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1 - 50 of 509 citations for Legumain Asparaginyl Endopeptidase Mouse HEK293 His since 2019

• Human Cytomegalovirus antagonizes activation of Fcγ receptors II and III by distinct and synergizing modes of IgG manipulation

  Philipp Kolb, et al., bioRxiv - Microbiology 2020

  Quote: ... αHis-PE (Miltenyi Biotec). Cytotect® (CP Biotest) ...
• Monospecific and bispecific monoclonal SARS-CoV-2 neutralizing antibodies that maintain potency against B.1.617

  Lei Peng, et al., bioRxiv - Immunology 2021

  Quote: ... bone marrow of RBD-his tag protein immunized mice were isolated and purified with mouse CD138 MicroBeads (Miltenyi Biotec, 130-098-257) following standard protocol provided by the manufacturer ...
• Structural basis for enhanced infectivity and immune evasion of SARS-CoV-2 variants

  Yongfei Cai, et al., bioRxiv - Biochemistry 2021

  Quote: ... APC conjugated anti-HIS antibody (Miltenyi Biotec, Auburn, CA) was used as secondary antibody at 1:50 dilution ...
• Membrane fusion and immune evasion by the spike protein of SARS-CoV-2 Delta variant

  Jun Zhang, et al., bioRxiv - Biochemistry 2021

  Quote: ... APC conjugated anti-HIS antibody (Miltenyi Biotec, Auburn, CA) was used as secondary antibody at 1:50 dilution ...
• Initiation of B-type starch granules in wheat endosperm requires the plastidial α-glucan phosphorylase PHS1

  Nitin Uttam Kamble, et al., bioRxiv - Plant Biology 2023

  Quote: ... µMACS magnetic beads conjugated to anti-His (Miltenyi Biotec) were added and incubated for 1 h at 4°C to retrieve the bait protein together with interacting proteins ...
• Reprogramming human B cells with custom heavy chain antibodies

  Geoffrey L. Rogers, et al., bioRxiv - Immunology 2023

  Quote: ... then stained with fluorescent anti-His antibody (Miltenyi Biotec), along with other antibodies according to panels described in Supplementary Table 6 ...
• Single-cell transcriptomics reveals striking heterogeneity and functional organization of dendritic and monocytic cells in the bovine mesenteric lymph node

  G.T. Barut, et al., bioRxiv - Immunology 2022

  Quote: ... anti-His-PE (mIgG1) (Miltenyi Biotec Swiss AG, Solothurn, Switzerland), and LIVE/DEAD™ Fixable Near-IR stain (Thermo Fisher Scientific ...
• Oxidoreductases generate hydrogen peroxide that drives iron-dependent lipid peroxidation during ferroptosis

  Bo Yan, et al., bioRxiv - Biochemistry 2020

  Quote: ... and Anti-His-HRP (Miltenyi Biotec, Cat# 130-092-785).
• Bacterial-type plant ferroxidases tune local phosphate sensing in root development

  Christin Naumann, et al., bioRxiv - Plant Biology 2021

  Quote: ... To control for recombinant bacterial MCO expression anti-His-HRP (Miltenyi Biotec) was used (at a dilution of 1:10000 ...
• Protein migration and topography mapping reveals endogenous protein processing and homomers in Nicotiana benthamiana

  Kaijie Zheng, et al., bioRxiv - Plant Biology 2023

  Quote: ... and then with antibody (1:5000 anti-His-HRP (130-092-785, Miltenyi Biotec) or anti-FLAG-HRP (Ab49763 ...
• Multiparametric Profiling of Single Nanoscale Extracellular Vesicles by Combined Atomic Force and Fluorescence Microscopy: Correlation and Heterogeneity in Their Molecular and Biophysical Features

  Sara Cavallaro, et al., bioRxiv - Bioengineering 2021

  Quote: ... The general surface marker composition for the sEVs from the three cell sources (HEK293, cbMSC, THP1) was assessed by a multiplex bead-based flow cytometry assay (MACSPlex Exosome Kit, human, Miltenyi Biotec) by using an optimized workflow as described previously.[27] In brief ...
• The T1150A cancer mutant of the protein lysine dimethyltransferase NSD2 can introduce H3K36 trimethylation

