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Citations for Miltenyi Biotec :
1 - 50 of 1611 citations for Nucleic Acid Purification kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... μMACS microcolumns were washed 1× with 100 μL nucleic acid equilibration buffer (Miltenyi Biotec), followed by 5× washes with 100 μL 1× wash buffer ...
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bioRxiv - Immunology 2021Quote: ... using an NK cell magnetic purification kit (Miltenyi Biotec). Murine NK were cultured in RPMI 1640 supplemented with 10% FCS ...
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bioRxiv - Immunology 2022Quote: ... pelleted splenocytes isolated from mice spleen were resuspended in FACS for NK cells purification with Mouse NK Cell Purification Kit (Miltenyi Biotec) according to the ...
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bioRxiv - Plant Biology 2019Quote: ... immuno-purification was performed with µMACS GFP Tag Protein Isolation kits (Miltenyi Biotec). 1 mg of solubilized microsomes were loaded on µcolumns ...
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bioRxiv - Pathology 2023Quote: ... followed by immunomagnetic separation using a human anti-CD14 purification kit (Miltenyi Biotec). The cell purity was always confirmed by flow cytometry and was superior to 95% ...
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bioRxiv - Immunology 2022Quote: ... and used for eosinophil purifications by negative selection on an AutoMACS Pro separator using the Eosinophil Purification Kit (Cat. No. 130-092-010, Miltenyi Biotec, Bergisch Gladbach, Germany). Eosinophil purity was >98% for all samples as determined by counting a minimum of 300 cells on cytospin slides stained with Shandon™ Diff-Kwik™ stains (Cat ...
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bioRxiv - Genomics 2022Quote: ... Naïve CD8a+ T cell purification was performed using Naïve CD8a+ T cell Isolation Kits (Miltenyi Biotec) according to the manufacturer’s protocols ...
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bioRxiv - Microbiology 2022Quote: ... We synchronized parasites to 80% schizont stages by purifications through MACS purification columns (Miltenyi Biotec): after overnight growth ...
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bioRxiv - Neuroscience 2020Quote: MACS purification of OPCs was performed according to the protocols in the kit instruction from Miltenyi Biotec. The mouse forebrain was dissociated using the papain dissociation system (LK003176 ...
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bioRxiv - Molecular Biology 2021Quote: ... for 90 min at room temperature and purification was performed using the μMACS streptavidin starting kit (Miltenyi Biotec). Columns were first equilibrated with 1 mL of washing buffer (100 mM Tris-HCl at pH 7.5 ...
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bioRxiv - Cancer Biology 2020Quote: ... MACS-based purification was performed with Myelin Removal Beads II followed by Mouse Cell Depletion kit (Miltenyi Biotec) according to manufacturer’s protocols ...
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bioRxiv - Immunology 2022Quote: ... and 5 mM EDTA and then isolated using mouse B cell purification kit (Miltenyi Biotec, 130-090-862) and LS columns (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: ... CD14+/CD16+ monocytes were isolated by negative selection magnetic bead column purification (Pan Monocyte Isolation Kit, Miltenyi Biotec). For chromatin accessibility (ATAC-seq) ...
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bioRxiv - Bioengineering 2024Quote: ... and 2 mM EDTA and then isolated using mouse B cell purification kit (Miltenyi Biotec, 130-090-862) and LS columns (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: ... cells were spun down at 500g for 10 minutes and resuspended in MACS buffer to purify PMNs with a magnetic purification kit according to manufacturer’s instruction (Miltenyi Biotec; Neutrophil Isolation Kit, Mouse).
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bioRxiv - Immunology 2019Quote: ... An antibody cocktail designed for negative selection of purified naïve T cells (Naïve Pan T cell purification kit, Miltenyi Biotec) was used together with extra αCD15/CD14 beads ...
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bioRxiv - Microbiology 2023Quote: ... NK cells were then re-purified by negative selection using purification kit and magnetic columns according to manufacturer’s instructions to reach purities of approximately 98% (Miltenyi Biotec, MS columns and isolation kit ref no.130-115-818) ...
