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Citations for Miltenyi Biotec :
1 - 50 of 537 citations for Macrophage metalloelastase MMP12 Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... This enriched macrophage/monocyte solution was incubated with human and mouse CD11b microbeads (Miltenyi Biotec; 130093-634), and cells were further enriched for Cd11b-positive macrophages/monocytes using Miltenyi MACS Separation Columns (LS ...
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bioRxiv - Immunology 2021Quote: ... and into M2-type macrophages by incubation with 100 ng/ml M-CSF (macrophage-colony stimulating factor, Miltenyi Biotec) in serum-free DC medium (CellGenix ...
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bioRxiv - Neuroscience 2023Quote: ... Magnetic beads coupled with mouse anti-CD11B antibodies (microglia/macrophage) were used for cell isolation according to the manufacturer’s protocol (Miltenyi Biotec) as previously described [29 ...
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bioRxiv - Physiology 2024Quote: ... Magnetic beads coupled with mouse anti-CD11B antibodies (microglia/macrophage) were used for cell isolation according to the manufacturer’s protocol (Miltenyi Biotec) as previously described [14 ...
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bioRxiv - Immunology 2022Quote: ... Macrophages were enriched using a magnetic-based commercial F4/80 macrophage positive selection kit (Miltenyi Biotec, MD, USA; Supplemental 3B) as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... αHis-PE (Miltenyi Biotec). Cytotect® (CP Biotest) ...
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bioRxiv - Microbiology 2020Quote: ... and granulocyte-macrophage colony-stimulating factor (Miltenyi Biotec) at 1,000 U/ml in order to stimulate differentiation to immature dendritic cells ...
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bioRxiv - Microbiology 2021Quote: ... were obtained by 7 days stimulation with 20 ng ml−1 macrophage colony-stimulating factor (M-CSF) and 10 ng ml−1 granulocyte-macrophage colony-stimulating factor (GM-CSF) (Miltenyi Biotec).
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bioRxiv - Microbiology 2021Quote: ... and differentiated to human monocyte-derived macrophages (hMDMs) by incubation with 100 ng/ml of recombinant human macrophage colony-stimulating factor (rhMCSF, Miltenyi Biotec) for 6 days at 37°C with 5% CO2 in a humidified atmosphere ...
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bioRxiv - Immunology 2022Quote: ... Peritoneal macrophages were isolated from adult (8-10 weeks old) female mice using Peritoneum Macrophage Isolation Kit (130-110-434, Miltenyi Biotec). Isolated cells were cultured in complete RPMI+GlutaMAX (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... Human monocytes were differentiated into a macrophage-like phenotype (MDM, monocyte derived macrophages) using M-CSF (50 ng/mL, Miltenyi Biotec, Bergisch Gladbach, Germany) for 6 days at 37ºC and 5% CO2 in Iscove’s Modified Dulbecco’s Medium (IMDM ...
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bioRxiv - Immunology 2021Quote: ... bone marrow of RBD-his tag protein immunized mice were isolated and purified with mouse CD138 MicroBeads (Miltenyi Biotec, 130-098-257) following standard protocol provided by the manufacturer ...
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bioRxiv - Cell Biology 2022Quote: ... and 25 ng/ml macrophage colony-stimulating factor (M-CSF; Miltenyi Biotec). HeLa and HEK 293 FT cells were maintained by bi-weekly subpassage in DMEM (Gibco ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... macrophages were purified using Cd11b microbeads (130-049-601) by Miltenyi Biotec, according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... 25ng/mL macrophage–colony stimulating factor (wt/vol, M-CSF, Miltenyi Biotec), and penicillin/streptomycin at 100U/0.1mg per mL of media respectively (wt/vol ...
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bioRxiv - Microbiology 2023Quote: ... with macrophage colony stimulating factor (M-CSF, 20 ng/mL; Miltenyi Biotec) (hereafter defined as complete medium) ...
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bioRxiv - Cancer Biology 2021Quote: ... and 1 ng/mL granulocyte macrophage colony-stimulating factor (GM-CSF) (Miltenyi Biotec). Primary cell cultures were maintained in the presence of mouse embryonic fibroblasts (STO ...
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bioRxiv - Immunology 2023Quote: ... Macrophages were isolated by magnetic cell isolation technology using CD11b microbeads (Miltenyi Biotec), according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... APC conjugated anti-HIS antibody (Miltenyi Biotec, Auburn, CA) was used as secondary antibody at 1:50 dilution ...
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bioRxiv - Biochemistry 2021Quote: ... APC conjugated anti-HIS antibody (Miltenyi Biotec, Auburn, CA) was used as secondary antibody at 1:50 dilution ...
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bioRxiv - Plant Biology 2023Quote: ... µMACS magnetic beads conjugated to anti-His (Miltenyi Biotec) were added and incubated for 1 h at 4°C to retrieve the bait protein together with interacting proteins ...
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bioRxiv - Immunology 2023Quote: ... then stained with fluorescent anti-His antibody (Miltenyi Biotec), along with other antibodies according to panels described in Supplementary Table 6 ...
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bioRxiv - Immunology 2022Quote: ... Isolated macrophages and monocytes were cultured in TexMACS medium (Miltenyi Biotec, 130-097-196) supplemented with 100 ng/ml human recombinant M-CSF (Peprotech ...
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bioRxiv - Immunology 2022Quote: ... anti-His-PE (mIgG1) (Miltenyi Biotec Swiss AG, Solothurn, Switzerland), and LIVE/DEAD™ Fixable Near-IR stain (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... and Anti-His-HRP (Miltenyi Biotec, Cat# 130-092-785).
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bioRxiv - Immunology 2020Quote: For cultured human lung macrophages staining: anti-CD169 (7-239) and anti-CD11c (REA618, Miltenyi Biotec) were used for membrane staining ...
