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Citations for Miltenyi Biotec :
1 - 50 of 1068 citations for Human Chemokine C X C Motif Receptor 5 CXCR5 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Fc receptors were blocked for 10 minutes at 4°C with FcR blocking reagent (MACS Miltenyi Biotec). Cells harvested during the conversion period from CD34+ hematopoietic precursors to CD14+ monocytes (Days 12-21 of the differentiation protocol ...
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bioRxiv - Cell Biology 2023Quote: ... After stopping the lysis with 1X PBS+10%FCS, cells were pelleted by centrifugation (300 x g, 5 min, 4°C) and subjected to dead cell removal (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... in 5 mL of IMDM+Glutamax medium for 30 min at 37°C in gentleMACS C tubes (Miltenyi Biotec). During and after incubation ...
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bioRxiv - Neuroscience 2019Quote: ... human recombinant anti-CD11b/c (1:50) (#130-120-288, Miltenyi Biotec, Germany). Secondary antibodies ...
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bioRxiv - Immunology 2022Quote: ... After centrifugation (5 min, 500 x g, 4 °C) supernatant was discarded and cells were resuspended in MACS buffer (Miltenyi Biotec, #130-091-221) for following cell sorting.
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bioRxiv - Microbiology 2019Quote: ... Proteins were immunoprecipitated by adding 30ul of α-c-Myc magnetic beads (μMACS Anti-c-myc Miltenyi Biotec, cat# 130-091-284) and incubated for 2h at 4°C with rotation ...
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bioRxiv - Plant Biology 2021Quote: ... Proteins were immunoprecipitated by adding 150 ul of α-c-HA magnetic beads (µMACS Anti-c-myc Miltenyi Biotec, cat# 130-094-255) as described above ...
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bioRxiv - Plant Biology 2021Quote: ... Proteins were immunoprecipitated by adding 30 µl of α-c-Myc magnetic beads (µMACS Anti-c-myc Miltenyi Biotec, cat# 130-091-284) and incubated for 2 h at 4°C with rotation ...
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bioRxiv - Cancer Biology 2023Quote: ... at 4°C and distributed at 5 mL per LS column (Miltenyi Biotec), or 5×106 BM MNC were thawed ...
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bioRxiv - Genetics 2022Quote: ... in a cell culture incubator (37 °C; 5% CO2) followed by dissociation in gentleMACS C Tubes (Miltenyi Biotec Inc., cat. no. # 130-093-237) in a total of 8 ml DMEM media with 10% FBS using gentleMACS Dissociator Program A twice and seeded onto 15 cm tissue culture dishes and grown in DMEM with 10% FBS at 37 °C with 5% CO2.
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bioRxiv - Immunology 2020Quote: ... Fc receptor were blocked with human FcR Blocking Reagent (120-000-442, Miltenyi Biotec) and anti-CD16/32 (clone 2.4G2 ...
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bioRxiv - Microbiology 2019Quote: ... using C tubes (Miltenyi Biotec) in ice cold Dulbecco’s PBS supplemented with 0.1% BSA ...
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bioRxiv - Immunology 2022Quote: ... A Fc receptor (FcR) blocking step was performed using FcR Blocking Reagent Human (Miltenyi Biotec) before cell surface antibody staining ...
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bioRxiv - Pathology 2022Quote: ... Hearts were digested for 30 min at 37°C in gentleMACS C Tubes (Miltenyi Biotec) with 800 U/ml Collagenase II and 100 U/ml DNase I (Worthington Biochemical Corporation) ...
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bioRxiv - Cell Biology 2021Quote: ... and 60 ng/mL of human receptor activator of NF-κB-ligand (RANK-L, Miltenyi Biotec). The medium was then changed every third day by RPMI containing 10% FCS ...
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bioRxiv - Pathology 2023Quote: ... and homogenized at 37°C in MACS C-tubes using the gentle MACS dissociator (Miltenyi Biotec), digestion neutralized with PEB buffer (2 mM EDTA ...
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bioRxiv - Cancer Biology 2023Quote: ... Pre-enrichment for c-kit+ cells was achieved using anti-c-kit magnetic beads (Miltenyi Biotec).
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bioRxiv - Immunology 2020Quote: ... and in C Tubes (Miltenyi Biotec) disassociated using the gentleMACS Octo Dissociator (Miltenyi Biotec) ...
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bioRxiv - Microbiology 2020Quote: ... using a C-Tube (Miltenyi Biotec), which allows dissociation of viable single cells from tissue samples ...
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bioRxiv - Cell Biology 2022Quote: ... transferred into C-tubes (Miltenyi Biotec), and processed in digestion buffer (1 mg/ml of Collagenase D and 80 U/ml DNase I ...
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bioRxiv - Immunology 2023Quote: ... in gentleMACS C tubes (Miltenyi Biotec) and enzymes from the Mouse Tumor Dissociation Kit (Miltenyi Biotec) ...
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bioRxiv - Cancer Biology 2024Quote: ... in gentleMACS C Tubes (Miltenyi Biotec) for 42 min at 37 °C using the gentleMACS Octo Dissociator ...
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bioRxiv - Cancer Biology 2020Quote: ... c-Kit+ cells were enriched using magnetic beads conjugated with an anti-c-Kit antibody (Miltenyi Biotec), transduced with a recombinant retrovirus by spinoculation ...
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bioRxiv - Immunology 2023Quote: ... PBMC were incubated at 37°C for 15 minutes with 0.5 μg/mL anti-human CD40 mAb blocking antibody (Miltenyi Biotec). Chemokine receptor antibodies were also added on day 1 (see ref23 for antibodies used in the AIM assay) ...
