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Citations for Miltenyi Biotec :
1 - 50 of 2053 citations for Glutathione Fluorescent Detection Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... The "Mouse Tregs detection kit" (Miltenyi Biotec®, Bergisch Gladbach, Germany) was used for regulatory T-cell staining ...
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bioRxiv - Molecular Biology 2019Quote: Apoptosis was analyzed using Annexin V-FITC apoptosis detection kit (Miltenyi Biotec). MDA-MB-231 and MCF-7 were incubated with recombinant IFNε for 48 h ...
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bioRxiv - Immunology 2019Quote: ... the cells were harvested and stained using the Treg detection kit (Miltenyi Biotec), resuspended in FACS buffer (1x PBS supplemented with 3% heat-inactivated and filtered FCS and 0.1%NaN3 ...
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bioRxiv - Immunology 2021Quote: ... Lineage Cell Detection Cocktail-Biotin (Miltenyi Biotec, #130-092-613, 1:100), Anti-Biotin MicroBeads (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... we used human IFNγ secretion assay detection kit (PE) (Miltenyi Biotec, #130-054-202) as per the manufacturer’s protocol ...
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bioRxiv - Immunology 2019Quote: ... The staining procedure was executed as per the manufacturer instructions (Treg Detection Kit, Miltenyi Biotec). The cells were resuspended in FACS buffer (1x PBS supplemented with 3% heat-inactivated and filtered FCS and 0.1%NaN3 ...
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bioRxiv - Cancer Biology 2020Quote: Fluorescent antibodies against Gr1 (Miltenyi Biotec), CD11b (BD) ...
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bioRxiv - Microbiology 2019Quote: ... Cells (106) were stained with the ‘Mouse IFN-γ secretion assay detection kit’ (Miltenyi Biotec, Germany) according to the manufacturer’s instructions and IFN-γ secretion was analyzed by flow cytometry.
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bioRxiv - Immunology 2023Quote: ... IFN-γ-secreting cells were detected using IFN-γ Secretion Assay/Detection Kit (PE) (Miltenyi Biotec) according to the manufacturer’s instructions and subsequently phenotypically characterised by flow cytometry.
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bioRxiv - Immunology 2020Quote: ... the cells were labeled with the IFN-γ Catch Reagent (IFN-γ Secretion Assay-Detection Kit, human Miltenyi Biotec) containing bispecific antibodies for CD45 and IFN-γ which was secreted by the stimulated target cells ...
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bioRxiv - Immunology 2023Quote: ... then stained with fluorescent anti-His antibody (Miltenyi Biotec), along with other antibodies according to panels described in Supplementary Table 6 ...
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bioRxiv - Microbiology 2019Quote: ... or IFN-γ detection antibody (Miltenyi Biotec, Germany). After washing the cells ...
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bioRxiv - Cancer Biology 2020Quote: ... the population of IFNγ secreting cells was identified using the IFNγ Secretion assay detection kit (PE) (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... such T cells were selected using a human IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (Miltenyi Biotec, Auburn, CA) following the manufacturer’s instructions as previously described.38–42 Post-4 PBMC sample cryopreserved after monocyte depletion was thawed and cultured overnight in Yssel’s media (Gemini Bio Products ...
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bioRxiv - Immunology 2023Quote: ... purified CD8+ T-cells were coated with mouse IL-2 catch reagent from a mouse IL-2 secretion assay detection kit (Miltenyi Biotec) by incubating T cells in a 20x dilution of IL-2 catch reagent in R10 medium for 15 min on ice ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Myc for CAR detection (SH1-26e7.1.3, FITC, Miltenyi Biotec), CD14 (REA599 ...
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bioRxiv - Immunology 2020Quote: ... plates were blocked with 1% BSA in PBS solution (Miltenyi Biotec, Bergisch Gladbach, Germany) for two hours at room temperature ...