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bioRxiv - Microbiology 2022Quote: ... and human granulocyte-macrophage colony-stimulating factor (GM-CSF; 20ng/ml; MILTENYI BIOTEC; 130-093-866). At day 5 ...
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bioRxiv - Cancer Biology 2023Quote: ... F4/8+ macrophages were isolated from these tumors using Anti-F4/80 MicroBeads UltraPure (Miltenyi Biotec), and co-cultured with the stimulated T cells (1 × 105 cells per well ...
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bioRxiv - Microbiology 2020Quote: ... 50 ng/ml recombinant human granulocyte macrophage colony–stimulating factor (rhGM-CSF) (130-093-865, Miltenyi Biotec), and 50 ng/ml rh interleukin (IL)-4 (130-093-921 ...
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bioRxiv - Plant Biology 2021Quote: ... To control for recombinant bacterial MCO expression anti-His-HRP (Miltenyi Biotec) was used (at a dilution of 1:10000 ...
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bioRxiv - Immunology 2019Quote: ... CD14+ alveolar macrophages were purified by positive selection using antibody-coated magnetic beads following manufacturer’s instructions (Miltenyi Biotec). Purity was checked by flow cytometry (Figure S1A ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with 10% fetal calf serum and 10 ng/ml granulocyte macrophage colony-stimulating factor (GM-CSF, Miltenyi Biotec) 61 ...
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bioRxiv - Plant Biology 2023Quote: ... and then with antibody (1:5000 anti-His-HRP (130-092-785, Miltenyi Biotec) or anti-FLAG-HRP (Ab49763 ...
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The antibiotic bedaquiline activates host macrophage innate immune resistance to bacterial infectionbioRxiv - Immunology 2019Quote: ... and were allowed to differentiate into Mφs in the presence of granulocyte macrophage colony-stimulating factor (GM-CSF, 20 ng/mL; Miltenyi Biotec) over a 6-day period ...
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bioRxiv - Bioengineering 2021Quote: ... The general surface marker composition for the sEVs from the three cell sources (HEK293, cbMSC, THP1) was assessed by a multiplex bead-based flow cytometry assay (MACSPlex Exosome Kit, human, Miltenyi Biotec) by using an optimized workflow as described previously.[27] In brief ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were harvested and the transfection efficiency as well as expression of the NSD2 constructs in HEK293 cells were evaluated based on the mVenus reporter by flow cytometry (MACSQuant VYB, Miltenyi Biotec) and western blot using an anti GFP antibody (ThermoFisher ...
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bioRxiv - Immunology 2019Quote: Blood-derived macrophages were resuspended in 100uL FACS buffer (PBS with 1% FBS) and stained with FITC-conjugated CD87/PLAUR (VIM5-Miltenyi Biotec), acquired by FACSCanto (BD Biosciences ...
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bioRxiv - Neuroscience 2021Quote: ... Three mice of 3-6 months were euthanized and subjected to a peritoneal lavage with PBS to extract macrophages subsequently purified using CD11b+ magnetic beads (Cat. 130-049-601, Miltenyi Biotec), following the operative manual ...
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bioRxiv - Immunology 2021Quote: ... 21 and 63 DPI samples (n=3) were depleted of alveolar macrophages using a MACS cell separation LS column (Miltenyi Biotec) after staining for CD14 and CD172a (see Table S2) ...
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bioRxiv - Physiology 2023Quote: ... CD11b/c positive cells (monocyte/macrophage) were sorted using magnetic-activated cell sorting (MACS) separation columns (Miltenyi Biotec, 130-105-634) and immediately lysed with TRIzol (Fischer Scientific ...
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bioRxiv - Microbiology 2021Quote: ... cells were stained with Anti-His-PE (clone: GG11-8F3.5.1; Miltenyi Biotec; Cat. #130-120-787) for 30 min at 4°C ...
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TAD border deletion at the Kit locus causes tissue-specific ectopic activation of a neighboring genebioRxiv - Genetics 2022Quote: ... For the Hi-C experiment Kit positive cells were magnetically separated (Miltenyi Biotec, 130-097-146).
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bioRxiv - Neuroscience 2023Quote: ... was used to isolate microglia/macrophages from ipsilateral (injured hemisphere) cortical and hippocampal tissue using Miltenyi MACS Separation Technology (Miltenyi Biotec, Auburn, CA) as per manufacturers instructions ...
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bioRxiv - Immunology 2024Quote: ... to obtain monocyte-derived macrophages which were purified with magnetic bead separation (CD14 negative isolation protocol, Monocyte Isolation Kit II. Miltenyi Biotec, Auburn, CA). Isolated CD14+ monocyte-derived macrophages (MDMs ...
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bioRxiv - Immunology 2022Quote: ... Alveolar-like MDMs were generated by differentiating CD4+ monocytes on tissue culture plates into macrophages in the presence of 50 ng/ml of human M-CSF (Miltenyi Biotec, Bergisch Gladbach, Germany) for 6 days ...
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bioRxiv - Cancer Biology 2019Quote: ... mouse (Miltenyi Biotec) at 4°C for 5 min ...
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bioRxiv - Physiology 2021Quote: ... mouse (Miltenyi Biotec). The cells were sorted into cooled sterile PBS+10%FBS ...
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bioRxiv - Physiology 2021Quote: ... mouse (Miltenyi Biotec), anti-CD29(ITGB1)-APC ...
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bioRxiv - Immunology 2020Quote: ... mouse (Miltenyi Biotec). The purity of B cells was determined by flow-cytometry (>95%) ...
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bioRxiv - Immunology 2022Quote: ... mouse (Miltenyi Biotec). CytoFLEX (Beckman ...