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Citations for Miltenyi Biotec :
1 - 50 of 616 citations for Lymphocyte antigen 6H LY6H Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... we also used CD3-PE to specifically identify lymphocytes (human antibody, clone BW264/56, Miltenyi Biotec). For all the samples ...
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bioRxiv - Immunology 2024Quote: ... CD3+ T lymphocytes and CD20+ B lymphocytes were sorted from PBMCs using CD3+ and CD20+ microbeads (Miltenyi Biotec) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... Human CD4+ T-cells were isolated from peripheral blood lymphocytes using naïve CD4+ T-cell isolation kit from Miltenyi Biotec (Germany). The blood samples were obtained from Rheumatology Clinic at Saint Louis University with informed consent ...
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bioRxiv - Microbiology 2021Quote: ... 1x105 HEK-293T cells were mixed at a 1:1 ratio in 96-well plates with primary CD4+ T lymphocytes freshly isolated from healthy donors by negative selection (CD4+ T-Cell Isolation Kit II, human, Miltenyi Biotec). Viral transfer was assessed after 24 hours of incubation at 37°C in permeabilized (FIX & PERM Cell Permeabilization kit ...
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bioRxiv - Microbiology 2023Quote: ... were gained from EDTA blood by density separation (Lymphocyte Separation Medium (AnproTech)) and CD8+ T-cells were isolated with the human CD8+ T-cell isolation kit (Miltenyi Biotec) and a MidiMACS LS-column according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: Human peripheral blood lymphocytes (PBLs) from a healthy donor were stimulated using the T Cell Activation/ Expansion Kit (MACS,Miltenyi Biotec, USA) according to manufacturer instructions ...
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Dendritic cells focus CTL responses toward highly conserved and topologically important HIV epitopesbioRxiv - Immunology 2020Quote: ... PBMC were thawed and monocytes and peripheral blood lymphocytes (PBL) were separated using human anti-CD14 Ab-coated microbeads to positively select monocytes (Miltenyi Biotec, Auburn, CA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... lymphocytes were counted using a MACSQuant flow cytometer (Miltenyi Biotec), using propidium iodide (PI ...
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bioRxiv - Bioengineering 2021Quote: ... The general surface marker composition for the sEVs from the three cell sources (HEK293, cbMSC, THP1) was assessed by a multiplex bead-based flow cytometry assay (MACSPlex Exosome Kit, human, Miltenyi Biotec) by using an optimized workflow as described previously.[27] In brief ...
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bioRxiv - Immunology 2022Quote: ... (3) Non-SARS-CoV-2 antigens: Peptide pools of the pp65 protein of human cytomegalovirus (CMV) (Miltenyi Biotec, Gladbach, GER), or HIV-1 gag peptide pools (NIH AIDS Reagent Repository ...
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Ex vivo Immune Profiling in Patient Blood enables Quantification of innate immune Effector FunctionsbioRxiv - Immunology 2020Quote: ... Surgery-induced changes on monocyte surface antigen expression were analysed for HLA-DR (anti-human HLA-DR, clone REA805, Miltenyi Biotec) and CD62L ...
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bioRxiv - Immunology 2022Quote: ... Hepatic lymphocytes were isolated using Liver dissociation kit (Miltenyi Biotec, Auburn, CA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... CD8+ T lymphocytes were isolated using immunomagnetic beads (MACS beads; Miltenyi Biotec). The cells were cultured in RPMI 1640 medium supplemented with 10% human serum and 10 IU/mL human rIL-2 ...
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bioRxiv - Microbiology 2020Quote: ... αHis-PE (Miltenyi Biotec). Cytotect® (CP Biotest) ...
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bioRxiv - Immunology 2020Quote: B lymphocytes were isolated from mouse spleens using anti-CD43 MicroBeads (Miltenyi Biotec), and grown in RPMI 1640 medium (Life Technologies ...
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bioRxiv - Microbiology 2023Quote: ... SC lymphocytes were extracted using a brain dissociation kit (Miltenyi Biotec, #130-107-677), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: Resting B lymphocytes were isolated from mouse spleens using anti-CD43 MicroBeads (Miltenyi Biotec), and grown in RPMI 1640 medium (Life Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... and subsequently stained at 4°C with antibodies against following human antigens: CD3 (REA613, APC-Vio770, Miltenyi Biotec, Cat no.130-113-698, 1:50), CD4 (A161A1 ...
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bioRxiv - Cancer Biology 2019Quote: ... The transfected TI lymphocytes were stimulated with the anti-CD3 antibody (1 μg/mL, OKT3, Miltenyi Biotec) coated on the plate for 84 h ...
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bioRxiv - Immunology 2023Quote: ... B lymphocytes were enriched from erythrocyte-lysed spleen cells using CD43 MicroBeads (Miltenyi Biotec,130-049-801) and LS columns (Miltenyi Biotec ...
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bioRxiv - Molecular Biology 2021Quote: Nave B lymphocytes were purified from mouse spleens by negative selection using CD43 (Ly48) magnetic beads (Miltenyi Biotec) according to the manufacturer’s instructions. ...
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bioRxiv - Cell Biology 2023Quote: ... Erythrocytes were lysed in RBC lysis buffer and lymphocytes were depleted by MACS using CD45 microbeads (Miltenyi Biotec) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: Circulating CD8+ T lymphocytes were isolated from fresh heparin blood by negative selection using magnetic beads (Miltenyi Biotec) from PBMC ...