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bioRxiv - Immunology 2023Quote: ... and the supernatant was discarded while PMs were purified according to manufacturer’s instruction using a magnet purification kit by selecting F4/80+ cells (Miltenyi Biotec; Macrophage Isolation Kit ...
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bioRxiv - Cell Biology 2022Quote: ... and magnet purification using LS columns (Miltenyi Biotec) and a MACS magnet ...
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bioRxiv - Cell Biology 2022Quote: ... and magnet purification using LS columns (Miltenyi Biotec) and a MACS magnet ...
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bioRxiv - Immunology 2022Quote: ... Cells were counted and CD11b+ cells were selected by MACS column purification using the CD11b MicroBead kit and LS columns (Miltenyi Biotec). CD11b+ cells were stained with Fc block α-CD16/32 (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... splenic B cells were enriched by magnetic microparticle purification using mouse B Cell Isolation Kit (Miltenyi Biotec, Cat#130-090-862) and then further sorted by gating CD11c+ B cells (CD19+CD11c+CD21- ...
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bioRxiv - Immunology 2022Quote: ... Cells were counted and CD11b+ cells were selected by MACS column purification using the CD11b MicroBead kit and LS columns (Miltenyi Biotec). CD11b+ cells were Fc blocked with α-CD16/32 (Biolegend ...
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bioRxiv - Cancer Biology 2023Quote: ... remaining cells were processed for T cell purification using the Pan T cell isolation kit (Miltenyi Biotec, cat # 130-095-130). Cell viability of the purified T cells was ascertained by labeling with the eBioscience™ Fixable Viability Dye eFluor™ 506 for 30 min on ice ...
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bioRxiv - Immunology 2020Quote: The exosomes extracted from SVV-green fluorescence protein (GFP)-infected cells and normal cells were purified using an exosome purification kit (Miltenyi Biotec, Bergisch Gladbach, Germany), and the copy number of SVV was detected ...
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bioRxiv - Microbiology 2024Quote: ... Samples were passed through μ Columns (MACS purification system, Miltenyi Biotec). The μ Columns were then washed 4 times with 200 μL of WASH buffer1 and 1 time with 200 μL of WASH buffer 2 ...
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bioRxiv - Immunology 2024Quote: ... MP1 and SARS-CoV-2 Prot_S (SPIKE) (peptides pools of 15 amino acids length with 11 amino acid overlap, Miltenyi Biotec) in 5% human serum RPMI medium in the presence of 1µg/ml anti-CD40 (HB14 ...
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bioRxiv - Immunology 2020Quote: ... sequences [SLA (p1-p53)] or 0.6 nmol/mL PepTivatorR Candida albicans MP65 (peptides pools of 15 amino acids length with 11 amino acid overlap, Miltenyi Biotec) in 5% human serum RPMI medium in the presence of 1µg/ml anti-CD40 (HB14 ...
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bioRxiv - Physiology 2021Quote: ... PBMCs purification was performed using anti-human CD14 magnetic beads (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... cells were either sub-cultured (Uns-C) or supplemented with kynurenic acid buffer and sorted according the manufacturer’s instructions using the Microbead Kit (Miltenyi Biotec #130-095-826). In brief ...
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bioRxiv - Genomics 2020Quote: ... 1:100 B27 supplement without retinoic acid (Miltenyi Biotec) supplemented with 10 µM SB-431542 ...
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bioRxiv - Cell Biology 2020Quote: ... Mitochondria were further purified by affinity purification using anti-TOM22 microbeads (Miltenyi Biotec) as per the manufacturers protocol with several modifications ...
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bioRxiv - Immunology 2020Quote: ... and monocytes were isolated by column purification using CD14 microbeads (Miltenyi Biotec, Somerville, MA). Cells were cultured for 1 week at a density of 106/well in 6 well plates with 50 ng/ml recombinant human macrophage colony stimulating factor (M-CSF ...
