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Citations for Miltenyi Biotec :
1 - 50 of 2622 citations for Peripheral Blood Mononuclear Cell since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Human peripheral blood mononuclear cells (PBMC) were purified from whole blood by density gradient centrifugation (FicollPaqueTM, Miltenyi Biotec). Whole blood was collected from healthy donors through an NIH Department of Transfusion Medicine (DTM)-approved protocol (Institutional Review Board of the National Institute of Allergy and Infectious Diseases [NIAID]) ...
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bioRxiv - Immunology 2020Quote: CD14pos cells were isolated from peripheral blood mononuclear cells (PBMC) using CD14 micro-beads (Miltenyi Biotec) with the autoMACS Pro Separator according to the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Donor peripheral blood mononuclear cells (PBMCs) were isolated from leukopaks with a PBMC isolation kit (Miltenyi Biotec). PBMCs were activated with ImmunoCult human CD3/CD28 T cell activator (STEMCELL Technologies ...
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bioRxiv - Immunology 2022Quote: ... density gradient centrifugation to isolate peripheral blood mononuclear cells (PBMC) and subsequently using CD4 magnetic MicroBeads (Miltenyi Biotec) according to the manufacturers’ protocols ...
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bioRxiv - Immunology 2024Quote: ... Primary human CD4+ T cells were isolated from peripheral blood mononuclear cells obtained from healthy donors using Human CD4+ T Cell Isolation Kit (Miltenyi Biotec) according to the manual ...
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bioRxiv - Immunology 2020Quote: ... Peripheral blood mononuclear cells (PBMCs) from healthy donors (HD) were used to isolate monocytes (using CD14+ microbeads, Miltenyi Biotec) and to sort CD1c+ DC and slanDCs using FACS (Aria IIIu ...
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bioRxiv - Immunology 2020Quote: ... human peripheral blood mononuclear cells were activated with 50□ng/mL OKT3 (Cat. No. 130-093-387, Miltenyi Biotec) and harvested for retroviral transduction on day 2 ...
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bioRxiv - Immunology 2022Quote: Tregs were isolated from peripheral blood mononuclear cells (PBMCs) by negative selection of CD4 (Miltenyi Biotec, Bergisch Gladbach, Germany) followed by positive CD25 selection (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: ... Human peripheral blood mononuclear cells purchased from STEMCELL (70025) was stimulated by OKT-3 (Miltenyi Biotec, 130-093-387) and CD28 (BD Pharmingen ...
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bioRxiv - Immunology 2020Quote: ... CD14+ monocytes were isolated from peripheral blood mononuclear cells (PBMC) using magnetic bead selection (Miltenyi Biotec, San Diego, CA, USA). To differentiate cells into macrophages ...
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bioRxiv - Immunology 2019Quote: Human monocytes were purified from peripheral blood mononuclear cells (PBMCs) from healthy donors by positive immunoselection with anti-CD14-conjugated MACS beads (Miltenyi Biotec). Monocytes were also purified from PBMCs from either HLA-A2 WT NOD2 donors or HLA-A2 homozygous mutant NOD2 Crohn’s patients ...
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bioRxiv - Immunology 2019Quote: Human monocytes were purified from healthy donor peripheral blood mononuclear cells (PBMCs) by positive immunoselection with anti-CD14-conjugated MACS beads (Miltenyi Biotec). moDCs were obtained by culturing monocytes for 5 days with IL-4 and GM-CSF (Peprotech) ...
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bioRxiv - Microbiology 2020Quote: Primary human CD14+ monocytes were isolated from the peripheral blood mononuclear cells of healthy donors using anti-CD14 magnetic beads (Miltenyi Biotec) per the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... through peripheral blood mononuclear cell isolation by Ficoll density gradient centrifugation and magnetic separation using CD14 MicroBeads (130-050-201, Miltenyi Biotec), according to manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: CD14-positive monocytes were isolated from peripheral blood mononuclear cells (PBMCs) with CD14 microbeads and MACS LS column system (Miltenyi Biotec). PBMCs were obtained from the buffy coats of twelve healthy blood donors (eight males and four females ...
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bioRxiv - Immunology 2022Quote: Primary NK cells were enriched from normal human peripheral blood mononuclear cells (PBMCs) (Zenbio Inc.) using NK cell isolation kit (Miltenyi Biotec, 130-092-657). The purity of isolated NK cells was confirmed by staining of APC conjugated anti-human CD56 antibody and PE conjugated anti-human CD16 antibody ...
