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1 - 50 of 521 citations for Estradiol Serum ELISA Kit 5 Plate since 2019

• The PAR2 signal peptide prevents premature receptor cleavage and activation

  Belinda Liu, et al., bioRxiv - Biochemistry 2019

  Quote: ... 50 ul of the conditioned medium from each transfection was incubated in one well of a 96-well ELISA plate (UltraCruz® ELISA Plate, high binding, 96 well, Flat bottom, Santa Cruz Biotechnology) at 37°C for 1 hr to allow protein in the media to adsorb to the plates ...
• Post-exposure intranasal IFNα suppresses replication and neuroinvasion of Venezuelan Equine Encephalitis virus within olfactory sensory neurons

  Matthew D. Cain, et al., bioRxiv - Immunology 2023

  Quote: ... 5% normal donkey serum (Santa Cruz Biotechnology) with 0.1% Triton X-100 (Sigma-Aldrich) ...
• Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccine

  Alexandra Atalis, et al., bioRxiv - Immunology 2022

  Quote: Neutralizing antibodies were quantified like the ELISA method described above in a 384-well UltraCruz® ELISA high-binding plate (Santa Cruz Biotechnology). Diluted spike in 0.05 M carbonate-bicarbonate buffer (1 μg spike/mL ...
• SRSF2 is a key player in orchestrating the directional migration and differentiation of MyoD progenitors during skeletal muscle development

  Rula Sha, et al., bioRxiv - Developmental Biology 2024

  Quote: ... The sections were then blocked with 5% normal goat serum (Santa Cruz Biotechnology) for 1hour and incubated with primary antibodies overnight at 4℃ ...
• Genetic ablation of the mitochondrial ribosome in Plasmodium falciparum sensitizes the human malaria parasite to antimalarial drugs targeting mitochondrial functions

  Liqin Ling, et al., bioRxiv - Biochemistry 2020

  Quote: ... Ultrathin sections of 50 nm were blocked with 5% FBS (fetal bovine serum)/5% NGS (normal goat serum) for 30 min and subsequently incubated with mouse anti-HA (sc-7392, Santa Cruz Biotechnology) for 1 h at room temperature ...
• Membrane-bound Interleukin-1α mediates leukocyte adhesion during atherogenesis

  Christina Mäder, et al., bioRxiv - Immunology 2023

  Quote: ... and washed and incubated with the primary antibodies were either diluted in 5% NFDM or 5% bovine serum albumin (BSA, Serva, USA) overnight at 4°C: hIL-1α (1:500, Santa Cruz, USA), mIL-1α (1:1,000 ...
• The heme exporter FLVCR regulates mitochondrial biogenesis and redox balance in the hematophagous insect Rhodnius prolixus

  Ana Beatriz Walter-Nuno, et al., bioRxiv - Biochemistry 2023

  Quote: ... diluted in Leibovitz-15 medium containing 5% fetal bovine serum or with DHE and 5 µM MitoTEMPO (Santa Cruz, Dallas, TX, United States). The midguts were incubated in the dark at 28 °C for 20 min ...
• Type I Interferon Signaling on Antigen-Presenting Cells Blunts Cell-Mediated Immunity Toward Listeria monocytogenes

  Zachary T. Morrow, John-Demian Sauer, bioRxiv - Immunology 2022

  Quote: ... Half-volume ELISA plates (Fisher, #07-200-37) were coated overnight with 100 µL rat-α-mouse IFN-β antibody (Santa Cruz, # sc-57201) at 0.1 µg/mL in plate coating buffer (0.1 M carbonate [Thermo ...
• TRPV1 feed-forward sensitisation depends on COX2 upregulation in primary sensory neurons

  Tianci Li, et al., bioRxiv - Neuroscience 2020

  Quote: ... blocked in 5% normal goat serum and incubated in anti-COX-2 M19 (sc-1747, Santa Cruz) antibody (1:200 ...
• Enzymatic degradation of RNA causes widespread protein aggregation in cell and tissue lysates

  Johan Aarum, et al., bioRxiv - Molecular Biology 2019

  Quote: ... 5 μl of renatured proteins were mixed in a white 96-well plate (Santa Cruz Biotechnology) with ATP (100 uM final concentration ...
• Activation of STING due to COPI-deficiency

  Annemarie Steiner, et al., bioRxiv - Immunology 2020

  Quote: ... Membranes were probed with primary antibody diluted in 5% skim milk/TBST or 5% Bovine Serum Albumin (BSA)/TBST over night at 4°C: anti-COPA (Santa Cruz Biotechnology, clone H-3, sc-398099), anti-phospho-STAT1 Tyr701 (Cell Signaling Technology ...
• Oncoprotein GT198 is a direct target of taxol