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bioRxiv - Plant Biology 2020Quote: ... amino acids 98-106 (Miltenyi Biotec, 130-091-972; 1:10.000) or anti-GFP (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... Monocytes were then purified from freshly isolated PBMCs using a CD14+ selection MACS purification (Miltenyi Biotec) followed by final re-suspension in chemotaxis buffer (Dulbecco’s modified Eagles medium (DMEM ...
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bioRxiv - Neuroscience 2022Quote: ... Further purification of microglia was performed through magnetic-activated bead sorting of CD11b+ cells (Miltenyi Biotec). This isolation method results in a culture of ∼97% PU.1+ ...
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bioRxiv - Microbiology 2021Quote: ... knowlesi tightly synchronized schizonts were obtained by magnetic purification using MACS column (Miltenyi Biotec, Bergisch Gladbach, Germany) (39) ...
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bioRxiv - Molecular Biology 2024Quote: ... Schizonts were isolated for imaging using magnetic purification (102) with a magnetic cell fraction column (MACS, Miltenyi Biotec) and a MidiMACS separator ...
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bioRxiv - Microbiology 2024Quote: ... consisting mainly of 15-mer sequences with 11-amino-acid (aa) overlap (Miltenyi Biotec). Negative controls for non-stimulated cells were included in the test ...
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bioRxiv - Immunology 2023Quote: ... Further purification of immune cells was done by magnetic enrichment of the CD45+ fraction (130-052-301, Miltenyi Biotec). All cell suspensions were kept in PEB buffer (0.5% BSA and 2 mM EDTA in PBS).
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bioRxiv - Microbiology 2021Quote: ... cultures of the two parasite lines for comparison were (i) synchronized to 80% late schizonts using MACS purification columns (Miltenyi Biotec) (ii ...
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bioRxiv - Microbiology 2024Quote: ... Samples were divided into equal parts (250 μL each) and incubated with 50 μL of magnetic microparticles coated with monoclonal HA or MYC antibodies (MACS purification system, Miltenyi Biotec) at 4°C for 1 hour under rotation (10 rpm) ...
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bioRxiv - Neuroscience 2022Quote: ... MACS NeuroBrew-21 with retinoic acid with final concentrations noted (10 mL, MACS Miltenyi Biotec, 130-093-566), PD0332991 (20 µM ...
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bioRxiv - Immunology 2021Quote: ... and PepTivator® SARS-CoV-2 Prot_S Complete peptide pool (Miltenyi Biotec, 15 mers with 11 amino acid overlap) covering the entire SARS-CoV-2 S protein ...
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bioRxiv - Cancer Biology 2024Quote: ... TYR or Mart-1/MLANA PepTivator peptide pools (15-mer peptide sequences with 11 amino acids overlap, Miltenyi Biotec) for 60 min at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... Kit (Miltenyi Biotec). Briefly ...
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bioRxiv - Immunology 2021Quote: ... Cells were cultured for 18 h at 5×105 cells per well with 2 μg/ml of a peptide pool consisting mainly of 15-mers (overlapping by 11 amino acids) covering the immunodominant sequence domains of the SARS-CoV-2 Spike protein (PepTivator® SARS-CoV-2 Prot_S; Miltenyi Biotec, Germany). The number of spots was determined using an automatic ELISPOT reader and image analysis software (CTL-ImmunoSpot® S6 Micro Analyzer ...
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bioRxiv - Immunology 2020Quote: ... following manufacturer instructions (Dead cell removal kit; Debris removal kit; Miltenyi Biotec) resulting in ~80% of purity and CD3+ T cells were resuspended in 1X PBS with 0.04% BSA ...
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bioRxiv - Cancer Biology 2019Quote: ... 2mM ethylenediaminetetracetic acid (EDTA) and PBS and incubated with either CD133-APC or CD133-VioBright FITC antibodies (both monoclonal mouse, Miltenyi Biotec, dilution 1:500) and FcR blocking reagent (Miltenyi Biotec ...
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bioRxiv - Plant Biology 2020Quote: ... the μMACS c-myc Isolation Kit and μMACS HA Isolation Kit (Miltenyi Biotec) were used according to the instructions provided ...