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Citations for Miltenyi Biotec :
1 - 50 of 2102 citations for Protein Kinase A PKA Activity Assay Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... supplemented with 10 µM rho-associated protein kinase inhibitor Y-27632 (Miltenyi Biotec) for the first 24 h after passaging ...
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bioRxiv - Plant Biology 2022Quote: RNA immunoprecipitation (RIP) assays were performed with the μMACS GFP-Tagged Protein Isolation Kit (Miltenyi Biotec) according to manufacturer’s instructions with minor modifications ...
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bioRxiv - Developmental Biology 2021Quote: ... and seeded at a density of 19,000 cells/cm2 on Matrigel in mTeSR1 supplemented with 10 μM Rho-associated protein kinase inhibitor Y-27632 (Miltenyi Biotec). Upon reaching 95% confluence ...
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bioRxiv - Plant Biology 2019Quote: ... RPS5A::mCherry-PDK1.2 were used for Co-IP assays using a µMACS Epitope Tag Protein Isolation Kit (Miltenyi Biotec), with RPS5A::mCherry-PDK1.1 and RPS5A::mCherry-PDK1.2 transgenic lines as controls ...
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bioRxiv - Plant Biology 2022Quote: ... The corresponding supernatant was used for immunoprecipitation assay with anti-FLAG micro beads according to the manufactureŕs instructions (μMACS Epitope Tag Protein Isolation Kit (MACS Miltenyi Biotec)) ...
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bioRxiv - Cell Biology 2021Quote: ... and FLT3-L (Fms-related tyrosine kinase 3 ligand; Miltenyi Biotec). Cells were cultured at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: ... ChIP assay kit (catalog no. 17-295) was from Miltenyi Biotec. Supplements N2 and B27 were purchased from Life Technologies.
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bioRxiv - Cell Biology 2019Quote: The μMACS GFP-tagged protein isolation kit (Miltenyi Biotec) was used to pull down proteins associated with the GFP-tagged protein ...
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bioRxiv - Cell Biology 2022Quote: ... 1 ml of protein extract was mixed with 50 µl of magnetic beads from µMACS™ GFP Isolation Kit (Miltenyi Biotec) and the mixture was incubated on ice while mild shaking ...
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bioRxiv - Immunology 2022Quote: ... we used human IFNγ secretion assay detection kit (PE) (Miltenyi Biotec, #130-054-202) as per the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Apoptosis assay was performed using Annexin V-FITC Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: ... immuno-purification was performed with µMACS GFP Tag Protein Isolation kits (Miltenyi Biotec). 1 mg of solubilized microsomes were loaded on µcolumns ...
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bioRxiv - Plant Biology 2022Quote: ... μMACS™ GFP or μMACS™ DYKDDDDK (FLAG) Protein Isolation Kits (Miltenyi Biotec) were used for immunoprecipitations ...
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bioRxiv - Microbiology 2023Quote: ... The remaining supernatants were adjusted so that they had the same amount of total protein and mixed with 25 μL α-FLAG magnetic bead as instructed in the μMACS Epitope Tag Protein Isolation Kit (Miltenyi Biotec). From here ...
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bioRxiv - Cell Biology 2019Quote: ... For myotube formation assay satellite cells were isolated using Satellite cell isolation kit from Miltenyi Biotec.
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bioRxiv - Microbiology 2019Quote: ... Cells (106) were stained with the ‘Mouse IFN-γ secretion assay detection kit’ (Miltenyi Biotec, Germany) according to the manufacturer’s instructions and IFN-γ secretion was analyzed by flow cytometry.
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bioRxiv - Immunology 2023Quote: ... IFN-γ-secreting cells were detected using IFN-γ Secretion Assay/Detection Kit (PE) (Miltenyi Biotec) according to the manufacturer’s instructions and subsequently phenotypically characterised by flow cytometry.
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bioRxiv - Immunology 2021Quote: ... Cytokines measured from PBMC supernatants were collected after 4 days of incubation with S protein and concentrations were predicted using a standard curve in the LEGENDplex assay (Miltenyi Biotec 741028).