  Zheqiong Yang, et al., bioRxiv - Pharmacology and Toxicology 2019

  Quote: ... 5 μl anti-GT198 rabbit serum were captured by 10 μl Protein A/G agarose (Santa Cruz, sc-2003), washed ...
• Neural stem cells traffic functional mitochondria via extracellular vesicles to correct mitochondrial dysfunction in target cells

  Luca Peruzzotti-Jametti, et al., bioRxiv - Neuroscience 2020

  Quote: ... blocked in 5% goat serum and stained with an anti-TOMM20 antibody (rabbit, Santa Cruz, Dallas, USA, 1:300). Representative images were acquired as described above for the integration analysis ...
• Physiological Levels of Estrogen and Progesterone Modulate CR3 expression on, and Neisseria gonorrhoeae Infection of, Primary, Human, Cervical Epithelial Cells

  Trevor J. Edwards, Jennifer L. Edwards, bioRxiv - Microbiology 2022

  Quote: ... Chemiluminescent detection of hormones bound to gonococcal constituents was achieved using anti-estradiol (ESTR-1) or –progesterone (XM207) monoclonal antibodies (both from Santa Cruz Biotechnology; Santa Cruz, CA) and the appropriate peroxidase-conjugated secondary antibodies.
• Arsenic trioxide resistance in acute promyelocytic leukemia: More to it than PML mutations

  Nithya Balasundaram, et al., bioRxiv - Cancer Biology 2020

  Quote: ... followed by blocking using 5% goat serum and incubated with primary antibody (PML PG-M3 -Santa Cruz Biotechnologies, CA, USA) overnight at 4°C ...
• Invasive dedifferentiated melanoma cells inhibit JAK1-STAT3-driven actomyosin contractility of human fibroblastic reticular cells of the lymph node

  Christopher Rovera, et al., bioRxiv - Cancer Biology 2021

  Quote: ... 5% Goat Serum for 1h and stained overnight at 4°C with antibodies targeting YAP (#sc-271134, Santa Cruz Biotechnology) and P-MLC2 (S19 ...
• An Enhancer Trap system to track developmental dynamics in Marchantia polymorpha

  Alan O. Marron, et al., bioRxiv - Plant Biology 2023

  Quote: ... Auxin synthesis inhibitor treatments were done by growing gemmae on plates made with 10µM 5-(4-chlorophenyl)-4H-1,2,4-triazole-3-thiol (yucasin) (ChemCruz, Santa Cruz Biotechnology) or 100µM L-kynurenine (Sigma ...
• ACAT1/SOAT1 Blockade Suppresses LPS-Mediated Neuroinflammation by Modulating the Fate of Toll-Like Receptor 4 in Microglia

  Haibo Li, et al., bioRxiv - Neuroscience 2022

  Quote: ... Cells were incubated in RPMI (with 5% goat serum) with 1:200 dilution of specific anti-TLR4 (Santa Cruz sc-293072) antibodies at 4°C for 1 hr then washed twice in cold RPMI to remove unbound antibodies ...
• Physiological Levels of Estrogen and Progesterone Modulate CR3 expression on, and Neisseria gonorrhoeae Infection of, Primary, Human, Cervical Epithelial Cells

  Trevor J. Edwards, Jennifer L. Edwards, bioRxiv - Microbiology 2022

  Quote: ... Chemiluminescent detection of hormones bound to gonococcal constituents was achieved using anti-estradiol (ESTR-1) or –progesterone (XM207) monoclonal antibodies (both from Santa Cruz Biotechnology; Santa Cruz, CA) and the appropriate peroxidase-conjugated secondary antibodies.
• Novel fragile X syndrome 2D and 3D brain models based on human isogenic FMRP-KO iPSCs

  Carlo Brighi, et al., bioRxiv - Neuroscience 2020

  Quote: ... Tissues were then incubated overnight with primary antibodies in 0.3% Triton-X and 5% goat serum in PBS at the following dilutions: mouse anti-PAX6 (sc81649 Santa Cruz Biotechnology, 1:50), rabbit anti-FOXG1 (ab18259 Abcam ...
• Zmiz1 is required for mature β-cell function and mass expansion upon high fat feeding

  Tamadher A. Alghamdi, et al., bioRxiv - Physiology 2022

  Quote: ... then probed with primary antibodies in 5% bovine serum albumin (BSA) in TBST overnight at 4°C with the following concentrations: Zmiz1(1:1000, Santa Cruz sc-367825), CD81 (dilution 1:1000 ...
• Snail maintains the stem/progenitor state of skin epithelial cells and carcinomas through the autocrine effect of the matricellular protein Mindin

  Krithika Badarinath, et al., bioRxiv - Cell Biology 2021

  Quote: ... the membrane was blocked using either 5% BSA (Bovine Serum Albumin) in Tris buffer Saline containing 0.1% Tween (TBST) for phosphorylated proteins or 5% Blotto (Santa Cruz Biotechnology, sc-2325) for 60 min ...
• BACE1 – but not BACE2 – function is critical for metabolic disorders induced by high-fat diets in C57BL/6N mice

