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Citations for Miltenyi Biotec :
1 - 46 of 46 citations for Serum Free RocketFuel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... in RPMI before resting overnight in serum-free TexMACS medium (Miltenyi Biotec) supplemented with 1% Penicillin/Streptomycin at 37°C in a 96-well plate ...
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bioRxiv - Immunology 2021Quote: ... with 4000 cells/well in serum free medium (SFM) containing 10 ng/ml BMP4 (Miltenyi Biotec), 10 ng/ml bFGF (Peprotech) ...
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bioRxiv - Genomics 2021Quote: ... ESC were adapted to 2i media (serum-free N2B27 medium supplemented with MEK inhibitor PD0325901 [0.4 μM, Miltenyi Biotec] ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were washed twice with serum free medium prior to FACS analyses (MACSQuant Analyzer, Miltenyi Biotec, Bergisch Gladbach, Germany). A forward- and side-scatter gate was set to exclude dead cells with forward-scatter (FSC ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then washed in PBS and cultured for 5 days in serum-free DMEM containing 50 ng/ml BDNF (Miltenyi Biotec Cat# 130-096-285 ...
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bioRxiv - Immunology 2020Quote: ... plated in 6-well culture plates at a concentration of 5 × 106 cells/ml in 1 ml of serum-free TexMACS medium (Miltenyi Biotec) and left overnight (5 % CO2 ...
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bioRxiv - Cancer Biology 2020Quote: ... MSC were grown from the fragments retained in the cell strainers in serum-free StemMACS MSC Expansion Media XF (Miltenyi Biotec, Bergisch Gladbach, Germany) supplemented with 1% penicillin/streptomycin ...
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bioRxiv - Neuroscience 2021Quote: ... 0,5% Bovine Serum Albumin (Miltenyi Biotec). After Fc blocking (BD Biosciences ...
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bioRxiv - Genomics 2022Quote: ... supplemented with 0.04% bovine serum albumin (BSA, Miltenyi Biotec), and multiplexed in eight pools according to a pre-established study design (Supplementary Fig ...
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bioRxiv - Immunology 2023Quote: ... containing bovine serum albumin (Miltenyi Biotec, Bergisch Gladbach, Germany). 20 mL of diluted blood was gently layered on top of 15 mL of Histopaque®-1077 (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2021Quote: ... 1% bovine serum albumin (BSA, Miltenyi Biotec, 130-091-376) and 5 ng/mL luteinizing hormone from human pituitary (LH ...
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bioRxiv - Bioengineering 2021Quote: ... 1% bovine serum albumin (BSA, Miltenyi Biotec, 130-091-376) and 5 ng/mL luteinizing hormone from human pituitary (LH ...
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bioRxiv - Cell Biology 2020Quote: ... iPSCs were cultured under feeder-free conditions in StemMACs iPS-Brew (Miltenyi Biotec) on vitronectin-coated 6-well plates with daily medium changes ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 0.5% MACS® bovine serum albumin Stock Solution (Miltenyi Biotec). Labelled cells were sorted using a BD FACS ARIA II flow cytometer (BIOSIT CytomeTRI technical platform ...
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bioRxiv - Molecular Biology 2020Quote: ... 3% human serum (Seralab) +20 ng/ml human recombinant IL-2 (Miltenyi Biotec) and activated with TransAct reagent (Miltenyi Biotec) ...
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bioRxiv - Microbiology 2021Quote: ... Plates were blocked with 3% Bovine serum albumin (BSA, Miltenyi Biotec #130-091-376) in PBS for 1 hour ...
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bioRxiv - Immunology 2019Quote: ... filtered human AB serum then subjected to gentle mechanical dissociation using gentleMACS (Miltenyi Biotec) followed by short 20 min enzymatic dissociation at 37°C using a very low concentration of Liberase TL (0.042 mg/ml ...
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bioRxiv - Immunology 2024Quote: ... 5% human AB serum (Biowest) and 40 IU/ml of IL-2 (Miltenyi Biotec). 24 hours later ...
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bioRxiv - Bioengineering 2024Quote: ... B cells were neutralized with Ca2+/Mg2+ free DPBS with 0.5% BSA (Miltenyi Biotec, 130-091-376) and 2 mM EDTA and then isolated using mouse B cell purification kit (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: ... cells were washed twice by centrifugation with PBS with 0.04% bovine serum albumin (BSA; Miltenyi Biotec), then lysed with 45 µl chilled Lysis Buffer ...
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bioRxiv - Cell Biology 2021Quote: ... GFP-EXO70B2 and free-GFP as a control were isolated using the μMACS GFP-tagged protein isolation kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... After several washing with 1×DPBS containing 0.04% bovine serum albumin (BSA, Miltenyi Biotec, 130-091-376), gastric tumor tissues single cells were acquired with digestive enzymes containing 200 μL Enzyme H ...
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bioRxiv - Immunology 2020Quote: ... in 5% human serum RPMI medium in the presence of 1µg/ml anti-CD40 (HB14, Miltenyi Biotec). After 3 hrs of specific peptide stimulation ...
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bioRxiv - Cancer Biology 2023Quote: ... and 2.5% fetal bovine serum (Hyclone, Logan, Utah, USA)] using gentleMACS Dissociator (Miltenyi Biotec, Bergisch Gladbach, Germany). After the completion of 37_h_TDK_1 program ...
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bioRxiv - Immunology 2024Quote: ... in 5% human serum RPMI medium in the presence of 1µg/ml anti-CD40 (HB14, Miltenyi Biotec). After 3 to 4 hrs of specific peptide stimulation ...
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bioRxiv - Cell Biology 2022Quote: iPSCs were maintained in a feeder-free format on Geltrex-coated 6-well plates using StemMACS iPS-Brew XF media (Miltenyi Biotec) with daily feeding ...