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bioRxiv - Immunology 2022Quote: Primary human CD4-positive T cells were isolated from enriched peripheral blood mononuclear cell (PBMC) fractions using the human CD4+ T cell isolation kit from Miltenyi Biotec (Bergisch Gladbach, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Primary CD4+ T lymphocytes were isolated from peripheral blood mononuclear cells and purified by negative selection using the CD4+ T cell isolation kit (Miltenyi Biotec, 130-096-533). Cells were phenotypically analyzed and activated using anti- CD3/antiCD28 coated plates (Ref BE0001-2 and Ref BE0248 ...
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bioRxiv - Immunology 2024Quote: ... CD14+ human monocytes were isolated from fresh peripheral blood mononuclear cells (PBMCs) using CD14 MACS MicroBeads (Miltenyi Biotec, Cat. No. 130-050-201) and cultured in MoDC differentiation medium at a density of 106 cells/ml for 6 days ...
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bioRxiv - Cancer Biology 2021Quote: ... Plasma cells were isolated from mononuclear blood cells using CD138 microbeads (Miltenyi Biotec, Inc.) according to the manufacturer’s instructions [89] ...
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bioRxiv - Biophysics 2023Quote: ... Peripheral mononuclear cells were collected by density gradient centrifugation and enriched using a CD8+ T cell Isolation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: CD34+ cells were isolated from umbilical cord blood or peripheral blood by positive selection using immunomagnetic bead cell-sorting system (AutoMacs; Miltenyi Biotec).
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bioRxiv - Cancer Biology 2021Quote: ... Peripheral blood B cells (PBBC) were isolated using the human Total B cell isolation kit II (Miltenyi Biotec) as per manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: Human primary CD4+ T cells are obtained from peripheral blood with CD4 T cell isolation kit II (Miltenyi Biotec). Unactivated CD4+ T cells were maintained in complete RPMI medium containing 50 U/ml interleukin 2 (IL-2 ...
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bioRxiv - Immunology 2019Quote: ... Pan T cells were isolated from peripheral blood leukocytes using the Pan T cell isolation kit according to manufacturer’s instructions (Miltenyi Biotec). Isolated human T cells were cultured in X-VIVO-15 medium supplemented with 2 % human serum.
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bioRxiv - Cell Biology 2023Quote: ... Pan B cells were isolated from peripheral blood leukocytes using the Pan B cell isolation kit according to manufacturer’s instructions (Miltenyi Biotec).
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bioRxiv - Genetics 2021Quote: Fresh G-CSF mobilized peripheral blood cells from healthy donor 1 were obtained from Miltenyi Biotec (Auburn, CA). CD34+ HSPCs were isolated using CliniMACS® CD34 reagent (Miltenyi ...
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bioRxiv - Immunology 2020Quote: ... Human CD4+ T-cells were isolated from peripheral blood lymphocytes using naïve CD4+ T-cell isolation kit from Miltenyi Biotec (Germany). The blood samples were obtained from Rheumatology Clinic at Saint Louis University with informed consent ...
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bioRxiv - Immunology 2022Quote: CD4+ T cells were isolated from peripheral blood lymphocytes by negative selection using a CD4 T-cell isolation kit II and magnetic cell separation (Miltenyi Biotec). A total of 50,000 CD4+ T cells were cultured in IMDM (Thermo Scientific ...
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bioRxiv - Cancer Biology 2021Quote: Control and constricted MDA-MB-231 cells were cocultured with human NK cells sorted from peripheral blood using the NK cell isolation Kit (Miltenyi Biotec 130-092-657) at the ratio of 1:20 ...
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bioRxiv - Systems Biology 2022Quote: Naïve CD8+ T cells were isolated from the peripheral blood of two healthy donors using the Naive CD8+ T Cell Isolation kit (Miltenyi Biotec, 130-093-244) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... PN were isolated from peripheral blood of healthy donors using MACSXPRESS Neutrophil Isolation Kit (Miltenyi Biotec), according to manufacturer protocol ...
