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Citations for Miltenyi Biotec :
1 - 50 of 60 citations for V Bottom Microplate 96 well since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... was designed and prepared in 96-well glass bottom plates (Miltenyi Biotec) using a protocol for solid phase reverse transfection as previously described16,42 ...
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bioRxiv - Immunology 2022Quote: ... cells were resuspended in 96-well U-bottom plates and blocked prior to staining with 1 μg/mL anti–CD40 (clone HB14, Miltenyi Biotec) for 30 minutes at 37°C ...
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bioRxiv - Bioengineering 2024Quote: Splenocytes were plated in 96-well U-bottom plates at 106 cells/well in RPMI and stimulated with 1 μg/mL of PepTivator Ovalbumin (Miltenyi Biotec). After 3 hours ...
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bioRxiv - Cancer Biology 2020Quote: ... polyA+ RNA was purified using the 96-well MultiMACS mRNA isolation kit on the MultiMACS 96 separator (Miltenyi Biotec, Germany) from total RNA with on column DNaseI-treatment as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Two hundred thousand T cells per well were cultured in 96-well plates in the presence of T Cell Activation/Expansion Kit (Miltenyi Biotec) for 2 weeks ...
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bioRxiv - Cell Biology 2024Quote: ... where NM is a mixture of 50% [v/v] DMEM:F12 + 50% [v/v] MACS Neuro Media (Miltenyi Biotec 130-093-570) + 0.5x GlutaMax (Thermo 35050061 ...
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bioRxiv - Developmental Biology 2022Quote: ... 1% v/v BSA (Miltenyi Biotec, 130-091-376), 0.5% v/v Triton-X (Sigma-Aldrich ...
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bioRxiv - Systems Biology 2021Quote: ... were stimulated for 3 days in 96 well plate with MACSi-Beads loaded with anti-mouse CD3 and anti-mouse CD28 antibodies (AP beads, Miltenyi Biotec), at a 1:1 proportion ...
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bioRxiv - Evolutionary Biology 2022Quote: ... These samples were each added to 200 µL of filtered M9 salt solution inside the wells of a 96 well plate and BFP+ and YFP+ cellular events were quantified by flow cytometry using a Macsquant10 (Miltenyi biotec). The competition plates were then sealed with Breathe-Easier Sealing Film (Diversified Biotech) ...
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bioRxiv - Immunology 2021Quote: ... 10% FCS (v/v) containing FcR blocking reagent (Miltenyi Biotec) with fluorescently labeled antibodies as indicated in table 2 and incubated for 30 min at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... and 1 % (v/v) Viobility 405/520 Fixable Dye (Miltenyi Biotec). The cell suspension was incubated for 10 min on ice following addition of 0.5 μg of either the anti-dectin-1 antibody (PE anti-mouse CD369 (dectin-1/CLEC7A ...
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bioRxiv - Immunology 2021Quote: ... Multi-96 Columns (Miltenyi Biotec) were placed into a MultiMACS M96 Separator (Miltenyi Biotec ...
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bioRxiv - Plant Biology 2021Quote: ... diluted 1:2000 (v/v) or anti-GFP HRP-coupled (mouse monoclonal, Miltenyi Biotec) diluted 1:5000 (v/v) ...
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bioRxiv - Immunology 2021Quote: ... 1% penicillin-streptomycin (v/v) and 50 ng/ml M-CSF (Miltenyi Biotec, Bergisch Gladbach, Germany) and seeded at a density of 5 x 105 cells per well of a 6-well plate ...
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bioRxiv - Cancer Biology 2022Quote: ... or Annexin V-FITC Kit (Miltenyi Biotec). All data were processed and analyzed with FCS Express 7 Research Edition (De Novo Software ...
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bioRxiv - Cancer Biology 2023Quote: ... annexin V-FITC was from Miltenyi Biotec, Bergisch Gladbach ...
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bioRxiv - Plant Biology 2020Quote: ... 1% (v/v) Nonidet P-40) and incubated for 2 h with anti-green fluorescent protein (GFP) magnetic beads (MACS / Miltenyi Biotec) at 4 °C under rotation ...
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bioRxiv - Microbiology 2022Quote: ... BSA in PBS + 0.2 mM EDTA (FACS buffer) and subsequently resuspended in 100 μl FACS buffer containing 10 % (v/v) FcR blocking reagent (Miltenyi Biotec) and 1 % (v/v ...
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bioRxiv - Microbiology 2020Quote: ... The samples were mounted on in a glass bottom petri dish (Miltenyi Biotec) with a drop of SlowFade Diamond antifade mountant (Thermofisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... which were subjected to annexin V immunoprecipitation (Miltenyi Biotec Annexin V Microbead Immunoprecipitation kit) ...
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bioRxiv - Developmental Biology 2023Quote: ... Then Myelin Removal Beads II (130-96-733, Miltenyi Biotec) were used to remove myelin debris from single-cell suspensions ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell viability was assessed by Annexin V exposure and PI incorporation using the FITC-Annexin V/PI kit from Miltenyi Biotec (#130-092-052) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... the annexin-V microbead kit (Miltenyi Biotec, Cat. 130-090-201) was used for further enrichment of the pelleted PS-containing vesicles ...
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bioRxiv - Cancer Biology 2019Quote: ... and treated immediately with Annexin V coated magnetic beads (Miltenyi Biotec), resulting in a population of cells with a viability >95% ...
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bioRxiv - Neuroscience 2022Quote: ... 8 well chamber slide dishes (Miltenyi Biotec) were coated over night with 1 mg/ml poly-L-lysine in borate buffer (boric acid ...
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bioRxiv - Molecular Biology 2019Quote: Apoptosis was analyzed using Annexin V-FITC apoptosis detection kit (Miltenyi Biotec). MDA-MB-231 and MCF-7 were incubated with recombinant IFNε for 48 h ...
