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Citations for Miltenyi Biotec :
1 - 50 of 98 citations for Protein Conjugation Labeling since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... followed by labeling with anti-APC microbeads (Miltenyi Biotec). DP and CD8SP thymocytes were depleted by applying the “depleteS” program of AutoMACS Pro (Miltenyi Biotec) ...
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A scalable, GMP-compatible, autologous organotypic cell therapy for Dystrophic Epidermolysis BullosabioRxiv - Bioengineering 2023Quote: ... and FITC-Labeling Reagent (Miltenyi Biotec, Cat # 327806, 1:50 dilution). Composition analysis of enriched iSCs was performed using anti- ITGB4 (BD Cat # 744150 ...
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bioRxiv - Cell Biology 2020Quote: ... followed by magnetic labeling with an Anti-APC MicroBeads kit (Miltenyi Biotec). Cells were then applied to magnetic MS columns (Miltenyi Biotec) ...
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bioRxiv - Neuroscience 2021Quote: ... Antibody labeling was done according to the Microbead kit protocols (Miltenyi biotec) for oligodendrocytes (O4 ...
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bioRxiv - Neuroscience 2020Quote: ... Prior to labeling with anti-AN2 MicroBeads (Miltenyi Biotec, Cat#130-097-170), the cell suspension was incubated with the OPC MACS cultivation medium (MACS Neuro Medium containing MACS NeuroBrew-21 (Miltenyi Biotec ...
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bioRxiv - Systems Biology 2021Quote: ... and followed by a second labeling with Anti-Biotin Microbeads Ultrapure (Miltenyi Biotec) for 15min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... The labeling mix contained B220-FITC (REA755, Miltenyi Biotec®, Bergisch Gladbach, Germany) and CD3-PE (REA641 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... antibody labeling and subsequent isolation using a magnetic anti-APC-Microbead Kit (Miltenyi Biotec). NALM6 cells were cultivated in RPMI 1640 medium (Biowest ...
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bioRxiv - Neuroscience 2020Quote: ... CD45+ immune cells were isolated using magnetic labeling and the autoMACS (Miltenyi Biotec, Auburn, CA). CD45-cells were stored at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... untouched CD4+ T cells were purified from PBMCs with an indirect magnetic labeling system (MACS, Miltenyi Biotec), according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Monocytes were isolated by magnetic labeling with StraightFrom Whole Blood CD14 Microbeads and AutoMacs-directed selection (Miltenyi Biotec). Primary human macrophages were differentiated ex vivo from monocytes by culturing the monocytes in IMDM supplemented with 10% heat-inactivated FBS ...
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bioRxiv - Neuroscience 2023Quote: ... antibody labeling and boosting step was performed with the addition of CD11b (1:50) (Miltenyi Biotec, 130-110-611) and GFP-Nanobooster LOT 102 (1:500 ...
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bioRxiv - Immunology 2023Quote: ... then incubated for 15 min at 4 °C in labeling buffer containing streptavidin-conjugated and anti-FITC MicroBeads (Miltenyi Biotec). Cells were negatively selected using MACS LD columns per the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... CD14+ monocytes were isolated from PBMC from healthy subjects by positive selection withanti-CD14 magnetic labeling beads and an isolation column (Miltenyi Biotec). Monocytes were resuspended in RPMI 1640 medium (Gibco ...
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bioRxiv - Immunology 2020Quote: Total CD4+ T cells from baseline leukapheresis were isolated from cryopreserved PBMCs by manual magnetic labeling and negative selection using the CD4+ T Cell Isolation Kit (Miltenyi Biotec).
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bioRxiv - Clinical Trials 2019Quote: Total CD4+ T cells were isolated from cryopreserved PBMCs by manual magnetic labeling and negative selection using the CD4+ T Cell Isolation Kit (Miltenyi Biotec).
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bioRxiv - Immunology 2023Quote: ... Human CD8+ T cells were isolated from healthy donors via magnetic bead labeling (CD8+ T cell isolation kit, Miltenyi Biotec, Germany). U937 (ATCC ...
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bioRxiv - Cancer Biology 2019Quote: Cytotoxic CD8+ T cells were isolated from spleens of OT-I transgenic mice by magnetic labeling and separation (Miltenyi Biotec, Cambridge, MA). Briefly ...
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bioRxiv - Genomics 2024Quote: ... The resulting cells were enriched for microglia with magnetic labeling and positive selection using CD11b MicroBeads (Miltenyi Biotec cat. #130-093-634) and LS columns ...
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bioRxiv - Immunology 2020Quote: ... and the complete sequence of the nucleocapsid phosphoprotein N and the membrane glycoprotein M (GenBank MN908947.3, Protein QHD43416.1, Protein QHD43423.2, Protein QHD43419.1) (Miltenyi Biotec, Germany). After 5 hours of stimulation ...
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bioRxiv - Cancer Biology 2020Quote: ... Once counted CD14+ human monocytes were isolated from PBMCs using magnetic labeling (Monocyte Isolation Kit II, Cat. 130-091-153, Miltenyi Biotec, San Diego, CA) and magnetic columns as per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Once counted CD14+ human monocytes were isolated from PBMCs using magnetic labeling (Monocyte Isolation Kit II, Cat. 130-091-153, Miltenyi Biotec, San Diego, CA) and magnetic columns as per manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... CD45− cells were enriched by autoMACS separation after magnetic labeling of CD45+ cells using anti-CD45-APC followed by anti-APC microbeads (Miltenyi Biotec, Cat. #130-090-855) or anti-CD45 Nanobeads (Biolegend ...
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bioRxiv - Cell Biology 2024Quote: ... supernatant was removed and pellet was resuspended in CMF-B (uptake) or 1X PBS (EdU labeling) then passed through a 70 µm filter (Miltenyi Biotec MACS SmartStrainers, 130-098-462) to remove aggregates.