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bioRxiv - Immunology 2020Quote: ... plates were blocked with 1% BSA in PBS solution (Miltenyi Biotec, Bergisch Gladbach, Germany) for two hours at room temperature ...
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bioRxiv - Immunology 2020Quote: ... the cells were labeled with the IFN-γ Catch Reagent (IFN-γ Secretion Assay-Detection Kit, human Miltenyi Biotec) containing bispecific antibodies for CD45 and IFN-γ which was secreted by the stimulated target cells ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: PBMCs were activated in plates coated with 1 μg/mL anti-CD3 mAb (clone: OKT3, Miltenyi Biotec) and cultured in the presence of 3 μg/mL anti-CD28 mAb (clone ...
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bioRxiv - Immunology 2024Quote: IFN-γ production of CD8+ T cells was measured by adapting a commercially available IFN-γsecretion assay kit (Miltenyi Biotec). Briefly ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins were detected with horseradish peroxidase (HRP)-conjugated anti-GFP antibody (1:5,000; Miltenyi Biotec) for one hour at room temperature ...
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bioRxiv - Plant Biology 2021Quote: ... Proteins fused to mCITRINE were immunoprecipitated using the pull-down kit Miltenyi μMacs anti-GFP (Miltenyi Biotec®). Crude extracts were put in 1XSDG supplemented with bromophenol blue and denaturated at 95°C for 5 min while IP samples were directly ready for migration on polyacrylamide gels.
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bioRxiv - Cancer Biology 2020Quote: ... the population of IFNγ secreting cells was identified using the IFNγ Secretion assay detection kit (PE) (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... The general surface marker composition for the sEVs from the three cell sources (HEK293, cbMSC, THP1) was assessed by a multiplex bead-based flow cytometry assay (MACSPlex Exosome Kit, human, Miltenyi Biotec) by using an optimized workflow as described previously.[27] In brief ...
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bioRxiv - Immunology 2020Quote: ... such T cells were selected using a human IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (Miltenyi Biotec, Auburn, CA) following the manufacturer’s instructions as previously described.38–42 Post-4 PBMC sample cryopreserved after monocyte depletion was thawed and cultured overnight in Yssel’s media (Gemini Bio Products ...
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bioRxiv - Immunology 2023Quote: ... purified CD8+ T-cells were coated with mouse IL-2 catch reagent from a mouse IL-2 secretion assay detection kit (Miltenyi Biotec) by incubating T cells in a 20x dilution of IL-2 catch reagent in R10 medium for 15 min on ice ...
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bioRxiv - Immunology 2024Quote: ... Two hundred thousand T cells per well were cultured in 96-well plates in the presence of T Cell Activation/Expansion Kit (Miltenyi Biotec) for 2 weeks ...
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bioRxiv - Immunology 2020Quote: ... and the complete sequence of the nucleocapsid phosphoprotein N and the membrane glycoprotein M (GenBank MN908947.3, Protein QHD43416.1, Protein QHD43423.2, Protein QHD43419.1) (Miltenyi Biotec, Germany). After 5 hours of stimulation ...
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bioRxiv - Immunology 2021Quote: ... M and N protein pools [1 µg/mL] (PepTivator® SARS-CoV-2 regents; Miltenyi Biotec) in 96-well U-bottom plates at 0,5-1×106 PBMCs per well ...
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bioRxiv - Plant Biology 2022Quote: ... Proteins were subsequently detected with horseradish peroxidase (HRP)-conjugated anti-GFP antibody (1:5,000) (Miltenyi Biotec) for 1 hr at room temperature with gentle shaking ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were subsequently detected with horseradish peroxidase (HRP)-conjugated anti-GFP antibody (1:5,000) (Miltenyi Biotec) or HRP-conjugated anti-c-myc antibody (1:5,000 ...
