnologies sc-8036), and anti-thiophosphate ester (Abcam ab92570).
• Inactivation of p21-Activated Kinase 2 (Pak2) Inhibits the Development of Nf2-Deficient Malignant Mesothelioma

  Eleonora Sementino, et al., bioRxiv - Cancer Biology 2020

  Quote: ... Then virus particles and polybrene (sc-134220; Santa Cruz) at a concentration of 2 μg/ml were added to the plate ...
• A mechanism of uncompetitive inhibition of the serotonin transporter

  Shreyas Bhat, et al., bioRxiv - Pharmacology and Toxicology 2022

  Quote: ... These cells were transfected by combining either YFP-tagged WT-SERT or YFP-tagged SERT-PG601,602AA with PEI (linear 25 kDa polyethylenimine; Santa Cruz, SC-360988A) at a ratio of 1:3 (w/w ...
• Antitumor activity of a lectibody targeting cancer-associated high-mannose glycans

  Young Jun Oh, et al., bioRxiv - Cancer Biology 2021

  Quote: ... HRP tagged secondary antibodies (Anti-rabbit IgG, Santa Cruz, Dallas ...
• PDR6-mediated camalexin efflux and disease resistance are regulated through direct phosphorylation by the kinases OXI1 and AGC2-2

  Guopeng Miao, et al., bioRxiv - Plant Biology 2022

  Quote: ... and anti-HIS antibodies (Santa Cruz Biotechnology, USA).
• SERBP1 interacts with PARP1 and is present in PARylation-dependent protein complexes regulating splicing, cell division, and ribosome biogenesis

  Kira Breunig, et al., bioRxiv - Genomics 2024

  Quote: ... anti-His (Santa Cruz Biotechnology, Cat# sc-53073), anti-Flag (Invitrogen ...
• An evolutionarily conserved Lhx2-Ldb1 interaction regulates the acquisition of hippocampal cell fate and regional identity

  Veena Kinare, et al., bioRxiv - Neuroscience 2020

  Quote: ... and biotinylated anti-Ldb1 antibody (Santa Cruz, SC 11198).
• Evolution and antiviral activity of a human protein of retroviral origin

  John A. Frank, et al., bioRxiv - Molecular Biology 2022

  Quote: ... Transfected cells were infected with reporter virus by applying virus (HIV-RD114env, HIV-VSVg, HIV-SMRVenv) stocks in the presence of polybrene (Santa Cruz Bio, sc-134220) at a final concentration of 4 ug/mL ...
• A Real-Time Analysis of Protein Transport via the Twin Arginine Translocation Pathway in Response to Different Components of the Protonmotive Force in vivo

  Wenjie Zhou, et al., bioRxiv - Biochemistry 2023

  Quote: ... monoclonal antibody was used to probe for FLAG-tagged SufI or FLAG-tagged SufI-pep86 followed by a horseradish peroxidase-conjugated α-mouse antibody (Santa Cruz Biotechnology Inc.). Labeled proteins were visualized using either ProSignal Pico or ProSignal Femto ECL Western Blotting detection kit (Prometheus).
• Plant Cleavage Factor I complex is essential for precise cleavage and polyadenylation site determination

  Lukasz Szewc, et al., bioRxiv - Molecular Biology 2024

  Quote: ... the HIS-tagged AtCFI25a protein bound to GST-AtCFI59 was detected by western blot analysis using an anti-HIS antibody (G18) (sc-804; Santa Cruz Biotechnology, Dallas, USA) at 1:5000 dilution.
• Macrophage Receptor with collagenous structure (MARCO) recognizes the cellular protein corona formed on environmental particles

  Seishiro Hirano, Sanae Kanno, bioRxiv - Cell Biology 2023

  Quote: ... HRP-tagged goat anti-mouse IgG (sc-2354, Santa Cruz), and HRP-tagged goat anti-rabbit (Cell Signaling ...
• Tumor protein D52 (TPD52) affects cancer cell metabolism by negatively regulating AMPK

  Yali Chen, et al., bioRxiv - Cell Biology 2021

  Quote: ... Anti-His tag (sc8036, WB: 1:1000; Santa Cruz), Rabbit anti-FLAG (F7425 ...
• Chromatin-associated microprocessor assembly is regulated by PRP40, the U1 snRNP auxiliary protein

  Agata Stepien, et al., bioRxiv - Molecular Biology 2022

  Quote: ... anti-His (sc-8036, Santa Cruz Biotechnology; 1: 1000), anti-rabbit (AS09 602 ...
• DEPDC1/ EEF1A1 complex promotes the progression of human osteosarcoma via downregulation of FOXO3a

  Lin Shen, et al., bioRxiv - Cell Biology 2021

  Quote: ... or anti-HIS-antibody (1:300; Santa Cruz Biotechnology) for immunoprecipitation.
• Vimentin cysteine 328 modifications finely tune network organization and influence actin remodeling under oxidative and electrophilic stress

  Patricia González-Jiménez, et al., bioRxiv - Cell Biology 2023

  Quote: ... (+)-Biotin-(PEO)3-iodoacetamide (biotinylated iodoacetamide) was from Santa Cruz Biotechnology ...
• A SARS-CoV-2 neutralizing antibody protects from lung pathology in a COVID-19 hamster model

  Jakob Kreye, et al., bioRxiv - Immunology 2020

  Quote: Recombinant SARS-CoV-2-S1 protein produced in HEK cells (Creative Diagnostics, DAGC091) was covalently labeled using CruzFluor647 (Santa Cruz Biotechnology, sc-362620) according to the manufacturer’s instructions.
• Study of an FBXO7 patient mutation reveals Fbxo7 and PI31 co-regulate proteasomes and mitochondria

  Sara Al Rawi, et al., bioRxiv - Neuroscience 2021

  Quote: ... 20 μM human recombinant ubiquitin (Santa Cruz) and 2 mM ATP (Bio-Techne ...
• Fbxo7 promotes Cdk6 activity to inhibit PFKP and glycolysis in T cells

  Rebecca Harris, et al., bioRxiv - Cell Biology 2021

  Quote: ... 20 µM human recombinant ubiquitin (Santa Cruz) and 2 mM ATP (Bio-Techne ...
• Late stages of the Zika virus life cycle are impaired by a selective TRPML2 agonist

  Kerstin K. Schwickert, et al., bioRxiv - Cell Biology 2024

  Quote: ... raised against aa112-608 of recombinant pORF2 protein as used in a previous study (Glitscher et al., 2021a)) as well as anti-GAPDH (Santa Cruz Biotechnology, 1:2,000) and anti-beta-Actin (Sigma-Aldrich ...