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Citations for Santa Cruz :
1 - 50 of 740 citations for Nuclear Protein Extraction since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: Protein extraction or immunoprecipitation (IP) using Protein A/G PLUS-Agarose (Santa Cruz biotechnology; sc-2003) (58) ...
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bioRxiv - Immunology 2024Quote: ... 10% of nuclear extract was saved as input and rest incubated with protein A/G agarose (Santa Cruz) for 30 min at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... Fractionation was verified by immunoblotting using Abs against the nuclear proteins lamin A/C (Santa Cruz Biotechnology, sc-20681) at a dilution of 1:1,000 and the cytoplasmic protein GAPDH (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... the nuclear stain DAPI (Santa Cruz Biotechnology) and F-actin stain Phalloidin-Atto 633 (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... the nuclear stain DAPI (Santa Cruz Biotechnology) was added for 10 minutes ...
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bioRxiv - Physiology 2021Quote: ... Proliferation Cell Nuclear Antigen (PCNA) (Santa Cruz Biotechnology), Tamm-Horsfall protein (Santa Cruz Biotechnology) ...
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bioRxiv - Immunology 2022Quote: ... goat anti-proliferating cell nuclear antigen (PCNA; Santa Cruz Biotechnology ...
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bioRxiv - Cancer Biology 2022Quote: ... Hoechst 33342 nuclear stain (Santa Cruz Biotechnology #SC-495790) at 1:1000 dilution ...
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bioRxiv - Neuroscience 2023Quote: ... using DAPI for nuclear labeling and anti-NFAT (NFATc3, Santa Cruz Biotechnology). Cover slides were mounted and observed on a Fluoview FV10i (Olympus Co. ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... or Proliferating Cell Nuclear Antigen (PCNA, Santa Cruz, CA, USA, 1:200 dilution) and IL6 (Abcam ...
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bioRxiv - Biochemistry 2021Quote: ... anti-phosphorylated nuclear factor kappa-B (p-NFκB) (Santa Cruz Biotechnology, USA, sc-101751), anti-Bcl-2-associated X protein (Bax ...
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bioRxiv - Neuroscience 2021Quote: ... and nuclear compartments were incubated with antibodies for ERα (mouse monoclonal, Santa Cruz; 1:750). Samples from cytosolic fractions were incubated with antibodies for cytosolic loading control Enolase (1:2000 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... A Combination of antibodies against Proliferating Cell Nuclear Antigen (PCNA, Santa Cruz, CA, USA, 1:200 dilution) and histone H2A.XS139ph (phospho Ser139 ...
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bioRxiv - Molecular Biology 2024Quote: ... Nuclear DNA was stained with 4’,6-diamidino-2-phenylindole (DAPI) (Santa Cruz, catalog no. sc-3598) and the coverslips were mounted on a glass slide using Vectashield mounting medium (ThermoFisher) ...
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bioRxiv - Genomics 2019Quote: ... The nuclear lamina was visualized using an anti-LMNB antibody (sc-6217, goat IgG; Santa Cruz Biotechnology, Inc.) and Alexa Fluor 647 AffiniPure Donkey Anti-Goat (Jackson Immunoresearch ...
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bioRxiv - Cancer Biology 2019Quote: ... Nuclear extract (NE) was prepared from these cells and incubated with a polyclonal p53 antibody (FL393, Santa Cruz) prior to addition of protein G beads ...
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bioRxiv - Developmental Biology 2020Quote: ... and immersed in primary antibodies specific for proliferating cell nuclear antigen (PCNA; sc-56, 1:200, Santa Cruz Biotechnology) or human leukocyte antigen-G (HLAG ...
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bioRxiv - Pathology 2020Quote: ... anti-receptor activator of nuclear factor kappa-B ligand (anti-RANKL) (SC-7628, Santa Cruz Biotechnology, Santa Cruz, CA, USA) and anti-osteoprotegerin (anti-OPG ...
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bioRxiv - Pathology 2020Quote: ... anti-receptor activator of nuclear factor kappa-B ligand (anti-RANKL) (SC-7628, Santa Cruz Biotechnology, Santa Cruz, CA, USA) and anti-osteoprotegerin (anti-OPG ...
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bioRxiv - Developmental Biology 2020Quote: ... Primary antibodies against proliferating cell nuclear antigen (PCNA-1, 1/200, sc-7907, Santa Cruz; PCNA-2, 1/200, M087901, Dako) were applied overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... and harvested for either whole cell lysates or nuclear extracts before subjecting to SDS-PAGE and Western Blotting with a human anti-SOX18 antibody (sc166025 from Santa Cruz), anti-HaloTag antibody (G9281 from Promega) ...
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bioRxiv - Neuroscience 2023Quote: ... 3mg of nuclear lysates were used for IP with 2 μg IgG or 5 μg primary antibodies (normal mouse IgG, Santa Cruz Biotechnology ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies used for this study were as follows: mouse anti-proliferating cell nuclear antigen (PCNA; Santa Cruz Biotechnology, sc-25290), 1:500 ...