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bioRxiv - Plant Biology 2020Quote: ... Co-immunoprecipitation was conducted using the μMACS™ Epitope Tag Protein Isolation Kits according to manufacturer’s instructions (Miltenyi Biotec), and purified on magnetic μ Column (Miltenyi Biotec) ...
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bioRxiv - Neuroscience 2020Quote: ... Immunoprecipitation (IP) was carried out with the μMACS isolation kits for tagged proteins from Miltenyi Biotec (Bergisch-Gladbach, Germany). The eluates ...
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bioRxiv - Molecular Biology 2020Quote: Immunoprecipitation of uL18L1-3HA and uL18L8-3HA were performed using the μMACS HA-Tagged Protein Isolation Kit (Miltenyi Biotec). Briefly ...
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bioRxiv - Developmental Biology 2021Quote: ... GFP-tagged proteins were magnetically labelled and subsequent isolated using µMACS Isolation Kit (Miltenyi Biotec S.L., Bergisch Gladbach, Germany) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... GFP-EXO70B2 and free-GFP as a control were isolated using the μMACS GFP-tagged protein isolation kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... Multi Tissue Dissociation Kit 1 and gentleMACS Octo Dissociator (Miltenyi Biotec) were used for dissociation of the tissue into single-cell suspension with a reduced amount of enzyme R (25% of the regular amount) ...
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bioRxiv - Immunology 2021Quote: ... M and N protein pools separately [1 µg/mL each] (PepTivator® SARS-CoV-2 reagents; Miltenyi Biotec), Monensin (Biolegend ...
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bioRxiv - Neuroscience 2019Quote: ... plates in StemMACs culture medium (Miltenyi Biotec). The medium was changed every day and the cells were passaged every 5-6 days using PBS without Calcium and Magnesium (Life Technologies).
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bioRxiv - Cancer Biology 2022Quote: ... we separate the epidermal tissue from the dermis and then dissociated to single-cell suspensions by combining mechanical dissociation with enzymatic degradation of the extracellular adhesion proteins using Epidermis Dissociation Kit for Human (130-103-464, MACS Miltenyi Biotec). The dissociated cell suspension was strained with a 40μm filter (BD Falcon) ...
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bioRxiv - Molecular Biology 2020Quote: PBMC were obtained from healthy donors and were cultured in 48-well plates at a density of 1 × 106/ml in TexMACS (Miltenyi Biotec), 3% human serum (Seralab ...
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bioRxiv - Immunology 2020Quote: ... plated in 6-well culture plates at a concentration of 5 × 106 cells/ml in 1 ml of serum-free TexMACS medium (Miltenyi Biotec) and left overnight (5 % CO2 ...
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bioRxiv - Immunology 2022Quote: ... cells were resuspended in 96-well U-bottom plates and blocked prior to staining with 1 μg/mL anti–CD40 (clone HB14, Miltenyi Biotec) for 30 minutes at 37°C ...
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bioRxiv - Bioengineering 2024Quote: Splenocytes were plated in 96-well U-bottom plates at 106 cells/well in RPMI and stimulated with 1 μg/mL of PepTivator Ovalbumin (Miltenyi Biotec). After 3 hours ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were subsequently detected with horseradish peroxidase (HRP)-conjugated anti-GFP antibody (1:5,000) (Miltenyi Biotec #130-091-833), anti-plant actin mouse monoclonal antibody (3T3)-HRP (Abbkine # A01050HRP) ...
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bioRxiv - Cancer Biology 2022Quote: 24-well tissue culture-treated plates were pre-coated with 1 µg/mL anti-CD3 (OKT3) antibody (Miltenyi Biotec 130-093-387) in 1 mL PBS per well ...
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bioRxiv - Microbiology 2020Quote: ... iPSCs were maintained on Geltrex (Thermofisher Scientific)-coated plates in iPS-Brew XF (Miltenyi Biotec) media at 37°C in 5% CO2/5% O2/90% air humidified atmosphere ...
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bioRxiv - Cell Biology 2020Quote: Astrocytes were purified using the Anti-GLAST (ACSA-1) MicroBead Kit (Miltenyi biotec). Briefly ...