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bioRxiv - Plant Biology 2020Quote: ... Immunoprecipitation to isolate the protein complexes from total protein extracts using GFP-tagged microbeads (Miltenyi Biotec) has been described previously [8] ...
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bioRxiv - Cell Biology 2021Quote: ... Interacting proteins were isolated by applying total protein extracts to α-GFP-coupled magnetic beads (Miltenyi Biotec). Three replicates of TPLATE motif substitution mutants (SWM and PFM ...
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bioRxiv - Plant Biology 2019Quote: ... Interacting proteins were isolated from total protein extracts using anti-GFP-coupled magnetic beads (Miltenyi Biotec, Bergisch Gladbach, Germany). Proteins were digested with trypsin to peptides ...
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bioRxiv - Plant Biology 2020Quote: ... and proteins eluted with Elution Buffer (Miltenyi Biotec). Protein extracts were the subjected to immunoblot analyses using α-myc or α-HA antibodies.
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bioRxiv - Microbiology 2022Quote: ... MultiMACS protein A/G microbeads (Miltenyi Biotec, Germany) were used to catch the secreted proteins as described by[35].
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bioRxiv - Microbiology 2023Quote: ... The remaining supernatants were adjusted so that they had the same amount of total protein and mixed with 25 μL α-FLAG magnetic bead as instructed in the μMACS Epitope Tag Protein Isolation Kit (Miltenyi Biotec). From here ...
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bioRxiv - Immunology 2020Quote: ... and spike proteins (Prot_S) were purchased from Miltenyi Biotec. Optimal peptides for the manufacture of HLA class I tetramers were synthesized at >95% purity by Peptides & Elephants GmbH ...
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bioRxiv - Cell Biology 2019Quote: The μMACS GFP-tagged protein isolation kit (Miltenyi Biotec) was used to pull down proteins associated with the GFP-tagged protein ...
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bioRxiv - Immunology 2021Quote: ... M and N protein were purchased from Miltenyi Biotec. Domains of SARS-CoV-2 S protein showed in Figure S2A was previously described (Wrapp et al. ...
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bioRxiv - Plant Biology 2022Quote: ... Proteins were eluted with 100 μL elution buffer (Miltenyi Biotec). Proteins were resolved in 8% SDS-PAGE gels described above.
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bioRxiv - Immunology 2020Quote: Protein A beads and µ columns were from Miltenyi Biotec Ltd. ...
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bioRxiv - Molecular Biology 2019Quote: ... Co-immunoprecipitated proteins were isolated using magnetic μMACS columns (Miltenyi Biotec) as previously described61 ...
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bioRxiv - Microbiology 2022Quote: ... then 50 μL of protein G conjugated nanobeads (Miltenyi Biotec, Germany) were added on samples ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were eluted off the beads with elution buffer (Miltenyi Biotec). Proteins were resolved using SDS polyacrylamide gel electrophoresis and gels were sent to the Rutgers Biological Mass Spectrometry Facility ...
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bioRxiv - Plant Biology 2023Quote: ... Protein-DNA complexes were immunoprecipitated using anti-HA antibodies (Miltenyi Biotec) (Kaufmann et al. ...
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bioRxiv - Plant Biology 2019Quote: ... Proteins were detected with α-GFP-HRP (Miltenyi Biotec, 130-091-833), α-Myc-HRP (Abcam ...
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bioRxiv - Cell Biology 2022Quote: ... and 100 μL of μMACSTM Protein A/G MicroBeads (Miltenyi Biotec, Germany) were added for 1 hr incubation before applying to pre-equilibrated μ columns ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 spike PepTivator® protein pools (Miltenyi Biotec, Gladbach, GER) were used to test T cell responses against full spike proteome ...
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bioRxiv - Plant Biology 2019Quote: ... immuno-purification was performed with µMACS GFP Tag Protein Isolation kits (Miltenyi Biotec). 1 mg of solubilized microsomes were loaded on µcolumns ...
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bioRxiv - Plant Biology 2022Quote: ... μMACS™ GFP or μMACS™ DYKDDDDK (FLAG) Protein Isolation Kits (Miltenyi Biotec) were used for immunoprecipitations ...
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bioRxiv - Bioengineering 2021Quote: ... supplemented with 10 µM rho-associated protein kinase inhibitor Y-27632 (Miltenyi Biotec) for the first 24 h after passaging ...
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bioRxiv - Plant Biology 2021Quote: ... Proteins were immunoprecipitated by 50 µl Anti-HA MicroBeads (Miltenyi Biotec, Bergisch Gladbach, Germany) after incubation for 2.5 h at 4 °C while slowly rotating ...
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bioRxiv - Immunology 2021Quote: ... resuspended in 100 µL PBE with 5 μL μMACS Protein G MicroBeads (Miltenyi Biotec), and incubated with shaking for 30 minutes at 4 ⁰C ...
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bioRxiv - Immunology 2022Quote: ... or S-protein (B.1.351) mutation peptide pool (Miltenyi Biotec, # 130-127-958, Germany) at a concentration of 1.5 μg/ml/peptide at 37 °C for 16-18 h in pre-coated ELISpot plate (MSIPS4W10 from Millipore ...
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bioRxiv - Cell Biology 2021Quote: ... Antibodies were captured with 100µl of μMACS protein A beads (Miltenyi Biotec; San Diego, CA). Beads were isolated with μ columns and a μMACS separator (Miltenyi Biotec ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins were detected with horseradish peroxidase (HRP)-conjugated anti-GFP antibody (1:5,000; Miltenyi Biotec) for one hour at room temperature ...