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Citations for Santa Cruz :
1001 - 1050 of 1116 citations for Recombinant Human CTLA4 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... pre-cleared with protein A agarose beads (Pierce) and incubated with agarose conjugated anti-Rad52 mAb (clone F-7; sc-365341 AC, Santa Cruz Biotechnology) at 4°C overnight ...
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bioRxiv - Neuroscience 2021Quote: ... samples containing same protein amount were subjected to immunoprecipitation with rabbit polyclonal antibody against PDE4B (H-56) (#sc-25812, Santa Cruz Biotechnology) coupled to GammaBind Plus Sepharose beads (# 17-0886-02 ...
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bioRxiv - Microbiology 2021Quote: ... with the lysate overnight at 4 °C followed by addition of Protein A/G PLUS-Agarose beads (Santa Cruz Biotechnology, Dallas, TX) and incubation for 2 hours at 4 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... Antibody shifts were done by incubating the protein/extract with 4uL of antibody (α-Twist: Invitrogen PA5-47824, α-Snail: Santa Cruz E-18 ...
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bioRxiv - Neuroscience 2020Quote: ... brains were incubated overnight with combinations of the following primary antibodies: rabbit anti-protein kinase C (anti-PKC) clone C20 (1:1000; Santa Cruz Biotechnology), rabbit anti-phospho-histone H3 (1:2500 ...
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bioRxiv - Microbiology 2019Quote: ... Primary antibody membranes were incubated with mouse IgG kappa binding protein conjugated to horseradish peroxidase (1:2500) (sc-516102, Santa Cruz Biotechnology) or goat anti-rabbit IgG conjugated to horseradish peroxidase (1:2500 ...
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bioRxiv - Microbiology 2019Quote: ... Membranes were incubated in TBST containing 2% (w/v) BSA with mouse IgG kappa binding protein conjugated to horseradish peroxidase (1:5000) (sc-516102, Santa Cruz Biotechnology) or goat anti-rabbit IgG conjugated to horseradish peroxidase (1:5000 ...
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bioRxiv - Cancer Biology 2020Quote: ... were cleared by incubating with 2.0 µg of the control IgG and 20 µL vortexed Protein G PLUS-Agarose beads (Santa Cruz, sc-2002) for 30 min with rocking at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... Thirty micrograms of total protein were separated by SDS-PAGE and probed with primary antibodies against NFATC2 (Santa Cruz Biotechnology, sc-7296), NFATC4 (Cell Signaling ...
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bioRxiv - Cell Biology 2021Quote: ... the total protein was detected by using specific antibodies (GRK2: Santa Cruz sc-13143; GRK3: Cell signaling technology #80362; GRK5: Santa Cruz, sc-518005 ...
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bioRxiv - Neuroscience 2022Quote: ... cell culture supernatants of cultured cerebellar neurons were incubated overnight with Reelin antibody G10 and Protein A/G beads (Santa Cruz Biotechnologies) or brain homogenates were resuspended in immunoprecipitation buffer (25mM Tris-HCl ...
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bioRxiv - Cell Biology 2022Quote: Cell lysates (500 μg) were pre-cleared with 30 μl of protein A/G plus-agarose beads (Santa Cruz, catalog #: sc-2003) and 1.0 μg of normal mouse IgG antibody (Santa Cruz ...
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bioRxiv - Cell Biology 2021Quote: Primary antibodies used for Western blot of β-actin and AK2 proteins were: anti-actin (Santa Cruz Biotechnology sc-47778, 1:1000), anti-AK2 (ProteinTech 11014-1-AP ...
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bioRxiv - Immunology 2019Quote: ... immunoprecipitated with 2 µg/sample of indicated antibodies overnight at 4 °C before addition of washed protein A/G PLUS-agarose beads (Santa Cruz Biotechnology) for 1 hr with rotation at 4 °C ...
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bioRxiv - Plant Biology 2021Quote: ... the protein-retained beads were boiled in Laemmli buffer and immunoblotted using anti-GST and anti-MBP antibodies (Santa Cruz Biotechnology, USA).
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bioRxiv - Neuroscience 2019Quote: ... or GT1-7MC4R cells (50 μg of protein) were subjected to immunoprecipitation overnight with 10 μl of a polyclonal ROCK1 antibody (sc-6055, Santa Cruz Biotechnology) coupled to protein G-Sepharose beads (GE healthcare ...
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bioRxiv - Plant Biology 2019Quote: ... Immunoprecipitated proteins were separated on SDS-PAGE acrylamide gels and western blots were performed using anti-GFP (Santa Cruz Biotechnology sc-9996), anti-SYT1 [7] ...