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bioRxiv - Neuroscience 2021Quote: ... and myelin removal beads (Myelin Removal Beads II, 130-96-733, Miltenyi Biotec) were used according to the manufacturer’s protocol to prepare the cells for incubation with microbead-coupled anti-CD11b mAb ...
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bioRxiv - Neuroscience 2021Quote: ... and then myelin removal beads (Myelin Removal Beads II, 130-96-733, Miltenyi Biotec) were used according to the manufacturer’s protocol to prepare the cells for incubation with microbeads-coupled anti CD11b mAb ...
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bioRxiv - Cancer Biology 2020Quote: Apoptosis was examined by flow cytometry using Annexin V-FITC Kit (Miltenyi Biotec, Italy), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Apoptosis assay was performed using Annexin V-FITC Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Open-repertoire T cells (7.5e106 cells per well of a 24 well plate) were stimulated with OKT3 (50 ng mL−1, Miltenyi Biotec, Cat 130-093-387) and rhIL-2 (300 IU mL−1 ...
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bioRxiv - Immunology 2023Quote: ... 5 % CO2 after which migrated cells in the bottom chamber were collected and acquired on a MACSQuant Analyzer 10 Flow Cytometer using the MACSQuantify software (Miltenyi Biotec). The percentage of specific migration was calculated by dividing the number of cells migrated to the lower well by the total cell input (50 µl cell suspension directly measured on the MACSQuant Flow Cytometer).
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bioRxiv - Microbiology 2023Quote: MEFs were seeded at 5.104 cells per well in 24-well plates one day before stimulation and treated with 300 IU/mL IFNα (Miltenyi Biotec Cat#130-093-131) and incubated for up to 24 hours.
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bioRxiv - Cancer Biology 2020Quote: ... the viability of THP1 was evaluated by Annexin V/Propidium Iodide (PI) assay (Miltenyi Biotec, Italy). Cells in suspension were collected and pooled with adherent cells treated with Trypsin/EDTA (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... We incubated with 5 µL of Annexin V-conjugated FITC (MACS Miltenyi Biotec, Lab Systems SA) at room temperature in darkness for 20 min ...
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bioRxiv - Cell Biology 2021Quote: ... stained with Annexin V-GFP/PI and viability was analysed by flow cytometry (MACSQuant, Miltenyi Biotec, Germany). To analyse the long-term survival ...
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bioRxiv - Cell Biology 2021Quote: ... and v) 0.8 x 106 iPSC resuspended in 157.5 µl of StemMACS iPS-Brew XF (Miltenyi Biotec) medium with 5 μ(Peprotech ...
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bioRxiv - Immunology 2023Quote: ... and FcgR block (0.5 µl/well, FcR Blocking Reagent mouse, Miltenyi Biotec) for 15 min at RT in the dark ...
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bioRxiv - Immunology 2024Quote: ... wells were stained in cold MACS buffer (Miltenyi Biotec, Cat. No.130-091-221) at 4°C for 30 minutes ...
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bioRxiv - Immunology 2021Quote: ... with 4000 cells/well in serum free medium (SFM) containing 10 ng/ml BMP4 (Miltenyi Biotec), 10 ng/ml bFGF (Peprotech) ...
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bioRxiv - Immunology 2024Quote: ... Sigma-Aldrich)-coated tissue culture treated 24 well plates and treated with IFN-γ (1ng/ml, Miltenyi Biotec) and TNFα (1ng/ml ...
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bioRxiv - Cancer Biology 2023Quote: Apoptosis analysis was evaluated by staining cells with Annexin V-FITC and Propidium Iodide (PI) (130-092-52, Miltenyi Biotec, Bergisch Gladbach, Germany), according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... 100,000 cells per well were activated with anti-human TransAct (1:100, Miltenyi Biotec 130-111-160) in T cell medium (10 % FCS ...
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bioRxiv - Immunology 2023Quote: ... the cells were washed with PBS and resuspended in 50 µl of 1X annexin binding buffer containing 2 µl of FITC-annexin V (Miltenyi Biotec, 130-092-052). Cells were then incubated at room temperature for 30 min in the dark ...
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bioRxiv - Immunology 2021Quote: ... or overnight with 1 μg/peptide per well of spike associated (Cat: 130-127-951, Miltenyi Biotec Peptivator) peptides at 37°C ...
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bioRxiv - Immunology 2023Quote: ... NK cells were isolated from respective wells using NK cell isolation kit (Miltenyi biotec; Cat: 130-092-657) following standard protocol and plated at a concentration of 2×105 cells per well of a seahorse XFe96 assay plate in seahorse XF DMEM media (Agilent ...
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bioRxiv - Immunology 2023Quote: ... a 24-well plate was pre-coated with anti-CD3 (OKT3) and anti-CD28 antibodies (15E8) (Miltenyi Biotec) at 1μg/mL on day 0 ...
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bioRxiv - Bioengineering 2023Quote: ... 25 µl of each sample was incubated with APC-labelled CD63 antibody (Miltenyi Biotec, Germany; 1 nM per well) overnight under dark conditions ...
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bioRxiv - Molecular Biology 2020Quote: PBMC were obtained from healthy donors and were cultured in 48-well plates at a density of 1 × 106/ml in TexMACS (Miltenyi Biotec), 3% human serum (Seralab ...
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bioRxiv - Immunology 2021Quote: ... The right lungs as well as the right hemispheres of the brains were homogenized in 1 ml PBS using a gentleMACS Octo Dissociator (Miltenyi Biotec) and viral RNA was isolated from 140 µl cleared homogenate or BAL fluid using QIAamp Viral RNA Mini Kit (Qiagen) ...