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Citations for Miltenyi Biotec :
1 - 50 of 1614 citations for rno mir 143 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... The "Mouse Tregs detection kit" (Miltenyi Biotec®, Bergisch Gladbach, Germany) was used for regulatory T-cell staining ...
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bioRxiv - Molecular Biology 2019Quote: Apoptosis was analyzed using Annexin V-FITC apoptosis detection kit (Miltenyi Biotec). MDA-MB-231 and MCF-7 were incubated with recombinant IFNε for 48 h ...
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bioRxiv - Immunology 2019Quote: ... the cells were harvested and stained using the Treg detection kit (Miltenyi Biotec), resuspended in FACS buffer (1x PBS supplemented with 3% heat-inactivated and filtered FCS and 0.1%NaN3 ...
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bioRxiv - Immunology 2022Quote: ... we used human IFNγ secretion assay detection kit (PE) (Miltenyi Biotec, #130-054-202) as per the manufacturer’s protocol ...
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bioRxiv - Immunology 2019Quote: ... The staining procedure was executed as per the manufacturer instructions (Treg Detection Kit, Miltenyi Biotec). The cells were resuspended in FACS buffer (1x PBS supplemented with 3% heat-inactivated and filtered FCS and 0.1%NaN3 ...
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bioRxiv - Microbiology 2019Quote: ... Cells (106) were stained with the ‘Mouse IFN-γ secretion assay detection kit’ (Miltenyi Biotec, Germany) according to the manufacturer’s instructions and IFN-γ secretion was analyzed by flow cytometry.
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bioRxiv - Immunology 2023Quote: ... IFN-γ-secreting cells were detected using IFN-γ Secretion Assay/Detection Kit (PE) (Miltenyi Biotec) according to the manufacturer’s instructions and subsequently phenotypically characterised by flow cytometry.
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bioRxiv - Immunology 2020Quote: ... the cells were labeled with the IFN-γ Catch Reagent (IFN-γ Secretion Assay-Detection Kit, human Miltenyi Biotec) containing bispecific antibodies for CD45 and IFN-γ which was secreted by the stimulated target cells ...
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bioRxiv - Microbiology 2019Quote: ... or IFN-γ detection antibody (Miltenyi Biotec, Germany). After washing the cells ...
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bioRxiv - Cancer Biology 2020Quote: ... the population of IFNγ secreting cells was identified using the IFNγ Secretion assay detection kit (PE) (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... such T cells were selected using a human IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (Miltenyi Biotec, Auburn, CA) following the manufacturer’s instructions as previously described.38–42 Post-4 PBMC sample cryopreserved after monocyte depletion was thawed and cultured overnight in Yssel’s media (Gemini Bio Products ...
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bioRxiv - Immunology 2023Quote: ... purified CD8+ T-cells were coated with mouse IL-2 catch reagent from a mouse IL-2 secretion assay detection kit (Miltenyi Biotec) by incubating T cells in a 20x dilution of IL-2 catch reagent in R10 medium for 15 min on ice ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Myc for CAR detection (SH1-26e7.1.3, FITC, Miltenyi Biotec), CD14 (REA599 ...
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bioRxiv - Immunology 2021Quote: Tumor nodules collected from the lungs of KP mice at time of analysis were cut into 1mm pieces and placed in mouse tumor dissociation kit (Miltenyi Biotec) for 45 minutes at 37°C ...
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bioRxiv - Immunology 2020Quote: ... at RT and permeabilized with InsidePerm at 4°C (Miltenyi Biotec) followed by incubation with anti-IL-10-PE-conjugated antibody (Miltenyi Biotec) ...