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bioRxiv - Plant Biology 2019Quote: ... transferred onto PVDF membrane and proteins were detected using an anti-c-myc monoclonal antibody (sc-40, Santa Cruz Biotech, Texas, USA) and anti-mouse HRP conjugate (W4021 ...
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bioRxiv - Cancer Biology 2021Quote: ... to detect DARPP-32 (Cat no.: sc-135877; Dilution 1:200) and α-Tubulin (Cat no.: sc-5286; Dilution 1:1000) protein were obtained from Santa Cruz Biotechnology ...
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bioRxiv - Cancer Biology 2021Quote: ... protein lysates (500 μL of NP-40 buffer containing 200 μg of protein) were incubated with 10 μg of primary antibody Neu (F-11) agarose conjugate (sc-7301; Santa Cruz Biotechnology) overnight at 4°C with gentle rotation ...
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bioRxiv - Bioengineering 2020Quote: Tissue sections were incubated overnight with polyclonal rabbit antibodies raised against c-fos protein (1:1000; Santa Cruz Biotechnology Inc.; sc-253), GFAP (1:1000 ...
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bioRxiv - Immunology 2020Quote: ... CBLB and GAPDH in the protein blots were probed with anti-CBLB- and anti-GAPDH-antibodies (SC-8006 and SC-166545, respectively; Santa Cruz Biotechnology). Flow Cytometry analysis ...
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bioRxiv - Cell Biology 2020Quote: ... Supernatants were pre-cleared with control IgG for 1 hr at 4 °C and Protein A/G Plus-Agarose beads (Santa Cruz Biotechnology) for 30 min at 4 °C before incubation with primary antibody overnight at 4 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... Cbl was immunoprecipitated from 20-50 mg of total protein from each treatment using anti-Cbl AB coupled to agarose beads (sc-170 AC, Santa Cruz Biotechnology). The immunoprecipitated proteins were eluted from Cbl AB-agarose beads with Pierce IgG Elution Buffer (21004 ...
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bioRxiv - Immunology 2022Quote: ... WCL were incubated with GLUT3 antibody overnight and then with Protein A/G PLUS-Agarose bead slurry (Santa Cruz Biotechnology, sc-2003) for 3h ...
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bioRxiv - Neuroscience 2023Quote: ... Immunodetection of ubiquitinated proteins was accomplished by incubating the membrane overnight with 1:1000 diluted monoclonal ubiquitin antibody P4D1 (sc-8017; Santa Cruz Biotechnology) and exposing the membranes to peroxidase-conjugated anti-rabbit secondary antibody and ECL Advance chemiluminescent substrate (GE Healthcare) ...
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bioRxiv - Molecular Biology 2022Quote: ... Reactions were incubated at 30 °C for 30 minutes or 1 hour before separation by SDS–PAGE followed by immunoblot analysis for MBP-substrate proteins (Anti-MBP-probe Antibody (R3.2) (Santa Cruz sc-53366)).
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bioRxiv - Immunology 2024Quote: ... Precleared extracts were incubated with 40 μL of 50% protein A/G agarose and 5 of normal rabbit IgG (Santa Cruz Biotech), H3K27ac (Active Motif ...
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bioRxiv - Immunology 2024Quote: ... Membranes were incubated in TBST containing 5% (w/v) skimmed milk with mouse IgG kappa binding protein (m-IgGκ BP) conjugated to HRP (1:2000) (sc-516102, Santa Cruz Biotechnology) for 3h at 4°C on a rotary and washed thrice with TBST for 10 min each time ...
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bioRxiv - Microbiology 2024Quote: ... the formation of a fusion protein with a proper size was validated by Western blotting using anti-myc antibody 9E10 (Santa Cruz Biotechnology), and anti-Xpress tag antibody R91025 (Invitrogen) ...
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bioRxiv - Immunology 2023Quote: ... 1 mg of hTCEpi cell lysate in cell lysis buffer was precleared with 25 μl of protein A/G plus agarose (SC-2003; Santa Cruz Biotechnology) for 2 hours at 4°C with rotation before the agarose was removed by centrifugation at 2,000 rcf for 10 min ...
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bioRxiv - Cancer Biology 2022Quote: ... samples were immunoprecipitated using monoclonal mouse anti-WWOX cocktail antibodies53 together with protein A/G plus agarose beads (Santa Cruz, Texas, USA) and incubated overnight at 4°C on a rotor ...
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bioRxiv - Genomics 2023Quote: ... Samples were pre-cleaned by incubating with 2–4 μg of normal immunoglobulin G (IgG) and 50 μl of A / G-agarose protein microspheres (Santa Cruz Biotechnology) for 1 hour at 4 °C with agitation for preclearance ...
