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Citations for Miltenyi Biotec :
1 - 50 of 493 citations for SARS CoV 2 Spike Glycoprotein S2 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 Spike peptides (PepTivator SARS-CoV-2 Prot_S, Miltenyi Biotec) were added at a final concentration of 0.6 nmol/ml in the presence of 1 μ (total cell culture volume ...
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bioRxiv - Immunology 2021Quote: ... The cells were incubated for 18 h at 37 °C at 1.5×106 cells per well with 2 μg/ml of SARS-CoV-2 Spike protein peptide pool (PepTivator® SARS-CoV-2 Prot_S, Miltenyi Biotec, Germany). Cells were stained with anti-CD8α (APC) ...
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bioRxiv - Immunology 2021Quote: ... Cells were cultured for 18 h at 5×105 cells per well with 2 μg/ml of a peptide pool consisting mainly of 15-mers (overlapping by 11 amino acids) covering the immunodominant sequence domains of the SARS-CoV-2 Spike protein (PepTivator® SARS-CoV-2 Prot_S; Miltenyi Biotec, Germany). The number of spots was determined using an automatic ELISPOT reader and image analysis software (CTL-ImmunoSpot® S6 Micro Analyzer ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 spike PepTivator® protein pools (Miltenyi Biotec, Gladbach, GER) were used to test T cell responses against full spike proteome ...
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bioRxiv - Immunology 2023Quote: ... and the membrane glycoprotein (PepTivator® SARS-CoV-2 Prot_M) were obtained from Miltenyi Biotec. The PepTivator® Peptide Pools are constituted by peptides of 15 amino acid length with 11 amino acid overlap ...
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bioRxiv - Microbiology 2023Quote: Splenocytes or lung leukocytes were incubated with SARS-CoV-2 spike peptide pools (1μg/ml, Miltenyi Biotec) for 6 h in the presence of GolgiPlug (BD Bioscience) ...
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bioRxiv - Immunology 2020Quote: ... covering the N-terminal S1 domain of the Spike protein of SARS-CoV-2 (Miltenyi Biotec, Auburn, CA) at 0.2 μg/mL and 0.5 μg/mL per peptide ...
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bioRxiv - Bioengineering 2022Quote: ... or the PepTivator SARS-CoV-2 Prot_N peptide mix (covering the complete sequence of the SARS-CoV-2 N protein; both Miltenyi Biotec). As controls splenocytes were either left unstimulated or activated polyclonally with 12.5 µg/mL phytohemagglutinin (PHA) ...
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bioRxiv - Immunology 2024Quote: ... MP1 and SARS-CoV-2 Prot_S (SPIKE) (peptides pools of 15 amino acids length with 11 amino acid overlap, Miltenyi Biotec) in 5% human serum RPMI medium in the presence of 1µg/ml anti-CD40 (HB14 ...
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bioRxiv - Microbiology 2022Quote: ... 3 × 105 cells harvested from the spleen or cervical lymph nodes were incubated for 16 h in the presence of a peptide pool derived from the S protein of SARS-CoV-2 (a mixture of PepTivator SARS-CoV-2 Prot_S, S1, and S+; Miltenyi Biotec, Bergisch Gladbach, Germany). After washing the cells with PBS ...
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bioRxiv - Immunology 2020Quote: Pools of lyophilized peptides covering the immunodominant sequence of the spike glycoprotein and the complete sequence of the membrane glycoprotein of SARS-CoV-2 (15-mer sequences with 11 amino acids overlap) were obtained from Miltenyi Biotec (Thieme et al., 2020) resuspended and stored according to the manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... or 1 µg/mL SARS-CoV-2 Prot_S (Miltenyi Biotec), no antigen was added to control wells ...
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bioRxiv - Immunology 2024Quote: ... PepTivator® SARS-CoV-2 Prot_S-peptide pool (Miltenyi Biotec, consisting of 15-mer sequences with 11 amino acids overlap covering the complete protein coding sequence (aa 5–1273 ...
