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Citations for Miltenyi Biotec :
1 - 50 of 515 citations for Iron Sulfur Cluster Co Chaperone Protein HscB Mitochondrial HSCB Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... CD14+ monocytes were purified using anti-human CD14 antibody-labeled magnetic beads and iron-based LS columns (Miltenyi Biotec) and used directly for further differentiation into macrophages (Dominguez-Soto et al. ...
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bioRxiv - Molecular Biology 2019Quote: ... Co-immunoprecipitated proteins were isolated using magnetic μMACS columns (Miltenyi Biotec) as previously described61 ...
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bioRxiv - Microbiology 2019Quote: ... CD14+ monocytes were purified using anti-human CD14 antibody-labeled magnetic beads and iron-based LS columns (Miltenyi Biotec, Auburn, CA) and used immediately for further differentiation into macrophages [25] ...
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bioRxiv - Genomics 2023Quote: ... (21) using MACS mitochondrial isolation kit (Miltenyi Biotec, #130-094-532). Briefly ...
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bioRxiv - Plant Biology 2020Quote: ... Co-immunoprecipitation was conducted using the μMACS™ Epitope Tag Protein Isolation Kits according to manufacturer’s instructions (Miltenyi Biotec), and purified on magnetic μ Column (Miltenyi Biotec) ...
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bioRxiv - Plant Biology 2019Quote: ... RPS5A::mCherry-PDK1.2 were used for Co-IP assays using a µMACS Epitope Tag Protein Isolation Kit (Miltenyi Biotec), with RPS5A::mCherry-PDK1.1 and RPS5A::mCherry-PDK1.2 transgenic lines as controls ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were cultured in clusters and passaged every 4-5 days using StemMACS Dissociation reagent (Miltenyi Biotec). All iPS lines were genotyped using an RFLP at the relevant important loci and the sequence was confirmed by Sanger sequencing ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were then co-incubated with 1 μg of APC-conjugated anti-IgG antibody (Miltenyi Biotec; Bergisch Gladbach, Germany) and 0.5 μg of PE-conjugated anti-Flag antibody (Miltenyi Biotec ...
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bioRxiv - Bioengineering 2024Quote: ... MS Columns (Miltenyi Biotec B.V. & Co. KG), and a MiniMACS Separator (Miltenyi Biotec B.V ...
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bioRxiv - Cancer Biology 2022Quote: ... and 37°C incubator and passaged every 4-5 days in small clusters using StemMACS Dissociation reagent (Miltenyi Biotec). Colon organoids were differentiated as previously described[46] with minor modifications ...
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bioRxiv - Plant Biology 2023Quote: ... Protein-DNA complexes were immunoprecipitated using anti-HA antibodies (Miltenyi Biotec) (Kaufmann et al. ...
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bioRxiv - Neuroscience 2023Quote: Mitochondrial pellets were collected from HV and PD cell samples using Mitochondria Isolation Kit (Miltenyi Biotec, Germany). Mitochondrial proteins were extracted using Nuclear Extract Kit (Active Motif ...
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bioRxiv - Bioengineering 2024Quote: ... and a MiniMACS Separator (Miltenyi Biotec B.V. & Co. KG) were used according to the manufacturer’s protocols.
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bioRxiv - Bioengineering 2021Quote: ... an LS column (130-042-401; Miltenyi Biotec B.V. & Co. KG) was placed into the magnetic field of an QuadroMACS Separator (130-090-976 ...
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bioRxiv - Cell Biology 2021Quote: ... Antibodies were captured with 100µl of μMACS protein A beads (Miltenyi Biotec; San Diego, CA). Beads were isolated with μ columns and a μMACS separator (Miltenyi Biotec ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins were detected with horseradish peroxidase (HRP)-conjugated anti-GFP antibody (1:5,000; Miltenyi Biotec) for one hour at room temperature ...
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bioRxiv - Plant Biology 2021Quote: ... H-tagged proteins were detected using the anti-HA HRP-coupled antibody (mouse monoclonal, Miltenyi Biotec) diluted 1:5000 (v/v) ...