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bioRxiv - Immunology 2023Quote: ... Membranes were washed and incubated with mouse IgG kappa-binding protein conjugated to horseradish peroxidase (m-IgGk-HRP, 1:4000) (Santa Cruz technologies) in 5% milk for 1 h at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Clarified lysates were incubated with the appropriate antibodies for 2h at 4°C and subsequently with protein A/G agarose beads (Santa Cruz Biotechnology) for 1h at 4°C ...
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bioRxiv - Bioengineering 2022Quote: The total proteins were extracted by lysing the cells with radioimmune precipitation assay (RIPA) buffer (Santa Cruz Biotechnology, Santa Cruz, CA, USA) that contained protease inhibitor for 30 min on ice followed by centrifuging at 4 C ...
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bioRxiv - Bioengineering 2022Quote: The total proteins were extracted by lysing the cells with radioimmune precipitation assay (RIPA) buffer (Santa Cruz Biotechnology, Santa Cruz, CA, USA) that contained protease inhibitor for 30 min on ice followed by centrifuging at 4 C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Aliquots of lysates containing 100 μg of protein were used for each immunoprecipitation reaction with indicated antibodies followed by adsorption to protein A/G PLUS-agarose beads (Cat#: sc-2003, Santa Cruz Biotechnology). DNA-protein cross-link was reversed by heating the samples to 65 °C overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... approximately 750 µg of solubilized proteins were incubated with 1 µg of anti-C/EBPβ mouse IgG (Santa Cruz Biotech, sc-7962) or normal mouse IgG (Santa Cruz Biotech ...
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bioRxiv - Microbiology 2024Quote: ... and the remaining three aliquots were immunoprecipitated overnight at 4 °C with the indicated antibody mixed with Protein A/G PLUS-Agarose (Santa Cruz Biotechnology). Then ...
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bioRxiv - Plant Biology 2024Quote: ... This diluted chromatin was then incubated overnight at 4°C with Protein A/G PLUS-Agarose beads (Santa Cruz Biotechnology; sc-2003) and antibodies ...
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bioRxiv - Cancer Biology 2021Quote: ... the extracted lysates were first pre-cleared with protein G-magnetic beads at 4 °C for 2 h followed by incubation with HOXB13 antibody (2 μg/sample, Santa Cruz, sc-28333) overnight ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein extracts were incubated with each antibody with rotation for 2 hr and then added to protein A/G agarose beads (Santa Cruz, sc-2003) for an additional 1 hr ...
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bioRxiv - Cell Biology 2020Quote: ... After centrifugation at 13,200 g for 15 min at 4°C, equal amounts of cell lysates were preincubated with mouse IgG (Applygene, C2118) and Protein A/G agarose beads (Santa Cruz, sc-2003) for 4 h at 4°C and then clarified by centrifugation ...
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bioRxiv - Cancer Biology 2020Quote: BCR-ABL protein expression was assessed by SDS-PAGE and western blotting using a mouse anti-ABL antibody (1:1,000, Santa Cruz Biotechnology, CA, USA). Horseradish peroxidase-conjugated α-mouse antibody (1:10,000 ...
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bioRxiv - Cancer Biology 2020Quote: ... A fraction of the mixture of protein-DNA complex was used as “input DNA.” Soluble chromatin was immunoprecipitated with anti-VDRE antibody (D-6, Santa Cruz Biotechnology Inc) and normal mouse immunoglobulin G (IgG ...
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bioRxiv - Molecular Biology 2022Quote: ... Clones were screened for gene deletion by PCR as well as LARP1 mRNA and protein depletion determined by qPCR and western blot analysis with a monoclonal LARP1 antibody (Santa Cruz sc-515873). Lesions at the locus of cutting were identified by PCR using genomic DNA to amplify the locus followed by Sanger sequencing and Synthego ICE analysis.
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bioRxiv - Developmental Biology 2022Quote: Western blotting was performed using 4–20% Mini-PROTEAN® TGX™ precast protein gels with the following antibodies anti-NFATc1 (1:1000, clone 7A6, Santa Cruz) and anti-GAPDH (1:2000 ...
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bioRxiv - Biochemistry 2021Quote: ... A precleaning step of the samples was performed by adding 50 μl of Protein G-Plus agarose beads suspension (Santa Cruz Biotechnology, INC) to 1 ml of each sample (Chimera ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were resolved by SDS-PAGE and identified by immunoblotting with a mouse anti-green fluorescent protein (GFP) (Santa Cruz Biotechnology, Santa Cruz, CA), or anti-Atg13 [175] antibody to detect tagged or endogenous proteins ...