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Citations for Miltenyi Biotec :
1 - 50 of 2976 citations for Primary Human Melanocyte Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Human tumor cells were isolated from primary human omental metastases with human tumor cell isolation kit (Miltenyi Biotec, Cat# 130-108-339) using MACS® cell separation technology following the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Primary cells were blocked with human FcR blocking reagent (Miltenyi Biotec) and mouse-derived samples were additionally blocked with mouse FcR-blocking reagent (Miltenyi Biotec) ...
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bioRxiv - Immunology 2021Quote: ... Primary human CD8+ T cells were negatively isolated from PBMC using Human CD8+ T Cell isolation Kits (Miltenyi Biotec). Human CD8+ T cells were stimulated with CD3/CD28 activator beads (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Primary human CD4+ T cells were isolated by untouched CD4+ T cell isolation kit (Miltenyi Biotec). Human CD4+ T cells were activated by Dynabeads Human T-Activator CD3/CD28 (ThermoFisher ...
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bioRxiv - Microbiology 2022Quote: ... Primary human T cells were purified by positive selection using CD3 MicroBeads (Miltenyi Biotec), as previously described (Goujon et al. ...
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bioRxiv - Synthetic Biology 2021Quote: ... Primary human T cells were purified from PBMCs by Pan T Cell Isolation Kit (Miltenyi Biotec, Germany). Purified T cells were stimulated with anti-CD3/CD28 Dynabeads (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... cryopreserved human PBMCs were thawed and primary human CD3+ T cells were isolated from PBMCs by using human Pan T Cell Isolation Kit (Miltenyi Biotec, 130-096-535). Cells were activated and expanded with Dynabeads at 1:1 ratio ...
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bioRxiv - Bioengineering 2024Quote: ... chambers were obtained from the Stanford Blood Center and primary human B cells were isolated by negative selection using the human B Cell Isolation Kit II (Miltenyi Biotec, cat: 130-091-151). Cells were cultured in Iscove’s modified Dulbecco’s medium (IMDM ...
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bioRxiv - Microbiology 2020Quote: Human primary CD4+ T cells are obtained from peripheral blood with CD4 T cell isolation kit II (Miltenyi Biotec). Unactivated CD4+ T cells were maintained in complete RPMI medium containing 50 U/ml interleukin 2 (IL-2 ...
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bioRxiv - Cancer Biology 2023Quote: Primary human inv(16) AML CD34+ cells were T-cell depleted using CD3 Microbeads (Miltenyi Biotec, #130-050-101) and nucleofected with the RNP complex ...
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bioRxiv - Microbiology 2023Quote: ... Enrichment of primary CD4+ T cells was performed using the EasySep™ Human CD4+ T Cell Enrichment Kit (Miltenyi Biotec) according to the manufacturer’s manual ...
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bioRxiv - Molecular Biology 2022Quote: Primary human T regulatory cells (Tregs) were isolated from PBMCs using CD4+ CD25+ CD127dim/- Regulatory T Cell Isolation Kit (Miltenyi Biotec). The isolated Treg cells were expanded in culture for 14 days with TexMACS medium (Miltenyi Biotec ...
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bioRxiv - Molecular Biology 2020Quote: ... patient cells were stained with the following primary antibodies: mouse anti–human CD3 (clone BW264/56; Miltenyi Biotec), mouse anti–human CD7 (clone M-T701 ...
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bioRxiv - Cancer Biology 2021Quote: ... Primary human T cells were isolated from PBMCs by negative selection using the Pan T Isolation Kit (Miltenyi Biotec). Batch 1 T cells were cultured in IMDM Medium containing Icove’s Modified Dulbecco’s Medium (IMDM ...
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bioRxiv - Genetics 2024Quote: ... The PBMCs were further processed to isolate human primary T cells using MACS based PAN-T isolation kits (Miltenyi Biotec). RPMI-1640 (Gibco ...