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bioRxiv - Plant Biology 2022Quote: ... Proteins were subsequently detected with horseradish peroxidase (HRP)-conjugated anti-GFP antibody (1:5,000) (Miltenyi Biotec) for 1 hr at room temperature with gentle shaking ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were subsequently detected with horseradish peroxidase (HRP)-conjugated anti-GFP antibody (1:5,000) (Miltenyi Biotec) or HRP-conjugated anti-c-myc antibody (1:5,000 ...
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bioRxiv - Biochemistry 2020Quote: ABCB1 co-immunoprecipitation analyses using anti-GFP micro beads (Miltenyi Biotec, Germany) were carried using microsomal fractions prepared from Agrobacterium-co-infiltrated N ...
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bioRxiv - Plant Biology 2020Quote: ... Eluted proteins were analysed by western blot using HRP-conjugated anti-myc antibody (130-092-113; Miltenyi Biotec), or an anti-HA antibody (3F10 ...
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bioRxiv - Bioengineering 2021Quote: ... 1:20 in autoMACS Rinsing Solution (130-091-222; Miltenyi Biotec B.V. & Co. KG). For degassing ...
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bioRxiv - Molecular Biology 2021Quote: The co-immunoprecipitations were performed using µMACS HA or GFP Isolation Kit (Miltenyi Biotec) according to manufacturer protocol using 10×106 cells and 50 µL of antibody-coupled magnetic beads in a volume of 1 mL ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were subsequently detected with horseradish peroxidase (HRP)-conjugated anti-GFP antibody (1:5,000) (Miltenyi Biotec #130-091-833), anti-plant actin mouse monoclonal antibody (3T3)-HRP (Abbkine # A01050HRP) ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were detected with anti-TuMV coat protein antibody at a concentration of 1:1500 and Goat anti-Rabbit IgG (H+L)-HRP conjugate (Miltenyi Biotec) at a concentration of 1:10,000 ...
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bioRxiv - Immunology 2020Quote: ... and the complete sequence of the nucleocapsid phosphoprotein N and the membrane glycoprotein M (GenBank MN908947.3, Protein QHD43416.1, Protein QHD43423.2, Protein QHD43419.1) (Miltenyi Biotec, Germany). After 5 hours of stimulation ...
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bioRxiv - Plant Biology 2020Quote: ... for co-immunoprecipitation with FLAG-RBOHD and with 200 μL of anti-HA magnetic beads (Miltenyi Biotec) for co-immunoprecipitation with PB1CP-HA ...
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bioRxiv - Bioengineering 2021Quote: ... was placed into the magnetic field of an QuadroMACS Separator (130-090-976; Miltenyi Biotec B.V. & Co. KG) and primed by rinsing with 3 ml of MACS buffer ...
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bioRxiv - Immunology 2023Quote: In vitro cytotoxicity assays were performed by co-culturing sorted CD8 T-cells (Miltenyi Biotec catalogue # 130-104-075) with tumor cells in the presence or absence of antigen-pulsed macrophages ...
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bioRxiv - Plant Biology 2022Quote: ... Co-immunoprecipitation analyses were carried out as described recently using anti-GFP MicroBeads (Miltenyi Biotec, Germany; (Henrichs et al., 2012)) except that bands of interest were size-selected by Flamingo fluorescent gel stain (BioRad ...
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bioRxiv - Plant Biology 2020Quote: ... Immunoprecipitation to isolate the protein complexes from total protein extracts using GFP-tagged microbeads (Miltenyi Biotec) has been described previously [8] ...
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bioRxiv - Pathology 2019Quote: ... Increased ratios of MDSCs and nonMDSCs were co-cultured with splenic T cells isolated with Pan T Cell Isolation Kit II (Miltenyi Biotec) and stained with 1 μM Carboxyfluorescein succinimidyl ester (CFSE ...
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The tumour microenvironment shapes dendritic cell plasticity in a human organotypic melanoma culturebioRxiv - Immunology 2019Quote: ... Autologous or allogenic CD3+ T cells used in T cell co-culture experiments were isolated using the Pan T cell isolation kit (Miltenyi Biotec) following manufacturer’s instruction.
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bioRxiv - Immunology 2021Quote: ... were co-transferred with CD3+ cell-depleted autologous PBMCs (2 × 106) isolated using CD3 Microbeads and the LD Column (Miltenyi Biotec) into 5- to 7-week-old female mice ...