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bioRxiv - Developmental Biology 2020Quote: ... Protein complexes with TTL3-GFP and free GFP as a control were isolated using the μMACS and MultiMACS GFP Isolation Kits (Miltenyi Biotec, Germany), according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2019Quote: ... All lines were trained over to a feeder-free system with Stem MACS iPS-Brew XF media (Miltenyi Biotec 130-104-368). Lines were tested for mycoplasma using Lookout mycoplasma PCR detection kit (Sigma MP0035) ...
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bioRxiv - Neuroscience 2019Quote: ... All lines were transferred over to a feeder-free system with Stem MACS iPS-Brew XF media (Miltenyi Biotec 130-104-368). Lines were tested for mycoplasma using Lookout mycoplasma PCR detection kit (Sigma MP0035).
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bioRxiv - Genomics 2021Quote: ... CD4+ T cells were then isolated from CD14+ monocyte-free PBMCs using anti-human CD4 antibody attached to microbeads (Miltenyi Biotec #130045101) on a new LS column ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with 10% fetal calf serum and 10 ng/ml granulocyte macrophage colony-stimulating factor (GM-CSF, Miltenyi Biotec) 61 ...
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bioRxiv - Immunology 2021Quote: ... The large airways were removed and approximately ~500mg of airway free tissue was collected in a gentleMACS C-tube (Miltenyi Biotec, Auburn, CA) and 5mL digest buffer was added and placed on a gentleMACS octo dissociator for programmed runs ...
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bioRxiv - Cell Biology 2021Quote: ... 0.5% heat-inactivated fetal calf serum (FCS) at pH 7.4 and magnetically sorted with using magnetic microbeads coated with antibodies directed against CD14 (Miltenyi Biotec). Monocytes were then seeded on plastic at 2×106 cells/well in six-well plates in RPMI 1640 (Invitrogen ...
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bioRxiv - Biophysics 2021Quote: ... 0.5% heat-inactivated Fetal Calf Serum (FCS) at pH 7.4 and magnetically sorted with magnetic microbeads coated with antibodies directed against CD14 (Miltenyi Biotec). Monocytes were then seeded on glass coverslips at 1.5 × 106 cells/well in six-well plates in RPMI 1640 (Invitrogen ...
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bioRxiv - Immunology 2022Quote: Dissected and trimmed tumors were washed in pre-cooled RNase-free H2O and transferred into MACS Tissue storage solution (Miltenyi Biotec, 130-100-008). Samples were shipped on ice overnight to Novogene ...
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bioRxiv - Bioengineering 2021Quote: ... and 5% Human AB serum (Gemini) supplemented with 50 units/mL IL-2 every 2 days for all experiments (Miltenyi Biotec).
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bioRxiv - Immunology 2022Quote: ... washed with PBS (Phosphate Buffered Saline) containing 5% FBS (Fetal Bovine Serum) then labeled with anti-PE microbeads (Miltenyi Biotec Inc.) for 15 minutes at 4°C ...
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bioRxiv - Synthetic Biology 2023Quote: ... supplemented with 10% Human AB serum (Gemini Bio Products) with the addition of 100 U/ml human IL-2 IS (Miltenyi Biotec), 1 ng/ml IL-15 (Miltenyi Biotec) ...
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bioRxiv - Molecular Biology 2024Quote: ... program and cultured in TexMACS supplemented with 3% human serum (HS) (Life Science Production) and interleukin-2 (IL-2) (Miltenyi Biotec) for 24 hours ...
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bioRxiv - Neuroscience 2023Quote: ... cells were resuspended in PBS with 0.5% of Bovine Serum Albumin (BSA, pH 7.2) and incubated for 15 min at 4 °C with myelin removal beads II (Miltenyi Biotec, Cologne, Germany) for myelin and debris removal ...
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bioRxiv - Biophysics 2023Quote: ... buffer (2 mM EDTA and 0.5% fetal calf serum (FCS)) and microglia were isolated by positive selection using CD11b microbeads (Miltenyi Biotec, Gladbach, Germany), following the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... The live cell fraction was pelleted and resuspended in 1X PBS with 0.04% bovine serum albumin (BSA, Miltenyi Biotec # 130-091-376) to a final concentration of 1 × 106 cells/mL (1,000 cells per μL).
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bioRxiv - Neuroscience 2022Quote: ... The remaining pellet was resuspended in 1.25 mL of Red Blood Cell Remover Solution and incubated for 10 minutes at 4 °C before the addition of 12 mL of 0.5 % bovine serum albumin (BSA, Miltenyi Biotec, # 130-091-376) diluted in DPBS ...
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bioRxiv - Neuroscience 2022Quote: ... the cells were centrifuged and suspended in chilled Dulbecco’s phosphate-buffered saline / bovine serum albumin and run through a magnetic separator column (MiniMACS Separator; Miltenyi Biotec, 130-042-102). The flow-through fraction ...
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bioRxiv - Neuroscience 2024Quote: ... The supernatant was coupled with Anti-tomm22 micro-Beats (30min at 4° C) to remove free mitochondria through Magnetic-assisted cell sorting (MACS) (Miltenyi Biotec, 130-096-946, 130-042-401). The mitochondria-depleted flow-through was centrifuged at 13,000 × g for 10 min at 4° C to pellet synaptosomes (100ul of PBS resuspension ...
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bioRxiv - Cell Biology 2022Quote: Purified milk and serum EVs were subjected to multiplex bead-based analysis by flow cytometry using the human MACSPlex Exosome Kit (Miltenyi Biotec, 130-108-813). EV samples were processed overnight in the MACSPlex Filter Plate as described in the manufacturer’s instructions and in a previously published paper on a systematic evaluation of the assay19 ...