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bioRxiv - Microbiology 2022Quote: ... Human primary monocytes were isolated using MACS negative selection (Miltenyi Biotec, Cambridge, MA) from peripheral blood mononuclear cells obtained from healthy human donors (de-identified ...
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bioRxiv - Microbiology 2021Quote: ... 1x105 HEK-293T cells were mixed at a 1:1 ratio in 96-well plates with primary CD4+ T lymphocytes freshly isolated from healthy donors by negative selection (CD4+ T-Cell Isolation Kit II, human, Miltenyi Biotec). Viral transfer was assessed after 24 hours of incubation at 37°C in permeabilized (FIX & PERM Cell Permeabilization kit ...
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bioRxiv - Microbiology 2021Quote: Primary human neutrophils were isolated via negative depletion method from human blood with MACSxpress® Whole Blood Neutrophil Isolation Kit (Miltenyi Biotec), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Peripheral blood mononuclear cells were obtained from deidentified healthy human donors (Gulf Coast Regional Blood Center, Houston, TX) and primary human monocytes isolated using MACS negative selection (Miltenyi Biotec, Cambridge, MA). E ...
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bioRxiv - Immunology 2024Quote: ... Human T cells were enriched from PBMCs using the EasySep Negative Human T Cell kit (Miltenyi Biotec) according to manufacturer instructions and labeled with 5 M Tag-it Violet (Biolegend ...
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bioRxiv - Synthetic Biology 2023Quote: Functional viral titres of retroviral supernatant were calculated using frozen supernatant on primary human T cells activated with TransAct (Miltenyi Biotec; 130-111-160), 10 ng/ml IL-7 (Miltenyi Biotec ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Human B cells were isolated from PBMC collected from human volunteer donors by magnetic-activated cell sorting using the B cell Isolation Kit II for human cells from Miltenyi Biotec Inc (Auburn ...
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bioRxiv - Cell Biology 2023Quote: ... human CD45+ cells were purified from the remaining fresh murine BM cells using human CD45 MicroBeads (Miltenyi Biotec), and subsequently transplanted into twelve- to thirteen-week-old low-dose busulfan conditioned NBSGW female mice ...
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bioRxiv - Immunology 2024Quote: Primary T cells were activated with T Cell TransAct (Miltenyi Biotec) and 48 hours later co-transduced by a mixture of CAR19 LVs and LOP18 LVs ...
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bioRxiv - Biophysics 2023Quote: ... Human primary classical monocytes (CD14+CD16-) were further isolated using the Classical Monocyte Isolation Kit (Miltenyi Biotec) by magnetic-activated cell sorting (MACS) ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells were purified from human PBMCs by using human CD4 MicroBeads (Miltenyi Biotec). CD4+ CD25Lo CD127Hi Tconv cells and CD4+ CD25Hi CD127Lo Treg cells were sorted by flow cytometry and stimulated in 96-well cell-culture plates with three times the number of Dynabeads M-450 Tosylactivated beads (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... and T Cell TransAct™ human (Miltenyi Biotec) for 72 h.
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bioRxiv - Synthetic Biology 2020Quote: ... PBMC were isolated from a Buffy Coat from healthy volunteer donors using Ficoll gradient centrifugation and human T cells were isolated using the Human Pan T cell isolation kit (Miltenyi Biotec). Human T cells were cultured in supplemented RPMI media as described for cell lines above ...
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bioRxiv - Immunology 2021Quote: ... and human CD45+ cells were selected by magnetic cell sorting (Miltenyi Biotec). scRNAseq libraries were prepared with the 10x Genomics’ Chromium Single Cell 3’ Library & Gel Bead Kit v2 ...
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bioRxiv - Immunology 2022Quote: ... and NK cells using the human NK Cell Isolation Kit (Miltenyi Biotec). The viability for each cell type was determined using a micro flow cytometer with propidium iodide (Moxi Flow ...
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bioRxiv - Systems Biology 2019Quote: ... Human cells expressing CD45 (Miltenyi Biotec, 130-045-801) or CD235a (Miltenyi Biotec ...