  Mina S. Khella, et al., bioRxiv - Molecular Biology 2023

  Quote: ... cells were harvested and the transfection efficiency as well as expression of the NSD2 constructs in HEK293 cells were evaluated based on the mVenus reporter by flow cytometry (MACSQuant VYB, Miltenyi Biotec) and western blot using an anti GFP antibody (ThermoFisher ...
• Characterization of SARS-CoV-2 variants B.1.617.1 (Kappa), B.1.617.2 (Delta) and B.1.618 on cell entry, host range, and sensitivity to convalescent plasma and ACE2 decoy receptor

  Wenlin Ren, et al., bioRxiv - Microbiology 2021

  Quote: ... cells were stained with Anti-His-PE (clone: GG11-8F3.5.1; Miltenyi Biotec; Cat. #130-120-787) for 30 min at 4°C ...
• TAD border deletion at the Kit locus causes tissue-specific ectopic activation of a neighboring gene

  Evelyn Kabirova, et al., bioRxiv - Genetics 2022

  Quote: ... For the Hi-C experiment Kit positive cells were magnetically separated (Miltenyi Biotec, 130-097-146).
• Cancer cell CCR2 orchestrates suppression of the adaptive immune response

  Miriam R. Fein, et al., bioRxiv - Cancer Biology 2019

  Quote: ... mouse (Miltenyi Biotec) at 4°C for 5 min ...
• Single cell transcriptome mapping identifies a local innate B cell population driving local antibody production and chronic rejection after lung transplantation

  Natalia F. Smirnova, et al., bioRxiv - Physiology 2021

  Quote: ... mouse (Miltenyi Biotec). The cells were sorted into cooled sterile PBS+10%FBS ...
• Single cell transcriptome mapping identifies a local innate B cell population driving local antibody production and chronic rejection after lung transplantation

  Natalia F. Smirnova, et al., bioRxiv - Physiology 2021

  Quote: ... mouse (Miltenyi Biotec), anti-CD29(ITGB1)-APC ...
• Genetic timestamping of plasma cells in vivo reveals homeostatic population turnover

  AQ Xu, RR Barbosa, DP Calado, bioRxiv - Immunology 2020

  Quote: ... mouse (Miltenyi Biotec). The purity of B cells was determined by flow-cytometry (>95%) ...
• Balancing activation and costimulation of CAR tunes signaling dynamics and enhances therapeutic potency

  Yanting Duan, et al., bioRxiv - Immunology 2022

  Quote: ... mouse (Miltenyi Biotec). CytoFLEX (Beckman ...
• Cell Communication Network factor 4 promotes tumor-induced immunosuppression in melanoma

  Audry Fernandez, et al., bioRxiv - Cancer Biology 2021

  Quote: ... mouse (Miltenyi Biotec) from the spleens of CCN4-KO and YUMM1.7-WT TB mice and tumor-free mice ...
• Cell Communication Network factor 4 promotes tumor-induced immunosuppression in melanoma

  Audry Fernandez, et al., bioRxiv - Cancer Biology 2021

  Quote: ... mouse (Miltenyi Biotec) at a 1:1 ratio with anti-CD3/anti-CD28-loaded beads ...
• Cell Communication Network factor 4 promotes tumor-induced immunosuppression in melanoma

  Audry Fernandez, et al., bioRxiv - Cancer Biology 2021

  Quote: ... mouse (Miltenyi Biotec) and the manufacturer’s instructions ...
• Collagen Density Modulates the Immunosuppressive Functions of Tumor-Associated Macrophages

  Anne Mette H. Larsen, et al., bioRxiv - Immunology 2019

  Quote: ... mouse (Miltenyi Biotec). Briefly ...
• Single cell transcriptome mapping identifies a local innate B cell population driving local antibody production and chronic rejection after lung transplantation

  Natalia F. Smirnova, et al., bioRxiv - Physiology 2021

  Quote: ... mouse (Miltenyi Biotec), PE anti-mouse CD43 (BD Pharmingen) ...
• Single cell transcriptome mapping identifies a local innate B cell population driving local antibody production and chronic rejection after lung transplantation

  Natalia F. Smirnova, et al., bioRxiv - Physiology 2021

  Quote: ... mouse (Miltenyi Biotec), anti-CD29(ITGB1)-APC ...
• MIF-2/D-DT is an atypical atherogenic chemokine that promotes advanced atherosclerosis and hepatic lipogenesis

