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Citations for Miltenyi Biotec :
1 - 50 of 878 citations for Recombinant Human CD4 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells were purified from human PBMCs by using human CD4 MicroBeads (Miltenyi Biotec). CD4+ CD25Lo CD127Hi Tconv cells and CD4+ CD25Hi CD127Lo Treg cells were sorted by flow cytometry and stimulated in 96-well cell-culture plates with three times the number of Dynabeads M-450 Tosylactivated beads (Thermo Fisher Scientific ...
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Optimization of AAV6 transduction enhances site-specific genome editing of primary human lymphocytesbioRxiv - Molecular Biology 2021Quote: ... Human CD4+ T cells were isolated by positive magnetic selection using human CD4 MicroBeads kit (Miltenyi Biotec, Bergisch Gladbach, Germany) per manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... human recombinant IL-2 (Miltenyi Biotec) was added to the medium every two days from day 2 on (100 U/ml) ...
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bioRxiv - Cancer Biology 2023Quote: ... recombinant human Flt3-ligand (Miltenyi Biotec), recombinant human IL-6 (Miltenyi Biotec) ...
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bioRxiv - Cancer Biology 2023Quote: ... recombinant human IL-6 (Miltenyi Biotec), recombinant human TPO (PeproTech) ...
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bioRxiv - Microbiology 2022Quote: ... and CD4+ T cells were isolated using human CD4+T cell isolation kit (Miltenyi Biotec). Purified CD4+T cells were washed and stained with 3 µM CFSE (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: ... Primary human CD4+ T cells were isolated by untouched CD4+ T cell isolation kit (Miltenyi Biotec). Human CD4+ T cells were activated by Dynabeads Human T-Activator CD3/CD28 (ThermoFisher ...
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bioRxiv - Microbiology 2023Quote: ... CD4+ T cells were isolated by positive selection (using magnetic CD4 human MicroBeads from Miltenyi Biotec). Cells were activated with CD3/CD28 agonists (T Cell transact ...
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bioRxiv - Plant Biology 2021Quote: ... To control for recombinant bacterial MCO expression anti-His-HRP (Miltenyi Biotec) was used (at a dilution of 1:10000 ...
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bioRxiv - Immunology 2023Quote: Human CD4+ T cells were isolated by employing a CD4+ T cells isolation kit (Miltenyi Biotec kit) following the provider’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Naïve CD4+ T cells were isolated using Human Naïve CD4+ T Cell Isolation Kit II (Miltenyi Biotec). CD14+ monocytes were isolated using Human CD14 Microbeads (Miltenyi Biotec) ...
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bioRxiv - Immunology 2021Quote: ... CD4 T cells were purified by negative selection using the human CD4 T Cell Isolation Kit (Miltenyi Biotec). T cells were activated with T cell activation/expansion CD2/3/28 beads at a ratio of 1:2 bead to cells (Miltenyi Biotec) ...
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bioRxiv - Microbiology 2021Quote: Purified rhesus and human CD4+ T cells were isolated from PBMCs using magnetic MACS CD4 MicroBeads (Miltenyi Biotec), as previously described (35) ...
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bioRxiv - Genomics 2021Quote: ... CD4+ T cells were then isolated using magnetic column separation (Miltenyi Biotec, CD4+ T cell isolation kit, human); 17.7 million CD4+ T cells ± 7.2 million were isolated from each subject ...
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bioRxiv - Molecular Biology 2020Quote: ... 3% human serum (Seralab) +20 ng/ml human recombinant IL-2 (Miltenyi Biotec) and activated with TransAct reagent (Miltenyi Biotec) ...
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bioRxiv - Microbiology 2020Quote: Human primary CD4+ T cells are obtained from peripheral blood with CD4 T cell isolation kit II (Miltenyi Biotec). Unactivated CD4+ T cells were maintained in complete RPMI medium containing 50 U/ml interleukin 2 (IL-2 ...
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bioRxiv - Immunology 2022Quote: ... using the human CD4+ T cell isolation kit (MACS Miltenyi Biotec). Isolated T helper cell purity was checked by flow cytometry CD4 staining and they were then cultured in CD3 (5µg/ml ...
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bioRxiv - Immunology 2022Quote: ... recombinant human IL-2 (100 IU/ml, Miltenyi Biotec), PHA (0,25 µg/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 50ng/ml recombinant human SCF (Miltenyi Biotec), recombinant human Flt3-ligand (Miltenyi Biotec) ...
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bioRxiv - Immunology 2022Quote: RM CD4+ T cells were isolated from PBMCs using the non-human primate CD4+ T cells isolation kit (Miltenyi Biotec), activated using the NHP T-Cell Activation/Expansion kit (Miltenyi Biotec ...
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bioRxiv - Microbiology 2023Quote: ... Enrichment of primary CD4+ T cells was performed using the EasySep™ Human CD4+ T Cell Enrichment Kit (Miltenyi Biotec) according to the manufacturer’s manual ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T cells were isolated from PBMC using Human CD4+ T Cell Isolation Kits (Miltenyi Biotec, Inc., Bergisch Gladbach, Germany). Naïve CD4+ T cells were isolated using Human Naïve CD4+ T Cell Isolation Kit II (Miltenyi Biotec) ...
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bioRxiv - Immunology 2022Quote: ... MACS CD4+CD25+CD127dim/− Regulatory T cell isolation Kit human (Miltenyi Biotec) was used for the stimulation experiments with IL-27 and qPCR analysis in enriched Tregs from patients with IBD ...
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bioRxiv - Immunology 2020Quote: ... Human CD4+ T-cells were isolated from peripheral blood lymphocytes using naïve CD4+ T-cell isolation kit from Miltenyi Biotec (Germany). The blood samples were obtained from Rheumatology Clinic at Saint Louis University with informed consent ...
