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Citations for Miltenyi Biotec :
1 - 50 of 2996 citations for Primary Human Cardiac Fibroblast Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2022Quote: Primary cardiac fibroblasts were isolated from 2-day-old rats using a neonatal heart dissociation kit (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... One quarter of the of the cell suspension volume human anti-fibroblast Microbeads (Miltenyi Biotec) were added for 30 min at RT ...
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bioRxiv - Cancer Biology 2023Quote: ... Human tumor cells were isolated from primary human omental metastases with human tumor cell isolation kit (Miltenyi Biotec, Cat# 130-108-339) using MACS® cell separation technology following the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Primary cells were blocked with human FcR blocking reagent (Miltenyi Biotec) and mouse-derived samples were additionally blocked with mouse FcR-blocking reagent (Miltenyi Biotec) ...
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bioRxiv - Bioengineering 2023Quote: ... Conjugated primary antibodies used was anti-cardiac troponin 2 (TNNT2) PE (Miltenyi Biotec – 130-120-405). Viability marker Zombie Aqua (Biolegend – 423102 ...
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bioRxiv - Immunology 2021Quote: ... Primary human CD8+ T cells were negatively isolated from PBMC using Human CD8+ T Cell isolation Kits (Miltenyi Biotec). Human CD8+ T cells were stimulated with CD3/CD28 activator beads (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2023Quote: For primary cardiac myofibroblast enriched cultures cells were first isolated from adult mice hearts using a neonatal dissociation kit (Miltenyi Biotec,130-098-373) and gentleMACS homogenizer (Miltenyi Biotec) ...
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bioRxiv - Molecular Biology 2020Quote: Primary cardiac cultures were isolated from P7 mice using a neonatal dissociation kit (Miltenyi Biotec,130-098-373) using the gentleMACS homogenizer ...
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bioRxiv - Cell Biology 2020Quote: ... Primary human CD4+ T cells were isolated by untouched CD4+ T cell isolation kit (Miltenyi Biotec). Human CD4+ T cells were activated by Dynabeads Human T-Activator CD3/CD28 (ThermoFisher ...
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bioRxiv - Developmental Biology 2020Quote: Neonatal primary cardiac cultures were isolated from P0 pups with a neonatal dissociation kit (Miltenyi Biotec, 130-098-373) and homogenizer (gentleMACS) ...
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bioRxiv - Microbiology 2022Quote: ... Primary human T cells were purified by positive selection using CD3 MicroBeads (Miltenyi Biotec), as previously described (Goujon et al. ...
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bioRxiv - Synthetic Biology 2021Quote: ... Primary human T cells were purified from PBMCs by Pan T Cell Isolation Kit (Miltenyi Biotec, Germany). Purified T cells were stimulated with anti-CD3/CD28 Dynabeads (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... cryopreserved human PBMCs were thawed and primary human CD3+ T cells were isolated from PBMCs by using human Pan T Cell Isolation Kit (Miltenyi Biotec, 130-096-535). Cells were activated and expanded with Dynabeads at 1:1 ratio ...
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bioRxiv - Bioengineering 2024Quote: ... chambers were obtained from the Stanford Blood Center and primary human B cells were isolated by negative selection using the human B Cell Isolation Kit II (Miltenyi Biotec, cat: 130-091-151). Cells were cultured in Iscove’s modified Dulbecco’s medium (IMDM ...
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bioRxiv - Microbiology 2020Quote: Human primary CD4+ T cells are obtained from peripheral blood with CD4 T cell isolation kit II (Miltenyi Biotec). Unactivated CD4+ T cells were maintained in complete RPMI medium containing 50 U/ml interleukin 2 (IL-2 ...
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bioRxiv - Cancer Biology 2023Quote: Primary human inv(16) AML CD34+ cells were T-cell depleted using CD3 Microbeads (Miltenyi Biotec, #130-050-101) and nucleofected with the RNP complex ...
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bioRxiv - Bioengineering 2022Quote: ... for the cardiac marker Cardiac troponin T conjugated to APC (cTnT; 1:50; REA400; 130-120-543, Miltenyi Biotec, Germany), and cells were frozen in RPMI + B27C with 10 µM of RI + 10% FCS + 10% DMSO ...
