Labshake search
Citations for Miltenyi Biotec :
1 - 50 of 1670 citations for Two Hybrid System Construction kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2020Quote: ... Two magnetic-based cell sorting (MACS) isolation kits were used to purified either neutrophils (Miltenyi Biotec) and monocytes ...
-
bioRxiv - Bioengineering 2019Quote: ... the cells were washed two times with PBS and the percentage of PI stained cells was determined using a MACSQuant VYB system (Miltenyi Biotec). Data analysis was performed using the FlowJo software.
-
bioRxiv - Immunology 2023Quote: ... naïve CD8 T cells were isolated from the LNs of NY 8.3 NOD mice using two rounds of the naïve 8.3 CD8 T cell isolation kit (Miltenyi Biotec) with LS columns (Miltenyi Biotec) ...
-
T-cell development and activation in humanized mice lacking mouse major histocompatibility complexesbioRxiv - Immunology 2024Quote: ... CD34+ cells were positively selected through two consecutive runs using immune magnetic beads kits (Miltenyi Biotec, Bergisch-gladbach, Germany) and cryopreserved ...
-
bioRxiv - Immunology 2023Quote: ... 8.3 CD8 T cells were isolated from the LNs of non-diabetic NY 8.3 NOD female mice using two rounds of the CD8 T cell isolation kit (Miltenyi Biotec) with LS columns (Miltenyi Biotec) ...
-
bioRxiv - Neuroscience 2023Quote: ... ipsilateral perilesional cortex and hippocampus from Sham and CCI mice were rapidly microdissected and a single cell suspension was prepared from the combined tissues (pooled tissue from two mice) using enzymatic digestion (Neural Tissue Dissociation Kit; Miltenyi Biotec) in combination with a gentleMACS Dissociator ...
-
bioRxiv - Immunology 2023Quote: ... B cells were isolated from PBMCs through two rounds of magnetic enrichment using the human pan B cell isolation kit (Miltenyi Biotec) according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2024Quote: Isolation of untouched CD8+ T cells from total PBMCs was performed using a two-step human CD8+ T cell isolation kit (Miltenyi Biotec). All steps were performed following manufacturer instructions ...
-
bioRxiv - Immunology 2020Quote: ... cells were loaded onto two LS columns (Miltenyi Biotec) for magnetic enrichment of CD172a expressing cells ...
-
bioRxiv - Immunology 2023Quote: ... naïve 8.3 CD8 T cells from the LNs of non-diabetic NY8.3 NOD mice were purified using two rounds of the naïve CD8 T cell isolation kit (Miltenyi Biotec, Bergisch Gladbach, Germany) with LS columns (Miltenyi Biotec) ...
-
bioRxiv - Cancer Biology 2020Quote: ... through two consecutive LS columns (Miltenyi Biotec, 130-042-401). Peripheral blood was collected in heparinized tubes and spun down to collect cellular components ...
-
bioRxiv - Immunology 2024Quote: ... and magnetically enriched by two sequential MS columns (Miltenyi Biotec). Frequencies of antigen-responding T cells were determined based on the total cell count of CD154+ T cells after enrichment ...
-
bioRxiv - Systems Biology 2022Quote: Naïve CD8+ T cells were isolated from the peripheral blood of two healthy donors using the Naive CD8+ T Cell Isolation kit (Miltenyi Biotec, 130-093-244) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2023Quote: We isolated human umbilical cord blood (hUCB) CD34+ cells from two patients using the Miltenyi CD34 Microbead Kit and MACS separation column (Miltenyi Biotec, Boston, MA, USA), following the manufacturer’s instructions ...
-
bioRxiv - Immunology 2022Quote: ... we passed the stained cells through two LS columns (Miltenyi Biotec) stacked on top of one another ...
-
bioRxiv - Neuroscience 2021Quote: ... consecutively passed through two MS columns (Miltenyi Biotec, 130-042-201) placed on a OctoMACS™ manual separator and flushed into Eppendorf tubes in 0.5 % BSA in PBS ...