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bioRxiv - Cell Biology 2022Quote: ... The following primary antibodies were used: mouse monoclonal antibody against Proliferation Cell Nuclear Antigen (PCNA, sc56 Santa Cruz, CA, USA, 1:50 dilution) and rabbit polyclonal against myeloperoxidase (MPX ...
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bioRxiv - Bioengineering 2023Quote: ... Blood was shipped on dry ice immediately following extraction to Dovetail Genomics (Santa Cruz) for de novo genome assembly.
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bioRxiv - Cell Biology 2019Quote: Nuclear translocation of p65 NFκB was studied with Image J by measuring colocalisation of nuclear stain DAPI with p65NFκB stained with rabbit p65 antibody (1:1000, Santa Cruz Biotechnology, SC-372-G) and TRITC-labelled goat anti-rabbit antibody (1:200 ...
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bioRxiv - Cell Biology 2022Quote: ... anti-nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor α (IκBα) (#J1512, Santa Cruz Biotechnology, Dallas, TX, USA, 1:500), anti-phospho-signal transducer and activator of transcription 1 (P-STAT1 ...
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bioRxiv - Immunology 2020Quote: ... Membranes were probed with primary anti-human nuclear factorkappa B (NF-κB) p65 (c-20) sc-372 (1:200 dilution, Santa Cruz Biotechnology, Palo Alto, CA, USA) overnight at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... 40μg of protein lysates will be incubated with anti-HDAC1 protein (from Santa Cruz, USA) followed by protein G-PLUS agarose precipitation ...
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bioRxiv - Biochemistry 2023Quote: ... Ab-protein-DNA complexes were captured by protein A-G agarose beads (Santa Cruz Biotechnology) for 4 h at 4 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... The cell lysates were pre-cleared with protein A or protein G beads (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2020Quote: ... protein lysates were pre-cleared with 50μl protein A/G plus agarose beads (Santa Cruz Biotechnology) by rocking at 4°C for an hour ...
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bioRxiv - Physiology 2019Quote: ... 15 μl of 50% (w/v) protein A/G or protein A beads (Santa Cruz Biotechnology) were added to the supernatant and rotated for 30 min to preclear the samples ...
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bioRxiv - Pathology 2019Quote: ... Cell lysates (500μg total protein) were incubated with 20μl Protein A/G PLUS-Agarose (Santa Cruz) by occasional gentle mixing for 6h at 4°C ...
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bioRxiv - Pathology 2019Quote: ... Cell lysates (300μg total protein) were incubated with 20μl Protein A/G PLUS-Agarose (Santa Cruz) by occasional gentle mixing for 12h at 4°C to remove the non-specific binding proteins ...
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bioRxiv - Cancer Biology 2020Quote: ... CREB binding protein (CBP; Santa Cruz), cyclin A (Novocastra) ...
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bioRxiv - Neuroscience 2020Quote: Protein G PLUS-Agarose (Santa Cruz Biotechnology #sc-2002 ...
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bioRxiv - Physiology 2021Quote: ... Tamm-Horsfall protein (Santa Cruz Biotechnology); Parvalbumin (ThermoScientific ...
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bioRxiv - Biochemistry 2023Quote: ... Protein A/G beads (Santa Cruz) were added to the lysates for 1 hr at 4 °C to capture the precipitate ...
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bioRxiv - Cell Biology 2021Quote: ... Antibody-associated protein complexes were isolated by 2 h incubation with protein A-agarose (Santa Cruz Biotechnology) at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Recombinant human IgG1-tagged proteins were then purified by the Protein A/G agarose (Santa Cruz Biotechnology). Proteins were eluted with 100 mM glycine pH 3.0 and collected in tubes containing 1 M Tris buffer ...
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bioRxiv - Plant Biology 2021Quote: ... Specific protein levels were assayed by Western blot protein using primary antibodies against GFP (Santa Cruz SC-9996) along with an HRP-conjugated goat anti-mouse IgG secondary antibody (Sigma Aldrich A4416).Total protein was visualized after transfer using Ponceau S red staining ...
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bioRxiv - Cell Biology 2020Quote: ... 10 μl Dynabeads protein G (Santa Cruz) were added to the lysate for 1 h at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... coupled with Protein A slurry (Santa Cruz). Non-specific antiserum (Santa Cruz ...
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bioRxiv - Neuroscience 2020Quote: ... protein A/G agarose beads (Santa Cruz) were washed in immunoprecipitation buffer (1% NP-40 ...
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bioRxiv - Physiology 2021Quote: ... Protein A/G-agarose beads (Santa Cruz). Saponin (Calbiochem) ...
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bioRxiv - Cancer Biology 2022Quote: ... 9610-5-D)/protein-A agarose (Santa Cruz sc-2003) and incubated with H4K16Ac antibody (Millipore 07-329)-protein A agarose overnight at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... then protein A/G agarose (Santa Cruz) was added and incubated for 3h at 4□ ...
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bioRxiv - Biochemistry 2019Quote: ... Protein G agarose beads (Santa Cruz Biotechnology) or FLAG beads (Sigma ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein A/G Plus beads (Santa Cruz) were added at a dilution of 1:10 and then incubated overnight at 4 °C on rotation ...