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bioRxiv - Microbiology 2023Quote: ... While CLP and ILP lymphocytes were extracted using the lamina propria dissociation kit (Miltenyi Biotec, #130-097-410), SC lymphocytes were extracted using a brain dissociation kit (Miltenyi Biotec ...
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bioRxiv - Molecular Biology 2021Quote: Resting primary B lymphocytes (wild-type and Tp53bp1-/-) were purified from mouse spleens using anti-CD43 microbeads (Miltenyi Biotec). One million cells were stimulated to proliferate with a cytokine cocktail containing 25 μg/mL lipopolysaccharide (LPS ...
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bioRxiv - Cell Biology 2021Quote: T lymphocytes were isolated from healthy donor’s PBMCs via Pan T Cell Isolation Kit (Miltenyi Biotec,130-096-535) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... APC conjugated anti-HIS antibody (Miltenyi Biotec, Auburn, CA) was used as secondary antibody at 1:50 dilution ...
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bioRxiv - Biochemistry 2021Quote: ... APC conjugated anti-HIS antibody (Miltenyi Biotec, Auburn, CA) was used as secondary antibody at 1:50 dilution ...
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bioRxiv - Plant Biology 2023Quote: ... µMACS magnetic beads conjugated to anti-His (Miltenyi Biotec) were added and incubated for 1 h at 4°C to retrieve the bait protein together with interacting proteins ...
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bioRxiv - Immunology 2023Quote: ... then stained with fluorescent anti-His antibody (Miltenyi Biotec), along with other antibodies according to panels described in Supplementary Table 6 ...
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bioRxiv - Immunology 2021Quote: ... Half of the livers were processed to enrich lymphocytes according to manufacturer’s instructions with a gentleMACS Dissociator in C tubes (Miltenyi Biotec). Alternatively ...
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bioRxiv - Immunology 2021Quote: ... and monocytes were separated from lymphocytes by positive selection using magnetic cell sorting technique according to the manufacturer’s instructions (Miltenyi Biotec) and as described (34).
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bioRxiv - Biochemistry 2022Quote: Monocyte-and lymphocyte-enriched PBMC suspensions were sorted using Isolation kits and Depletion Column Type LS (Miltenyi Biotec Inc., Auburn, CA) according to the supplier’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Fluorescence spillover compensation for cell sorting was performed using lymphocytes stained with CD4 (OKT4) in the respective colors and with compensation beads (Miltenyi Biotec) for flow cytometry ...
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bioRxiv - Immunology 2022Quote: CD4+ T cells were isolated from peripheral blood lymphocytes by negative selection using a CD4 T-cell isolation kit II and magnetic cell separation (Miltenyi Biotec). A total of 50,000 CD4+ T cells were cultured in IMDM (Thermo Scientific ...
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bioRxiv - Immunology 2023Quote: Single cell preparations of tumor-infiltrating lymphocytes were made by mechanically disrupting and enzymatically digesting tumors using gentleMACS™ (Miltenyi Biotec). The digestion media contained collagenase IV and dispase II (1 mg/ml each ...
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bioRxiv - Immunology 2023Quote: A cluster of differentiation 19 (CD19)-positive B lymphocytes (total B cells) were purified from buffy coats derived from healthy donors using magnetic microbeads (Miltenyi Biotec, Bergisch Gladbach ...
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bioRxiv - Immunology 2022Quote: ... anti-His-PE (mIgG1) (Miltenyi Biotec Swiss AG, Solothurn, Switzerland), and LIVE/DEAD™ Fixable Near-IR stain (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... and Anti-His-HRP (Miltenyi Biotec, Cat# 130-092-785).
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bioRxiv - Bioengineering 2021Quote: ... Nalm6 tumors were extracted on day 4 post bacteria treatment (day 2 post T cell treatment) and lymphocytes were isolated from tumor tissue by mechanical homogenization using a gentleMACS™ Dissociator (Miltenyi Biotec) in complete hTcm ...
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bioRxiv - Microbiology 2021Quote: ... B lymphocyte isolation was conducted through negative selection by using the B cell Isolation Kit II (Miltenyi Biotec, Cat#130-091-151) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... Tregs were purified from the mouse lymphocytes using a CD4+CD25+ regulatory T cell isolation kit (Miltenyi Biotec, cat. # 130-091-041). The purity of the isolated cells was checked by flow cytometry ...
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bioRxiv - Genetics 2022Quote: ... anti-human-CD19 and anti-human-CD14 (Miltenyi Biotec) and transferred through LD columns (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2023Quote: ... Naïve CD8+ T cells were then purified from total lymphocytes by negative selection according to the manufacturer’s instructions (Miltenyi Biotec, 130-096-543) and cultured in DMEM containing 10% FBS ...
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bioRxiv - Immunology 2020Quote: ... Afterwards antigen specific CD8+ T cells were isolated using IFN-γ secretion assay (Miltenyi Biotec). Single cells were seeded into individual wells (1 cell/200 μl in each well ...
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bioRxiv - Plant Biology 2021Quote: ... To control for recombinant bacterial MCO expression anti-His-HRP (Miltenyi Biotec) was used (at a dilution of 1:10000 ...
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bioRxiv - Immunology 2023Quote: ... Naïve CD8 T lymphocytes were purified from total PBMC by Naïve CD8+ T Cell Isolation Kit and MACS® separation columns (Miltenyi Biotec, Bergisch Gladbach, Germany), following manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... human (Miltenyi Biotec), per manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... human (Miltenyi Biotec) protocol ...
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bioRxiv - Cell Biology 2020Quote: ... human (Miltenyi Biotec), according to the manufacturer’s instructions [44].