-
bioRxiv - Neuroscience 2021Quote: ... consecutively passed through two MS columns (Miltenyi Biotec, 130-042-201) placed on a OctoMACS™ manual separator and flushed into Eppendorf tubes in 0.5 % BSA in PBS ...
-
bioRxiv - Immunology 2020Quote: ... Two-fold dilutions of the anti-human CD303 BDCA2 antibody (AC144) (Miltenyi Biotec), chimeric CBS004 (Capella Bioscience ...
-
bioRxiv - Immunology 2021Quote: ... Two positive controls for activation were used: PepTivator against cytomegalovirus (Pep-CMV, Miltenyi Biotec), which consisted of 15-mer peptides with 11 amino acids ...
-
bioRxiv - Neuroscience 2024Quote: ... Two runs of enrichment were performed on consecutive MS columns (#130-042-201, Miltenyi Biotec).
-
bioRxiv - Biochemistry 2021Quote: ... Splenic T cells were purified using T cell isolation kits and AutoMacs magnetic separation system (Miltenyi Biotec) and approximately 90% of the purified cells displayed a naïve phenotype as previously described19 ...
-
bioRxiv - Microbiology 2022Quote: ... Scraped cells were homogenized by two dissociation cycles on a gentleMACS Octo Dissociator (Miltenyi Biotec #130095937) using the RNA_02.01 program and 10 gentleMACS M tubes (Miltenyi Biotec #130093236) ...
-
bioRxiv - Immunology 2020Quote: ... each sample was divided into two tubes for either cytokine detection with IFN-γ-FITC (Miltenyi Biotec), IL-2-PE (Miltenyi Biotec ...
-
bioRxiv - Immunology 2020Quote: ... dendritic cells were purified with two rounds of MACS magnetic separation using mouse CD11c microbeads (Miltenyi Biotec) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2021Quote: ... Two brains were digested in each C-tube using program 37_ABDK on the GentleMACS™ OctoDissociator (Miltenyi Biotec) to produce a single cell suspension ...
-
bioRxiv - Genetics 2020Quote: ... following the manufacturer’s instruction for 30 minutes (4 °C) before being passed successively through two LS Columns (Miltenyi Biotec) with three MACs buffer washes ...
-
bioRxiv - Neuroscience 2023Quote: ... with the latter two additionally analyzed by flow cytometry with suitable isotype antibodies (all: Miltenyi Biotec, Bergisch Gladbach, Germany) and unstained controls ...
-
bioRxiv - Developmental Biology 2023Quote: Cell suspension underwent two negative selection steps to deplete myelin (Myelin removal beads II, 130-096-733, Miltenyi Biotec) and CD45-positive cells (CD45 microbeads ...
-
bioRxiv - Neuroscience 2021Quote: ... and processed for generation of single-cell suspensions using the Adult Brain Dissociation Kit and GentleMACS Octo Dissociator system (#130-107-677 and #130-095-937, respectively, Miltenyi Biotec) (Chucair-Elliott et al. ...
-
bioRxiv - Immunology 2021Quote: ... and MACS system (Miltenyi Biotec), as described previously (Nojima et al. ...
-
bioRxiv - Neuroscience 2019Quote: ... The triturated cell suspension was filtered in two stages using a 70 μm cell strainer (Miltenyi Biotec Cat # 130-098-462) and 40 μm pipette tip filter (Bel-Art Cat ...
-
bioRxiv - Microbiology 2021Quote: ... cultures of the two parasite lines for comparison were (i) synchronized to 80% late schizonts using MACS purification columns (Miltenyi Biotec) (ii ...
-
bioRxiv - Developmental Biology 2020Quote: ... Cell clumps and debris were removed by two consecutive rounds of centrifugation 3 minutes at 200xg and filtration through 30 µm cell strainers (Miltenyi Biotec). Additional mechanical dissociation and removal of cell aggregates was achieved by gentle pipetting up and down 5 times using a P200 pipette and Gel Saver tips (QSP) ...