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bioRxiv - Bioengineering 2020Quote: ... Cardiac cells were isolated by excluding dead cells using the MACS dead cell removal kit (Miltenyi Biotec, 130-090-101) and were suspended in the optimized culture medium for cardiac cell culture ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were incubated with cardiac troponin T APC-conjugated antibody (Miltenyi Biotec, 130-120-403) or REA control human IgG1 APC-conjugated isotype antibody (Miltenyi Biotec ...
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bioRxiv - Microbiology 2023Quote: ... Enrichment of primary CD4+ T cells was performed using the EasySep™ Human CD4+ T Cell Enrichment Kit (Miltenyi Biotec) according to the manufacturer’s manual ...
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bioRxiv - Genomics 2019Quote: ... the streptavidin purification step served to collect the thiolated-RNA fraction (cardiac-specific 4TU) and the unbound fraction (non-cardiac RNAs) using a µMACS Streptavidin kit (MACS, Miltenyi Biotec) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: Primary human T regulatory cells (Tregs) were isolated from PBMCs using CD4+ CD25+ CD127dim/- Regulatory T Cell Isolation Kit (Miltenyi Biotec). The isolated Treg cells were expanded in culture for 14 days with TexMACS medium (Miltenyi Biotec ...
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bioRxiv - Molecular Biology 2020Quote: ... patient cells were stained with the following primary antibodies: mouse anti–human CD3 (clone BW264/56; Miltenyi Biotec), mouse anti–human CD7 (clone M-T701 ...
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bioRxiv - Cancer Biology 2021Quote: ... Primary human T cells were isolated from PBMCs by negative selection using the Pan T Isolation Kit (Miltenyi Biotec). Batch 1 T cells were cultured in IMDM Medium containing Icove’s Modified Dulbecco’s Medium (IMDM ...
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bioRxiv - Bioengineering 2020Quote: ... Neonatal cardiac EC isolation kit (Miltenyi Biotec., 130-104-183) was used to further isolate cardiac ECs from the primary cardiac cell population [68] ...
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bioRxiv - Genetics 2024Quote: ... The PBMCs were further processed to isolate human primary T cells using MACS based PAN-T isolation kits (Miltenyi Biotec). RPMI-1640 (Gibco ...
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bioRxiv - Microbiology 2022Quote: ... Human primary monocytes were isolated using MACS negative selection (Miltenyi Biotec, Cambridge, MA) from peripheral blood mononuclear cells obtained from healthy human donors (de-identified ...
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bioRxiv - Microbiology 2021Quote: ... 1x105 HEK-293T cells were mixed at a 1:1 ratio in 96-well plates with primary CD4+ T lymphocytes freshly isolated from healthy donors by negative selection (CD4+ T-Cell Isolation Kit II, human, Miltenyi Biotec). Viral transfer was assessed after 24 hours of incubation at 37°C in permeabilized (FIX & PERM Cell Permeabilization kit ...
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bioRxiv - Cell Biology 2020Quote: ... On day 15 of cardiac differentiation fixed cells were labeled for flow cytometry using alpha-actinin (Miltenyi Biotec, Cat.#130106937) and corresponding isotype control ...
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bioRxiv - Microbiology 2021Quote: Primary human neutrophils were isolated via negative depletion method from human blood with MACSxpress® Whole Blood Neutrophil Isolation Kit (Miltenyi Biotec), following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... and anti-cardiac troponin-T-APC (Miltenyi Biotec, 130-120-403, 1:50) in blocking buffer (PBS ...
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bioRxiv - Microbiology 2023Quote: ... Peripheral blood mononuclear cells were obtained from deidentified healthy human donors (Gulf Coast Regional Blood Center, Houston, TX) and primary human monocytes isolated using MACS negative selection (Miltenyi Biotec, Cambridge, MA). E ...
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bioRxiv - Immunology 2024Quote: ... Human T cells were enriched from PBMCs using the EasySep Negative Human T Cell kit (Miltenyi Biotec) according to manufacturer instructions and labeled with 5 M Tag-it Violet (Biolegend ...