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bioRxiv - Immunology 2019Quote: ... CD56+ NK cells (mouse anti-human CD56, Miltenyi Biotec) and CD19+ B cells (mouse anti-human CD19 ...
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bioRxiv - Immunology 2021Quote: ... or Human CD8+ T Cell Isolation Kit (Miltenyi Biotec), stimulated with Dynabeads™ Human T-Activator CD3/CD28 (ThermoFisher Scientific ...
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bioRxiv - Immunology 2020Quote: Primary human CD14+ monocytes were positively selected from PBMCs using CD14 Microbeads according to the manufacturer’s instructions (Miltenyi Biotec) and differentiated for 3 days with 20 ng/mL M-CSF ...
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bioRxiv - Synthetic Biology 2023Quote: ... T cells were activated with TransAct Human T cell activation reagent (Miltenyi Biotec) and 100 U/ml human IL-2 IS (Miltenyi Biotec) ...
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bioRxiv - Microbiology 2021Quote: ... Primary T-cells were purified using the Pan T-cell Isolation Kit (Miltenyi Biotec). HLA-DP4 restricted WT1 specific TCR (clone 9)(27 ...
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bioRxiv - Genetics 2021Quote: ... CD14+ monocytes and CD19+ B cells were isolated from human PBMCs using the Human CD3+ T Cell Isolation kit (Miltenyi Biotec,130-050-101), Human CD14+ monocytes Isolation kit (Miltenyi Biotec ...
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bioRxiv - Genetics 2022Quote: ... Then cells were incubated with human CD34 Microbeads (Miltenyi Biotec) and Fc receptor blocker in MACS buffer (1% BSA/2mM EDTA/PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... Human T Cell TransAct (Miltenyi Biotec t# 130-111-160) was used to activate T cells ...
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bioRxiv - Biochemistry 2023Quote: Human primary monocytes were isolated from healthy donors using the autoMACS Pan Monocyte Isolation Kit (Miltenyi Biotec, 130-096-537). Briefly ...
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bioRxiv - Immunology 2021Quote: ... T cells were isolated using the human Pan T cell Isolation Kit (Miltenyi Biotec) and naive CD4+ T cell using the Naive CD4+ T Cell Isolation (Miltenyi Biotec) ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were washed in complete media and CD45+ cells isolated using CD45 magnetic bead separation for non-human primates (Miltenyi Biotec CD45 Microbeads non-human primate) in accordance with the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were washed in complete media and CD45+ cells isolated using CD45 magnetic bead separation for non-human primates (Miltenyi Biotec CD45 Microbeads non-human primate) in accordance with the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... resting NK cells were obtained using the human NK cell isolation kit (MACS Miltenyi Biotec), following manufacture’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... and CD4+ T cells were isolated using human CD4+T cell isolation kit (Miltenyi Biotec). Purified CD4+T cells were washed and stained with 3 µM CFSE (Invitrogen) ...
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bioRxiv - Synthetic Biology 2020Quote: ... T cells were stimulated and expanded using TransAct Human T cell activation reagent (Miltenyi Biotec). For transduction ...
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bioRxiv - Immunology 2022Quote: ... T cells were purified using the human Naïve Pan T Cell Isolation Kit (Miltenyi Biotec) and NK cells using the human NK Cell Isolation Kit (Miltenyi Biotec) ...
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bioRxiv - Immunology 2022Quote: ... using the human CD4+ T cell isolation kit (MACS Miltenyi Biotec). Isolated T helper cell purity was checked by flow cytometry CD4 staining and they were then cultured in CD3 (5µg/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were suspended in human FC block (Miltenyi Biotec Inc., FLP3330) and kept on ice for 30 minutes ...
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bioRxiv - Bioengineering 2024Quote: ... were stimulated with TransAct Human T cell activation reagent (Miltenyi Biotec), 100 U/ml human IL-2 IS (Miltenyi Biotec ...