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bioRxiv - Microbiology 2022Quote: ... density centrifugation and primary CD4+ T cells were purified by negative selection of CD4+ cells using magnetic beads (CD4+ T cell isolation kit, human; Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... CD4+ T cells were obtained via negative selection from PBMCs using a human CD4+ T-cell isolation kit (MACS Miltenyi Biotec, #130-096-533 ...
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bioRxiv - Microbiology 2021Quote: ... 1x105 HEK-293T cells were mixed at a 1:1 ratio in 96-well plates with primary CD4+ T lymphocytes freshly isolated from healthy donors by negative selection (CD4+ T-Cell Isolation Kit II, human, Miltenyi Biotec). Viral transfer was assessed after 24 hours of incubation at 37°C in permeabilized (FIX & PERM Cell Permeabilization kit ...
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bioRxiv - Immunology 2022Quote: ... Recombinant human transforming growth factor β1 (premium grade, Miltenyi Biotec) was diluted and used at the indicated concentrations.
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bioRxiv - Neuroscience 2023Quote: ... and human recombinant TGF-β1 50 ng/mL (Miltenyi Biotec).
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bioRxiv - Genomics 2021Quote: ... CD4+ T cells were then isolated from CD14+ monocyte-free PBMCs using anti-human CD4 antibody attached to microbeads (Miltenyi Biotec #130045101) on a new LS column ...
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bioRxiv - Immunology 2021Quote: ... T cells were selected using human CD4 microbeads and MS columns (Miltenyi Biotec). Procedure was carried out according to manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... PE-Vio770 labeled anti-human CD3 and Vioblue labeled anti-human CD4 (all from Miltenyi Biotec, Bergisch Gladbach, Germany) for 10 minutes at room temperature ...
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bioRxiv - Pathology 2021Quote: ... Human recombinant OSM was added at 100 ng/ml (Miltenyi Biotec). Cells were cultured for 14 days at 37°C in 5% CO2 atmosphere ...
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bioRxiv - Developmental Biology 2022Quote: ... 20 ng/mL human recombinant FGF-2 (all from Miltenyi Biotec)) ...
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bioRxiv - Developmental Biology 2024Quote: ... 20 ng/mL human recombinant FGF-2 (all from Miltenyi Biotec)) and growth factor-reduced Matrigel (Corning ...
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bioRxiv - Immunology 2024Quote: ... with 30 U/ml of recombinant human IL-2 (Miltenyi Biotec). T cells were cultured in AIMV (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... CD4+ T cells were obtained via negative selection from PBMCs using a human CD4+ T-cell isolation kit (MACS Miltenyi Biotec, #130-096-533). Per 1 × 107 total cells ...
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bioRxiv - Cancer Biology 2020Quote: ... and 5ng/ml recombinant human GM-CSF (Miltenyi Biotec, 130-093-866). HEK293T cells (ATCC CRL-3216 ...
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bioRxiv - Immunology 2022Quote: ... and 100IU/ml recombinant human IL-2 (Miltenyi Biotec, Bergisch Gladbach, Germany) were added ...
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bioRxiv - Immunology 2020Quote: ... We magnetically isolated all CD4+ T cells from each sample using a human CD4+ T cell negative isolation kit (Miltenyi biotec, Cat #130-096-533) per manufacturer instructions ...
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bioRxiv - Immunology 2019Quote: ... CD4 T cells were isolated from healthy human donor PBMCs by negative selection (Miltenyi Biotec), and activated with plate-bound anti-CD3 (clone OKT3 ...
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bioRxiv - Immunology 2022Quote: ... CD8 or CD4 T cells were purified from human PBMCs using isolation kits (Miltenyi Biotec) following manufacturer’s instruction ...
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bioRxiv - Genomics 2022Quote: ... and 40 U/ml recombinant human IL-2 (Miltenyi Biotec, #130-097-745) for 2 days prior to flow cytometry analysis.
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bioRxiv - Genomics 2022Quote: ... and 40 U/ml recombinant human IL-2 (Miltenyi Biotec, #130-097-745). HEK293FT cells were a gift from the Salk viral vector core and were cultured in DMEM (high glucose ...
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bioRxiv - Neuroscience 2019Quote: ... human recombinant anti-CD11b/c (1:50) (#130-120-288, Miltenyi Biotec, Germany). Secondary antibodies ...
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bioRxiv - Neuroscience 2023Quote: ... recombinant human Interferon gamma1b (IFNγ1b) was purchased from Miltenyi Biotec (130-096-481). Exon1-mHTT97Q-eGFP and Exon1-wtHTT25Q-eGFP cloned in pcDNA3.1 plasmids were kindly provided by Dr ...
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bioRxiv - Immunology 2022Quote: ... supplemented with 100 ng/mL of recombinant human flt3 ligand (Miltenyi Biotec, Auburn, CA), stem cell factor (SCF ...
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bioRxiv - Immunology 2021Quote: ... Anti-Biotin multi-sort and human CD4+CD45RO+ isolation kits were purchased from Miltenyi Biotec (Auburn, CA). PE+ cell ...
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bioRxiv - Microbiology 2021Quote: ... and differentiated to human monocyte-derived macrophages (hMDMs) by incubation with 100 ng/ml of recombinant human macrophage colony-stimulating factor (rhMCSF, Miltenyi Biotec) for 6 days at 37°C with 5% CO2 in a humidified atmosphere ...
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bioRxiv - Immunology 2020Quote: ... plus 10ng/ml recombinant human IL-2 and IL-4 (Miltenyi Biotec, Auburn, CA, USA). Cells were maintained in a humidified incubator at 37°C with 5% CO2 ...