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bioRxiv - Immunology 2022Quote: ... or specifically using two pools of Spike peptides covering the C- and N-terminal (PepTivator SARS-CoV-2 Prot_S1 and PepTivator SARS-CoV-2 Prot_S+) (Miltenyi Biotec Ref. 130-126-701& 130-126-700), Peptide pools were used at a final concentration of 1 μg/ml each peptide ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and a SARS-CoV-2 S peptide pool (Miltenyi Biotec, Germany) for immune reaction together with 10 μg/ml brefeldin A ...
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bioRxiv - Immunology 2021Quote: SARS-CoV-2 PepTivator peptide pools (Miltenyi Biotec, Bergisch Gladbach, Germany), mainly consisting of 15-mer sequences with 11 amino acids (aa) ...
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bioRxiv - Immunology 2024Quote: ... AVX/COVID-12 or 30 nM of a mixture of 10-15 amino acid long peptides derived from the SARS-CoV-2 spike protein (PepTivator, Miltenyi Biotec, North Rhine-Westphalia, Germany). In parallel ...
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bioRxiv - Microbiology 2024Quote: ... Splenocytes were stimulated with SARS-CoV-2 S peptide pools (2 μg/ml, Miltenyi Biotec) for 24 h at 37°C ...
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bioRxiv - Microbiology 2024Quote: Splenocytes were incubated with SARS-CoV-2 S peptide pools (1μg/ml, Miltenyi Biotec) for 6 h in the presence of GolgiPlug (BD Bioscience) ...
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bioRxiv - Immunology 2024Quote: ... cells were stimulated with either PepTivator® SARS-CoV-2 Prot_S-peptide pool (Miltenyi Biotec) or S recombinant protein (Sino Biological ...
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bioRxiv - Immunology 2020Quote: ... PBMCs were stimulated with SARS-CoV-2 peptide pools of S and N (2 μg/ml) (Miltenyi Biotec) in the presence or absence of CECs ...
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bioRxiv - Immunology 2021Quote: ... M and N protein pools [1 µg/mL] (PepTivator® SARS-CoV-2 regents; Miltenyi Biotec) in 96-well U-bottom plates at 0,5-1×106 PBMCs per well ...
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bioRxiv - Immunology 2022Quote: ... the PepTivator® SARS-CoV-2 Prot_S B.1.1.529 Mutation Pool (130-129-928, Miltenyi Biotec) and SARS-CoV-2 Spike Glycoprotein B.1.1.529-Omicron (RP30121 ...
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bioRxiv - Immunology 2022Quote: ... PepTivator® SARS-CoV-2 Prot_S B.1.1.529 WT Reference Pool (130-129-927, Miltenyi Biotec) and SARS-CoV-2 Spike Glycoprotein-crud (RP30020 ...
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bioRxiv - Immunology 2021Quote: ... Splenocytes or lung leukocytes were stimulated in duplicates with SARS-CoV-2 S peptide pools (2 μg/ml, Miltenyi Biotec) for 24 h at 37°C ...
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bioRxiv - Immunology 2021Quote: Splenocytes or lung leukocytes were incubated with SARS-CoV-2 S peptide pools (1μg/ml, Miltenyi Biotec) for 24 h ...
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bioRxiv - Microbiology 2021Quote: ... Splenocytes (0.25 × 106) were stimulated in duplicates with SARS-CoV-2 S- or N-peptide pool (2 µg/ml, Miltenyi Biotec, USA) for 24 hours at 37°C ...
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bioRxiv - Immunology 2021Quote: ... M and N protein pools separately [1 µg/mL each] (PepTivator® SARS-CoV-2 reagents; Miltenyi Biotec), Monensin (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... and PepTivator® SARS-CoV-2 Prot_S Complete peptide pool (Miltenyi Biotec, 15 mers with 11 amino acid overlap) covering the entire SARS-CoV-2 S protein ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then stimulated for 18 hours at 37 °C with SARS-CoV-2 S PepTivator peptides (Miltenyi Biotec) or media alone as a negative control ...
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bioRxiv - Immunology 2022Quote: ... (3) Non-SARS-CoV-2 antigens: Peptide pools of the pp65 protein of human cytomegalovirus (CMV) (Miltenyi Biotec, Gladbach, GER), or HIV-1 gag peptide pools (NIH AIDS Reagent Repository ...