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Inhibition of major histocompatibility complex-I antigen presentation by sarbecovirus ORF7a proteinsbioRxiv - Microbiology 2022Quote: ... PBMCs were stimulated for 3 hours with 10 μg/mL of Gag AK11 peptide and T cells producing IFN-γ in response were enriched using the IFN-γ Secretion Detection and Enrichment Kit (130-054-201; Miltenyi Biotec, Bergisch Gladbach, Germany) in accordance with the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Lin–biotin (Lineage Cell Detection Cocktail-Biotin, Miltenyi Biotec, #130-092-613), Streptavidin–PE (BD Biosciences ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: PBMCs were activated in plates coated with 1 μg/mL anti-CD3 mAb (clone: OKT3, Miltenyi Biotec) and cultured in the presence of 3 μg/mL anti-CD28 mAb (clone ...
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bioRxiv - Immunology 2024Quote: ... Two hundred thousand T cells per well were cultured in 96-well plates in the presence of T Cell Activation/Expansion Kit (Miltenyi Biotec) for 2 weeks ...
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bioRxiv - Genomics 2023Quote: ... Multi Tissue Dissociation Kit 1 and gentleMACS Octo Dissociator (Miltenyi Biotec) were used for dissociation of the tissue into single-cell suspension with a reduced amount of enzyme R (25% of the regular amount) ...
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bioRxiv - Neuroscience 2019Quote: ... plates in StemMACs culture medium (Miltenyi Biotec). The medium was changed every day and the cells were passaged every 5-6 days using PBS without Calcium and Magnesium (Life Technologies).
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bioRxiv - Molecular Biology 2020Quote: PBMC were obtained from healthy donors and were cultured in 48-well plates at a density of 1 × 106/ml in TexMACS (Miltenyi Biotec), 3% human serum (Seralab ...
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bioRxiv - Immunology 2020Quote: ... plated in 6-well culture plates at a concentration of 5 × 106 cells/ml in 1 ml of serum-free TexMACS medium (Miltenyi Biotec) and left overnight (5 % CO2 ...
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bioRxiv - Immunology 2022Quote: ... cells were resuspended in 96-well U-bottom plates and blocked prior to staining with 1 μg/mL anti–CD40 (clone HB14, Miltenyi Biotec) for 30 minutes at 37°C ...
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bioRxiv - Bioengineering 2024Quote: Splenocytes were plated in 96-well U-bottom plates at 106 cells/well in RPMI and stimulated with 1 μg/mL of PepTivator Ovalbumin (Miltenyi Biotec). After 3 hours ...
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bioRxiv - Cancer Biology 2022Quote: 24-well tissue culture-treated plates were pre-coated with 1 µg/mL anti-CD3 (OKT3) antibody (Miltenyi Biotec 130-093-387) in 1 mL PBS per well ...
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bioRxiv - Microbiology 2020Quote: ... iPSCs were maintained on Geltrex (Thermofisher Scientific)-coated plates in iPS-Brew XF (Miltenyi Biotec) media at 37°C in 5% CO2/5% O2/90% air humidified atmosphere ...
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bioRxiv - Cell Biology 2020Quote: Astrocytes were purified using the Anti-GLAST (ACSA-1) MicroBead Kit (Miltenyi biotec). Briefly ...
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bioRxiv - Immunology 2020Quote: ... each sample was divided into two tubes for either cytokine detection with IFN-γ-FITC (Miltenyi Biotec), IL-2-PE (Miltenyi Biotec ...
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bioRxiv - Plant Biology 2020Quote: ... 1% (v/v) Nonidet P-40) and incubated for 2 h with anti-green fluorescent protein (GFP) magnetic beads (MACS / Miltenyi Biotec) at 4 °C under rotation ...
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bioRxiv - Immunology 2021Quote: ... Open-repertoire T cells (7.5e106 cells per well of a 24 well plate) were stimulated with OKT3 (50 ng mL−1, Miltenyi Biotec, Cat 130-093-387) and rhIL-2 (300 IU mL−1 ...
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bioRxiv - Cancer Biology 2020Quote: ... and subsequently incubated with anti-CD117/c-kit-APC (1:50; Miltenyi Biotec; RRID: AB_615056). Cells were fixed in 1% formalin and analyzed on a BD FACS Canto II running the FACS Diva Software (BD Biosciences) ...