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bioRxiv - Immunology 2020Quote: ... Fc receptors were blocked for 5 min at RT using FcR-blocking reagent (Miltenyi Biotec) and then incubated with fluorochrome-conjugated surface antibodies for 15 min at RT ...
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bioRxiv - Cell Biology 2021Quote: ... BM cells were pre-enriched for c-Kit+ cells using c-Kit microbeads (Miltenyi Biotec, 130-091-224) and an AutoMACS cell separator (Miltenyi Biotec) ...
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bioRxiv - Immunology 2021Quote: ... then transferred to C tubes (Miltenyi Biotec), containing 5 ml of RPMI-1640 (Sigma R8758 ...
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bioRxiv - Immunology 2023Quote: ... transferred to gentleMACSTM C Tubes (Miltenyi Biotec) prefilled with 2.5 mL of the enzyme mix prepared in RPMI1640 ...
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bioRxiv - Cancer Biology 2021Quote: ... Fresh tissue was cut into 1 mm pieces and dissociated at 37°C using the Human Tumor Dissociation Kit (Miltenyi Biotec) on a gentleMACS Octo Dissociator ...
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bioRxiv - Immunology 2022Quote: The tissue pieces were rapidly transferred into the gentleMACS C Tube containing Human Tumor Dissociation Kit (Miltenyi Biotec, #130-095-929) according to the manufacturer’s recommendation ...
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bioRxiv - Microbiology 2023Quote: ... at 37°C for 30 mins were added to fresh human neutrophils extracted from blood from healthy volunteers using MACSxpress kit (Miltenyi Biotec) at an MOI of 1:100 (72 ...
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bioRxiv - Molecular Biology 2024Quote: ... Each sample was dissociated for 15 min at 37 °C on the GentleMACS Octo Dissociator with Heaters (Miltenyi) using Human Tumor Dissociation Kit (Miltenyi Biotec). Following tissue dissociation ...
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bioRxiv - Cancer Biology 2024Quote: ... and cryopreserved in FBS supplemented with 10% dimethyl sulfoxide (DMSO) at -80 °C (samples for genotyping and mass cytometry) or processed directly using Tumor Dissociation Kit (human, Miltenyi Biotec) as described below (fresh sample for mass cytometry) ...
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bioRxiv - Cancer Biology 2022Quote: ... and 37°C incubator and passaged every 4-5 days in small clusters using StemMACS Dissociation reagent (Miltenyi Biotec). Colon organoids were differentiated as previously described[46] with minor modifications ...
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bioRxiv - Developmental Biology 2021Quote: ... Differentiating c-KIT+ neonate spermatogonia cells were magnetically labeled with CD117 (c-KIT+) MicroBeads (Miltenyi Biotec 130-091-224) and isolated using MS columns (Miltenyi Biotec 130-042-201 ...
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bioRxiv - Molecular Biology 2023Quote: ... The protein complexes were then eluted into 1.5 mL Eppendorf tubes using 3 x 35 µL of pre-heated 95°C elution buffer (Miltenyi Biotec #130-042-701). The samples were heated to 95°C for 5 min ...
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bioRxiv - Cell Biology 2019Quote: ... Human ES cells were maintained at 37°C in StemMACS(tm) iPS-Brew XF media (Miltenyi Biotec GmbH, cat#130-104-368), supplemented with penicillin ...
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bioRxiv - Cell Biology 2022Quote: ... washed in Phosphate-Buffered Saline (PBS) and placed in C-tubes (gentleMACS C Tubes, Miltenyi Biotec Inc., San Diego, CA) and digested with collagenase and dispase as previously described.12,24 The resulting mixture was filtered through a 45 µm cell filter (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Lungs were placed in C tubes (Miltenyi Biotec) containing 2.2 mL Buffer S ...
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bioRxiv - Immunology 2023Quote: ... digested in gentle MACSTM C Tubes (Miltenyi Biotec) with an enzyme mixture (Liberase ...
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bioRxiv - Cancer Biology 2023Quote: ... gentleMACS C tubes (Miltenyi Biotec, 130-093-237), and gentleMACS Octo Dissociator with heaters (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: ... mechanical disassociation using GentleMACS C tubes (Miltenyi Biotec) and program m_intestine_01 on the gentleMACS dissociator (Miltenyi Biotec ...
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bioRxiv - Microbiology 2023Quote: ... and transferred to gentleMACS C-tubes (Miltenyi Biotec) before mounting on a GentleMACS dissociator (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: ... transferred to a gentleMACS C Tube (Miltenyi Biotec), washed twice with basal media containing 10 µM Y-27632 ...
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bioRxiv - Immunology 2019Quote: ... before culturing in a 1:10 ratio with 5×105 BALB/c splenic APCs (prepared via Thy1.2+ cell depletion by MACS, Miltenyi Biotec) in a 96-well round-bottom plate in complete RPMI medium for 5 days ...
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bioRxiv - Immunology 2022Quote: ... 5 ng/ml human IL-7 (Miltenyi Biotec), and 5 ng/ml human FLT3L (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... and 5 ng/ml human FLT3L (Miltenyi Biotec) as described previously 50.
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bioRxiv - Immunology 2023Quote: ... human TGFβ-1 (5 ng/ml, Miltenyi Biotec), anti-mouse IL-4 (11B11 ...
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bioRxiv - Neuroscience 2024Quote: ... The resulting cell suspension was spun down for 5 min at 300 × g and 4°C and processed for MACS using the CD11b/c microbeads according to the manufacturers protocol (Miltenyi Biotec). Subsequently ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were stained with APC-efluo780 conjugated anti-c-kit antibody and c-kit+ cells were enriched using anti-APC paramagnetic microbeads (Miltenyi Biotec). Cells were stained with fluorophore-conjugated antibodies against lineage markers (CD2 ...