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Citations for Miltenyi Biotec :
1 - 50 of 2979 citations for Primary Human Retinal Microvascular Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Human tumor cells were isolated from primary human omental metastases with human tumor cell isolation kit (Miltenyi Biotec, Cat# 130-108-339) using MACS® cell separation technology following the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Primary cells were blocked with human FcR blocking reagent (Miltenyi Biotec) and mouse-derived samples were additionally blocked with mouse FcR-blocking reagent (Miltenyi Biotec) ...
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bioRxiv - Immunology 2021Quote: ... Primary human CD8+ T cells were negatively isolated from PBMC using Human CD8+ T Cell isolation Kits (Miltenyi Biotec). Human CD8+ T cells were stimulated with CD3/CD28 activator beads (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Primary human CD4+ T cells were isolated by untouched CD4+ T cell isolation kit (Miltenyi Biotec). Human CD4+ T cells were activated by Dynabeads Human T-Activator CD3/CD28 (ThermoFisher ...
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bioRxiv - Bioengineering 2022Quote: Mature mouse and human retinal organoids were dissociated using the embryoid body dissociation kit (Miltenyi Biotec, 130-096-348) with the gentleMACS™ Dissociator according to the manufacturer’s automated dissociation protocol with a minor modification ...
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bioRxiv - Microbiology 2022Quote: ... Primary human T cells were purified by positive selection using CD3 MicroBeads (Miltenyi Biotec), as previously described (Goujon et al. ...
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bioRxiv - Synthetic Biology 2021Quote: ... Primary human T cells were purified from PBMCs by Pan T Cell Isolation Kit (Miltenyi Biotec, Germany). Purified T cells were stimulated with anti-CD3/CD28 Dynabeads (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... cryopreserved human PBMCs were thawed and primary human CD3+ T cells were isolated from PBMCs by using human Pan T Cell Isolation Kit (Miltenyi Biotec, 130-096-535). Cells were activated and expanded with Dynabeads at 1:1 ratio ...
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bioRxiv - Systems Biology 2019Quote: ... Endothelial cells were isolated using CD31-conjugated microbeads (Miltenyi Biotec). Initially ...
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bioRxiv - Developmental Biology 2021Quote: ... Endothelial cells were purified based on CD31 expression (Miltenyi Biotec Neonatal Cardiac Endothelial Cell Isolation Kit ...
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bioRxiv - Bioengineering 2024Quote: ... chambers were obtained from the Stanford Blood Center and primary human B cells were isolated by negative selection using the human B Cell Isolation Kit II (Miltenyi Biotec, cat: 130-091-151). Cells were cultured in Iscove’s modified Dulbecco’s medium (IMDM ...
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bioRxiv - Physiology 2023Quote: ... Endothelial cell isolation kit for rat (Miltenyi Biotec Inc., Auburn, CA) was used to isolate endothelial cells from this cell suspension following manufacturer protocol ...
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bioRxiv - Microbiology 2020Quote: Human primary CD4+ T cells are obtained from peripheral blood with CD4 T cell isolation kit II (Miltenyi Biotec). Unactivated CD4+ T cells were maintained in complete RPMI medium containing 50 U/ml interleukin 2 (IL-2 ...
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bioRxiv - Cancer Biology 2023Quote: Primary human inv(16) AML CD34+ cells were T-cell depleted using CD3 Microbeads (Miltenyi Biotec, #130-050-101) and nucleofected with the RNP complex ...
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bioRxiv - Microbiology 2023Quote: ... Enrichment of primary CD4+ T cells was performed using the EasySep™ Human CD4+ T Cell Enrichment Kit (Miltenyi Biotec) according to the manufacturer’s manual ...
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bioRxiv - Bioengineering 2021Quote: The GFP-positive cells in the retinal explant cell suspensions were enriched using a MACS Tyto sorter (Miltenyi Biotec). GFP-positive cells were resuspended in D-PBS with 0.1% BSA and processed immediately for scRNA-seq.
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bioRxiv - Developmental Biology 2022Quote: ... After enrichment for CD34+ endothelial progenitor cells (CD34 MicroBead Kit, Miltenyi Biotec), cells were expanded for one week in a T75 flask ...