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bioRxiv - Immunology 2022Quote: ... lungs of SARS-CoV-2 infected mice were collected and homogenized with gentle MACS M tubes (Miltenyi Biotec, 130-093-236) in a gentle MACS dissociator (Miltenyi Biotec ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... Lung cells were centrifuged and resuspended with fresh media with 20 μL/mL of PeptTivator® SARS-CoV-2 Prot_S (Miltenyi Biotec) and 5 μg/mL Brefeldin A (BioLegend) ...
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bioRxiv - Immunology 2020Quote: ... PBMCs from 48 healthy individuals (samples taken before the SARS-CoV-2 outbreak) were enriched for CD19+ cells with CD19-microbeads (Miltenyi Biotec). For each individual ...
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bioRxiv - Bioengineering 2023Quote: Lung cells were centrifuged and resuspended with fresh complete RPMI media with 20 μL/mL of PeptTivator® SARS-CoV-2 Prot_S (Miltenyi Biotec) and 5 μg/mL Brefeldin A (Biolegend) ...
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bioRxiv - Microbiology 2022Quote: ... and stimulated with either SARS-CoV-2 S-trimer (10-100 µg/mL) or SARS-CoV-2 PepTivator® Peptide S and NP protein Pools (10 µg/mL each, Miltenyi Biotec) for 72 h at 37°C ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... Cells were centrifuged and resuspended with fresh media with 40 μL/mL of PeptTivator® SARS-CoV-2 Prot_S (Miltenyi Biotec, Cat# 130-126-700), 1 μL/mL monensin (BioLegend) ...
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bioRxiv - Immunology 2021Quote: ... and the respective SARS-CoV-2 peptide pool (0.6 nmol/peptide, S and N pools from Miltenyi Biotec, 130-126-701 and 130-126-699). Unstimulated samples were used for subtraction of background cytokine production ...
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bioRxiv - Microbiology 2020Quote: ... nucleocapsid(N) or membrane glycoprotein (M) sequence (Miltenyi Biotec, Bisley, UK) or 5 μg/ml staphylococcal enterotoxin b (SEB ...
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bioRxiv - Immunology 2020Quote: ... by adding anti-CD45RA biotin (Miltenyi Biotec, Clone REA1047, 2 uL per stain) to the antibody cocktail ...
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bioRxiv - Cancer Biology 2024Quote: ... anti-c-myc-biotin and anti-biotin-PE (Miltenyi Biotec, 130-124-899 and 130-111-068 ...
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bioRxiv - Cancer Biology 2022Quote: ... Biotin (Miltenyi Biotec) followed by incubation with APC-anti biotin Antibody ...
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bioRxiv - Developmental Biology 2020Quote: ... Samples resuspended in 2% FBS/PBS solution were blocked with mouse Fc Block (Miltenyi Biotec) for 5 minutes on ice ...
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bioRxiv - Immunology 2021Quote: ... Lin–biotin (Lineage Cell Detection Cocktail-Biotin, Miltenyi Biotec, #130-092-613), Streptavidin–PE (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... CD11c-biotin and B220-biotin antibodies for 20 min at 4°C and labeled with anti-biotin magnetic beads (Miltenyi Biotec) for 15 min at 4°C ...
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bioRxiv - Immunology 2022Quote: ... anti-CD235-biotin (homemade) and anti-biotin Microbeads (Miltenyi Biotec, 130-090-485). Human postnatal thymus was processed as previously described 36.
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bioRxiv - Developmental Biology 2021Quote: ... Positive selection was subsequently applied with an antibody against biotin-conjugated integrin α7-biotin and anti-biotin MicroBeads (Miltenyi Biotec, 130-090-485), followed by MS columns (Miltenyi Biotec ...
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bioRxiv - Immunology 2019Quote: ... negatively selected B cell fractions with CD23 Biotin and anti-Biotin Microbeads (Miltenyi Biotec)
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bioRxiv - Neuroscience 2024Quote: ... with Fc receptors blocking prior to Anti-ACSA-2 MicroBeads (Cat# 130-097-678, Miltenyi Biotec, Germany). Incubation was carried out for 15 minutes in the refrigerator at 4℃ ...
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bioRxiv - Genomics 2020Quote: ... anti-biotin microbeads (Miltenyi Biotec) and LS columns (Miltenyi Biotec ...