-
bioRxiv - Developmental Biology 2022Quote: ... Remaining clumps of cells and debris were removed via two rounds of centrifugation for 3 min at 200 g followed by filtration through 30 μm cell strainers (Miltenyi Biotec). To ensure all remaining cells aggregates were dissociated ...
-
bioRxiv - Neuroscience 2022Quote: ... The pellet was resuspended in 6 mL MACS+ buffer and evenly distributed between two LS columns (Miltenyi Biotec 130-042-401) in the presence of a magnetic field (generated by the QuadroMACS magnetic separator ...
-
bioRxiv - Systems Biology 2021Quote: ... Single-cell suspensions were obtained by enzymatically digesting the excised tumors using the Tumor Dissociation Kit and gentleMACS C system (Miltenyi Biotec, Auburn, CA). In addition to following the manufacturer’s instructions ...
-
bioRxiv - Immunology 2022Quote: ... Mononucleated cells from PB or BM were then enriched for CD34+ cells on the AutoMacs system using human CD34 MicroBead kit (Miltenyi Biotec, #130-046-702) according to manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: ... with the MACS system (Miltenyi Biotec). After enrichment ...
-
bioRxiv - Physiology 2023Quote: ... Lung ECs were then isolated using two step magnetic bead separation (CD45 and CD31 antibody- conjugated microbeads; Miltenyi Biotec Inc., Auburn, CA, USA). For primary SMCs isolation ...
-
bioRxiv - Microbiology 2020Quote: ... to analyze pDCs for 20 min at 4°C to analyze B cells or with two antibodies directed against CD303(BDCA2)-APC (Miltenyi Biotec, #130-097-931) and CD304(BDCA4)-PE (Miltenyi Biotec # 130-112-045) ...
-
bioRxiv - Cancer Biology 2022Quote: ... 2 mM EDTA in PBS) for 15 minutes and after two washes the samples were incubated with anti-biotin-APCVio770 (Miltenyi Biotec #130-113-851), diluted 1:50 in FACS buffer for 15 minutes ...
-
bioRxiv - Cancer Biology 2021Quote: ... and a MACS MultiStand system (Miltenyi Biotec) together with MS columns (Miltenyi Biotec ...
-
bioRxiv - Neuroscience 2023Quote: ... and a MACS separation system (Miltenyi Biotec) as described previously50,51 ...
-
bioRxiv - Neuroscience 2024Quote: ... The brain tissues from two animals were pooled in every group and processed for live single-cell suspension preparation using gentleMACS™ Tissue Dissociator (Miltenyi Biotec, Gaithersburg, MD, USA). The cell suspensions were incubated with CD11b microbeads (Miltenyi Biotec ...
-
bioRxiv - Immunology 2021Quote: ... Using the autoMACS® Pro separation system (Miltenyi Biotec), APC-labeled CD8α+ cells were depleted ...
-
bioRxiv - Physiology 2019Quote: ... on an OctoMACS™Cell Separator system (Miltenyi Biotec). ATMs were cultured for 24h in RPMI-1630 supplemented with 10% FCS and 1% P/S ...
-
bioRxiv - Cancer Biology 2020Quote: ... cells were separated using the MACS system (Miltenyi Biotec). Isolated Lin− breast cancer cells were cultured in Human EpiCult™-B Medium Kit medium (Stem Cell Technologies ...
-
bioRxiv - Neuroscience 2023Quote: ... Kit (Miltenyi Biotec). Briefly ...
-
bioRxiv - Immunology 2021Quote: ... CD14+ cells were sorted with the MACS system (Miltenyi Biotec), according to the manufacturer instructions and seeded at 5×105 cells/mL in RPMI-1640 media with L-glutamine (Lonza ...
-
bioRxiv - Neuroscience 2020Quote: ... CD11b microbeads and a column MACS separation system (Miltenyi Biotec). Purified microglia were resuspended in DMEM F12 with 10% fetal bovine serum and 1% PenStrep (Gibco) ...