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bioRxiv - Immunology 2020Quote: ... Sorted cell subsets were co-cultured at a ratio 1:1 in 200µL RPMI/10% FBS and stimulated with CytoStim™ (Miltenyi Biotec). After 7 days’ culture ...
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bioRxiv - Immunology 2020Quote: ... PolyPeptide group) were co-cultured with 105 OTII CD4+ T cells (purified with a CD4+ T cell isolation kit, Miltenyi Biotec) in RPMI medium (ThermoFisher ...
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bioRxiv - Immunology 2021Quote: ... and microglia was isolated with the Papain-based Neural Tissue Dissociation Kit (P) (# 130-092-628, Miltenyi Biotec B.V. & Co. KG) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... in 96- well U-bottom plates 24 hours prior to co-culture with the indicated combinations of growth/differentiation factors (10 ng/ml each; all from Miltenyi Biotec). UCB or BM CD34+ HSPC subsets were directly seeded by FACS in the corresponding plates and cultured for 10 (bulk cultures ...
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bioRxiv - Immunology 2022Quote: ... in 96-well U-bottom plates 24 hours prior to co-culture with the indicated combinations of growth/differentiation factors (10 ng/ml each; all from Miltenyi Biotec). CD34+ HSPCs isolated from the BM of xenografted mice sacrificed between weeks 1-5 after grafting were directly seeded by FACS in the 96-well plates and cultured for 7 or 14 days before quantification of lineage outputs ...
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bioRxiv - Molecular Biology 2023Quote: Drosophila S2 cells grown in SFX medium (HyClone) at 25°C were co-transfected by plasmid expressing MSL1*-3xHA and plasmid expressing MSL2-3xFLAG with MACSFectin (Miltenyi Biotec), as recommended by the manufacturer ...
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bioRxiv - Cell Biology 2021Quote: ... Interacting proteins were isolated by applying total protein extracts to α-GFP-coupled magnetic beads (Miltenyi Biotec). Three replicates of TPLATE motif substitution mutants (SWM and PFM ...
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bioRxiv - Bioengineering 2021Quote: ... MACS buffer was prepared freshly before each isolation by diluting MACS BSA Stock Solution (130-091-376; Miltenyi Biotec B.V. & Co. KG) 1:20 in autoMACS Rinsing Solution (130-091-222 ...
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bioRxiv - Cell Biology 2022Quote: ... After an additional 24h co-cultured cells were trypsinized and endothelial cells were purified using CD31 microbead kit (Miltenyi Biotec #130-091-935) according to manufacturer instructions.
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bioRxiv - Immunology 2022Quote: ... The antibody cocktails included anti-mouse antibodies from Miltenyi Biotec: CD44-FITC (clone IM7.8.1) ...
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bioRxiv - Plant Biology 2019Quote: ... Interacting proteins were isolated from total protein extracts using anti-GFP-coupled magnetic beads (Miltenyi Biotec, Bergisch Gladbach, Germany). Proteins were digested with trypsin to peptides ...
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bioRxiv - Neuroscience 2021Quote: ... we prepared single-cell suspensions from spleens of 4- and 12-month-old APPtgp38fl/flLysM-Cre+/- and APPtgp38fl/flLysM-Cre-/- littermate mice with the Spleen Dissociation Kit (Miltenyi Biotec B.V. & Co. KG). After blocking with 25 μg/ml rat anti-mouse CD16/CD32 antibody (clone 2.4G2 ...
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bioRxiv - Bioengineering 2021Quote: ... CD14+-cells were isolated by magnetic activated cell sorting (MACS) with positive selection using CD14 MicroBeads (130-050-201; Miltenyi Biotec B.V. & Co. KG) from freshly isolated SVF or PBMCs according to supplier’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... CD3/CD28-mediated activation of T cells was conducted by using 10 μl of T cell TransActTM (130-111-160; Miltenyi Biotec B.V. & Co. KG) according to manufacturer’s instructions (T cell activation medium) ...
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bioRxiv - Plant Biology 2020Quote: ... and proteins eluted with Elution Buffer (Miltenyi Biotec). Protein extracts were the subjected to immunoblot analyses using α-myc or α-HA antibodies.
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bioRxiv - Microbiology 2022Quote: ... MultiMACS protein A/G microbeads (Miltenyi Biotec, Germany) were used to catch the secreted proteins as described by[35].