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bioRxiv - Synthetic Biology 2023Quote: Functional viral titres of retroviral supernatant were calculated using frozen supernatant on primary human T cells activated with TransAct (Miltenyi Biotec; 130-111-160), 10 ng/ml IL-7 (Miltenyi Biotec ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Human B cells were isolated from PBMC collected from human volunteer donors by magnetic-activated cell sorting using the B cell Isolation Kit II for human cells from Miltenyi Biotec Inc (Auburn ...
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bioRxiv - Cell Biology 2023Quote: ... human CD45+ cells were purified from the remaining fresh murine BM cells using human CD45 MicroBeads (Miltenyi Biotec), and subsequently transplanted into twelve- to thirteen-week-old low-dose busulfan conditioned NBSGW female mice ...
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bioRxiv - Cancer Biology 2021Quote: After separation with Anti-Fibroblast MicroBeads (Miltenyi Biotec), the sorted cell compartments and monocultures were counted and aliquots of 1×106 cells were prepared per condition ...
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bioRxiv - Cancer Biology 2021Quote: After separation with Anti-Fibroblast MicroBeads (Miltenyi Biotec), the sorted cell compartments and monocultures were counted and aliquots of 5×105 cells were prepared per condition ...
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bioRxiv - Cell Biology 2020Quote: Neonatal mouse cardiac myocytes (NMCMs) were isolated using the MACS cell separation kit (Miltenyi Biotec: Catalog #130-100-825 and #130-098-373). Briefly ...
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bioRxiv - Immunology 2024Quote: Primary T cells were activated with T Cell TransAct (Miltenyi Biotec) and 48 hours later co-transduced by a mixture of CAR19 LVs and LOP18 LVs ...
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bioRxiv - Cancer Biology 2023Quote: ... Tumor cells were separated from the fibroblast population using CD326 (EpCAM) MicroBeads (Miltenyi Biotec, Bergisch Gladbach, Germany) via magnetic-activated cell sorting (MACS ...
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bioRxiv - Biophysics 2023Quote: ... Human primary classical monocytes (CD14+CD16-) were further isolated using the Classical Monocyte Isolation Kit (Miltenyi Biotec) by magnetic-activated cell sorting (MACS) ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells were purified from human PBMCs by using human CD4 MicroBeads (Miltenyi Biotec). CD4+ CD25Lo CD127Hi Tconv cells and CD4+ CD25Hi CD127Lo Treg cells were sorted by flow cytometry and stimulated in 96-well cell-culture plates with three times the number of Dynabeads M-450 Tosylactivated beads (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... and T Cell TransAct™ human (Miltenyi Biotec) for 72 h.
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bioRxiv - Synthetic Biology 2020Quote: ... PBMC were isolated from a Buffy Coat from healthy volunteer donors using Ficoll gradient centrifugation and human T cells were isolated using the Human Pan T cell isolation kit (Miltenyi Biotec). Human T cells were cultured in supplemented RPMI media as described for cell lines above ...
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bioRxiv - Immunology 2021Quote: ... and human CD45+ cells were selected by magnetic cell sorting (Miltenyi Biotec). scRNAseq libraries were prepared with the 10x Genomics’ Chromium Single Cell 3’ Library & Gel Bead Kit v2 ...
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bioRxiv - Immunology 2022Quote: ... and NK cells using the human NK Cell Isolation Kit (Miltenyi Biotec). The viability for each cell type was determined using a micro flow cytometer with propidium iodide (Moxi Flow ...
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bioRxiv - Systems Biology 2023Quote: Isolation of endothelial cells and fibroblasts from adult mouse hearts was performed with MACS-magnetic beads from Miltenyi Biotec based on the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... followed by the separation of neurons from non-neuronal cells (astrocytes, oligodendrocytes, microglia, endothelial cells, and fibroblasts) using the Neuron Isolation Kit (Miltenyi Biotec, 130-115-389) following the kitś instructions ...
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bioRxiv - Systems Biology 2019Quote: ... Human cells expressing CD45 (Miltenyi Biotec, 130-045-801) or CD235a (Miltenyi Biotec ...