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bioRxiv - Cell Biology 2021Quote: Human peripheral blood lymphocytes (PBLs) from a healthy donor were stimulated using the T Cell Activation/ Expansion Kit (MACS,Miltenyi Biotec, USA) according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2020Quote: Human CD34+ cells were purified from adult peripheral whole blood (LeukoPaks) by positive selection using microbeads magnetic cell separation system (Miltenyi Biotec, Cat # 130-100-453), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: CD34+ HSPC were enriched from cord blood mononuclear cells (MNC) by magnetic selection using anti-CD34 conjugated magnetic beads according to the manufacturer’s instructions (Miltenyi Biotec, 130-100-453). To obtain a sufficient cell number for ImageStream and Western blot analysis ...
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bioRxiv - Cell Biology 2022Quote: ... Human peripheral blood monocytes were isolated by the classical monocyte isolation kit (Miltenyi Biotec, #130-117-337), according to manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... GFP positive cells in freshly purified peripheral blood monocytes were isolated by positive selection using magnetic MicroBeads conjugated with F4/80 (#130-110-443, Miltenyi Biotec) and CD115 (#130-096-354 ...
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bioRxiv - Immunology 2021Quote: ... Human neutrophils were isolated from peripheral blood by negative selection with magnetic microspheres MACSxpress Neutrophil Isolation Kit and MACSxpress Separator (Miltenyi Biotec), according to the manufacturer instructions ...
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bioRxiv - Immunology 2022Quote: ... was drawn from peripheral veins of healthy volunteers and subjected to the MACSxpress Whole Blood Neutrophil Isolation Kit (#130-104-434, Miltenyi Biotec). The blood was incubated with labeling antibodies and placed in the magnetic separator ...
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bioRxiv - Cancer Biology 2022Quote: ... Red blood cells were removed by Red Blood Cell Lysis Solution kit (Miltenyi Biotec). Cell suspensions were washed with cold PBS ...
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bioRxiv - Developmental Biology 2020Quote: ... Red blood cells were removed with a red blood cell lysis solution (Miltenyi Biotec). Single-cell suspensions were incubated with a rat anti-CD49f antibody conjugated with phycoerythrin (ThermoFisher Scientific ...
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bioRxiv - Genomics 2024Quote: ... the red blood cells were lysed using Red Blood Cell Lysis Solution (Miltenyi Biotec), and dead cells were removed with Dead Cell Removal Kit (Miltenyi Biotec) ...
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bioRxiv - Cancer Biology 2022Quote: ... 5×105 mononuclear cells were blocked with human FcR blocking reagent (Miltenyi Biotec) for 5 min and stained in FACS buffer (Phosphate buffer saline - PBS plus 2 mM EDTA ...
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bioRxiv - Molecular Biology 2021Quote: ... red blood cell lysis was performed using the Red Blood Cell Lysis Solution (Miltenyi Biotec) following the manufacturer’s protocol that includes the washing step.
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bioRxiv - Microbiology 2021Quote: ... and further cleaned for red blood cells using red blood cell lysis buffer (Miltenyi Biotec). Countstar Fluorescence Cell Analyzer was used for single cells viability assessment ...
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Dendritic cells focus CTL responses toward highly conserved and topologically important HIV epitopesbioRxiv - Immunology 2020Quote: ... PBMC were thawed and monocytes and peripheral blood lymphocytes (PBL) were separated using human anti-CD14 Ab-coated microbeads to positively select monocytes (Miltenyi Biotec, Auburn, CA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... red blood cells were lysed with Red Blood Cells Lysis Solution (Miltenyi Biotec, 130-094-183) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... red blood cells were lysed with a Red Blood Cell Lysis kit (Miltenyi Biotec, 130-094-183), per manufacturer instructions ...
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Single cell transcriptomics reveals the effect of PD-L1/TGF-β blockade on the tumor microenvironmentbioRxiv - Genomics 2020Quote: ... A red blood cell lysis step was performed by incubating cells for 2 minutes with 1x Red Blood Cell Lysis Solution (Miltenyi Biotec). Cells were washed again and then cell viability was measured using the Nexcelom Cellometer K2 Fluorescent Viability Cell Counter and the ViaStain AOPI Staining Solution (Nexcelom) ...
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bioRxiv - Cancer Biology 2021Quote: ... red blood cells were removed using the Red Blood Cell Lysis Solution according to the manufacturer’s instructions (Miltenyi Biotec). Cells were then washed ...