  Thomas W. Rosahl, et al., bioRxiv - Physiology 2021

  Quote: ... Deparaffinized sections were blocked in 5% normal goat serum followed by overnight incubation with primary antibodies at 4°C: anti-BACE2 antibody (Santa Cruz; 1:1000), anti-insulin (Invitrogen ...
• The GEF-GAP pair Solo and DLC3 balances Rho activity during endocytic transport

  Cristiana Lungu, et al., bioRxiv - Cell Biology 2022

  Quote: ... blocked in 5% (v/v) goat serum in PBS and incubated with an antibody recognising the extracellular domain of EGFR (Santa Cruz, Cat# sc-101). This was followed by permeabilization as described above and incubation with an antibody recognising the C-terminus of EGFR (Santa Cruz ...
• Large scale interrogation of retinal cell functions by 1-photon light-sheet microscopy

  Suva Roy, et al., bioRxiv - Neuroscience 2022

  Quote: ... The retinas were fixed in 4% PFA for 45 minutes at room temperature and then incubated in 5% normal donkey serum (Jackson Immuno, C840D36) in 1X phosphate buffer saline (PBS) with azide (Santa Cruz Biotechnology, SC-296028) containing 0.5% Triton X-100 (Sigma Aldrich ...
• Epoxy fatty acid dysregulation and neuroinflammation in Alzheimer’s disease is resolved by a soluble epoxide hydrolase inhibitor

  Anamitra Ghosh, et al., bioRxiv - Neuroscience 2020

  Quote: ... or TBS with 5% bovine serum albumin (BSA) followed by antibody incubation: sEH (1:300, Santa Cruz or 1:1000, Dr. Bruce Hammock’s lab (26)) ...
• Recurring EPHB1 mutations in human cancers alter receptor signalling and compartmentalisation of colorectal cancer cells

  Snehangshu Kundu, et al., bioRxiv - Cancer Biology 2023

  Quote: ... The middle part of the membrane was blocked with 5% bovine serum albumin (BSA) in TBST followed by incubation with mouse monoclonal anti-β-Actin (Santa Cruz Biotechnology, USA; #47778, 1:10,000) primary antibody at RT for 1 h as loading control ...
• Neuroimmune characterization of optineurin insufficiency mouse model during ageing

  Nikolina Mohovic, et al., bioRxiv - Immunology 2023

  Quote: ... The capture antibody for IFN-β ELISA (#sc-57201) was from Santa Cruz (Dallas, TX, USA), the detection antibody (#32400-1 ...
• Inhibition of PLK1-dependent EBNA2 phosphorylation promotes lymphomagenesis in EBV-infected mice

  Xiang Zhang, et al., bioRxiv - Cancer Biology 2021

  Quote: ... and a p300 specific serum (C20; Santa Cruz). For immunoprecipitations and Western blotting the equivalent of 1×107 cells was lysed in 500 μl of lysis buffer (1% NP40 ...
• ALPPL2 is a highly specific and targetable tumor cell surface antigen

  Yang Su, et al., bioRxiv - Cancer Biology 2020

  Quote: ... further blocked with 2% donkey serum (Santa Cruz Biotechnology) and avidin/biotin (Vector Laboratories) ...
• Reduced glutamate decarboxylase 1 underlies morphine-promoted lung metastasis of triple-negative breast cancer in mice

  Shih-Hong Chen, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Bovine serum albumin (BSA) was purchased from Santa Cruz Biotechnology (SantaCruz ...
• Glucosylhydroxyceramides Modulate Secretion Machinery of a Subset of Plasmodesmata Proteins and a Change in the Callose Accumulation

  Arya Bagus Boedi Iswanto, et al., bioRxiv - Plant Biology 2020

  Quote: ... 5 µM FB1 (Santa Cruz), 50 µM PDMP (Santa Cruz) ...
• Mutant mice lacking alternatively spliced p53 isoforms unveil Ackr4 as a male-specific prognostic factor in Myc-driven B-cell lymphomas

  Anne Fajac, et al., bioRxiv - Cancer Biology 2023

  Quote: ... p21 (F-5, Santa Cruz) and actin (actin-HRP sc47778 ...
• Immunosuppressive role of PGE2 during human tuberculosis

  Joaquín Miguel Pellegrini, et al., bioRxiv - Immunology 2020

  Quote: ... and 1% bovine serum albumin (Santa Cruz Biotechnology, sc-2323A) for 15 min ...
• WNK1-dependent water influx is required for CD4⁺ T cell activation and T cell-dependent antibody responses