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bioRxiv - Molecular Biology 2022Quote: Primary human T regulatory cells (Tregs) were isolated from PBMCs using CD4+ CD25+ CD127dim/- Regulatory T Cell Isolation Kit (Miltenyi Biotec). The isolated Treg cells were expanded in culture for 14 days with TexMACS medium (Miltenyi Biotec ...
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bioRxiv - Molecular Biology 2020Quote: ... patient cells were stained with the following primary antibodies: mouse anti–human CD3 (clone BW264/56; Miltenyi Biotec), mouse anti–human CD7 (clone M-T701 ...
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bioRxiv - Pathology 2023Quote: Endothelial cells were isolated from single cell suspension using LS Columns (#130-042-401, Miltenyi Biotec) and MACs magnetic beads with negative selection of CD45 (#130-052-301 ...
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bioRxiv - Physiology 2022Quote: ... the purity of the endothelial cells was determined using CD31 MicroBeads (Miltenyi Biotec). Briefly ...
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bioRxiv - Immunology 2022Quote: ... CD45+ cells (immune cells) and CD31+ (endothelial cells) were then depleted using mouse CD45 and CD31 MicroBeads (Miltenyi Biotec) and the remaining cells were counted and incubated with anti-CD326-APC (eBioscience) ...
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bioRxiv - Molecular Biology 2021Quote: ... endothelial (CD31+) and myeloid (CD45+) cells were sorted using the Magnetic Cell Isolation and Cell Separation kit (Miltenyi Biotec) with recommended dilutions of antibodies against CD31 (Miltenyi Biotec 130-097-418 ...
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bioRxiv - Cancer Biology 2021Quote: ... Primary human T cells were isolated from PBMCs by negative selection using the Pan T Isolation Kit (Miltenyi Biotec). Batch 1 T cells were cultured in IMDM Medium containing Icove’s Modified Dulbecco’s Medium (IMDM ...
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bioRxiv - Cell Biology 2023Quote: ... endothelial cells were purified using a CD31 MicroBead Kit (Miltenyi Biotec, 130-091-935) and further cultured in the Promocell endothelial cell medium (MV2 ...
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bioRxiv - Genetics 2024Quote: ... The PBMCs were further processed to isolate human primary T cells using MACS based PAN-T isolation kits (Miltenyi Biotec). RPMI-1640 (Gibco ...
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bioRxiv - Cell Biology 2021Quote: Isolation of mice lung endothelial cells was performed using gentle MACS Dissociator (Miltenyi Biotec Inc.), as indicated by the manufacturer ...
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bioRxiv - Cell Biology 2021Quote: ... Endothelial cells were isolated by using CD31+ MicroBeads (Miltenyi Biotec, Order no.130-091-935) according to manufacturer’ s instructions and cultured in EGM2 with 16% FBS (HyClone).
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A framework for defining mesenchymal cell types associated with murine periosteal and endosteal bonebioRxiv - Genomics 2023Quote: ... cells were depleted of hematopoietic cells and endothelial cells using a lineage depletion kit (Miltenyi Biotec, cat. no. 130-090-858) followed by CD45 ...
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bioRxiv - Microbiology 2022Quote: ... Human primary monocytes were isolated using MACS negative selection (Miltenyi Biotec, Cambridge, MA) from peripheral blood mononuclear cells obtained from healthy human donors (de-identified ...
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bioRxiv - Microbiology 2021Quote: ... 1x105 HEK-293T cells were mixed at a 1:1 ratio in 96-well plates with primary CD4+ T lymphocytes freshly isolated from healthy donors by negative selection (CD4+ T-Cell Isolation Kit II, human, Miltenyi Biotec). Viral transfer was assessed after 24 hours of incubation at 37°C in permeabilized (FIX & PERM Cell Permeabilization kit ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were dissociated using TrypLE Express and enriched for CD34+ endothelial progenitor cells using the CD34 MicroBead Kit (Miltenyi Biotec). Purified cells resuspended in EGM-2 media (Lonza ...
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bioRxiv - Neuroscience 2021Quote: Endothelial cells were enriched by depletion of CD45+ cells with CD45 microbeads (#130-052-301, Miltenyi Biotec, Auburn, CA, USA) followed by positive selection using CD31 microbeads (#130-097-418 ...