  Omar El Bounkari, et al., bioRxiv - Immunology 2021

  Quote: ... Mouse (Miltenyi Biotec), according to the manufacturer’s protocol ...
• Single-cell transcriptomics of allo-reactive CD4⁺ T cells over time reveals divergent fates during gut GVHD

  Jessica A. Engel, et al., bioRxiv - Immunology 2020

  Quote: ... Mouse (Miltenyi Biotec), according to the manufacturer’s protocol.
• Diet prevents the expansion of segmented filamentous bacteria and ileo-colonic inflammation in a model of Crohn’s disease

  Amira Metwaly, et al., bioRxiv - Microbiology 2022

  Quote: ... mouse (Miltenyi Biotec) and intracellular staining was performed by using eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set (Thermo Fisher Scientific).
• Endothelial cell-derived lactate triggers mesenchymal stem cell histone lactylation to attenuate osteoporosis

  Jinhui Wu, et al., bioRxiv - Cell Biology 2023

  Quote: ... mouse (Miltenyi Biotec). Sorted BMECs were seeded on fibronectin (sigma-Aldrich ...
• Clec12a tempers inflammation while restricting expansion of a colitogenic commensal

  Tyson R. Chiaro, et al., bioRxiv - Immunology 2023

  Quote: ... mouse (Miltenyi Biotec) and an LS MACS column according to manufacturer instructions.
• Ikzf1 association with Foxp3 for Foxp3-dependent gene repression in Treg cells: induction of autoimmunity and tumor immunity by disrupting the association

  Kenji Ichiyama, et al., bioRxiv - Immunology 2023

  Quote: ... mouse (Miltenyi Biotec). Enriched cells were stained with Live/Dead cell viability dye and antibodies for surface markers as follows ...
• Natural killer cell regulation of breast cancer stem cells mediates metastatic dormancy

  Grace G. Bushnell, et al., bioRxiv - Cancer Biology 2023

  Quote: ... mouse (Miltenyi Biotec) for magnetic activated cell sorting using the MACS system (Miltenyi Biotec) ...
• Natural killer cell regulation of breast cancer stem cells mediates metastatic dormancy

  Grace G. Bushnell, et al., bioRxiv - Cancer Biology 2023

  Quote: ... mouse (Miltenyi Biotec) for magnetic activated cell sorting using the MACS system (Miltenyi Biotec) ...
• Murine Parainfluenza Virus Persists in Lung Innate Immune Cells Sustaining Chronic Lung Pathology

  Ítalo Araujo Castro, et al., bioRxiv - Microbiology 2023

  Quote: ... mouse (Miltenyi Biotec) isolation kit ...
• Differential regulation by CD47 and thrombospondin-1 of extramedullary erythropoiesis in mouse spleen

  Rajdeep Banerjee, et al., bioRxiv - Cell Biology 2023

  Quote: ... mouse (Miltenyi Biotec). Single cell suspensions were incubated with the supplied antibody cocktail of the CD8+ T cell isolation kit for 15 min ...
• An mRNA-encoded, long-lasting Interleukin-2 restores CD8⁺ T cell neoantigen immunity in MHC class I-deficient cancers

  Jan D. Beck, et al., bioRxiv - Immunology 2023

  Quote: ... mouse (Miltenyi Biotec), according to the manufacturer’s instructions.
• An mRNA-encoded, long-lasting Interleukin-2 restores CD8⁺ T cell neoantigen immunity in MHC class I-deficient cancers

  Jan D. Beck, et al., bioRxiv - Immunology 2023

  Quote: ... mouse (Miltenyi Biotec) or CD8 (TIL ...
• An mRNA-encoded, long-lasting Interleukin-2 restores CD8⁺ T cell neoantigen immunity in MHC class I-deficient cancers

  Jan D. Beck, et al., bioRxiv - Immunology 2023

  Quote: ... mouse (Miltenyi Biotec) in combination with a gentleMACSTM Dis-sociator (Miltenyi Biotec ...
• An mRNA-encoded, long-lasting Interleukin-2 restores CD8⁺ T cell neoantigen immunity in MHC class I-deficient cancers