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Inhibition of major histocompatibility complex-I antigen presentation by sarbecovirus ORF7a proteinsbioRxiv - Microbiology 2022Quote: ... PBMCs were stimulated for 3 hours with 10 μg/mL of Gag AK11 peptide and T cells producing IFN-γ in response were enriched using the IFN-γ Secretion Detection and Enrichment Kit (130-054-201; Miltenyi Biotec, Bergisch Gladbach, Germany) in accordance with the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Lineage Cell Detection Cocktail-Biotin (Miltenyi Biotec, #130-092-613, 1:100), Anti-Biotin MicroBeads (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: ... Lin–biotin (Lineage Cell Detection Cocktail-Biotin, Miltenyi Biotec, #130-092-613), Streptavidin–PE (BD Biosciences ...
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bioRxiv - Plant Biology 2019Quote: ... three times with wash buffer 1 (Miltenyi Biotec) and once with wash buffer 2 ...
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bioRxiv - Immunology 2020Quote: ... Fc receptors were blocked for 5 min at RT using FcR-blocking reagent (Miltenyi Biotec) and then incubated with fluorochrome-conjugated surface antibodies for 15 min at RT ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples at the 4 hour activation time point were also processed to isolate CD4+ and CD8+ T cells by positive CD4 and CD8 selection kits (Miltenyi Biotec, Cat# 130-045-101 and 130-045-201). Unstimulated T cells were processed at the 0 hour time point without any activation ...
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bioRxiv - Immunology 2020Quote: ... each sample was divided into two tubes for either cytokine detection with IFN-γ-FITC (Miltenyi Biotec), IL-2-PE (Miltenyi Biotec ...
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bioRxiv - Immunology 2020Quote: ... at room temperature (RT) and treated with FcR blocking reagent (Miltenyi Biotec, catalogue number: 130-092-575) for 10 min ...
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bioRxiv - Developmental Biology 2021Quote: ... for 15 min at RT and TAG1+ cells were sorted through MS columns (Miltenyi Biotec, 130-042-201) following the manufacturer’s description.
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bioRxiv - Immunology 2022Quote: ... lung cells were disrupted a second time on a MACs dissociator (Miltenyi Biotec) and processed as above ...
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bioRxiv - Cancer Biology 2024Quote: ... washed 3 times with MACSima™ Running Buffer (Miltenyi Biotec, 130-121-565) before nuclear staining was performed with DAPI (Miltenyi Biotec ...
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bioRxiv - Genetics 2020Quote: ... BM freshly isolated mononuclear cells (MNC) were first stained with Lineage Cell Detection Cocktail-Biotinylated mouse antibody (MACS Miltenyi Biotec). For FACS analysis ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were washed three times with selection buffer and purified on LS-columns (Miltenyi Biotec) according to the manufacturer’s instructions for positive and negative selections.
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bioRxiv - Genomics 2021Quote: ... cells were blocked with 3%BSA diluted in PBS for 1h at RT and then incubated with anti-CD-19-PE antibody (1:50) in 3% BSA-PBS (Miltenyi Biotec) for 1h at RT ...
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bioRxiv - Developmental Biology 2023Quote: ... and incubated the samples for a total of 1h at RT with intermittent pulses of mechanical tissue disruption using the gentleMACSTM tissue homogenizer (Miltenyi Biotec). The resulting homogenate was washed twice using 10 ml of ice-cold 1% BSA/PBS and pelleted for 5min at 800g (4°C) ...
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bioRxiv - Neuroscience 2023Quote: ... Nuclei pellets were washed tree times with one filtration step by MACS pre-separation filter 20μm (Miltenyi Biotec). Nuclei pellets were resuspended in 100 μL of staining buffer (DPBS BSA 2% ...
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bioRxiv - Neuroscience 2021Quote: ... Nuclei pellets were washed tree times with one filtration step by MACS pre-separation filter 20 μm (Miltenyi Biotec). Nuclei pellets were resuspended in 100 μL of staining buffer (DPBS BSA 2% ...
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bioRxiv - Neuroscience 2023Quote: ... Kit (Miltenyi Biotec). Briefly ...