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bioRxiv - Immunology 2023Quote: ... U48 and Z11 samples (Supplementary Table 1) using a magnetic sorting kit from Miltenyi Biotec including LS Columns (130-042-401 ...
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bioRxiv - Genetics 2020Quote: ... BM freshly isolated mononuclear cells (MNC) were first stained with Lineage Cell Detection Cocktail-Biotinylated mouse antibody (MACS Miltenyi Biotec). For FACS analysis ...
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bioRxiv - Cell Biology 2021Quote: ... coated plates using StemMACS™ iPS-Brew XF medium (Miltenyi Biotec) and passaged with 0.5mM EDTA (Invitrogen) ...
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bioRxiv - Developmental Biology 2020Quote: ... coated plates in StemMACS™ iPS-Brew XF medium (Miltenyi Biotec). Cells were cultured in clusters and passaged every 4-5 days using StemMACS Dissociation reagent (Miltenyi Boitec ...
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bioRxiv - Cell Biology 2020Quote: ... coated plates in StemMACS™ iPS-Brew XF medium (Miltenyi Biotec). Cells were cultured in clusters and passaged every 4-5 days using StemMACS Dissociation reagent (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2021Quote: ... –coated plates and cultured in StemMACS iPS-Brew XF (Miltenyi Biotec) with 5 μ After 24 h ...
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bioRxiv - Cell Biology 2021Quote: ... in phosphate-buffered saline (Thermo Fisher Scientific)–coated plates and cultured in StemMACS iPS-Brew XF (Miltenyi Biotec) at 37 °C and 5% CO2 ...
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bioRxiv - Microbiology 2023Quote: ... Cells were stained extracellularly with fluorescent antibodies listed in Supplementary Table S2 and the fixable ViobilityTM Live/Dead Dye (Miltenyi Biotec, Bergisch Gladbach, Germany). After incubation in the dark for 15 min at 4 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... Fluorescent intensity of 10 000 cells was detected by flow cytometry on a FACS instrument (MACSQuant®VYB, Miltenyi Biotec, San Diego, CA, USA). Data were analyzed using FlowJo software (FlowJo LLC ...
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bioRxiv - Cell Biology 2022Quote: ... was designed and prepared in 96-well glass bottom plates (Miltenyi Biotec) using a protocol for solid phase reverse transfection as previously described16,42 ...
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bioRxiv - Cell Biology 2024Quote: ... stem cells were cultured on Matrigel (Corning 354230)-coated plates in StemMACS iPS-Brew XF (Miltenyi Biotec 130-104-368). The cell culture media was changed every other day and cells were passaged at a split ratio between 1 ...
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bioRxiv - Microbiology 2021Quote: ... dead cells were removed from the 1 mL cell suspension using a dead cell removal kit (Miltenyi Biotec) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... minced and digested using the Tissue Dissociation Kit 1 and a gentleMACS™ Dissociator (both from Miltenyi Biotec). Cells were filtered through a 70 μm cell strainer ...
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bioRxiv - Molecular Biology 2022Quote: ... the same method was followed with the substitution of the fluorescent antibody mix against tetraspanins (CD63, CD81 and CD9) with the APC labelled HLA-G antibody (130111852, Miltenyi Biotec, same volume of 15 µl). This method ensured that EVs attached to the Ab conjugated beads were only detected if positive for HLA-G.
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bioRxiv - Neuroscience 2020Quote: ... Microglia were isolated from adult mouse brain using the multi tissue dissociation kit 1 (Miltenyi Biotec #130-110-201), followed by CD11b microglia isolation kit (Miltenyi Biotec #130-093-634 ...
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bioRxiv - Molecular Biology 2019Quote: Mitochondria were isolated from WT and NSUN2 KO HEK293T cells (1 × 107) using the Mitochondria Isolation Kit (Miltenyi Biotec). Steady-state levels of subunit proteins of mitochondrial respiratory chain complexes were analyzed by immunoblotting with Total OXPHOS Rodent WB Antibody Cocktail (1:250 ...