  Jan D. Beck, et al., bioRxiv - Immunology 2023

  Quote: ... mouse (Miltenyi Biotec) according to the manufacturer’s protocol ...
• An mRNA-encoded, long-lasting Interleukin-2 restores CD8⁺ T cell neoantigen immunity in MHC class I-deficient cancers

  Jan D. Beck, et al., bioRxiv - Immunology 2023

  Quote: ... mouse (Miltenyi Biotec), respectively ...
• Primary and recurrent glioma patient-derived orthotopic xenografts (PDOX) represent relevant patient avatars for precision medicine

  Anna Golebiewska, et al., bioRxiv - Cancer Biology 2020

  Quote: ... Mouse cells were removed with Mouse Cell Depletion kit (Miltenyi Biotec). Tumor cells were seeded 1000 cells/well in organoid medium in 384-well plates (PrimeSurface® ...
• Primary and recurrent glioma patient-derived orthotopic xenografts (PDOX) represent relevant patient avatars for precision medicine

  Anna Golebiewska, et al., bioRxiv - Cancer Biology 2020

  Quote: ... Mouse cells were removed with Mouse Cell Depletion kit (Miltenyi Biotec). Tumor cells were mixed with 1% alginate (ratio of 1:1 ...
• ACVR1 antibodies exacerbate heterotopic ossification in fibrodysplasia ossificans progressiva (FOP) by activating FOP-mutant ACVR1

  Senem Aykul, et al., bioRxiv - Biochemistry 2021

  Quote: ... ACVR1-binding antibodies were enriched by incubation of biotinylated ACVR1-Fc and Myc-His monomeric ACVR1 at different concentrations with antibody expressing yeast cells followed by magnetic bead selection (Miltenyi Biotec) or flow cytometry on an Aria II cell sorter (BD Biosciences ...
• Epithelial-mesenchymal plasticity determines estrogen receptor positive (ER+) breast cancer dormancy and reacquisition of an epithelial state drives awakening

  Patrick Aouad, et al., bioRxiv - Cancer Biology 2021

  Quote: ... mouse cells were depleted using the mouse cell depletion kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
• LY6E protects mice from pathogenic effects of murine coronavirus and SARS-CoV-2

  Katrina B. Mar, et al., bioRxiv - Microbiology 2023

  Quote: ... mouse (Miltenyi Biotec, Germany). Lung homogenates were filtered through a 70 μM cell strainer ...
• LY6E protects mice from pathogenic effects of murine coronavirus and SARS-CoV-2

  Katrina B. Mar, et al., bioRxiv - Microbiology 2023

  Quote: ... mouse (Miltenyi Biotec, Germany). Lung homogenates were filtered through a 70 μM cell strainer ...
• Tumor-derived biomarkers beyond antigen expression enhance efficacy of CD276/B7H3 antibody-drug conjugate in metastatic prostate cancer

  Supreet Agarwal, et al., bioRxiv - Cancer Biology 2023

  Quote: ... depleted for mouse cells using mouse cell depletion kit (Miltenyi Biotec, manufacturer’s protocol), and plated as organoids according to our previously described methods (21) ...
• Early induction of cytokine release syndrome by rapidly generated CAR T cells in a preclinical mouse model

  Naphang Ho, et al., bioRxiv - Pharmacology and Toxicology 2022

  Quote: ... and mouse-derived samples were additionally blocked with mouse FcR-blocking reagent (Miltenyi Biotec). The following anti-human antibodies were used for FACS staining ...
• Exogenous extracellular matrix proteins decrease cardiac fibroblast activation in stiffening microenvironment through CAPG

  Xinming Wang, et al., bioRxiv - Bioengineering 2021

  Quote: ... mouse and rat (Miltenyi Biotec). Cardiomyocytes and non-cardiomyocytes were separated by Percoll gradient centrifugation ...
• MyoD-Cre driven alterations in K-Ras and p53 lead to a mouse model with histological and molecular characteristics of human rhabdomyosarcoma with direct translational applications

  Kengo Nakahata, et al., bioRxiv - Cancer Biology 2021

  Quote: ... mouse (Miltenyi Biotec, Cologne, Germany) and gentleMACS Dissociator (Miltenyi Biotec) ...