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bioRxiv - Plant Biology 2019Quote: ... Columns were washed either four times with 200 µL of homogenisation buffer and once with wash buffer 2 (Miltenyi Biotec), or three times with 200 µL of homogenisation buffer ...
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bioRxiv - Biochemistry 2021Quote: ... with an incubation time of 3h at room temperature and using secondary labels 0.6 µL anti-c-myc-FITC (Miltenyi Biotec) and 0.25 µL Goat anti-Human IgG Fc PE conjugate (ThermoFisher #12-4998-82) ...
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bioRxiv - Bioengineering 2024Quote: ... Thermo) were washed 3 times with PBS followed by incubation with biotin anti-mouse CD63 antibody (Clone REA563; Miltenyi Biotec) or biotin anti-mouse fibronectin (FN ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were washed in 1%FBS in Dulbecco’s PBS 3 times before straining through 75 µm and 30 µm strainers (Greiner Bio-One and Miltenyi Biotec) in Dulbecco’s PBS ...
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bioRxiv - Immunology 2023Quote: ... Microglia used for qRT-PCR analysis were further sorted using CD11b MicroBeads (Miltenyi Biotec) and immediately lysed for RNA extraction ...
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bioRxiv - Immunology 2020Quote: ... following manufacturer instructions (Dead cell removal kit; Debris removal kit; Miltenyi Biotec) resulting in ~80% of purity and CD3+ T cells were resuspended in 1X PBS with 0.04% BSA ...
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bioRxiv - Bioengineering 2019Quote: ... the cells were washed two times with PBS and the percentage of PI stained cells was determined using a MACSQuant VYB system (Miltenyi Biotec). Data analysis was performed using the FlowJo software.
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bioRxiv - Immunology 2020Quote: ... Cells were then washed three times and cultured with 1 ng/mL rhIL-15 or 20 IU/mL rhIL-2 (Miltenyi Biotec). After 3 days ...
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bioRxiv - Neuroscience 2021Quote: ... Nuclei pellets were washed tree times in staining buffer with one filtration step by MACS pre-separation filter 20 μm (Miltenyi Biotec) to a final resuspension in 300 μl of staining buffer for Malassez cell counting with Trypan blue counterstaining (Trypan Blue solution ...
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bioRxiv - Evolutionary Biology 2022Quote: ... these control populations were measured after at 24 and 48 time periods using the FSC channel of a Macsquant10 (Miltenyi biotec). Flow cytometry was performed with the same setting used in pairwise fitness assays (see above) ...
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bioRxiv - Immunology 2023Quote: ... and incubated at 37°C for total of 30 minutes (15 minutes, 2 times) with rotated using MACSmix Tube Rotator (Miltenyi Biotec). Between and after the two incubations ...
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bioRxiv - Neuroscience 2023Quote: ... Nuclei pellets were washed three times in staining buffer with one filtration step by MACS pre-separation filter 20 μm (Miltenyi Biotec) to a final resuspension in 300 μL of staining buffer for Malassez cell counting with Trypan blue counterstaining (Trypan Blue solution ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were washed three times with selection buffer and labeled with 10 μL streptavidin MACS microbeads (Miltenyi Biotec, 130-048-102) (sufficient for 1e7 cells) ...
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bioRxiv - Plant Biology 2020Quote: ... the μMACS c-myc Isolation Kit and μMACS HA Isolation Kit (Miltenyi Biotec) were used according to the instructions provided ...
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bioRxiv - Cancer Biology 2021Quote: ... and dissociation kit (Miltenyi Biotec) following the machine dissociation protocols ...
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bioRxiv - Cell Biology 2021Quote: ... c-Kit+ cells were extracted with the CD117 Microbead Kit (Miltenyi Biotec, Bergisch Gladbach, Germany). Cells were cultured in complete StemPro-34 serum-free medium (Gibco/Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... [26] using the B cell enrichment kit (Pan B Cell Isolation Kit, Miltenyi Biotec, EUA), according to the manufacturer’s instructions ...