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bioRxiv - Neuroscience 2023Quote: Retinal tissues were dissociated using the Neural Tissue Dissociation Kit (P) (Miltenyi Biotec). Briefly ...
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bioRxiv - Microbiology 2021Quote: Primary human neutrophils were isolated via negative depletion method from human blood with MACSxpress® Whole Blood Neutrophil Isolation Kit (Miltenyi Biotec), following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Endothelial cells were enriched from PFC punches by using MACS according to the manufacturer’s protocol (Miltenyi Biotec). Briefly ...
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bioRxiv - Developmental Biology 2023Quote: ... endothelial cells (ECs) were isolated by incubation with CD31-coated magnetic microbeads (130-097-418, Miltenyi Biotec) and the positive selection was performed using a magnetic MACs separator ...
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bioRxiv - Cell Biology 2020Quote: ... Total proximal or distal dissociated cells were passed through a series of cell strainers of decreasing pore sizes from 500μm to 40μm under vacuum pressure and depleted of immune and endothelial cells by magnetic associated cell sorting (MACS) in accordance to the manufacturing protocol (Miltenyi Biotec). Viable epithelial cells were further enriched by fluorescence associated cell sorting (FACS ...
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bioRxiv - Molecular Biology 2024Quote: ... The cell suspension was sorted for CD31+ endothelial cells using anti-CD31 MicroBeads (Miltenyi Biotec, 130-097-418; San Diego, CA) and a magnetic cell isolation system (Miltenyi Biotec ...
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bioRxiv - Microbiology 2023Quote: ... Peripheral blood mononuclear cells were obtained from deidentified healthy human donors (Gulf Coast Regional Blood Center, Houston, TX) and primary human monocytes isolated using MACS negative selection (Miltenyi Biotec, Cambridge, MA). E ...
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bioRxiv - Immunology 2024Quote: ... Human T cells were enriched from PBMCs using the EasySep Negative Human T Cell kit (Miltenyi Biotec) according to manufacturer instructions and labeled with 5 M Tag-it Violet (Biolegend ...
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bioRxiv - Synthetic Biology 2023Quote: Functional viral titres of retroviral supernatant were calculated using frozen supernatant on primary human T cells activated with TransAct (Miltenyi Biotec; 130-111-160), 10 ng/ml IL-7 (Miltenyi Biotec ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Human B cells were isolated from PBMC collected from human volunteer donors by magnetic-activated cell sorting using the B cell Isolation Kit II for human cells from Miltenyi Biotec Inc (Auburn ...
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bioRxiv - Cell Biology 2023Quote: ... human CD45+ cells were purified from the remaining fresh murine BM cells using human CD45 MicroBeads (Miltenyi Biotec), and subsequently transplanted into twelve- to thirteen-week-old low-dose busulfan conditioned NBSGW female mice ...
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bioRxiv - Cell Biology 2022Quote: ... After an additional 24h co-cultured cells were trypsinized and endothelial cells were purified using CD31 microbead kit (Miltenyi Biotec #130-091-935) according to manufacturer instructions.
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bioRxiv - Immunology 2024Quote: Primary T cells were activated with T Cell TransAct (Miltenyi Biotec) and 48 hours later co-transduced by a mixture of CAR19 LVs and LOP18 LVs ...
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bioRxiv - Systems Biology 2023Quote: Isolation of endothelial cells and fibroblasts from adult mouse hearts was performed with MACS-magnetic beads from Miltenyi Biotec based on the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... followed by the separation of neurons from non-neuronal cells (astrocytes, oligodendrocytes, microglia, endothelial cells, and fibroblasts) using the Neuron Isolation Kit (Miltenyi Biotec, 130-115-389) following the kitś instructions ...
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bioRxiv - Biophysics 2023Quote: ... Human primary classical monocytes (CD14+CD16-) were further isolated using the Classical Monocyte Isolation Kit (Miltenyi Biotec) by magnetic-activated cell sorting (MACS) ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells were purified from human PBMCs by using human CD4 MicroBeads (Miltenyi Biotec). CD4+ CD25Lo CD127Hi Tconv cells and CD4+ CD25Hi CD127Lo Treg cells were sorted by flow cytometry and stimulated in 96-well cell-culture plates with three times the number of Dynabeads M-450 Tosylactivated beads (Thermo Fisher Scientific ...