  Joshua Biggs O’May, et al., bioRxiv - Immunology 2023

  Quote: ... NP20-BSA (bovine serum albumin) (5μg/ml, Santa Cruz Biotechnology) was used to coat 96-well Maxisorp Immunoplates (Nalge Nunc International Corporation ...
• Identification of the potassium binding site in serotonin transporter SERT

  Eva Hellsberg, et al., bioRxiv - Biochemistry 2023

  Quote: ... in serum-free DMEM using polyethyleneimine 25kDa (PEI) (Santa Cruz Biotechnology ...
• Clinical and In Situ Characterization of Hepatitis Delta Virus in Sjogren’s Syndrome

  Matthew C. Hesterman, et al., bioRxiv - Microbiology 2022

  Quote: ... mouse monoclonal anti-Aquaporin 5 (APQ-5) antibody (1:400; Santa Cruz Biotechnology Inc, SC-514022), mouse monoclonal anti-Cytokeratin 7 (CK-7 ...
• Essential role of Bone morphogenetic protein 15 in porcine ovarian and follicular development and ovulation

  Yufeng Qin, et al., bioRxiv - Developmental Biology 2019

  Quote: ... Smad1/5/8 (Santa cruz, sc6031R), and p-Smad2/3 (Santa cruz ...
• Identification of proximal SUMO-dependent interactors using SUMO-ID

  Orhi Barroso-Gomila, et al., bioRxiv - Biochemistry 2021

  Quote: ... 5 μg/ml blasticidin (Santa Cruz) for U2OS ...
• A transcriptional mechanism involving R-loop, m6A modification and RNA abasic sites regulates an enhancer RNA of APOE

  Jason A. Watts, et al., bioRxiv - Molecular Biology 2022

  Quote: ... 5 μg mouse IgG (Santa Cruz) NELFA (Santa Cruz) ...
• Gain-of-function genetic screens in human cells identify SLC transporters overcoming environmental nutrient restrictions

  Manuele Rebsamen, et al., bioRxiv - Cell Biology 2022

  Quote: ... SLC3A2 (Santa Cruz, 376815, E-5), tubulin (Abcam ...
• Shigella sonnei infection of zebrafish reveals that O-antigen mediates neutrophil tolerance and dysentery incidence

  Vincenzo Torraca, et al., bioRxiv - Microbiology 2019

  Quote: ... Necrostatin-5 (10 μM, Santa Cruz) [46] ...
• Native Elongation Transcript sequencing reveals temperature dependent dynamics of nascent RNAPII transcription in Arabidopsis

  Peter Kindgren, Maxim Ivanov, Sebastian Marquardt, bioRxiv - Genomics 2019

  Quote: ... 5 μM pladienolide B (Santa Cruz) or 5 μM Herboxidiene (Focus Biomolecules ...
• Tumor-responsive, multifunctional CAR-NK cells cooperate with impaired autophagy to infiltrate and target glioblastoma

  Jiao Wang, et al., bioRxiv - Immunology 2020

  Quote: ... (5) was purchased from Santa Cruz Biotechnology ...
• ETV7 reduces inflammatory responses in breast cancer cells by repressing TNFR1/NF-κB axis

  Erna Marija Meškytė, et al., bioRxiv - Cancer Biology 2022

  Quote: ... HSP70 (C92F3A-5, Santa Cruz Biotechnologies), TEL2 (E-1 ...
• The nucleolar aberrancies that drive ribosome impairment induced by RNA binding proteins are hallmarks of aging

  Pedro Aguilar-Garrido, et al., bioRxiv - Molecular Biology 2023

  Quote: ... p21/CIP1 (Santa Cruz, F-5), β-actin (Cell Signaling ...
• An unconventional mechanism of IL-1β secretion that requires Type I IFN in lupus monocytes

  Simone Caielli, et al., bioRxiv - Immunology 2023

  Quote: ... MG132 (5 mM; Santa Cruz Biotech) was added to the culture during phase 3 ...
• Sigma receptor ligands are potent anti-prion compounds that act independently of sigma receptor binding

  Robert C. C. Mercer, et al., bioRxiv - Neuroscience 2023

  Quote: ... σ1R (B-5, Santa Cruz Biotechnology); σ2R (26444-1-AP ...
• Addressing Heterogeneity in direct analysis of Extracellular Vesicles and analogues using Membrane-Sensing Peptides as Pan-Affinity Probes

  Alessandro Gori, et al., bioRxiv - Biochemistry 2023

  Quote: ... and anti human serum albumin (1:500, Santa Cruz, CA, USA). After washing with TBS-T ...
• An intranasally administrated SARS-CoV-2 beta variant subunit booster vaccine prevents beta variant viral replication in rhesus macaques

  Yongjun Sui, et al., bioRxiv - Immunology 2021

  Quote: ... using high-binding 96-well plates (Santa Cruz Biotechnology). After incubation at 4°C overnight ...